한국인에서 치주질환과 관상동맥질환의 관련성에 대한 염증표지자와 IL-1 유전자 다변성의 영향

정하나,정현주,김옥수,김영준,김주한,고정태,Jeong, Ha-Na,Chung, Hyun-Ju,Kim, Ok-Su,Kim, Young-Joon,Kim, Ju-Han,Koh, Jung-Tae 대한치주과학회 2004 Journal of Periodontal & Implant Science Vol.34 No.3

Recently epidemiologic studies have indicated that the patients with periodontitis may have increased risk of ischemic cardiovascular events, and have suggested the important roles of blood cytokines and acute reactant proteins in the systemic infection and inflammatory response. Periodontitis and coronary heart disease (CHD) may share the common risk factors and the genetic mechanism associated with interleukin(IL)-1A, B and RA genotype may be involved in the production of IL-1. This study was aimed to investigate the relationship between angiographically defined CHD and periodontitis as chronic Gram-negative bacterial infection and to determine whether the IL-1 gene polymorphism is associated in both diseases. Patients under the age of 60 who had undergone diagnostic coronary angiography were enrolled in this study. Subjects were classified as positive CHD (+CHD, n=37) with coronary artery stenosis more than 50% in at least one of major epicardial arteries, and negative CHD (-CHD, n=30) without significant stenosis. After recording the number of missing teeth, periodontal disease severity was measured by means of plaque index (PI), gingival index (GI), bleeding on probing (BOP), probing depth (PD), clinical attachment level (CAL), and radiographic bone loss around all remaining teeth. Gingival crevicular fluid (GCF) was collected from the 4 deepest periodontal pockets and assessed for cytokine ($IL-1{\beta}$, IL-6, IL-1ra, tumor necrosis $factor-{\alpha}$, and prostaglandin $E_2$). Additionally, blood CHD markers, lipid profile, and blood cytokines were analyzed. IL-1 gene cluster genotyping was performed by polymerase chain reaction and enzyme restriction using genomic DNA from buccal swab, and allele 2 frequencies of IL-1A(+4845), IL-1B(+3954), IL-B(-511), and IL-1RA(intron 2) were compared between groups. Even though there was no significant difference in the periodontal parameters between 2 groups, GCF level of $PGE_2$ was significantly higher in the +CHD group(p<0.05). Correlation analysis showed the positive relationship among PD, CAL and coronary artery stenosis(%) and blood $PGE_2$. There was also significant positive relationship between the periodontal parameters (PI, PD, CAL) and the blood CHD markers (leukocyte count, C-reactive protein, and lactic dehyrogenase). IL-1 gene genotyping showed that IL-1A(+3954) allele 2 frequency was significantly higher in the +CHD group compared with the -CHD group (15% vs. 3.3%, OR 5.118,p=0.043). These results suggested that periodontal inflammation is related to systemic blood cytokine and CHD markers, and contributes to cardiovascular disease via systemic inflammatory reaction. IL-1 gene polymorphism might have an influence on periodontal and coronary heart diseases in Korean patients.

치주병원균에 대한 유산균의 억제효과

정하나,오종석,김영준,정현주,Jeong, Ha-Na,Oh, Jong-Suk,Kim, Young-Jun,Chung, Hyun-Ju 대한치주과학회 1999 Journal of Periodontal & Implant Science Vol.29 No.1

This study was performed to evaluate the effect of hydrogen peroxide-producing Lactobacillus acidophilus V-20onthe replication of periodontal pathogens, Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis. When A. actinomycetemcomitans and P. gingivalis were incubated alone and in the combination with L. acidophilus V-20, the viable cell numbers of A. actinomycetemcomitans and P. gingivalis were compared between those cultures. The effect of S. mutans, E. durans, and L. lactis on the replication of A. actinomycetemcomitans and P. gingivalis was also evaluated. The change of periodontal indexes(probine depth, gingival index, GCF volume) and the viable cell numbers of A. actinomycetemcomitans and black pigmented bacteroides in subgingival plaque sample were evaluated following gargling of fermented milk made from L. acidophilus V-20 for 1 month on patients with periodontal disease in maintenance phase. In the mixed culture of L. acidophilus V-20 and A. actinomycetemcomitans or P. gingivalis, the replication of A. actinomycetemcomitans or P. gingivalis wascompletely inhibited. But in the mixed culture of P. gingivalis and hydrogen peroxide-nonproducing Lactobacillus casei, the viable ceil numbers of P. gingivaliswas not decreased when compared with the numbers in the mixed culture of P. gingivalis and L. acidophilus V-20. In the mixed culture of A. actinomycetemcomitans and S. mutans, E. durans, or L. lactis, the viable cell number of A. actinomycetemcomitans was not almost changed when compared with the numbers in the culture of A. actinomycetemcomitans alone. And in the mixed culture of P. gingivalis and E. durans or L. lactis, the viable cell numbers of P. gingivaliswas not almost changed compared with the counts in the culture of P. gingivalis alone. But the replication of P. gingivalis was completely inhibited in the mixed culture of P. gingivalis and S. mutans. When the change of periodontal indexes following gargling of fermented milk was compared with baseline, probing depth and gingival index were not changed, but GCF volume was significantly decreased(p<0.05). And when the viable ceil numbers of microorganisms in subgingival plaque sample were compared with baseline, total viable ceil number was almost unchanged and the viable cell numbers of A. actinomycetemcomitans and black pigmented bacteroides were significantly decreased(p<0.05). These results suggest that L. acidophilus V-20 inhibit the replication of A. actinomycetemcomitans and black pigmented bacteroides by the formation of hydrogen peroxide.

넙치 유래 viral hemorrhagic septicemia virus (VHSV, genotype IVa)에 대한 단클론 항체 생산

정하나,장민석,오명주,김위식,Jeong, Ha-Na,Jang, Min-Seok,Oh, Myung-Joo,Kim, Wi-Sik 한국어병학회 2017 한국어병학회지 Vol.30 No.2

Mouse monoclonal antibodies (MAbs) were produced by using viral hemorrhagic septicemia virus (VHSV, genotype IVa) as an immunogen, isolated from diseased olive flounder (Paralichthys olivaceus). Four hybridoma clones secreting MAbs against VHSV were established. The MAbs were recognized the nucleoprotein (MAb 4), phosphoprotein (MAb 1) and matrix protein (MAbs 2 and 3) of VHSV by western blot analysis. Among them, the MAbs 1 and 4 strongly reacted with the VHSV-infected FHM cells, but not normal FHM cells. In enzyme linked immunosorbent assay, the four MAbs reacted with the VHSV, but not different six fish viruses (infectious hematopoietic necrosis virus, hirame rhabdovirus, spring viraemia of carp virus, infectious pancreatic necrosis virus, marine birnavirus and nervous necrosis virus). These results indicate that the MAbs are useful for diagnosis of VHSV infection.

책임윤리학에 기초한 지구온난화 관련 초등 환경교육 교재 개발

정하나(Ha-Na Jeong),지준호(Chun-Ho Chi),이상원(Sang-Won Lee) 한국환경교육학회 2009 環境 敎育 Vol.22 No.3

The purpose of this study is to develop the elementary environmental textbook relevant to global warming based on responsibility ethics. To achieve the purpose, concrete study processes are as follows. First, the theory of responsibility ethics of Jonas was analyzed. Second, contents relevant to global warming on textbooks and guide books in the 7th elementary curriculum was analyzed through reference study. Third, the environmental textbook relevant to global warming for 5th and 6th grade in the elementary school was developed which was based on responsibility. The results of this study are as follows. First, responsibility ethics of Jonas has a lot of merit if it is applied to environment education. Second, according to the analysis into textbooks and guide books in the 7th elementary curriculum, the contents concerning global warming are very poor in both quantity and quality. Third, the elementary environmental textbook relevant to global warming for 5th and 6th grade students was developed. This textbook based on responsibility ethics will be expected to be efficient on the learning compared with subsidiary textbook in use.

다시마(Saccharina japonica) 배우체의 미동정 진균증

정하나 ( Ha-na Jeong ),오명주 ( Myung-joo Oh ),최성제 ( Sung-je Choi ),서정수 ( Jung-soo Seo ),박명애 ( Myoung-ae Park ),김위식 ( Wi-sik Kim ) 한국수산과학회 2017 한국수산과학회지 Vol.50 No.2

In 2015, white cottony tufts were observed on gametophytes of the kelp Saccharina japonica. Wet mount and histopathology examination revealed numerous fungal hyphae and mycelium around the gametophytes. The gametophytes surrounded by fungal hyphae were generally round and empty. A specific 610-bp fragment of the internal transcribed spacer (ITS)-5.8S rDNA-ITS gene of fungi was amplified by polymerase chain reaction and the nucleotide sequence showed 100% identity with those of Acremonium sclerotigenum, Acremonium sp. and Ascomycota sp. When fungus-infected gametophytes were mixed with healthy gametophytes, a high transmission rate (100%) resulted. This is the first report of mycosis of gametophytes in Korea.

추구-회피동기 수준에 따른 여가만족, 레크리에이션 전문화, 행복의 관계: 야구동호인을 대상으로

정하나 ( Hana Jeong ),황선환 ( Sunhwan Hwang ) 한국융합과학회 2022 한국융합과학회 국제학술대회자료집 Vol.2022 No.0

목적: 이 연구의 목적은 추구-회피동기 이론을 적용하여 야구동호인의 추구-회피동기 수준에 따른 여가만족과 레크리에이션 전문화, 행복에 어떠한 차이가 있는지 검증하고, 추구-회피동기 집단별 여가만족이 레크리에이션 전문화에 어떠한 영향을 미치는지 규명하는 것이다. 과정: 이를 위해 서울, 경기지역에서 활동하는 야구 참여자를 모집단으로 설정하고, 목적표집법을 이용하여 표본을 추출하였으며, 400명의 야구동호인을 대상으로 설문조사를 실시하였다. 응답이 누락되거나 일괄된 번호로 응답하거나 신뢰성이 낮다고 판단되는 자료를 제외한 총 380부의 설문지를 SPSS 27.0 프로그램과 AMOS 24.0 프로그램을 이용하여 분석하였다. 야구동호인의 추구-회피동기의 수준에 따른 집단 분류를 위해 추구-회피동기를 구성하는 다양한 하위요인 모델 중 개인적 추구, 대인적 추구, 개인적 회피, 대인적 회피동기로 구성된 하위요인 모델을 선정하고 ①형 추구-히피 동기로 명명하였다. 다음으로 추구동기, 회피동기, 개인적 동기, 대인적 동기로 구성된 모델을 선정하고 ②형 추구-회피동기로 명명하였다. ①형과 ②형으로 유형화된 각각의 추구-회피동기를 최종분석에 활용하였다. 결과: 첫째, ①형과 ②형으로 분류된 유형별 추구-회피동기 수준에 따른 여가만족의 차이 검증결과 추구-회피동기 수준에 따라 여가만족에 유의한 차이가 나타났다. 야구동호인에게 있어 추구-회피동기를 구성하는 요인들이 모두 높을 때 여가만족이 높은 것으로 나타났다. 둘째, ①형과 ②형 유형별 추구-회피동기 수준에 따른 레크리에이션 전문화의 차이 검증결과 추구-회피동기 수준에 따라 레크리에이션 전문화에 유의한 차이가 나타났으며, 특히 추구-회피동기의 모든 하위요인이 높을 때 레크리에이션 전문화 수준이 가장 높은 것으로 나타났다. 셋째, ①형과 ②형 유형별 추구-회피동기 수준에 따른 행복의 차이를 검증한 결과 유의한 차이가 없는 것으로 나타났다. 마지막으로 ①형과 ②형 추구-회피동기 수준에 의해 분류된 4개 집단별 여가 만족이 레크리에이션 전문화에 미치는 영향을 분석한 결과 여가만족은 레크리에이션 전문화에 유의한 영향을 미치는 것으로 나타났다. 추구-회피동기 수준에 따라 분류된 모든 집단에서 여가만족이 높을수록 레크리에이션 전문화가 높은 것으로 나타났다. 결론: 결과적으로 야구동호인의 여가만족, 레크리에이션 전문화에 있어 추구동기, 회피동기, 개인적·대인적 차원의 모든 하위요인이 중요한 요인으로 나타났으며, 여가만족과 레크리에이션 전문화 같은 여가경험을 지속시키기 위해서는 모든 차원의 추구-회피동기를 고려하여 욕구를 충족시킬 수 있는 여가환경을 조성하는 것이 중요하다. Purpose: verify the difference between leisure satisfaction, recreation specialization, and happiness according to the level of seeking and escaping motivation theory about baseball club members, and to verify the relationship between leisure satisfaction and recreation specialization. Method: A survey was conducted on baseball club members who participated in the baseball league, and a total of 380 people responded to the survey using the purposive sampling method. The data analyses were carried out using the SPSS 27.0 and AMOS 27.0 programs. Among the various sub-factor models constituting the seeking and escaping motivation, four sub-factor models consisting of personal seeking, interpersonal seeking, personal escaping, and interpersonal escaping motivation were selected and named type 1 seeking and escaping motivation. Next, a model consisting of four sub-factors: seeking motivation, escaping motivation, personal motivation, and interpersonal motivation was selected and named type 2 seeking and escaping motivation. Each seeking and escaping motivation categorized into type 1 and type 2 was used for analysis to verify the difference between leisure satisfaction, recreational specialization, and happiness. Result: As a result of the study, First, as a result of verifying the difference in leisure satisfaction according to the level of seeking and escaping motivation by type 1 and type 2, there was a significant difference in leisure satisfaction according to the level of seeking and escaping motivation. Second, as a result of verifying the difference in recreational specialization according to the level of seeking and escaping motivation by type 1 and type 2, there was a significant difference in recreational specialization according to the level of seeking and escaping motivation. Especially, when the level of seeking and escaping motivation for all sub-factor was high, the level of recreation specialization was found to be the highest. Third, as a result of verifying the difference in happiness according to the level of seeking and escaping motivation according to the level of seeking and escaping motivation by type 1 and type 2, no significant difference was found. As a results, the seeking and escaping motivation that explains the reason for participating in baseball proved to be a factor that can increase leisure satisfaction and recreational specialization. Next, a simple regression analysis was conducted to analyze the relationship between leisure satisfaction and recreational specialization of the four groups classified by type 1 and type 2 seeking and escaping motivation level. As a result of the implementation, it was found that the leisure satisfaction of the four groups of type 1 and type 2 classified by the level of seeking and escaping motivation had a significant effect on recreational specialization. Especially, it was found that the higher the leisure satisfaction of baseball club members, the higher the recreation specialization. Conclusion: As a result, all sub-factors of seeking-escaping (seeking motivation, escaping motivation, and personal and interpersonal motivation) levels appeared to be important factors in leisure satisfaction and recreation specialization of baseball club members. Therefore It is important to create a leisure environment that can satisfy needs by considering seeking-escaping motivation of baseball club members.

장애인 건강검진의 운영 및 이용 관련 요인

정하나(Ha Na Jeong),장선주(Sun Ju Chang),이경숙(Kyoung Suk Lee) 한국근거기반간호학회 2022 근거와 간호 Vol.10 No.1

Purpose: This study was aimed to identify facilitators and barriers related to management and utilization of health examinations for disabled adults. Methods: In the review, seven databases were searched for articles published from January 2017 to August 2022. Data was collected by dividing into management and utilization of health examinations for disabled adults. Then, data synthesis was conducted by subdividing the data into intrapersonal, interpersonal, and environmental factors. Results: A total of 29 articles were included. The facilitators related to health examinations were mild disability and experiences of high satisfaction with medical services as intrapersonal factors, skilled healthcare practitioners as interpersonal factors, and easy-to-understand materials as environmental factors. The barriers related to health examinations included severe disability and lack of awareness related to health examinations as intrapersonal factors, lack of therapeutic relationships with healthcare practitioners as interpersonal factors, and inconvenient equipment and facilities as environmental factors. Conclusion: Our findings could comprehensively provide information necessary to improve the management and utilization of general health examination, cancer screening, and dental examination by types of disabilities.

특수학교 자유학기제에 대한 특수교사의 인식, 기대효과 및 운영과제

정하나 ( Jeong Ha-na ),이영철 ( Lee Young-chul ) 대구대학교 특수교육재활과학연구소 2017 특수교육재활과학연구 Vol.56 No.2

이 연구는 특수학교 자유학기제 도입에 대한 특수교사의 인식, 기대효과 및 운영과제를 알아보는데 있었다. 연구대상은 부산광역시에 위치하고 있는 15개의 특수학교 중에서 장애유형을 고려하여 시각장애, 청각장애, 지적장애, 지체장애학교 각 1개교에서 중학교 수업을 지도하는 교사 150명을 편의목적표집을 하였다. 연구에서 얻은 결과는 다음과 같다 첫째, 특수학교 자유학기제에 대한 특수교사의 인식 결과는 다양하게 나타났다. 특수교사들은 전체적으로 자유학기제에 대해 잘 알고 있는 것으로 나타났으며, 자유학기제 연수 참여 경험이 있다고 응답한 특수교사들은 자유학기제의 연수의 내용이 도움이 되었는지에 대해 `보통이다`의 응답이 높게 나타났다. 특수학교에 바람직한 `자유학기 활동` 운영 모형과 `자유학기 활동` 연수는 진로탐색 활동, 주제선택 활동, 예술·체육 활동, 동아리 활동 순으로 나타났다. 특수학교 자유학기제 시행에 따른 특수교사의 가장 큰 역할에는 통합수업 운영을 통한 교과 간의 융합과 진로체험, 지역 인프라 탐색 및 적용이 가장 큰 역할이라고 인식하고 있었다. 둘째, 특수학교 자유학기제에 대한 특수교사의 기대효과는 학교생활의 만족도 증가와 긍정적인 학생의 정서 발달, 진로ㆍ직업교육 강화 등이었다. 셋째, 특수학교 자유학기제에 대한 특수교사의 운영과제는 담임업무 경감과 지역 인프라 확충, 특수학교에 표준화된 자유학기제 프로그램 개발 등이었다. The purpose of this study was to investigate recognitions, expected effectiveness and operational issues of special teacher on execution of special school free semester. The subjects from 15 special schools located in Busan metropolitan city were sampled. The subjects were sampled four special schools for visual, auditory, intellectual and physical disabilities. The sampled were 150 middle school teachers. Recognitions, expected effectiveness and operational issues of special teacher on execution of special school free semester were analyzed. The results of this study are summarized as follows. First, recognitions of special teacher on execution of special school free semester are well acquainted. There were many special teachers who answered that they had not received the experience in the free semester training, but the special teachers who answered that they had participated in the free semester training program. The answer of `usually` was high whether it was helpful or not. And it were recognized that the preferred period of the free semester in the special schools was the ` grade 1 semester 2` as the most desirable. The `Free Semester Activities` model, which is desirable for special schools, appeared in the order of career exploration activity, topic selection activity, arts and sports activities, and club activities. The `free semester activity` training required on execution of special school free semester were appeared in the order of career exploration activity, topic selection, arts and sports activities, and club activities. Namely, special school teachers preferred career exploration activity as `free semester activity`. The most important role of special education teachers on execution of special school free semester was recognized as the most important role of integrated class operating and exploring the local infrastructure for career experience. Second, expected effectiveness on execution of special school free semester, the increase of satisfaction of school life was the most expected effect and followed by positive student emotional development, career and vocational education. Third, operational issues on execution of special school free semester, reduction of teachers business, expansion of local infrastructure, and development of standardized free semester for special schools were the biggest operational issues. Through this study, free semesters in the special school is successful operation and activity requires to need a variety of special teacher training program. Depending on the type of special school, it may suggest that the program requires the development of a free semester for student characteristics.

공공데이터 품질진단 지원을 위한 진단규칙 매핑모델 제안

정하나(Ha-Na Jeong),김재웅(Jae-Woong Kim),이윤열(Yun-Yeol Lee),채의근(Yi-Geun Chae),정영석(Young-Suk Chung) 한국컴퓨터정보학회 2022 한국컴퓨터정보학회 학술발표논문집 Vol.30 No.2

데이터 분포 통계를 이용한 CSV 형식의 공공데이터 도메인 판별 모델에 관한 연구

정하나(Ha-Na Jeong),김재웅(Jae-Woong Kim),이윤열(Yun-Yeol Lee),채의근(Yi-Geun Chae),정영석(Young-Suk Chung) 한국컴퓨터정보학회 2023 한국컴퓨터정보학회 학술발표논문집 Vol.31 No.2