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Erythropoietin의 114-119 아미노산 잔기들의 제거 및 치환에 따른 생물학적 활성의 효과
정태완 嶺南大學校 基礎科學硏究所 1995 基礎科學硏究 Vol.15 No.-
Erythropoietin (EPO) is a glycoprotein hormone which regulates the proliferation and differentiation of erythroid progenitor cells. One of the earlier studies has suggested some important functional role of amino acids 99-129 in the hormone's biological activity, possibly because some amino acids in that region play critical role in the binding of the hormone protein to its receptor. In this study, we thus have generated two deletion of amino acids 117 through 119 through 119. To make the screening procedure easier, the deletion was accmpanied by insertion of Glu-Phe, which introduced the EcoRI restriction site at the deleted place. When the mutant and wild type EPO cDNAs were transfected into COS-7 cells and the biological activities of the muteins were assayed using the primary murine erythroid spleen cells, no deletion/substitution tested was found to significantly affect the biological activity of the hormone. The result presented here clearly shows that amino acids 114-119 in EPO are not critically involved in the receptor-binding.
정태완,박상일 (사)한국컴퓨터그래픽스학회 2012 컴퓨터그래픽스학회논문지 Vol.18 No.1
In this paper, we present a new method for automatically synchronize captured facial expression data with its corresponding motion data. In a usual optical motion capture set-up, a detailed facial expression can not be captured simultaneously in the motion capture session because its resolution requirement is higher than that of the motion capture. Therefore, those are captured in two separatesessions and need to be synchronized in the post-process to be used for generating a convincing character animation. Based on the patterns of the actor's neck movement extracted from those two data, we present a non-linear time warping method for the automatic synchronization. We justify our method with the actual examples to show the viability of the method. 본 논문은 동작 포착 장비를 통해 각각 따로 포착된 얼굴과 동작 데이터의 자동 동기화 기술에 대해 다룬다. 광학식 동작 포착 기기를 사용할 때 얼굴 표정과 동작의 포착은 별도로 이루어지는 경우가 많으며, 이 경우 두 데이터 간의 동기화 수행하여야 자연스로운 애니메이션을 만들 수 있다. 본 연구에서는 두 데이터 간의 공통 부분인 목 및 얼굴의 전체적인 움직임 데이터를 기준으로 비선형 시간 변형을 통해 동기화를 수행하는 기법을 제안한다. 연구 결과를 간단한 실험 시나리오에 적용하여 기술의 효과성 여부를 검증하였다.
견관절의 석회화 건염에 대한 초음파 유도하 다발성 천공술 및 고에너지 체외 충격파 병합치료
정태완,송동익,이순혁,정웅교,Jung, Tae Wan,Song, Dong Ik,Lee, Soon Hyuck,Jeoung, Woong Kyo 대한정형외과초음파학회 2014 대한정형외과 초음파학회지 Vol.7 No.1
목적: 견관절에 발생한 석회화 건염에 대해 초음파 유도하 다발성 천공술 및 고에너지 충격파 병합 치료의 유용성을 알아보고자 하였다. 대상 및 방법: 2010년 1월부터 2013년 6월까지 어깨 통증으로 내원하여 견관절 석회화 건염을 진단받은 환자 중 초음파 유도 하 다발성 천공술 및 고에너지 충격파 병합 치료를 받은 환자 42명을 대상으로 하였고 추시 기간은 평균 45주였다. 임상적 평가는 시술 전과 시술 12주 후의 통증에 대한 시각 점수 척도(pain visual analogue scale, P-VAS), ASES 점수(ASES score)와 UCLA 점수(UCLA score)를 사용하여 평가하였고, 초음파상 평가는 석회 침착의 크기와 상태를 비교하였다. 결과: 시술 후 통증에 대한 시각 점수 척도와 ASES 점수 및 UCLA 점수는 모두 통계적으로 의미 있게 호전되었으며(p<0.05), 침착된 석회는 61%에서 크기가 감소하였고, 27%에서 완전 혹은 거의 완전한 소실이 관찰되었다. 결론: 초음파 유도 하 다발성 천공술 및 고에너지 충격파 병합 치료는 견관절 석회화 건염에서 통증 및 임상 기능의 호전과 침착된 석회를 효과적으로 감소시킬 수 있는 유용한 방법으로 판단된다. Purpose: To evaluate the effectiveness of ultrasonography-guided combined multiple needling and high-energy extracorporeal shock wave therapy (ESWT) for calcific tendinitis of the shoulder. Materials and Methods: We included 42 calcific tendinitis patients who underwent ultrasonograpy-guided multiple needling followed by high-energy ESWT who visited the clinic from January 2010 to June 2013. The average follow up period was 45 weeks. Clinical evaluation was done before and after 12 weeks from treatment, in clinical terms using pain visual analogue scale (P-VAS), ASES, UCLA scores reflecting performance and symptom improvement, and in sonographic terms by studying the changes in size of the calcific nodules. Results: A statistically significant improvement was seen in P-VAS, ASES, UCLA scores and decreased calcification size on sonographic evaluation. Conclusion: Ultrasonography-guided combined multiple needling and high-energy ESWT is considered as a useful method which could provide clinical function improvement and reduction of calcification deposit.
Fatty acid catabolism in Escherichia coli
정태완 한국생화학분자생물학회 1990 생화학분자생물학회 소식 Vol.10 No.2
A great deal of information regarding the physiology, genetics, and molecular biology of fatty acid and acetate metabolism has been accumulated from studies with E. coli. The use of recombinant DNA technology, coupled with the purification and characterization of enzymes, have enable researchers to correlate the structural genes of the fatty acid degradation with their respective gene products. The synthesis of at least five fatty acid-oxidative (FAO) enzymes have been shown to be coordinately induced when long-chain and medium-chain fatty acids (LCFA and MCFA) are presept in the growth media. The structural genes encoding these FA0 enzymes have been mapped at several sites ,an the E. coli chromosome to make up fad regulon. This regulon is primarily responsible for the transport, acylation, and 8-oxidation of MCFAs and LCFAs. In addition to the FA0 enzymes, two degradative enzymes encoded by the atoA and atoB genes appear to be required for the growth on short-chain fatty acids (SCFAs). The glyoxylate shunt enzymes, which are required together with the FA0 enzymes for growth of E. coli on fatty acids and acetate, are encoded by ace operon. Among the three structural genes, ace A, ace B, and ace K, the last gene (ace K) codes for the isocitmte dehydrogenase (IDH) kinase-phosphatase, which controls the flow of isocitrate via the phosphorylatioddephosphorylation of the Krebs cycle enzyme, IDH. The fad system has been shown to be requlated by the fad R gene and double regulatory system, fad R and ici R genes, appear to be responsible for the control of glyoxylate shunt enzymes.
이숙영,정태완 嶺南大學校 基礎科學硏究所 1992 基礎科學硏究 Vol.12 No.-
In the first part of this series, we reported the constructions of four different mutants which resulted in disruption of the three AP 1 binding sites within the U3 region of Human Foamy virus(HFV) LTR. In the present study reported here, the plasmids carrying these mutant and wild type LTR sequences were introduced into BHK 21 cells by transfection and the chloramphenicol acetyltransferase activities(CAT) which were under the transcriptional control of those LTR sequences were determined. The plasmid carrying mutation in the most distal AP 1 site from the trancriptional start point showed only 10-15% CAT activity of that of the wild type when the viral transantivator bel 1 was coexpressed in the unstimulated cells. The other three mutations also gave significant loss of activities in directing the transcription of CAT gene under the same experimental condithons. In another set of experiment where a phorbol ester, PMA, was treated to the transfected cells to stimulate the production of AP 1 factor from the cells, significant increases in transcription activity of the wild type LTR, but not of mutant LTRs, were observed with increasing amounts of the stimulating agent. These results strongly suggest that the bel 1-mediated transactivation of viral gene expression is regulated through the AP 1 binding sites, presumably by some protein-protein intractions between AP 1 and bel 1 through the intervening of AP 1 binding sites.