통합 의학 치료로 5년 생존 및 완전 관해에 도달한 췌장암 증례 보고 1례

정유진,김지수,박경덕,오유나,정범진,방선휘,Yu-jin Jung,Jisoo Kim,Kyung-Dug Park,Yoona Oh,Beom-Jin Jeong,Sunhwi Bang 대한한방내과학회 2023 大韓韓方內科學會誌 Vol.44 No.3

Objectives: This is a five-year survival and complete response (CR) report on pancreatic cancer treated with western medicine and Korean traditional medicine. Method: A 59-year-old woman diagnosed with pancreatic cancer visited ○○ Korean traditional medicine hospital after neoadjuvant chemotherapy and pylorus-preserving pancreaticoduodenectomy. She was treated with Korean traditional medicine, including acupuncture, abdominal moxibustion, wild ginseng pharmacopuncture, and herbal medicine, which was based on integrated medicine therapy (IMT), from March 2018 to September 2022. The tumor size was measured by scanning with computed tomography (CT), magnetic resonance imaging, and positron-emission tomography/CT. Adverse events were evaluated using laboratory conclusion and National Cancer Institute Common Terminology Criteria for Adverse Events version 5.0. Result: During four years and three months of treatment, IMT maintained safety. The patient finally reached five-year survival without any recurrence or complication (CR) on October 26, 2022. Conclusion: We suggest that an integrative approach including Korean traditional medicine can be a meaningful treatment option for pancreatic cancer. Further studies should be performed to establish the proper treatment protocol of integrative medicine for pancreatic cancer.

식물 유성 생식과정에서 후성유전학적 정보해석 및 연구현황

정유진,조용구,강권규,Jung, Yu Jin,Cho, Yong-Gu,Kang, Kwon Kyoo 한국식물생명공학회 2017 식물생명공학회지 Vol.44 No.1

Rapid progress in epigenetic studies has resulted in genome wide information of genetic functions, other than DNA sequence information. However, insufficient understanding and unclear research direction in epigenetics has failed to attract many researchers. Here, we review the sexual reproduction processes that are particularly related to epigenetics in plants. We aim to elucidate the roles of epigenetic information and molecular mechanisms involved in the complex sexual reproduction process of plants, and examine their biological significance.

제주산 파인애플 유래 Bromelain관련 유전자 (BL1)를 이용반 형질전환 상추의 특성

정유진,김기훈,최장선,이순열,노일섭,박진희,강권규,Jung, Yu-Jin,Kim, Gi-Hyun,Choi, Jang-Sun,Lee, Soon-Youl,Nou, Il-Sup,Park, Jin-Heui,Kang, Kwon-Kyoo 한국식물생명공학회 2006 식물생명공학회지 Vol.33 No.1

파인애플 (Ananas comosus) 줄기에서 얻어지는 bromelain은 단백질 분해효소 중 cysteine protease의 복합체로 알려져 있다. 본 연구에서는 제주산 파인애플 줄기를 이용하여 bromelain 관련 유전자를 분리하였다. 분리된 BL1 유전자는 총 933개의 염기서열로 311개의 아미노산을 coding 하였다. 지금 까지 알려진 식물 유래 bromelain 관련 유전자와의 alignment 분석한 결과 BAA21929 유전자와 94%, T10516 유전자와 93% 및 P14518 유전자와 81%의 상동성을 보였다. BL1 유전자를 상추 게놈내에 도입하고자 NPTII 유전자 와 BL1 유전자로 제작한 pBI 121 BL 벡터를 Agrobacterium tumefacience LBA4404에 도입한 후, 상추잎 절편에 감염시켜 embryogenic callus 및 재분화 식물체를 육성하였다. 이들식물체로부터 T1세대를 육성하여 PCR 분석을 통해 왜래유전자의 도입 여부를 확인하였다. 또한 형질전환체의 발현여부는 Nothern blot분석 및 eno protease활성을 통해 형질전환체에서 BL1유전자가 안정적으로 상추세포내에서 발현되고 있음을 확인하였다. 따라서 본 실험에서 육성된 bromelain 관련 BL1 유전자가 도입한 형질전환 상추를 육종소재르 활용한다면 상업적으로 유용한 단백질을 분해하는 가수분해효소로써 건강 보조제, 사료첨가제 등에 널리 사용할 수 있을 것으로 생각되어진다. To clarify the roles of bromelain in plants, we isolated BL1 gene encoding bromelain from pineapple stem tissues and sequenced. The full length cDNA is 933 bp and encodes a polypeptide of 311 amino acid residues. The cDNA is most similar 94% at the amino acid level to bromelain previously isolated from pineapple (BAA21929). Explants of Lactuca sativa were co-cultivated with Agrobacterium tume-faciences LBA 4404 strains containing nptII and BL1 gene for transformation. Through initial selection of regenerated explants by culturing on a kanamycin and carbenicillin containing MS medium, multiple shoots were obtained after 2 months of culture. For a complementary step of selection, putative transgenic shoots were transferred to 1/2 Ms basal medium supplemented with 100 mg/L kanamycin and 500 mg/L carbenicillin. The selected shoots were obtained T1 generation seeds with emasculation, and tested with PCR analysis using 35S promoter and BL1 specific primers whether BL1 gene was introduced to genome of the plants. These results confirmed that produced the specific PCR bands in the putative transgenic lines. Additionally the Northern blot and endo protease activity showed that transcripts of BL1 gene were detected in transgenic lines. Theses results suggest that BL1 gene be successfully integrated and transcripted in the transgenic lettuce plants.

무용전공고등학생의 경쟁불안이 무용몰입 및 무용성취에 미치는 영향

정유진,민윤미,Jung, Yu-Jin,Min, Yun-Mi 한국디지털정책학회 2020 디지털융복합연구 Vol.18 No.9

이 연구는 무용전공고등학생의 경쟁불안이 무용몰입 및 무용성취에 미치는 영향을 규명하는데 목적을 두고 진행되었다. 연구대상은 2020년 현재 서울 및 수도권 소재 고등학교에서 무용을 전공하고 있는 학생이었으며, 조사도구는 설문지를 사용하여 온라인 설문을 실시하였다. 총 222명의 자료를 Windows용 SPSS 18.0을 통해 t-test, 일원변량분석, 상관분석, 다중회귀분석 및 위계적 회귀분석을 적용하여 자료 처리한 결과 다음과 같은 결론이 도출되었다. 첫째, 남학생이 여학생보다 상태자신감이 높고, 집안의 경제력이 중하위권이라고 인지하는 학생들의 인지불안이 높았다. 또한 상태자신감과 무용성취는 경제력이 상위권이라고 인지하는 집단이 중하위권보다 높은 것으로 나타났다. 둘째, 인지적 불안과 상태자신감은 무용몰입에 정적영향을 미치는 것으로 나타났다. 셋째, 인지적 불안과 상태자신감은 무용성취에 정적영향을 미치는 것으로 나타났다. 넷째, 무용몰입은 경쟁불안과 무용성취에 관계된 강력한 매개변인으로 나타났다. 이상의 결론을 종합해 볼 때, 무용전공 고등학생들의 경쟁불안은 무용에 몰입하는데 작용하며 무용 성취를 높이는 것으로 나타났으나, 이와 관련된 부작용이 있을 것으로 예견되는바 후속 연구에서는 이러한 심리적 변인들에 대한 추가 연구가 필요한 것으로 판단된다. The study was conducted with the aim of identifying the impact of competition anxiety on dance immersion and performance in high school students majoring in dance. As of 2020, online surveys were conducted on students majoring in dance at high schools in Seoul and the metropolitan area. A total of 222 people's data were processed by applying t-test, one-way variable analysis, correlation and multiple regression analysis through SPS 18.0 for Windows, resulting in the following conclusions. First, male students were more confident in their condition than female students, and students who recognized that their family's economic power was in the middle class were more aware. In addition, it was found that the group perceived that economic power was at the top of the list was higher than the median. Second, cognitive anxiety and self-confidence have been shown to have a static effect on immersion into dance. Third, cognitive anxiety and self-confidence have been shown to have a static effect on the achievement of uselessness. Fourth, immersion in dance appeared to be a powerful parameter related to competition anxiety and achievement of dance. To sum up the above conclusions, competition among high school students majoring in dance has been shown to increase their performance by immersing themselves in dance, but further studies of these psychological variables are expected to have side effects.

벼에서 CRISPR/Cas9 활용 고빈도 유전자 편집 방법

정유진,배상수,이긍주,서필준,조용구,강권규,Jung, Yu Jin,Bae, Sangsu,Lee, Geung-Joo,Seo, Pil Joon,Cho, Yong-Gu,Kang, Kwon Kyoo 한국식물생명공학회 2017 식물생명공학회지 Vol.44 No.1

CRISPR/Cas9 기술은 생명공학을 활용한 신품종 작물육성에 있어 패러다임 변혁을 가져다 줄 핵심 기반기술이다. 본 연구에서는 CRISPR/Cas9를 이용하여 유전자편집기술을 기존에 알려진 방법보다 쉽고 정확하게 실험 할 수 있도록 sgRNA 디자인, 벡터구축, 형질전환체 육성 및 분석 등을 자세히 기술하였다. sgRNA는 http://www.rgenome.net/ 사이트에서 NGG 영역을 중심으로 하여 target-up: 5'-ggcaGNNNNNNNNNNNNNNNNNNNN-3'과 target-down: 5'-aaacNNNNNNNNNNNNNNNNNNNNC-3'의 올리고를 디자인하였다. 식물형질전환용 벡터는 pPZP-Cas9-RGEN을 기본으로 하였으며, sgRNA의 프로모터는 OsU3를 이용하여 pPZP::35S::Cas9::PinII-OsU3::sgRNA::Bar-Gen 순으로 구축하였다. 형질전환체의 육성은 단기형질전환 Agrobacterium 법을 사용하였으며 재분화 식물체를 얻는데48일 정도 소요되었다. 형질전환체 유무는 genomic PCR 분석으로 single copy 선발은 TaqMan PCR로 분석하였다. 정밀유전자편집 식물체는 T1 세대에서 T-DNA 삽입되지 않은 식물체를 Bar-strip에 의해 선발하였다. 선발된 식물체의 sgRNA 영역의 염기배열 조사에 의해 유전자 편집 식물체를 육성하였다. 따라서 본 연구에서 CRISPR/Cas9 system에 의한 정밀유전자편집 기술을 이용하여 보다 빠르고 쉽고 경제적으로 유전자가 편집된 개체를 확보할 수 있었다. 본 실험에서 확립된 system은 상업용 식물 계통육성에 이용 가능하여 육종적 가치가 매우 클 것으로 사료된다. The CRISPR/Cas9 is a core technology that can result in a paradigm for breeding new varieties. This study describes in detail the sgRNA design, vector construction, and the development of a transgenic plant and its molecular analysis, and demonstrates how gene editing technology through the CRISPR/Cas9 system can be applied easily and accurately. CRISPR/Cas9 facilitates targeted gene editing through RNA-guided DNA cleavage, followed by cellular DNA repair mechanisms that introduce sequence changes at the site of cleavage. It also allows the generation of heritable-targeted gene mutations and corrections. Here, we present detailed procedures involved in the CRISPR/Cas9 system to acquire faster, easier and more cost-efficient gene edited transgenic rice. The protocol described here establishes the strategies and steps for the selection of targets, design of sgRNA, vector construction, and analysis of the transgenic lines. The same principles can be used to customize the versatile CRISPR/Cas9 system, for application to other plant species.

박탈성 피부염의 한방 치험 1례

정유진,윤화정,고우신,Jung, Yu-Jin,Yoon, Hwa-Jung,Ko, Woo-Shin 대한한방안이비인후피부과학회 2014 한방안이비인후피부과학회지 Vol.27 No.3

Objective : The purpose of this report is to know the effect of Korean medical treatments on exfloliative dermatitis. Methods : We treated a 25-years-old man patient with exfloliative dermatitis on the limbs and back with Korean medicine. After Korean medical teatment for 26 days, we measured the decrease of dermatitis and recovery of skin exfloliate. We recorded pictures of changes on symptoms. Result & Conclusion : The symptoms of exfloliative dermatitis were significantly improved. The dry skin and itching were disappeared and skin damages were almost recovered. Thus Korean medical treatments are effective on exfloliative dermatitis.

Single Interaction Force of Biomolecules Measured with Picoforce AFM

정유진,박준원,Jung, Yu-Jin,Park, Joon-Won The Korean Vacuum Society 2007 Applied Science and Convergence Technology Vol.16 No.1

생명현상 발현에 중요한 역할을 하는 생체 분자간 특이적 상호작용을 단분자 수준에서 이해하려는 연구는 매우 중요한 일이다. 나노 바이오 측정기술을 이용하여 여러 복잡한 생명현상을 그 기본 단위인 단일 세포 차원에서 직접 측정하여 응용하려는 시도가 이루어지고 있다. 이런 시도로써, 원자힘 현미경을 이용한 생체분자간의 결합력 측정은 생명현상과 가장 유사한 환경에서 단일 생체 분자간 또는 분자 내 힘을 직접 측정함으로써, 단일 생체분자의 현상을 관찰 할 수 있다는 장점을 가지고 있다. 특히 단분자 힘 분광학을 이용한 단일 생체분자내의 세부 단위체간 상호작용에 대한 연구와 단백질-단백질, 단백질-리간드, DNA-DNA의 분자인지 상호작용에 대한 연구는 많은 생명과학 분야 연구자들의 관심을 끌고 있을 뿐만 아니라 더 나아가 새로운 관련 기술의 개발을 촉진시키고 있다. The interaction force between biomolecules(DNA-DNA, antigen-antibody, ligand-receptor, protein-protein) defines not only biomolecular function, but also their mechanical properties and hence bio-sensor. Atomic force microscopy(AFM) is nowadays frequently applied to determine interaction forces between biological molecules and biomolecular force measurements, obtained for example using AFM can provide valuable molecular-level information on the interactions between biomolecules. A proper modification of an AFM tip and/or a substrate with biomolecules permits the direct measurement of intermolecular interactions, such as DNA-DNA, protein-protein, and ligand-receptor, etc. and a microcantilever-based sensor appeared as a promising approach for ultra sensitive detection of biomolecular interactions.

멜라닌 및 색소 이상 질환과 장부(臟腑)와의 연관성 고찰

정유진,고우신,윤화정,Jung, Yu-Jin,Ko, Woo-Shin,Yoon, Hwa-Jung 대한한방안이비인후피부과학회 2016 한방안이비인후피부과학회지 Vol.29 No.3

Objectives : The purpose of this research is to understand melanin with both Korean and Western medicine.Methods : We investigated the comprehension of melanin in both western and Korean medicine through literature review and studied relationships between melanin and five viscera(五臟), especially liver(肝), spleen(脾), kidney(腎). We Also studied representative pigmentary disorders(melasma, vitiligo) in western and Korean medicine to figure out how to understand pigmentary disorders in oriental medicine.Results : The results are as follows. 1. Melanin is associate with liver, because free coursing(疎泄) function of liver is the origin of transport melanin to keratinocyte from melanocyte. Also, melanogenesis factors like MITF and CREB are closely associated with liver and pigmentary disorders occur frequently after stress conditions or women. 2. Melanin is absorbed and scattered in keratinocytes by the function of spleen. Pigmentary disorders result from failure of spleen and formation of phlegm-retained fluid(痰飮). 3. Kidney essence(腎精) is the origin of melanin formation. In addition, corticosteroid, the major hormone of melanogenesis is secreted by adrenalin and adrenalin belongs to kidney(腎) in Korean medicine. 4. Melasma is created by disorder of melanin transport and absorbtion, so melasma is associated liver (肝) and spleen(脾). Therefore the treatment for melasma may focus on improvement function of liver and spleen. 5. The destruction of melanocyte or abnormal melanogenesis by disorder of the immune system, metabolic and affective disorders can make vitiligo, so vitiligo is associated with liver and kidney which are major part of melanin formation. Therefore the treatment of vitiligo can focus on improvement function of liver(肝) and kidney(腎).Conclusion : We compared Korean and western medicine to understand melanin. We also interpreted the mechanism of melanin and pigmantary disorders in western medicine and considered the relationship with visceral manifestation theory(臟象論) in traditional Korean medicine. Further studies are needed to apply comprehension of melanin to clinical stage.

신식물육종기술의 현황과 사회적 수용을 위한 노력

정유진,김종미,박수철,조용구,강권규,Jung, Yu Jin,Kim, Jong Mi,Park, Soo-Chul,Cho, Yong-Gu,Kang, Kwon Kyoo 한국식물생명공학회 2018 식물생명공학회지 Vol.45 No.4

Although new plant breeding technologies facilitate efficient plant breeding without introducing a transgene, they are creating indistinct boundaries in the regulation of genetically modified organisms (GMOs). The rapid advancement in plant breeding by genome-editing requires the establishment of a new global policy for the new biotechnology, while filling the gap between process-based and product-based GMO in terms of regulations. In this study recent developments in producing major crops using new plant breeding technologies were reviewed, and a regulatory model that takes into account the various methodologies to achieve genetic modifications as well as the resulting types of mutation were proposed. Moreover, the communication process were discussed in order to understand consumers' current situation and problems of new plant breeding technology, establish social acceptance well, and understand consumers' disputes such as GMO crops.

나이관련 황반변성에 대한 동서의학적 고찰

정유진,고우신,윤화정,Jung, Yu-Jin,Ko, Woo-Shin,Yoon, Hwa-Jung 대한한방안이비인후피부과학회 2015 한방안이비인후피부과학회지 Vol.28 No.3

Object : The purpose of this study is to understand age-related maculardegeneration(AMD) with both western and Korean medicine.Methods : We investigated the comprehension of general AMD degenation in both western and Korean medicine through literature review.Results : The results are as follows.1. AMD prevalent increasing as the population ages; however, treatment options remain limited and incompletely defined.2. Generally macular degeneration has been affected by aging and is associate with the function of kidney(腎) and liver(肝) in Korean medicine.Conclusion : Further studies are needed to apply comprehension of AMD in Korean medicine to clinical stage.