한국 헌혈자에서의 B형, C형 간염 표지자 양성율

정보찬,이선호,김두성,김상인 大韓輸血學會 1994 大韓輸血學會誌 Vol.5 No.2

실온노추링 혈액제제내 세균성장에 미치는 영향

정보찬,이재훈,김경동,김정숙,송달효 大韓輸血學會 1993 大韓輸血學會誌 Vol.4 No.1

공기 및 습윤 노출이 요시험지봉검사에 미치는 영향

정보찬,김경동,김정숙,이채훈 대한임상병리학회 1993 대한임상병리학회지 Vol.13 No.1

Comparison of 3 Phenotypic-detection Methods for Identifying Plasmid-mediated AmpC β-lactamase-producing Escherichia coli, Klebsiella pneumoniae, and Proteus mirabilis Strains

이우근,정보찬,홍성근,송원근,정석훈,이경원,곽효선 대한진단검사의학회 2009 Annals of Laboratory Medicine Vol.29 No.5

Background : Plasmid-mediated AmpC β-lactamases (PABLs) have been detected in the strains of Escherichia coli, Klebsiella spp., Proteus mirabilis, and Salmonella spp. PABLs may be difficult to detect and might interfere in the therapeutic and infection-control processes. Although several PABL-detection methods based on phenotypes have been reported, the Clinical and Laboratory Standards Institute currently does not recommend a routine detection method for PABLs. The aim of this study is to compare the performances of 3 phenotypic PABL detection methods. Methods : Total 276 non-duplicated clinical isolates of E. coli (N=97), K. pneumoniae (N=136), and P. mirabilis (N=43) were collected from 14 hospitals in Korea between April and June 2007 in a non-consecutive and non-random manner. Multiplex PCR was performed to detect the PABL genes. Further, 3 phenotypic detection methods-cephamycin-Hodge test, Tris-EDTA (TE) disk test, and combination-disk test with 3-aminophenylboronic acid (BA)-were performed using cefoxitin and cefotetan disks. Results : PABL genes were detected by multiplex PCR in 122/276 isolates, including 14/97 E. coli, 105/136 K. pneumoniae, and 3/43 P. mirabilis isolates. The combination-disk test with BA showed higher sensitivity (98.4%), specificity (92.2%), and efficiency (96.3%) than the cephamycin-Hodge (76.2%, 96.1%, and 88.6%, respectively) and the TE-disk (80.3%, 91.6%, and 87.9%, respectively) tests. Conclusions : The combination-disk test with BA is a simple, efficient, and interpretable test that can be applicable in clinical laboratories involved in the detection of PABLs in clinical isolates of E. coli, K. pneumoniae, and P. mirabilis. Background : Plasmid-mediated AmpC β-lactamases (PABLs) have been detected in the strains of Escherichia coli, Klebsiella spp., Proteus mirabilis, and Salmonella spp. PABLs may be difficult to detect and might interfere in the therapeutic and infection-control processes. Although several PABL-detection methods based on phenotypes have been reported, the Clinical and Laboratory Standards Institute currently does not recommend a routine detection method for PABLs. The aim of this study is to compare the performances of 3 phenotypic PABL detection methods. Methods : Total 276 non-duplicated clinical isolates of E. coli (N=97), K. pneumoniae (N=136), and P. mirabilis (N=43) were collected from 14 hospitals in Korea between April and June 2007 in a non-consecutive and non-random manner. Multiplex PCR was performed to detect the PABL genes. Further, 3 phenotypic detection methods-cephamycin-Hodge test, Tris-EDTA (TE) disk test, and combination-disk test with 3-aminophenylboronic acid (BA)-were performed using cefoxitin and cefotetan disks. Results : PABL genes were detected by multiplex PCR in 122/276 isolates, including 14/97 E. coli, 105/136 K. pneumoniae, and 3/43 P. mirabilis isolates. The combination-disk test with BA showed higher sensitivity (98.4%), specificity (92.2%), and efficiency (96.3%) than the cephamycin-Hodge (76.2%, 96.1%, and 88.6%, respectively) and the TE-disk (80.3%, 91.6%, and 87.9%, respectively) tests. Conclusions : The combination-disk test with BA is a simple, efficient, and interpretable test that can be applicable in clinical laboratories involved in the detection of PABLs in clinical isolates of E. coli, K. pneumoniae, and P. mirabilis.

한국 헌혈자에서의 Human Immunodeficiency Virus 감염 양성율 연구

이선호,정보찬,김두성,오영철 大韓輸血學會 1994 大韓輸血學會誌 Vol.5 No.1

한국인 산모에서 다운증후군 산전선별검사로서의 통합선별검사의 유용성 평가

이대영,정보찬,강명서,허지영,백진영,차동현,강석호 대한진단검사의학회 2011 Laboratory Medicine Online Vol.1 No.1

Background: Antenatal screening for Down’s syndrome has been developed and improved over the past 20 yr. Recently, integrated test, which combines the first and second trimester markers has shown the highest detection rate (DR) and lowest false positive rate (FPR) among Down’s syndrome screening tests currently in use. The purposes of this study were to evaluate the screening performance of integrated test and to compare the results with triple test studies in Korea. Methods: The study population consisted of Korean pregnant women who underwent triple or integrated test between April 2005 and December 2008. Triple test was performed using measurements of alpha-fetoprotein (AFP), unconjugated estriol (uE3), and human chorionic gonadotropin (hCG)in the second trimester. Integrated test was performed using nuchal translucency (NT) by ultrasonography and pregnancy-associated plasma protein A (PAPP-A) from maternal serum in the first trimester, and AFP, uE3, hCG, and inhibin-A in the second trimester. The screening performance of each test was evaluated by DR and FPR. Results: Twenty-seven Down’s syndrome pregnancies were confirmed in women screened by triple (N=6,736) or integrated test (N=7,688). At 1:100, 1:270, and 1:300 of risk cutoff, triple test showed 45%, 73%, and 73% of DR and 4.7%, 11.2%, and 12.4% of FPR, respectively. At 1:100,1:150, and 1:300 of risk cutoff, integrated test showed 63%, 69%, and 75% of DR and 1.5%, 1.9%, and 3.0% of FPR, respectively. Conclusions: Integrated test showed higher DR and lower FPR, demonstrating better screening performance than triple test.

Multistix-SG와 Comgur-9-test RL에 의한 요시험지봉검사 성적의 비교

김정숙,정보찬,김경동,김대철,조길호 영남대학교 의과대학 1991 Yeungnam University Journal of Medicine Vol.8 No.1

최근 널리 사용되고 있는 제조회사가 다른 두가지 요시험지봉(??)으로 요검사를 실시하면, 그 결과치의 표현에 있어 차이가 있으므로, 결과치 판정시 질병의 진단이나 치료의 효과 판정 및 경과관찰에 있어서 발생할 수 있는 혼란을 줄이고자, 환자검체와 표준검체에서 실시한 요시험지봉 검사결과를 비교 검토하여 아래와 같은 결론을 얻었다. 또한 두 시험지봉검사에 결과를 교환함에 있어 상응하는 결과치도 조사하였다. Combur-9-test RL 및 Multistix-SG를 이용하여, 1990년 12월 16일 부터 15일간 영남대학교 의과대학 부속병원 임상병리과에 요검사가 의뢰된 501명의 환자검체를 대상으로 하여 요시험지봉으로 화학적검사를 반정량적으로 시행한 결과 대체로 ±1등급 범위내에서 일치하였다. Combur-9-test RL을 이용한 leukocyte esterase검사와 요침사검사간의 비교에서 현미경적 농뇨와의 일치율은 83.7%, 민감도 48.1%, 특이도 90.3%, 양성예측율 47.4% 및 음성예측율 90.5%로 나타났다. Combur-9-test RL을 사용한 nitrite검사와 요배양검사간의 비교에서 요배양 양성과의 일치율은 93.0%, 민감도 19.4%, 특이도 84.7%, 양성예측율 53.8% 및 음성예측율 94.1%로 나타났다. 단백의 주 성분이 알부민 및 글로부린인 검체에서 실시한 요단백검사에서는 양자 모두에서 sulfosalicylic acid 법이 가장 민감하였고, 다음은 Multistix-SG, Combur-9-test RL의 순이었다. 요빌리루빈 및 요당검사에서는 두시험지봉이 비슷한 민감도를 나타내었다. 요중 백혈구검사에서는 요침사검사가, 요중잠혈검사에서는 Combur-9-test RL이 가장 민감하였다. Two types of urine dipstick assays. Multistix-SG and Combur-9-test RL, were compared for compatibility, accuracy, specificity and predictive values of a positive and negative test in 501 patients urine and artificially prepared specimen. We found that the results of semiquantitative tests of Multistix-SG and Combur-9-Test RL performed were statistically similar in patients specimen. The urinary leukocyte estrase tests of Combur-9-Test RL assays compared with urine sediment microscopy in regard to compatibility, sensitivity, specificity, and predictive values of a positive and negative test 83.7%, 48.1%, 90.3%, 47.4% and 90.1%, respectively. The urinar nitrite tests of Comber-9-Test RL assays compared with urine culture tests, in regard to compatibility, sensitivity, specificity, and predictive values of a positive and negative tests were 90.3%, 19.4%, 84.7%, 53.8% and 94.1%, respectively. For the urinary protein, the sulfosalicylic acid method was the most sensitive test for any kinds of protein, and Multistix-SG appeared more sensitive than Compur-9-Test RL for the albuminuria. For the urinary bilirubin and glusose, two dipstick assays were similar in their diganostic efficiency. Finally in the urinary occult blood tests, Combur-9-Test RL assays was more sensitive than Multistix-SG.

혈청 CK및 LD Isoenzyme 검사의 전산해석식 보고시스템

김대철,정보찬,조길호,김경동,김정숙 대한임상병리학회 1991 대한임상병리학회지 Vol.11 No.2

임상 검체에서 단독으로 분리된 Leclercia Adecarboxylata 2예

이우근,이대영,정보찬,허지영,강명서,홍성근,홍성관 대한감염학회 2009 감염과 화학요법 Vol.41 No.2

Leclercia adecarboxylata is a facultative gram negative bacillus of the Enterobacteriaceae family, It has been previously reported as a rarely isolated opportunistic pathogen, mainly in the form of mixed infection with other organisms. We report two cases of independent infection by L. adecarboxylata. One strain of L. adecarboxylata was isolated from Baker's cyst in an immunocompetent patient and the other strain from dialysate in a patient on continuous ambulatory peritoneal dialysis.

A Case of Adult B Lymphoblastic Leukemia with ider(9)(q10)t(9;22)(q34;q11.2) and der(19)t(1;19)(q23;p13.3)

정순일,조희순,이채훈,정보찬 대한진단검사의학회 2010 Annals of Laboratory Medicine Vol.30 No.6

In B lymphoblastic leukemia/lymphoma (B-ALL/LBL), t(9;22)(q34;q11.2) and t(1;19)(q23;p13.3) are recurrent cytogenetic abnormalities. The concurrent occurrence of both abnormalities is very rare, and only 3 cases have been previously reported. Here, we report a case of adult B-ALL with ider(9)(q10)t(9;22)(q34;q11.2) and der(19)t(1;19)(q23;p13.3). A literature review revealed that ider(9) (q10)t(9;22) is a rare variant of t(9;22) with a deletion of the short arm of chromosome 9. Fifteen cases of ider(9)(q10)t(9;22) have been reported. This abnormality is specific to precursor B-lymphoid neoplasms,such as B-ALL or B-lymphoid blast phase of CML, and is associated with disease progression or short survival. The cytogenetic abnormality t(1;19) is also specific to B-ALL. In most instances of t(1;19), TCF3 is fused to PBX1; however, a few cases have identical translocations but no TCF3-PBX1 fusion, as was observed in our patient. We describe the first case of ider(9)(q10)t(9;22) in combination with TCF3-PBX1 negative t(1;19). The patient underwent imatinib therapy in addition to intensive chemotherapy, but failed to achieve remission. (Korean J Lab Med 2010;30:585-90)