Clinical relevance of the CA 125 assay in monitoring of Epithelial ovarian cancer patients

정민형,이선경 대한산부인과학회 2003 대한산부인과학회 학술대회 Vol.89 No.-

백서 조골세포주에서의 싸이클로스포린 산소유리기 유도능

정민형,이태화,오영림,이지영,양승오,최웅환,김흥열 대한골다공증학회 2010 Osteoporosis and Sarcopenia Vol.8 No.2

Objectives: This study examined the ability of Cyclosporine (CsA) to induce apoptosis in a rat osteoblast cell line. Methods: Rat osteoblast ROS 17/2.8 cells were cultured, and treated with with 0.1~40μg/mL CsA for 24 hours after plating of cells. Cell viability was determined by the MTT assay. Western Blot Analysis was done with primary antibodies to caspase-3 and caspase-8. Reactive oxygen species (ROS) synthesis was measured by flowcytometry. Results: Cell viability decreased in dose-dependent manner with increasing concentrations of CsA. Treatment of ROS 17/2.8 cells with 0.1, 0.5, 1, 5, 10, 20, or 40 μg/mLg/mL CsA caused 85%, 80%, 73%, 60%, 45%, 40%, and 27% cell viability, respectively. Western blot analysis showed reduced caspase-3 expression and induced caspase-8. The ROS in a dose-and time-dependent manner were increased by CsA. Conclusion: These results suggest that CsA can induce oxygen free radicals which appears to trigger apoptosis by activating pro-apoptotic signals. CsA plays a role in the post- transplatation bone diseases via the induction of apoptosis in osteoblast.

Lethal and sublethal effects of synthetic insecticides on the locomotory and feeding behavior of Riptortus pedestris (Hemiptera: Alydidae) under laboratory conditions

정민형,김수완,김홍근,이두형 한국응용곤충학회 2018 Journal of Asia-Pacific Entomology Vol.21 No.1

The bean bug, Riptortus pedestris (Hemiptera: Alydidae) is a polyphagous insect pest and has a wide range ofhosts including leguminous plants and tree fruits. Currently, the management of R. pedestris mainly relies on theuse of insecticides, and most studies have focused on the lethal effects of insecticides. However, insecticides cannot only kill insects directly, but can also affect behavioral changes of survivors when exposed to sub-lethaldoses. In this study, we investigated locomotory behaviors (vertical movement, horizontal movement, and flightability) and feeding behaviors (frequency of insects approaching dried soybean seeds and number of styletsheaths left on the dried soybean seeds by insects) of surviving R. pedestris pre-exposed to five insecticide residuesfor 4 h. None of the three insecticides (bifenthrin, etofenprox, and acetamiprid) tested had significanteffects on the locomotory behaviors of R. pedestris adults compared to the water-treated control group. Fenitrothion- and dinotefuran-treated groups showed a significant decrease in the vertical movement comparedto the water-treated control, but the insects recovered mobility 24 h after the initial exposure. The frequency ofR. pedestris approaching to dried soybean seeds was affected by four insecticides (fenitrothion, etofenprox, bifenthrin,and dinotefuran), but the actual feeding activity of R. pedestris determined by the stylet sheaths remainingon the dried soybean seed was only affected by fenitrothion treatment. Given the relatively low toxiceffects of five insecticides tested, a better understanding of the impact of insecticides on the behavior of targetspecies is needed for a more robust pest control strategy and a more effective use of insecticides in IPM programs.

편의시설 기반 충전소 안내 앱

정민형,조진석,장기윤,이승형,이성철 한국정보처리학회 2022 한국정보처리학회 학술대회논문집 Vol.29 No.2

고래불 해안사구에서 염생식물의 공간분포

정민형,김석철,홍보람,이규송 한국환경생물학회 2017 환경생물 : 환경생물학회지 Vol.35 No.3

Factors affecting spatial distribution of halophytes were analyzed in June 2012 at the Goraebul coastal dunes. In the Goraebul sand dune, distribution of halophytes was divided into three groups. The first group belonging to Elymus mollis, Carex kobomugi, Calystegia soldanella, Ixeris repens and Glehnia littoralis was distributed in the ridge of primary sand dune and dune slack. The second group belonging to Lathyrus japonicus and Zoysia macrostachya was distributed in the dune slack. The third group belonging to Pinus thunbergii, Vitex rotundifolia and Linaria japonicus was distributed in the pine forest of the secondary sand dune. E. mollis, C. kobomugi, C. soldanella, I. repens and G. littoralis was distributed in relatively unstable habitat of sand dunes due to the large amount of sand movement. V. rotundifolia was distributed in a relatively stable habitat. Factors that have the greatest influence on distribution of halophytes in the Goraebul sand dunes are distance from the seashore, topography, and the pine forest. The Goraebul sand dune is a relatively well-preserved area with minimal human intervention. Therefore, different distribution of physico-chemical factors by natural processes is essential to spatial distribution of halophytes than other sand dunes in Korea. Significant natural processes in the Goraebul sand dunes were advance and retreat of coastlines from waves, erosion and sedimentation of sand due to wind and waves, and dispersal of seawater.

자궁내막암 56예의 임상병리적 특성 및예후에 관한 고찰

정민형,이선경,김승보 대한산부인과학회 2002 Obstetrics & Gynecology Science Vol.45 No.4

목적 : 본 연구는 자궁내막암의 치료 및 예후에 영향을 주는 임상병리학적 특징을 알아보기 위하여 본원에서 자궁내막암으로 진단받은 45명의 환자를 대상으로 시행하였다. 연구 방법 : 1990년 1월부터 2001년 8월까지 경희의료원에서 수술적 치료후 자궁내막암으로 진단받은 45명의 환자를 대상으로 하였으며, 환자의 임상병리학적 특징과 예후인자, 질병을 확인하기 위하여 환자의 의무기록, 수술기록, 병리검사 결과지 등을 검토하였다. 환자의 생존 여부는 의무기록상 최종 추적 관찰일을 기준으로하여 마지막 추적 관찰일에서 3개월이 지난 경우에는 전화와 우편으로 생존 여부를 확인하였다. 환자의 생존율은 이를 근거로 하였으며, 환자의 임상병리학적 특징과 각 예후인자에 따른 5년 무병생존율을 조사하였으며 예후인자간의 생존율을 비교하였다. 결과 : 환자의 평균연령은 52.4세였으며, 가장 흔한 증상은 비정상 질출혈 (91.1%)이었다. 2예 (3.6%)의 유두상 장액성 암과 1예 (1.8%)의 투명세포암을 제외한 모든 환자가 자궁내막양선암 (94.6%)이었으며 6예에서 편평세포분화를 동반한 자궁내막선암의 소견을 보였다. 조직학적 분화도 (grade)는 grade 1 (53.6%), grade 2 (26.8%), grade 3 (19.6%)이었다. FIGO 수술적 병기에 따른 병기는 1기 (55.4%), 2기 (7.1%), 3기 (30.4%), 4기 (7.1%)이었다. 대부분의 환자 (87.5%)에서 수술적 처치 단독 혹은 수술적 처치후 방사선 치료를 시행하였다. 전체 환자의 5년 생존율은 65.6%이었으며, FIGO 수술적 병기 제 1기의 5년 생존율은 83.3%였고 제 2기의 5년 생존율은 66.7%, 제 3기는 37.5%, 제 4기는 33.3%였다. 재발한 경우는 단 1예에서만 보였다. 발생시 연령, 분만력, 수술 전 시행한 자궁경부 세포진 검사결과 등은 예후인자로서의 중요성을 가지지 못했다. 자궁근층 침범정도 (P<0.05), FIGO 수술적 병기 (P<0.05), 복강내 세포진 검사 (P<0.05), 림프절 전이 유무 (P<0.05) 등이 단변량 분석상 통계적으로 유의한 결과를 보였다. 결론 : 대상 환자의 수가 충분하지는 않았지만 자궁근층의 침범정도, FIGO 수술적 병기, 림프절 전이 유무 등이 자궁 내막암의 예후인자로서의 중요성을 가지는 것으로 조사되었다. Objectives : This study was performed to evaluate the clinicopathologic characteristics and prognosis of uterine endometrial cancer to identify risk factors of the therapeutic and prognostic values. Methods : This retrospective study was based on medical records including operation records and pathologic reports of 56 patients who underwent surgical treatment with histologically proven endometrial cancer at Kyung-Hee University Medical Center from Sep. 1979 to Aug. 2001. The survival of patients was determined by description of last follow up date in medical records. If more than 3 months passed from last follow up date, we used mail and phone call to identify the status of patients. Univariate analysis was carried out to compare the importance of prognostic variables. Results : The mean age of these patients was 52.4 years, the most common presenting symptom was abnormal vaginal bleeding (91.1%). All except 2 cases of papillary serous carcinoma (3.6%) and 1 case of clear cell carcinoma (1.8%) were endometrioid adenocarcinoma (94.6%). And there are 6 cases of endometrial carcinoma with squamous differentiation. The grades were classified into grade 1 (53.6%), grade 2 (26.8%), grade 3 (19.6%). The FIGO surgical stages were classified into stageⅠ (55.4%), stage Ⅱ (7.1%), stage Ⅲ (30.4%), stage Ⅳ (7.1%). Most of the patients (87.5%) were treated by surgery only or surgery followed by adjuvant radiotherapy. The overall 5 years survival rate (5YSR) was 65.6%: The FIGO surgical stage Ⅰ 83.3%; stage Ⅱ 66.7%; stage Ⅲ 37.5%; stage Ⅳ 33.3%. Recurrent case was only one case. The age, parity, and preoperative Pap test were not significant prognostic factors. The depth of myometrium invasion (p<0.05), FIGO surgical stage (p<0.05), peritoneal cytology (p<0.05) and lymph node metastasis (p<0.05) were significant prognostic factors by univariate analysis. Conclusions : Although the number of patients was not enough, the depth of myometrial invasion, FIGO surgical stage and lymph node metastasis seemed to be significant prognostic factors of uterine endometrial cancer.

Population dynamics of mosquitoes (Diptera: Culicidae) and their disease-harboring rates in Gyeonggi province, South Korea

정민형,Kim Soowan,이두형 한국응용곤충학회 2021 Journal of Asia-Pacific Entomology Vol.24 No.2

Population dynamics and species composition of mosquitoes were monitored in a suburban residential area and rural areas, including a migratory bird refuge area and a cattle farm area, from 2017 to 2019. Mosquitoes were collected every two weeks using one BG sentinel trap and two black-light traps in a suburban residential area and a migratory bird refuge area, and one black-light trap in a cattle farm area from March to November. From the surveillance, a total of 13,267, 9,697 and 6,688 mosquitoes were collected in 2017, 2018, and 2019, respectively. The peak of the mosquito population was observed in August and September in both the suburban residential area and rural areas. In general, Culex pipiens pallens was the dominant species in the suburban residential area, whereas Aedes vexans nipponii and Anopheles spp. were the most dominant in the rural areas. Collected mosquitoes were pooled into groups for each collection site and species to investigate flavivirus harboring. Three pools were positive for Japanese encephalitis virus type V, one pool was positive for Chaoyang virus, and six pools were positive for Culex flavi virus from the 731 pools tested. When total numbers of collected mosquitoes were further analyzed by linear regression analysis for their interaction with ambient temperature and relative humidity, the number of mosquitoes were significantly affected by the ambient temperature. In contrast, relative humidity was significantly affecting the total number of mosquitoes only for the migratory bird refuge area.

부신 선종에 의한 쿠싱증후군에 동반된 척추압박골절

정민형,최현,이태화,이보연,김지열,김흥열 대한골다공증학회 2009 Osteoporosis and Sarcopenia Vol.7 No.3

본 증례는 무월경, 요추부 통증, 하지 부종을 호소하는 32세 여성에서 증상의 발생, 진단 및 치료에 대한 것으로 환자는 2번 요추의 압박골절, 원인불명의 양하지 부종, 심한 골다공증을 보였다. 환자는 골다공증의 원인에 대해 여러 전문 진료과에 협진 의뢰 후 부신 선종에 의한 쿠싱증후군으로 진단되었다. 부신절제술, 물리치료, 약물 치료를 통해 골다공증의 진행을 예방하였다.

마이크로시멘트 무기질계 그라우팅 ENG의 적용성 연구

정민형,김용식,정춘학,이송 한국구조물진단유지관리공학회 2010 한국구조물진단유지관리공학회 논문집 Vol.14 No.1

물유리계 그라우팅 공법은 침투주입 그라우팅으로 자주 적용되고 있으나, 시간경과에 따른 내구성 저하와 환경에 부정적 영향에 대한 문제가 부각되고 있다. 이에 마이크로시멘트계 무기질 재료의 ENG 공법이 이러한 물유리계 그라우팅 공법의 문제점을 극복하면서 우수한 침투성을 목표로 하여 개발되었다. ENG의 적용성을 파악하기 위해 체적변형시험, 용탈실험, 일축압축실험, 실내 투수실험 및 현장투수실험 그리고 중금속검출실험을 실시하였다. 그 결과 체적변형, 용탈량, 일축압축강도는 물유리 공법보다 월등히 우수하였으며, 투수실험에 있어서도 차수효과가 나타났다. 또한 중금속 검출량도 미미하여 환경에 부정적인 효과도 적을 것으로 판단된다. The Water glass grouting method has been applied frequently to penetration grouting in practice, but some problems, such as decrease of durability with the elapsed time and environmentally adverse effect, are raised recently. Hence, the Earth Natural Grouting method which uses micro cement and inorganic material is developed to overcomes those problems of the water glass grouting method, and is aimed for extensive ground injection bound. Volumetric strain test, syneresis test, unconfined compression test, triaxial permeability test, in-situ permeability test and heavy metal analysis were conducted to verify application of the ENG. As the result of tests, volumetric strain, syneresis and unconfined strength of the ENG were superior to those of the Water Glass SGR and ENG was proved to be impermeable. Also it is expected that the ENG would not have an effect on environmental pollution.

Effect of gut symbiont Caballeronia insecticola on life history and behavioral traits of male host Riptortus pedestris (Hemiptera: Alydidae)

정민형,이두형 한국응용곤충학회 2023 Journal of Asia-Pacific Entomology Vol.26 No.2

Symbiotic microorganisms can affect physiological conditions of host insects, subsequently altering life history and behavioral traits of their host insects. The bean bug, Riptortus pedestris, is a polyphagous insect pest which established an insect-microbe symbiosis with the bacterial genus Caballeronia. To understand how Caballeronia bacteria could affect life history and behavioral traits of host R. pedestris, we evaluated morphometric charac teristics, feeding behavior, dispersal behavior, and survivorship of symbiotic and apo-symbiotic adult male R. pedestris. From morphometric character evaluation, symbiotic male displayed significantly greater body weight and larger body size than apo-symbiotic males. Moreover, symbiotic males had significantly larger hind legs utilized as a weapon in male-male competition for R. pedestris. From feeding behavior evaluation, symbiotic male consumed greater amount of food with lesser feeding attempt, resulting in significantly enhanced feeding efficiency. Dispersal behavior evaluation revealed that symbiotic male displayed significantly enhanced move ment and flight capacity compared to apo-symbiotic male. In particular, flight capacity of symbiotic male increased ca. 170 times compared to apo-symbiotic males, yielding 1.39 km flight distance an average. There was no significant difference in survivorship between symbiotic and apo-symbiotic male R. pedestris, yielding 97 % and 90 % of survivorship over 60 days evaluation, respectively. However, titer of Caballeronia symbiont in male R. pedestris gradually decreased during the adult period evaluated. Our results demonstrated how gut microbe could affect the fitness-related and dispersal ability of male host insect.