Colorectal Cancer Therapy Using a Pediococcus pentosaceus SL4 Drug Delivery System Secreting Lactic Acid Bacteria-Derived Protein p8

정명준,안병철,Yongku Ryu,Yeo-Sang Yoon,Oksik Choi,박호진,김태엽,김송인,김봉규 한국분자세포생물학회 2019 Molecules and cells Vol.42 No.11

Despite decades of research into colorectal cancer (CRC), there is an ongoing need for treatments that are more effective and safer than those currently available. Lactic acid bacteria (LAB) show beneficial effects in the context of several diseases, including CRC, and are generally regarded as safe. Here, we isolated a Lactobacillus rhamnosus (LR)-derived therapeutic protein, p8, which suppressed CRC proliferation. We found that p8 translocated specifically to the cytosol of DLD-1 cells. Moreover, p8 down-regulated expression of Cyclin B1 and Cdk1, both of which are required for cell cycle progression. We confirmed that p8 exerted strong anti-proliferative activity in a mouse CRC xenograft model. Intraperitoneal injection of recombinant p8 (r-p8) led to a significant reduction (up to 59%) in tumor mass when compared with controls. In recent years, bacterial drug delivery systems (DDSs) have proven to be effective therapeutic agents for acute colitis. Therefore, we aimed to use such systems, particularly LAB, to generate the valuable therapeutic proteins to treat CRC. To this end, we developed a gene expression cassette capable of inducing secretion of large amounts of p8 protein from Pediococcus pentosaceus SL4 (PP). We then confirmed that this protein (PP-p8) exerted anti-proliferative activity in a mouse CRC xenograft model. Oral administration of PP-p8 DDS led to a marked reduction in tumor mass (up to 64%) compared with controls. The PP-p8 DDS using LAB described herein has advantages over other therapeutics; these advantages include improved safety (the protein is a probiotic), cost-free purification, and specific targeting of CRC cells.

딥러닝을 이용한 기계부품 자동피킹 시스템

이승현(S.H. Lee),이영재(Y.J. Lee),김동환(D.H. Kim) 한국생산제조학회 2020 한국생산제조시스템학회 학술발표대회 논문집 Vol.2020 No.12

Sn-3.5Ag/Cu의 계면반응 및 접합특성

정명준,이경구,이도재,Jung, Myoung-Joon,Lee, Kyung-Ku,Lee, Doh-Jae 한국재료학회 1999 한국재료학회지 Vol.9 No.7

The interfacial reaction and joint properties of Sn-3.5Ag/Cu and Sn-3.5Ag-1Zn/Cu joint were studied. Modified double lap shear solder joints of Sn-3.5Ag and Sn-3.5Ag- lZn solder were aged for 60days at $100^{\circ}C$ and $150^{\circ}C$ and then loaded to failure in shear. The Sn-3.5Ag/Cu had a fast growth rate of the reaction layer in comparison with the Sn-3.5Ag-lZn at the aging temperature of $150^{\circ}C$ Through the SEM/EDS analysis of solder joint, it was proved that intermatallic layer was $Cu_{6}Sn_5$ phase and aged specimens showed that intermatallic layer grew in proportion to $t_{1/2}$, and the precipitate of $Ag_3Sn$ occur to both inner layer and interface of layer and solder. In case of Zn-containing composite solder, $Cu_{6}Sn_{5}$ phase formed at the side of substrate and $Cu_{5}Zn_{8}$ phase formed at the other side in double layer. The shear strength of the Sn-3.5Ag/Cu joint improved by addition of IZn. The strength of the joint increases with strain rate and decreases with aging temperature Sn-3.5Ag, Sn-3.5Ag-lZn Eoa납과 Cu기판과의 계면반응 및 접합특성에 관하여 검토하였다. Eoa납과 Cu기판이 접합된 시편은 $100^{\circ}C$와 $160^{\circ}C$에서 60일간 열처리하였으며, 전단하중을 가하여 강도를 측정하였다. $150^{\circ}C$에서 열처리에 따른 계면반응층의 두게는 Sn-3.5Ag/Cu계면이 Sn-3.5A9-IZn/Cu계면보다 빠르게 성장하였으며, 반응생성물 성장은$ t_{1/2}$에 비례하여 체적 확산 경향을 나타냈다. 계면 반응생성물은 Sn-3.5Ag/Cu계면의 경우 $Cu_{6}Sn_{5}$상이 형성되었고, $Ag_3Sn$상은 반응층 내부 및 반응층과 땜납의 계면에 석출하였으며, Zn을 첨가한 경우에는 계면에 $Cu_{6}Sn_{5}$ 상과 함께 $Cu_{5}Zn_{8}$상이 형성되었다. 땜납/기판의 전단강도는 Sn-3.5Ag합금에 Zn을 1% 첨가하면 증가하였으며, 열처리를 한 경우에는 감소하였다.

로터리형 원자층 증착법을 이용한 Bi-Te계 소결체의 열전 성능 개선

정명준,박지영,은수민,최병준 한국분말재료학회 2023 한국분말재료학회지 (KPMI) Vol.30 No.2

Thermoelectric materials and devices are energy-harvesting devices that can effectively recycle waste heat into electricity. Thermoelectric power generation is widely used in factories, engines, and even in human bodies as they continuously generate heat. However, thermoelectric elements exhibit poor performance and low energy efficiency; research is being conducted to find new materials or improve the thermoelectric performance of existing materials, that is, by ensuring a high figure-of-merit (zT) value. For increasing zT, higher σ (electrical conductivity) and S (Seebeck coefficient) and a lower к (thermal conductivity) are required. Here, interface engineering by atomic layer deposition (ALD) is used to increase zT of n-type BiTeSe (BTS) thermoelectric powders. ALD of the BTS powders is performed in a rotary-type ALD reactor, and 40 to 100 ALD cycles of ZnO thin films are conducted at 100oC. The physical and chemical properties and thermoelectric performance of the ALD-coated BTS powders and pellets are characterized. It is revealed that electrical conductivity and thermal conductivity are decoupled, and thus, zT of ALD-coated BTS pellets is increased by more than 60% compared to that of the uncoated BTS pellets. This result can be utilized in a novel method for improving the thermoelectric efficiency in materials processing.

대안대학운동의 현황과 미래

정명준 뉴 래디컬 리뷰 2018 뉴 래디컬 리뷰 Vol.- No.76

종이 보존성 평가를 위한 수용성 물질의 활용 가능성

정명준,황유진,강규영,양봉숙,( Antje Potthast ) 한국공업화학회 2022 한국공업화학회 연구논문 초록집 Vol.2022 No.1

다결정 SnSe 열전 재료의 성능 개선 연구 동향

정명준,최병준 한국분말재료학회 2022 한국분말재료학회지 (KPMI) Vol.29 No.2

Thermoelectric materials can reversely convert heat and electricity into each other; therefore, they can be very useful for energy harvesting from heat waste. Among many thermoelectrical materials, SnSe exhibits outstanding thermoelectric performance along the particular direction of a single crystal. However, single-crystal SnSe has poor mechanical properties and thus it is difficult to apply for mass production. Therefore, polycrystalline SnSe materials may be used to replace single-crystal SnSe by overcoming its inferior thermoelectric performance owing to surface oxidation. Considerable efforts are currently focused on enhancing the thermoelectric performance of polycrystalline SnSe. In this study, we briefly review various enhancement methods for SnSe thermoelectric materials, including doping, texturing, and nano-structuring. Finally, we discuss the future prospects of SnSe thermoelectric powder materials.

대역 분할 처리를 통한 데몬 처리 성능 향상 기법

정명준,황수복,이승우,김진석,Cheong, Myoung Jun,Hwang, Soo Bok,Lee, Seung Woo,Kim, Jin Seok 한국음향학회 2013 韓國音響學會誌 Vol.32 No.2

수동 소나는 데몬 처리를 통해 수중 표적의 방사 소음으로부터 프로펠러 정보를 분석한다. 기존 데몬 처리 기법은 신호대 잡음비(Signal to Noise Ratio)를 높이기 위해 시간 영역에 대해 음향 신호를 분할하여 중첩 처리하는 방법을 사용하였다. 다시 말해 일정 시간동안 음향 신호를 수집 및 분할 처리 후 평균을 취해 잡음의 분산(variance)을 감소시켜 신호대 잡음비를 향상시켰다. 그러나 이러한 방법은 각 처리 구간의 음향 신호가 서로 독립적이지 않아 높은 성능 향상을 위해서는 많은 시간과 연산량이 필요하다. 따라서 본 논문에서는 대역 분할 처리를 통한 데몬 처리 성능 향상 기법을 제안한다. 제안된 기법은 주파수 영역에서 음향 신호의 구간을 분할하여 데몬 처리를 수행하므로 기존 중첩 기법에 비해 짧은 시간안에 서로 독립적 음향 신호 수집이 가능하다. 따라서 기존 기법에 비해 적은 시간과 연산량으로 동일한 성능을 발휘할 수 있다. 제안된 기법은 수학적 분석 및 시뮬레이션을 통해 기존 기법에 비해 성능이 우수함을 검증하였다. Passive sonars employ DEMON (Detection of Envelope Modulation on Noise) processing to extract propeller information from the radiated noise of underwater targets. Conventional DEMON processing improves SNR(Signal to Noise Ratio) characteristic by Welch method. The conventional Welch method overlaps several different time domain DEMON outputs to reduce the variance. However, the conventional methods have high computational complexity to get high SNR with correlated acoustic signals. In this paper, we propose new DEMON processing method that divides acoustic signal into several frequency bands before DEMON processing and averages each DEMON outputs. Therefore, the proposed method gathers independent acoustic signal faster than conventional method with low computational complexity. We prove the performance of the proposed method with mathematical analysis and computer simulations.

회전형 원자층 증착기의 회전 속도에 따른 SnSe 분말 상 ZnO 박막 증착

정명준,윤예준,변종민,최병준,Jung, Myeong Jun,Yun, Ye Jun,Byun, Jongmin,Choi, Byung Joon 한국분말재료학회 (*구 분말야금학회) 2021 한국분말재료학회지 (KPMI) Vol.28 No.3

The SnSe single crystal shows an outstanding figure of merit (ZT) of 2.6 at 973 K; thus, it is considered to be a promising thermoelectric material. However, the mass production of SnSe single crystals is difficult, and their mechanical properties are poor. Alternatively, we can use polycrystalline SnSe powder, which has better mechanical properties. In this study, surface modification by atomic layer deposition (ALD) is chosen to increase the ZT value of SnSe polycrystalline powder. SnSe powder is ground by a ball mill. An ALD coating process using a rotary-type reactor is adopted. ZnO thin films are grown by 100 ALD cycles using diethylzinc and H₂O as precursors at 100℃. ALD is performed at rotation speeds of 30, 40, 50, and 60 rpm to examine the effects of rotation speed on the thin film characteristics. The physical and chemical properties of ALD-coated SnSe powders are characterized by scanning and tunneling electron microscopy combined with energy-dispersive spectroscopy. The results reveal that a smooth oxygen-rich ZnO layer is grown on SnSe at a rotation speed of 30 rpm. This result can be applied for the uniform coating of a ZnO layer on various powder materials.

제지용 섬유의 Carboxymethyl화 및 Carbamoylethyl화에 의한 sheet의 습윤강도 향상

정명준,권오철,조병묵,오정수 동국대학교 생명자원과학대학 연습림 2000 연습림논문집 Vol.- No.6

Paper has been an essential part of our civilization for at least two thousand years and we can get various functional paper given by the treatment of coating, laminating, blending and addition of chemicals. Above all, wet strength paper is an important group of other grade paper. Certain paper require a wet strength in order to maintain their properties under wet or humidity conditions. For the purpose of improving the wet strength properties of paper, cellulose fiber was modified by the process of carbamoylethylation and carboxy- methylation. We have the carbamoylethylation of cellulose fiber reacted with acryl- amide in alkali catalyst and carboxymethylation of cellulose fiber reacted with mono- chloroacetic acid in alkali catalyst. The N-chlorocarbamoylethylated sheet and combined sheet of carboxymethylated and carbamoylethylated cellulose fiber more effect than untreated sheet in dry strength. As for folding endurance, the combination of carboxymethylated cellulose fiber and carbamoylethylated cellulose fiber sheet were more effective than other modified fibers. The combination of carboxymethylated cellulose fiber and carbamoylethylated cellulose fiber sheet has some effect on wet tensile strength, but this sheet it less effective than N-chlorocarbamoylethylated sheet.