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Bacillus sp . K - 1 과 그의 변이주가 생산하는 Biopolymer 의 물성에 관한 연구
정낙현(Nack Hyun Choung),김성호(Sung Ho Kim),임무현(Moo Hyun Yim) 한국응용생명화학회 1996 Applied Biological Chemistry (Appl Biol Chem) Vol.39 No.3
The rheological properties of biopolymers produced by Bacillus sp. K-1 and its mutant strains(KM-21, KM-83) were studied. Apparent viscosity of biopolymers decreased as rising the temperature. The biopolymer produced by KM-21 strain showed 1.7 times low viscosity, 2.7 times low viscosity by K-1 and 1.9 times low viscosity try KM-83 at 80℃ compared with at 20℃ respectively. The viscosity of biopolymer solution also increased with increasing the polymer concentration and showed pseudoplastic characteristics which is high temperature dependency in all concentration. The change of the biopolymer viscosity on the pH showed the highest value at the pH 7.0 and it showed lower at acidic condition than at alkaline condition comparatively.
Bacillus sp. K-1과 그의 변이주가 생산하는 Biopolymer의 물성에 관한 연구
김성호,임무현,정낙현,Kim, Sung-Ho,Yim, Moo-Hyun,Choung, Nack-Hyun 한국응용생명화학회 1996 Applied Biological Chemistry (Appl Biol Chem) Vol.44 No.1
볏짚으로부터 분리한 Bacillus sp. K-1과 그 변이주 KM-21과 KM-83이 생산하는 biopolymer들의 물성학적 성질을 비교 조사하였다. 은도에 따른 biopolymer의 겉보기 점도는 온도가 높아짐에 따라 감소하였으며, $20^{\circ}C$일 때에 비해 $80^{\circ}C$일 때 KM-21 균주가 생산하는 biopolymer는 1.7배, K-1균주의 biopolymer는 2.7배, KM-83 균주의 biopolymer는 1.9배 정도로 낮은 점성을 보였다. Biopolymer 용액의 점성은 농도가 증가함에 따라 크게 증가하여 6%의 농도에서 가장 높게 나타났으며, 2%, 4%, 6% 모두 pseudoplastic특성을 나타내어 농도에 대한 의존성이 매우 높았다. 6%의 농도일 때 2%의 농도와 비교해서 전단 속도가 $93\;sec^{-1}$ 일 때 KM-21은 26배, K-1은 56배, KM-83은 28배로 모균주의 biopolymer인 K-1이 가장 높은 점성을 보었다. pH에 따른 점도 변화는 pH7.0에서 최고를 보였으며, 알칼리성 조건일 때에 비해 산성조건에서 상대적으로 낮은 값의 점성을 보였다. The rheological properties of biopolymers produced by Bacillus sp. K-1 and its mutant strains(KM-21, KM-83) were studied. Apparent viscosity of biopolymers decreased as rising the temperature. The biopolymer produced by KM-21 strain showed 1.7 times low viscosity, 2.7 times low viscosity by K-1 and 1.9 times low viscosity by KM-83 at $80^{\circ}C$ compared with at $20^{\circ}C$ respectively. The viscosity of biopolymer solution also increased with increasing the polymer concentration and showed pseudoplastic characteristics which is high temperature dependency in all concentration. The change of the biopolymer viscosity on the pH showed the highest value at the pH 7.0 and it showed lower at acidic condition than at alkaline condition comparatively.
이후섭,정낙현,임무현 대구대학교 기초과학연구소 1987 基礎科學硏究 Vol.4 No.-
In order to develop the quality and shelf-life of the low salt-fermented Clupanodon osdeckii, some additives and preservatives such as lactic acid, sorbitol and ethanol were added along with 8% salt. The results were summerized as follows: 1. The changes on chemical composition were negligible during the fermentation. 2. The changes of microflora was that the lactic bacteria conducted the most superier act at the aerobic bacteria showed steady growth through the fermentation and the yeast growth was observed in middle period of fermentation. 3. pH values gradually declined to 6. 0, 5. 8 for the high salt-fermented Clupanodon osdeckii and the high salt-fermented entrails of Clupanodon osdeckii respectively while 5.2, 4.8 for the low salt-fermented Clupanodon osdeckii and the low salt-fermented entrails of Clupanodon osdeckii respectively at 40 days aging, however, pH values became steady thereafter. On the other hand the acidity reached to 1650cng% in high salt-fermented Clupanodon osdeckii, 2440mg% in low salt-fermented Clupanodon osdeckii, 1880mg;o in high salt-fermented entrails of Clupanodon osdeckii and 2580mg% in low salt-fermented entrails of Clupanodon osdeckii at 40 days fermentation. 4. The NIz-N content showed the maximum value of 3980mg%, 5050mg% in the high salt-fermented Clupanodon osdeckii and the high salt-fermented entrails of Clupanodon osdeckii at 60 days, 4850mg%, 5980mg% in the low salt-fermented Clupanodon osdeckii and low salt-fermented entrails of Clupanodon osdechii at 40 days respectively. 5. Both of protease and lipase activities showed the maximum unit at 60 days of high salted fermentation and at 40 days of low salted fermentation. 6. Low salt-fermented products showed almost equal quality in shelf-life and rather showed superior results in organoleptic test than that of high salt-fermented products.
Bacillus subtilis K-1에 의한 biopolymer의 생산과 levansucrase활성의 최적배양조건
김성호,정낙현,임무현 大邱大學校 科學技術硏究所 1996 科學技術硏究 Vol.3 No.1
In order to study on the biopolymer production and the changes of its molecular composition, optimal culture condition for biopolymer production, levansucrase activity increase using identified strain and molecular structure and conformation of produced biopolymer were investigated. The mutants which was improved production ability of biopolymer and changed levansucrase activity were derived. The optimal culture conditions for bioplymer production and levansucrase activity increase were sucrose 6%, M.S.G. 2%, NaH₂P0₄·2H₂O 0.3%, MgSO₄·7H₂O 0.05%, biotin 0.1㎍/㎖ at pH 6.0, 37℃ for 48hours. Under this conditions, biopolymer production and levansucrase activity were about 25g/ℓ, 830 unit respectively. Among the several mutants which were gaind by UV light irradition, levansucrase activity of mutant M-4 was 3 fold higher and biopolymer production of mutant M-1 was 2 fold higher than wild type strain..
임무현,정낙현 대구대학교 산업기술연구소 1989 産業技術硏究 Vol.8 No.-
Inspite of numorous challenges to produce a new microbial polymer which has similar properties, characteristics and effectiveness for the industry such as natural polymer. There are few of microbial polymer produced as industrial scale and efficiently enough to create volume markets. This article will concentrate largely on the production of poly-β-hydroxybutyrate where some of the most advanced technology has been applied. If the mass production methods and improving technics of properties of PHB will be proceeded, the future for PHB looks exciting provided that they cal fulfil requirements of price and performance.