Atractylis속(국화과)의 화분형태

정규영(Gyu-Young Chung),정형진(Hyung-Jin Jeong),김미숙(Mi-Suk Kim),윤창영(Chang-Young Yoon) 한국자원식물학회 2003 한국자원식물학회지 Vol.16 No.1

본 연구에서는 Atractylodes속과 혼용되는 Atractylis속 6분류군의 화분을 광학현미경과 주사전자현미경으로 관찰하였다 본 속의 화분은 단립(monad)으로, 극축의 길이는 46.5-66.7㎛, 적도면의 나비는 43.6-60.47㎛이 었다. 발아구는 3공구형 (tri-colporate)으로서 , 구구(colpus)의 길이는 19.6-29.1 ㎛, 나비는 7.3-11.0㎛이 었고, 공구의 직경 은 6.4-10.6㎛ 이었다. 화분벽은 외표벽 (ektexine)은 기저층(foot layer)과 원주층(columellae), 피복층(tectum)으로 구성되고, 두에는 5.2-8.3㎛이었다. 화분의 표면 돌기는 자상(echinate)으로서 길이가 1.4-5.8㎛이었으며, 단위면적당(No./20㎛²) 분포는 6-33개 이었다 본 연구에서 취급된 분류군들의 화분, 특히 Atractylis arabica, A. aristata, A. carduus, A. microcephala의 화분형 태는 Atractylodes속의 화분과 매우 유사하였으며, 따라서 화분에 관련된 형질은 두 속을 구분하는 식별형질로 적용할 수 없었다. 그러나 화분에 관련된 형질이 Atractylodes속에서는 화분의 크기만이 속내 분류군간의 식별형질로서 기여도가 적은 반면, Atractylis속에서는 화분의 크기뿐만 아니라 형태 및 표면무늬와 자상돌기의 크기 등에 있어서 분류군간 의 식별형질로서 적용되어 분류학적 기여도가 다소 큰 것으로 판단된다. 또한 본 연구에서 취급된 분류군 중 A. cancellata와 A. prolifera는 나머지 분류군들과 달리 자상돌기가 작고 적도면상이 긴 특징은 본속의 화분의 진화경 향성을 파악하는데 매우 유용하였다. Pollens of six taxa in the genus Atractytis L. confused with Atractylodes DC. were investigated by the light and the scanning electron microscope. Pollen grains of six taxa in this genus were monads, 46.5-66.7㎛ in length of polar axis, 43.6-60.47㎛ in equatorial width. Aperture was tri-colporate, 19.6-29.1 ㎛ in colpus length,7.3-11.0㎛ in colpus width and 6.4-10.6㎛ in porus diameter. The exine was composed of three layers(foot layer, columellne, tectum), and 5.2-8.3 ㎛ in thickness. The surface sculpturing pattern was echinate, the spines were 1.4-5.8㎛ in length and 6-33 per 20㎛² in distribution numbers over the surface. Atractylis L. was not distinguished from Atractylodes DC. by pollen morphology, especially the pollens of Atractylis arabica, A. aristata, A. carduus, A. microcephala were very similar to that of Atractylodes. But its taxonomic significance was different, the taxa of Atractylodes DC. were divided into 2 types by only pollen size, but the treated taxa in this study were divided into various types by pollen size, shape of equatorial view, surface sculpturing and spine size. The characteristics of spine size and shape of equatorial view in A. cancellata and A. prolifera were very useful for consideration of evolutionary trends in this genus.

한국산 박쥐나물속(국화과)의 외부형태와 체세포 염색체수에 의한 분류학적 연구

정규영(Gyu Young Chung),남기흠(Gi-Hum Nam),박명순(Myung Soon Park),정형진(Hyung Jin Jeong) 한국자원식물학회 2006 한국자원식물학회지 Vol.19 No.2

The morphology and somatic chromosome numbers of Korean Parasenecio were investigated to estimate their taxonomic values. Some morphological characters, such as the length of whole plants, the numbers of cauline leaves, the shapes of leaf and petioles, the numbers of involucral bracts, floret numbers per head, presence of trichomes on the style, ratio between wide part and narrow part of floret and the size of achene were found to be a good characters for delimiting each taxa. Based upon these characters, six taxa, Parasenecio auriculala var. auriculata, P. auriculata var. kamtschatica, P. hastata subsp. orientalis, P. koraiensis, P. firmus, P. pseudotaimingasa, were recognized about their distribution in Korea, but 2 taxa, P. adenostyloides, P. auriculata var. matsumurana, were needed to be studied their taxonomic position and distribution in Korea. The somatic chromosome numbers of six taxa, P. adenostyloides, P. auriculata, P. auriculata var. kamtschatica, P. hastata subsp. orientalis, P. firmus, P. pseudotaimingasa were 2n=60 and those of P. firmus and P. pseudotaimingasa were reported first in this study. 한국산 박쥐나물속(Parasenecio)의 외부형태학적 형질 및 체세포 염색재수의 분류학적 가치를 평가하기 위하여 본 연구를 수행하였다. 외부형태학적 형질인 초장, 경생엽수, 잎의 모양, 엽병 형태, 총포편수, 소화 수, 암술대의 털 존재 유무, 소화의 가는 통부와 법은 통부의 비, 열매의 크기 등이 종을 식별하는 형질로 가치를 지니고 있었으며, 이러한 형질을 기초로 할 때, 귀박쥐나물(Parasenecio. auriculata var. auriculata), 나래 박쥐나물(P. auriculata var. kamtschatica), 민박쥐나물(P. hastata subsp. orientalis), 참나래박쥐(P. koraiensis), 병풍쌈(P. firmus), 어리병풍(P. pseudotaimingasa)의 6분류 군에 대한 국내의 분포를 확인할 수 있었으나, 게박쥐나물(P. adenastylodes)과 박쥐나물(P. auriculata var. matsumurana)의 분류학적 위치 및 국내 분포 여부에 대하여 추후 연구되어야 할 것으로 생각되었다. 게박쥐나물과 귀박쥐나물, 나래박쥐나물, 민박쥐나물, 병풍함과 어리평풍의 체세포 염색제수는 모두 2n=60이었으며, 이중 게박쥐나물과 귀박쥐나물, 나래박쥐나물, 민박쥐나물은 일본산과 중국산의 염색계수와 일치하였으며, 병풍쌈과 어리병풍의 체세포 염색체수는 본 연구에서 처음으로 밝혀졌다.

Nitric Oxide Donor가 신장 허혈 재관류 손상 시 내인성 Endothelin-1 분비에 미치는 영향

정규영(Gyu-Young Jeong),성순희(Sun Hee Sung),김상이(Sang Yee Kim),손정은(Jeong Eun Sohn),이우정(Woo Jung Lee),김유선(Yu Seun Kim),정구용(Ku-Yong Chung) 대한외과학회 2004 Annals of Surgical Treatment and Research(ASRT) Vol.66 No.3

저출력 초음파가 Microcystis sp. (Cyanobacteria)에 미치는 영향

박영미 ( Young Mi Park ),권오창 ( O Chang Kwon ),박정원 ( Jung Won Park ),정규영 ( Gyu Young Chung ),이종은 ( Jong Eun Lee ),서을원 ( Eul Won Seo ) 한국환경생물학회 2013 환경생물 : 환경생물학회지 Vol.31 No.2

본 연구는 초음파의 주파수별 노출 시간이 녹조현상을 일으키는 Microcystis sp.의 군체에 미치는 영향을 알아 보고자 하였다. 각 조사 지점 내의 녹조 생물은 남조강 (Cyanophyceae)인 Microcystis sp.가 전체 조류의 약 99.5%를 차지하였다. 초음파의 노출에 따른 녹조류의 chlorophyll-a 농도를 분석해보면 노출시간이 길어짐에 따라 점차적으로 감소하였으며, 특히 70 kHz의 주파수 영역에서 녹조 생물이 세포분열을 통한 생물량의 증가를 억제하는 데 효과적인 것으로 조사되었다. 초음파에 노출되기 전 Microcystis sp.는 단세포가 점액성 물질에 조밀하게 쌓여 있는 군체의 형태로 관찰되었으나 70 kHz 초음파에 노출된 후 Microcystis sp.의 군체의 결속이 약화되고 세포가 군체에서 떨어져 나가는 것으로 나타났다. 또한 초음파는 동·식물성 플랑크톤의 형태적 변화와 운동성에 영향을 미치지 않아 다른 수중 미생물에는 부정적인 영향을 주지 않는 것으로 확인되었다. 따라서 초음파는 Microcystis sp.의 군체와 형태적 변화를 유발하고 세포를 파괴시켜 녹조현상을 일으키는 조류의 생물량을 감소시키는 데 효과적으로 작용할 것으로 사료된다. This study aimed to investigate the effects of ultrasonic wave on colonial changes of Microcystis sp. causing water-bloom. The effects of frequency change and exposure time of ultrasonic wave on Microcystis sp. were also perceived by measuring the concentration of chlorophyll-a. The concentrations of chlorophyll-a showed a decreasing tendency in all experimental groups exposed to ultrasonic waves, especially the highest decrease at 70 kHz. Before exposed to ultrasonic wave, single cells of Microcystis sp. were observed in a form of colonies which were densely accumulated onto their mucilage layer. However, after one hour exposure to 70 kHz ultrasonic wave, the bonds of colonies were weakened and single cells were separated from the colonies. Moreover, the single cells of the colony were destroyed with the 5 hour exposure time at 70 kHz. As a result, we proposed that the frequency strength and the exposure time of ultrasonic wave might have effects in decrease of biomass of blue-green algae by reducing the concentration of chlorophyll-a, thereby changing and destroying forms of Microcystis sp. colonies.

가리봉 ( 강원 ) 의 식물상 및 보존대책

김윤식,정규영,강명숙,전의식 ( Yun Shik Kim,Gyu Young Chung,Myoung Suk Kang,Eui Shik Jeon ) 한국환경생물학회 1994 환경생물 : 환경생물학회지 Vol.12 No.2

The flora of Kari-bong (1518.5m) was investigated to compare the flora of Mt. Sorak National Park, located close by this area. The vascular plants, collected from May, 1989 to October, 1992, were consisted of a total 652 taxa; 96 families, 348 genera, 569 species, 2 subspecies, 76 varieties and 5 forms. Among them, 554 taxa (85% of total taxa) were distributed in Mt. Sorak National Park. The Korean endemic plants of this area were 37 taxa, the rare and endangered plants were 27 taxa, the special wild plants designating by Environmental Agency were 12 taxa and the alpine plants were 34 taxa. Because many plants, being necessary to conserve, are distributed, Kari-bong should be include into Mt. Sorak National Park, but if development is indespensable, destruction of natrural ecosystem must be minimized.

바위솔 추출물의 항산화활성 및 암세포 증식억제

김충현(Chung Hyun Kim),박재호(Jae Ho Park),임종국(Jong Kuk Lim),이건주(Kon Joo Lee),정규영(Gyu Young Chung),정형진(Hyung Jin Jeong) 한국약용작물학회 2003 한국약용작물학회지 Vol.11 No.1

The antioxidative activities and biological properties in the EtOAc extracts and purified extracts of Orostachys japonicus were measured by assay of DPPH, xanthine/xanthine oxidase and mammalian cells(2-12). Scavenging of DPPH radical and inhibition of xanthine/xanthine oxidase of EtOAc extracts were showed the highest activity in the arable land and in September. The S-4 fraction showed the highest activity among the silica-gel column chromatography fractions. LH-4 fraction showed higher activity than the other fractionsins in assay of DPPH and xanthine/xanthine oxidase. Fatty acids and phenolic compounds were identified by GC/MS and main compounds were 1,2,3-benzenetriol, alpha-androsta-7.14-diene in LH-4 fraction. The activities of POD and SOD in samples havested on different habitats were high such as arable land > intermountain > seashore. That of POD and SOD in crude extracts of late stage were higher than early stage. Isozyme bands of crude extracts samplinged in all habitats and all growing stage were higher than early stage. Isozyme bands of crude extracts samplinged in all habitats and all growing stages showed two bands and the signal intensity showed strongly according to passage of growing stage. The purified extracts of LH-4 fraction showed excellent inhibition effect in proliferation of HL- 60 cells and markedly suppressed colony formation in mouse fibroblast cells. Dose response between partially purified extracts(400ppm) and negative control did not produced statistically significant reduction in colony formation.

신장 허혈 재관류 손상에 미치는 Ethyl Pyruvate의 효과

정구용(Ku-Yong Chung),정규영(Gyu Young Jeong),안형준(Hyung Joon Ahn),주만기(Man Ki Ju),장혜경(Hye Kyung Chang),이우정(Woo Jung Lee),한평림(Pyung-Lim Han),김유선(Yu Seun Kim) 대한외과학회 2007 Annals of Surgical Treatment and Research(ASRT) Vol.72 No.5

Purpose: Reactive oxygen species (ROS) significantly contribute to ischemia-reperfusion injury, and are also associated with the gradual loss of renal function and renal failure following renal transplantation. Pyruvate is an endogenous antioxidant, but its use as a therapeutic agent for treating conditions mediated by oxidative stress is limited due to its poor stability in solution. However, ethyl pyruvate (EP), a soluble pyruvate derivative, has far greater stability than pyruvate; thus, may serve as a practical pyruvate precursor. Therefore, the ability of EP in the prevention of renal ischemia- reperfusion injury was assessed. Methods: Sprague-Dawley rats (n=54) were subjected to 40 minutes of renal warm ischemia. The animals were divided into three groups: the sham group without warm ischemia (n=18), the EP group (n=18, EP given before ischemia), and the ischemic control (n=18). The serum levels of creatinine and TNF-α were measured 1, 3 and 5 days after induction of ischemia. The expression of high mobility group box-1 (HMGB-1), a delayed inflammatory mediator, was also assessed by Western blot of renal specimens. Results: In the EP group, late improvements in the serum levels of creatinine and TNF-α were observed in comparison with the ischemic control. Based on this delayed effect, the expression of HMGB-1 was assessed in renal tissue. The HMGB-1 expression increased over time during the ischemia process, but EP suppressed this expression 3 and 5 days after renal ischemia-reperfusion injury. Conclusion: These results have demonstrated, for the first time, that EP ameliorates renal ischemia-reperfusion injury. EP attenuates the renal ischemia-reperfusion injury, at least in part, by suppressing the expression of HMGB-1, a late mediator of delayed inflammation.

개의 동종 부분 간이식 후 허혈

정구용(Ku-Yong Chung),성순희(Soon Hee Sung),정규영(Gyu Young Jeong),오수연(Soo Youn Oh),김유선(Yu Seun Kim),조백환(Baik-Hwan Cho),최금자(Kum Ja Choi),최용만(Yong Man Choi) 대한외과학회 2002 Annals of Surgical Treatment and Research(ASRT) Vol.63 No.5

Fusarium avenaceum에 의한 복숭아 신규 과실 썩음병 발생 보고

허아영(A Yeong Heo),구영모(Young Mo Koo),최영준(Young-Joon Choi),김상희(Sang Hee Kim),정규영(Gyu Young Chung),최형우(Hyong Woo Choi) 한국식물병리학회 2020 식물병연구 Vol.26 No.1

In July 2019, typical rot symptom was observed on peach fruits harvested from the fields at Andong, Korea. As the disease progressed, white and purple colored mycelial mat developed on the surface of the infected fruits. A causal pathogen was isolated from the infected fruit and cultured on potato dextrose agar media for identification. Fungal colonies on potato dextrose agar produced 3 pigments, including purple, yellow, and white colors. The isolate incited fruit rot symptoms on artificially inoculated peach fruits, from which the same fungus was isolated, fulfilling Koch’s postulates. Based on the morphological characteristics and sequence analysis of rDNA internal transcribed spacer, translation elongation factor 1-alpha, and β-tubulin, the causal agent of the disease was identified as Fusarium avenaceum. This study is the first report of fruit rot of peach fruits caused by Fusarium avenaceum in Korea.

생육시기 및 부위별 고추의 항산화력 및 항암 Lunasin peptide의 동정

권기수,박재호,김대섭,정진부,심영은,김미숙,이희경,정규영,정형진,Kwon Ki Soo,Park Jae Ho,Kim Dae Seop,Jeong Jin Boo,Sim Young Eun,Kim Mi Suk,Lee Hee Kyung,Chung Gyu Young,Jeong Hyung Jin 한국생명과학회 2005 생명과학회지 Vol.15 No.4

부위간의 DPPH free radical scavenging 활성은 과육은 종자 보다 높았고 생육시기간에는 후기가 초기에 비하여 높았고, Xanthine oxidase 억제활성은 종자와 과육에 비하여 매우 높았고 동정된 항 산화물은 대부분이 지방산 및 페놀성 화합물로, 과육 및 종자가 성숙 될수록 1-eicosanol, palmitic acid, linoleic acid, linolenic acid 등의 지방산 및 benzonitrile의 함량이 증가되었다. 시료 채취 시기간의 POD활성은 과육은 성숙중기에 높았으나, 종자는 성숙이 경과될수록 낮았다. SOD 활성은 과육은 생육이 경과할수록 증가하였으나, 종자는 성숙할수록 낮아졌다. 고추종자 단백질은 western blot에 의하여 lunasin이 동정되었고, 개화 후 5주부터 lunasin 생합성 되었다. 고추 종자내의 100nM lunasin 펩타이드는 종양유발 유전자가 전이된 $2\~12$ 세포의 콜로니 형성을 억제시켰으며, 결과적으로 고추 종자내에는 암 예방적 특성을 갖는 lunasin peptide가 존재한다. The non-enzymatic anti-oxidants and lunasin peptide from the extracts of the pepper were examined in order to utilize the discovery in natural products as cancer chemopreventive agents. The DPPH (1,1-diphenyl-2-picryl-hydrazyl) free radical scavenging activity on the fruit parts of the pepper was higher than that of the seed, but the difference was low. The Inhibition activity of xanthine/ xanthine oxidase in extracts of the seed was higher than that of the fruit and that of the seed on 20 days after flowering was the highest at the growing period. These were identified as fatty acids and phenolic compounds such as 1-eicosanol, palmitic acid, linoleic acid, linolenic acid and benzonitrile. The contents of fatty acids and phenolic compounds increased according to the time passing at the growing period. Peroxidase (POD) activity of the fruit at middle stage was high than that of other growing stages and that of the seed was the highest at later growing period. Though superoxide dismutase (SOD) activities in fruit were hish by passage of Slowing stage, the activity in seed was low. Lunasin was searched from seeds of the peppers by coomassie blue staining and western blot among them and we just found lunasin peptide from extracted protein of the pepper by western blot. In addition, we observed the contents of lunasin after flowering and confirmed to appear the lunasin at 35 days after flowering. We confirmed that lunasin is complex protein of maturing seeds. 100nM lunasin peptide in pepper showed inhibition effect on colony formation in $2\~12$ cells.