Effects of Glucose and IGF-I on Expression of Glucose Transporter 1 (Glut1) and Development of Preimplantation Mouse Embryo

전한식,계명찬,김종월,강춘빈,김문규 한국발생생물학회 1998 발생과 생식 Vol.2 No.2

이온 비의존적으로 작동하는 포도당 수송체 (glucose transporter 1, Glut1)는 생쥐 배아의 세포막을 경계로 포도당을 수송하는 주요통로이다. 성장인자 가운데 insulin-like growth factor-I (IGF-I)은 생쥐배아에서 포도당의 유입을 증가시키는 것으로 알려져있으나 이러한 효과가 IGF-I 의한 Glut1의 전사조절 효과에 기인한 것인지는 알려져 있지 않다. 본 연구는 포도당과 IGF-I 생쥐의 착상전 배아 발생과 G A sodium-independent facilitative glucose transporter 1 (Glut1) is a major route by which glucose can be transported across the plasma membrane of mouse embryo. Although it has been known that insulin-like growth factor-I (IGF-I) promotes glucose transport into the mouse embryo, whether IGF-I directly regulates transcription of Glut1 has been uncovered in mouse preimplantation embryo. This study was aimed to elucidate the role of glucose and IGF-I in development and Glut1 expression in preimplantation mouse embryo. Two-cell embryos developed in blastocyst regardless of the glucose in the presence of pyruvate. IGF-I significantly increased the number of blastomeres in the mid-blastula. Deprivation of glucose did not affect the amount of Glut1 transcripts in morula cultured from 2-cell embryo. IGF-I potentiated Glut1 expression in morula cultured from 2-cell embryo even in the absence of glucose. Taken together, it is concluded that depletion of glucose does not promote Glut1 expression the in morula cultured form 2-cell embryo, and that increment of Glut1 expression possibly mediates embryotropic effect of IGF-I on preimplantation mouse embryo.

시설참외 연작재배지 토양의 객토 및 심토반전이 토양 화학성 변화와 토양선충 억제효과에 미치는 영향

전한식,박우철,정재식 한국환경농학회 2002 한국환경농학회지 Vol.21 No.1

대형증발계 정밀 관측에 관한 연구

전한식,이부용 한국기상학회 2010 한국기상학회 학술대회 논문집 Vol.2010 No.10

시설참외 연작재배지 토양의 객토 및 심토반전이 토양 화학성 변화와 토양선층 억제효과에 미치는 영향

전한식,박우철,정재식 한국환경농학회 2002 한국환경농학회지 Vol.21 No.1

우리나라의 시설참외 수산지인 성주지역의 시설참외 연작 대책으로 객토와 심경에 의한 토양 화학성분의 변화와 뿌리혹선충 억제 효과를 조사한 결과는 다음과 같다. 시설참외 연작대책으로 많은 양의 객토를 함으로 EC는 4.30 dS/m에서 1.80 dS/m로 58%, 유효인산은 406mg/kg으로 55% 감축되고 유기물 함량이 16g/kg에서 11g/kg으로 31% 줄어 퇴비 등 각종 거름 시용량을 증가해야 하면 뿌리혹선충 밀도 억제효과는 1년 차 89%, 3년 차 84%, 5년 차 69%로 되어 근본 대책이 되지 못하면서 영농비 가중 및 환경 파괴의 문제가 수반되었다. 심토반전 효과도 객토와 비슷하여 EC 4.30 dS/m에서 1.98 dS/m로 54% 줄고 유효인산 406 mg/kg에서 329 mg/kg 으로 19% 줄었으며 뿌리혹선충 밀도 억제 효과는 처리 후 1년 차 71%, 3년 차 67%, 5년 차 42%가 되므로 처리 3년 이후에는 다른 대책이 필요하였다. To elucidate the effect of soil addition and subsoil plowing on the change of soil chemical properties and the reduction of root-knot nematode, this experiment carried out in continuous cropping field of protected oriental melon (Cucumis melo L). Soil addition reduced electric conductivity (E. C.) from 4.3 to 1.8 dS/m (58%), available P_2O_5 from 406 to 182 mg/kg (55%) and organic matter content from 16 to 11 g/kg (31%). Population densities of root-knot nematode in soil reduced as much as 89%, 84%, and 69% at first year, third years, and of five years later, respectively. The effects of subsoil plowing were similar to that of soil addition. E. C. and phosphate were reduced from 4.30 to 1.98 dS/m (54%) and phosphate from 406 to 329 mg/kg (19%), respectively. Population densities of root-knot nematode reduced as much as 71%, 67%, and 42% after 1, 3, and 5 years, respectively. Subsoil plowing reduced nematode densities only for three years.

참외 連作障害 對策을 爲한 效果的인 土壤管理

全漢植(Han-Sik Chun),姜相載(Sang Jae Kang),朴愚喆(Woo Churl Park) 한국토양비료학회 1997 한국토양비료학회지 Vol.30 No.4

성주 지역 시설참외 연작지의 토양특성 및 토양선층 변화

박우철,전한식 한국환경농학회 2001 한국환경농학회지 Vol.20 No.2

시설참외 연작지 토양의 염류집적 해결과 뿌리혹선충 방제에 필요한 기초자료를 얻고자 수행한 성주 지역 시설참외지의 연작에 대한 토양 특성 및 토양선충 변화를 조사한 결과는 다음과 같다. 1. 참외 연작 년수에 따른 뿌리혹선충의 피해는 연작 3년 이하의 밭에서는 토양 300 mL당 91마리로 적어 피해가 거의 나타나지 않았으나 연작 4∼6년 차에는 선충밀도가 518마리로 증가되고 참외 고사주율도 8월에 50%로 나타나 후기 수량이 절반 이하로 떨어졌다. 2. 토양 염류집적 및 토양성분 함량은 유효인산 함량이 3년 이하 연작보다 4년 이상 연작할 때 438 ㎎/㎏로 265% 증가되었고 질소함량과 치환성 염기함량도 연작 연수가 길수록 많이 축적되었으며, 특히 염농도는 1.20에서 4.55 mS/㎝로 높아져 3년 이상 연작시에는 수량이 현저히 감소되었다. 그리고 토양유기물 함량은 9에서 16 g/㎏으로 증가되고 pH가 6.1에서 6.5로, CEC는 7.50 cmol^+/㎏에서 10.78 cmol^+/㎏로 증가하였다. 3. 경시적인 선충 감염율은 2월에는 감염주율이 15%로 낮았으나 매월 10∼20%씩 증가되었고 6월에는 45%, 7월에는 60%로 감염주율이 높았으며, 뿌리혹선충 유충 밀도는 2월 167마리이던 것이 점차 증가하여 8월에는 1,625마리로 10배의 증식율을 보였다. 4. 상토원 재료별의 선충 분리비율은 평균 35%이고 서식밀도도 300 mL당 54마리였으며, 논흙과 강변모래에서 검출 점수가 많았고 검출율은 밭흙과 논흙에 많았다. 5. 정식 본포의 토양성분 분석과 선충밀도와의 상관관계는 유효 인산, 치환상 염기는 유의성이 없었고 염농도는 사양토보다 식양토쪽으로 갈수록 3.70 mS/㎝에서 5.35 mS/㎝로 높았으며, 선충밀도는 사양토로 갈수록 토양 300 mL당 318에서 593 마리로 1.9배 증가되었다. 참외 고사주율도 8월 조사결과 식양토 50%인대 비하여 사양토 85%로 사양토에 갈수록 높았다. This study was conducted for ten years to evaluate the effective soil management for preventing the infection of root-knot nematode in the field of continuous cultivation with oriental melon under plastic house in Songju area of kyongbuk province. The content of available phosphate, total nitrogen, organic matter, CEC, and exchangeable base in the soil increased with the increase of continuous cultivation year. Especially salt content in the soil increased form 1.2 to 4.55 mS/㎝ and the yield of oriental melon dramatically decreased with the continuous cultivation year. The number of root-knot nematode was 91 per 300 ㎤ of soil in the field of continuous cultivation for 3 years and showed slight damage on the oriental melon, but it was 518 in the field of 4 ∼ 6 years continuous cultivation and showed that 50% of plants died in August, and the yield of late season was less than 50% compared to normal plant. For the seasonal changes in infection rate of root-knot nematode on oriental melon plant, 15% of the normal plant was infected by nematode in February and increased gradually by 10 ∼ 20% per month, 60% of plants was infected in July. The density of root-knot nematode nymph was 167 in February and increased to 1,625 in August. The infection rate of nematode was 35%, and the number of nematode was about 54 in nursery soil originated from paddy soil, upland soil, and river sand. There were no relationship between the number of nematode and available phosphate or exchangeable base in the soil of plastichouse where oriental melon plants were grown.

전핵 시기 및 2-4 세포 시기에 동결 보존된 배아의 발생률 및 임신률

양현원,최규완,전한식,차영범,이승재,박종민,Yang, Hyun-Won,Choi, Kyoo-Wan,Cheon, Han-Sik,Cha, Young-Beom,Lee, Seung-Jae,Park, Jong-Min 대한생식의학회 1994 Clinical and Experimental Reproductive Medicine Vol.21 No.1

The survival and pregnancy rates were compared between non-frozen embryos and cryopreserved embryos at either pronucleate or 2-4 cell stages using the freezing and thawing techniques being identical in both groups were compared with fresh embryos. 496 embryos were frozen with 1, 2-propanediol and sucrose and 117 2-4 cell stages embryos had been thawed and 79.6 and 66.0% of them respectively were survival. Clinical pregnancy rate was 19.2% for embryos frozen at the pronucleate stage and 19.0% for embryos frozen at the 2-4 cell stages while the pregnancy rate of non-frozen embryos was 21.3%. There were no significant difference in the survival and pregnancy rates of embryos frozen at pronucleate and 2-4 cell stages. The current cumulative pregnancy rate per retrieval in all cycles with frozen zygotes is 35.4 %, consid~ erably higher than observed in single transfers of embryos without cryopreservation(21.3%); predicted pregnancy rate after transfer of all frozen embryos is 43.3 %. It is concluded that firstly, the survival and pregnancy rate of cryopreserved embryos at pronucleate or 2-4 cell stages are very similar to those from their fresh embryos and non-frozen embryos and secondly, cryopreservation substantially enhances pregnancy attainment from in vitro fertilization.

일반연제 발표 : 생식내분비 및 불임 ; 투명대하 미세수정(SUZI)시 수정률과 임신률에 미치는 정자성상과 전배양액 및 배양시간의 영향

최규완,양현원,전한식,김수경,차영범,이승재,박종민,김문규 대한산부인과학회 1993 대한산부인과학회 학술대회 Vol.72 No.-

적응 요인에 따른 보조부화술 (Assited Hatching, AH)의 효과

김지수,강승호,권윤정,손인표,최규완,김수경,전한식,이제규,이승재,박종민,Kim, J.S.,Kang, S.H.,Kwon, Y.J.,Son, I.P.,Choi, K.W.,Kim, S.K.,Chun, H.S.,Lee, J.G.,Lee, S.J.,Park, J.M. 대한생식의학회 1998 Clinical and Experimental Reproductive Medicine Vol.25 No.2

Implantation rates remain low following human in vitro fertilization (IVF). Suboptimal culture conditions may limit the ability of embryos to hatch as blastocysts, and artificial opening of the zona pellucida has been proposed as a means to promote subsequent hatching (assisted hatching). In this study, assisted hatching (AH) by zona drilling using acidic Tyrode's solution was performed in 320 patients, due to their age of more than 38 years (group A), the thick zona pellucida (group Z; $ZP\geq0.18{\mu}m$), and failures in implantation more than 3 times in previous IVF-ET trial (group P). This study was designed firstly, to study the effects of AH on the outcomes of IVF-ET according to the indications and secondly, to verify the appropriate application of AH. The results were as follows; 1. There was no difference in pregnancy rate between AH group (26.6%) and non-AH group (26.5%). 2. Assisted hatching (AH) showed significantly higher pregnancy rate of the patients with thick zona pellucid a than those of the patients with age factor and with the history of repeated implantation failure. But in the patients with age factor only, AH resulted in higher pregnancy rate. 3. Interestingly, the patients with complex factors including zona factor (Z: 33.9%; ZA: 30.4%; ZP: 31.6%; ZAP: 21.4%) showed higher pregnancy rates than other complex factors excluding zona factor (A: 24.4%, P: 0%; AP: 10.8%). From these results, AH is more helpful to the patients with thick zona pellucida rather than patients with older age and/or previous repeated implantation failure.

경북지역 홍화재배지의 병해충 발생양상

박소득(So Deuk Park),박경석(Kyung Suk Park),김기재(Ki Jae Kim),김재철(Jae Cheol Kim),전한식(Han Sik Chun),신용습(Yong Seub Shin) 한국원예학회 2003 한국원예학회 학술발표요지 Vol.2003 No.5