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전창후,나해영 한국원예학회 2011 원예과학기술지 Vol.29 No.5
Cold pretreatment, washing medium and composition of nutrient media may have marked effects on microspore embryogenesis. When microspores isolated from radish (Raphanus sativus L. cv. Gwanhun)flower buds were washed with Nitsch & Nitsch (NLN) medium liquid medium containing 130 g·L^(-1) sucrose (NLN-13), yields of microspore-derived embryos were greater than when using B5 liquid medium containing 130 g·L^(-1) sucrose. Microspore viability is known to decrease rapidly with storage; however, in this experiment,microspore viability was maintained for 24 h at 4°C without media. Among the various medium concentrations used (0.25×, 0.5×, 1.0×, 2.0×, and 4.0× NLN liquid medium), 0.5× NLN liquid medium induced the most efficient formation of microspore-derived embryos. In addition, microspore-derived embryos yields were greater when microspores were cultured in 0.5× NLN liquid medium supplemented with 0.25×, 0.5×, and 1.0× NLN microelements, compared to medium not supplemented with microelements. In this study, the highest yield of microspore-derived embryos was observed when the microspores derived from flower buds were washed using NLN-13 liquid medium and then cultured on 0.5× NLN liquid medium supplemented with 0.25× NLN microelements, followed by incubation at 25°C for 30 days.
전창후,박한용,나해영 한국원예학회 2011 Horticulture, Environment, and Biotechnology Vol.52 No.5
Composition of nutrient media, flower bud size, sucrose concentration, heat shock stress, and ethylene inhibitor could have marked effects on microspore embryogenesis. No microspore-derived embryos (MDE) were formed when the microspores were isolated from radish (Raphanus sativus L.) flower buds of 1.0-2.5 mm in size, whereas MDE were formed with microspores isolated from 2.5-4.5 and 4.5-6.5 mm flower buds. The microspores isolated from 2.5-4.5 mm flower buds showed high embryo yields. MDE formation was highest when 150 g・L^(-1) sucrose was added to the half strength Nitsch &Nitsch (NLN) liquid medium, but at sucrose concentrations less than 100 g・L^(-1) there was no MDE formation. Microspores cultured on half strength NLN liquid medium containing 0.05 mg・L^(-1) silver nitrate (AgNO3) produced the most MDE,showing more than two-fold increase in yield compared to those cultured on medium without AgNO3. A heat shock pretreatment of microspores at 32ºC for 24 h gave high-frequency production of MDE when compare to higher or lower temperatures; no MDE were formed at 42.5ºC. The highest yield of MDE was observed when microspores were derived from 2.5-4.5 mm flower buds cultured on half strength NLN medium containing 150 g・L^(-1) sucrose, 0.05 mg・L^(-1) AgNO_3, and precultured with heat shock pretreatment of microspores at 32ºC for 24 h, followed by incubation 25ºC for 30 days. A polyploidy test indicated that 19.7% of the microspore-derived plants were doubled haploid, other plants were haploid, and chimeras were haploid and diploid.
湛液 養液栽培에 있어서 배추의 生育과 養液循環의 컴퓨터 制御
全昶厚,李炳馹 서울大學校農科大學 農業開發硏究所 1991 서울대농학연구지 Vol.16 No.1
엇갈이用 배추의 湛液水耕(deep flow technique, DFT) 栽培에 있어 養液의 溫度, 溶存酸素量 등의 環境變數를 조사하였으며, 溶存酸素量을 증가시킬 수 있는 養液의 循環方法들을 비교검토하였다. 또한 栽培環境의 計測과 養液의 循環制御에 마이크로컴퓨터 技術을 適用하였으며, 다음과 같은 結果를 얻었다. 1. 배추 生育의 最適 pH는 6.0이었다. 2. 양액의 溫度는 外部氣溫에 따라 變하였으나, bed 위의 位置에 따른 液溫의 差異는 거의 없었다. 3. 栽培의 進展에 따라서 養液의 溶存酸素量은 급격히 減少하였다. 4. 養液의 流入口로부터 멀어질수록 溶存酸素量의 減少가 심하였고, 地下部의 生育도 減少하였다. 5. 養液循環法에 있어서는 連續循環 및 1-(5)分 處理區(1分 循環, 5分 停止)에서 높은 溶存酸素 獲得效率을 얻었다. 6. 배추의 生育은 連續循環區와 1-(5) 處理區에서 우수하였고, 10-(50) 處理區와 30-(30) 處理區에서는 저조하였다. 특히, 1-(5) 處理區는 連續循環區와 거의 같은 效果를 나타내었다. 7. 連續循環區와 1-(5) 處理區에서, 10-(50) 處理區와 30-(30) 處理區에 比해 P, K, Ca, Mg 등의 植物體 無機이온 含量이 높았으며, 특히 K의 含量이 越等히 높았다. 8. 養液溫度의 digital 計測과 養液 循環方法의 制御 system을 마이크로컴퓨터 技術을 活用하여 완성하였다. In DFT (deep flow technique) of Chinese cabbage culture, the growth, temperature of nutrient fluid and dissolved oxygen concentration were investigated. For increment of dissolved oxygen concentration, the circulation methods in DFT were compared. The computer interfacing technology for the data acquisition and the environment control were successfully applied to the hydroponic system. The results are summarized as follows: 1. Optimum pH for the growth of Brassica campestris was 6.0. 2. The temperature of nutrient fluid was influenced by the ambient air temperature, but was not different at various positions on the bed. 3. The oxygen concentration of nutrient fluid was decreased faster and more remarkably. 4. Along the channel, the content of dissolved oxygen and the root growth was decreased. 5. l-(5) step (1 min. on. 5 min. off) had a great gain for oxygen concentration than any other circulation methods, controlled by on-off control. 6. The growth in continuous and 1-(5) step circulation was better than one in 30-(30) step and l0-(50) step circulation. 7. In continuous and l-(5) step circulation, the content of inorganic ions, such as P, Ca, Mg and K was more than l0-(50) step and 30-(30) step circulation. 8. Data acquisition of the temperature of nutrient fluid and flow control were accomplished with microcomputer technology.
나해영,전창후 한국원예학회 2009 원예과학기술지 Vol.27 No.2
Formation and germination of somatic embryo (SE) are affected by the concentration of plant growth regulators; MS medium strength, sucrose concentration, and the photosynthetic photon flux (PPF) level. The MS medium strength and sucrose concentration for an optimal condition of the SE formation and germination of Pimpinella brachycarpa were x0.5, sucrose 30 g L-1 and x0.25, sucrose 30 g L-1, respectively. When 0.05 mg L-1 of kinetin was treated, the percentage of normal somatic embryo (NSE) formation was the greatest and the percentage of hyperhydric somatic embryo (HSE) formation was the lowest. SE germination rate was greater in treatment of IAA 0.05 mg L-1 or BA 0.01 mg L-1 than those of other treatments. Moreover, better SE formation and germination were found at dimmer lighting (20 μmol m-2 s-1 of PPF) than that of higher PPFs. SE formation and germination were also retarded at higher PPFs. Optimum PPF for SE formation and germination was 20 μmol m-2 s-1. After forming SE from EC 0.1 g for 4 weeks, EC 0.1 g resulted in 700 total SE formation. Culturing four SEs for four weeks resulted in a secondary SE formation, germination and eventually 52 plantlets. These results indicate that a huge proliferation rate was achieved at up to 8 weeks of EC at liquid medium suspension culture and SE at solid medium culture. Through the experiment, after inducing EC from EC, the mass of EC was increased by 25 times (from 0.05 to 1.30 g) when each of the nutritional and chemical factors was controlled at the optimized condition.