PD-1 유전자 제거 마우스에서 홍역바이러스 감염

전진경,김규연,허지애,강동원,김기환,김동수,Chun, Jin Kyong,Kim, Kyu Yeun,Hur, Ji Ae,Kang, Dong Won,Kim, Ki Hwan,Kim, Dong Soo 대한소아감염학회 2013 Pediatric Infection and Vaccine Vol.20 No.3

목적: 아급성경화범뇌염은 퇴행성 신경질환으로 홍역바이러스의 지속적 중추신경계 감염으로 나타난다. 저자들은 마우스 모델을 통해 아급성경화범뇌염 발병에서 만성 바이러스 감염에 관여하는 PD-1 유전자의 역할을 알아보고자 하였다. 방법: 3주령의 동형 PD-1 유전자 제거 마우스, 이형 PD-1 유전자 제거 마우스, 야생 BALB/c 마우스를 대상으로 측뇌실내 홍역바이러스를 주입하여 동물 모델로 하였다. 바이러스 주입 3개월 후, 마우스의 뇌 조직학적 소견을 관찰하고 혈청을 분리하여 IL-21의 혈청 농도를 ELISA kit을 통해 측정하였다. 결과: 야생 BALB/c 마우스에서 가장 많은 국소적 뇌백질의 괴사 및 성상세포의 증가가 관찰되었다. 이형 마우스에서 뇌실질의 병변은 적었으며 동형 마우스는 거의 보이지 않았다. 세 그룹에서 모두 혈청 IL-21의 증가는 보이지 않았다. 결론: 이 결과는 PD-1 유전자가 만성 바이러스 감염에 중요한 역할을 함을 시사한다. Purpose: Subacute sclerosing panencephalitis (SSPE) is a neurodegerative disease due to persistent measles virus infection. We investigated the role of programmed death-1 (PD-1) molecule which is related with chronic viral infection in developing SSPE in mouse. Methods: We adopt the $PD-1^{-/-}$, $PD-1^{-/+}$, and wild type BALB/c 3 week old mice to make an animal model of SSPE by injecting measles virus (SSPE strain) intraventricularly. Three months after infusion of virus, the mice were sacrificed and examined if the typical pathologic lesions had been progressed. The sera were collected from each group of mice and the serum level of IL-21 was measured with ELISA kit. Results: The necrotic lesions on white matter and gliosis were found in focal areas in wild type BALB/c. The extent of lesion was smaller in heterotype BALB/c. Scanty lesions were found in $PD-1^{-/-}$ mice. The sera level of IL-21 was not elevated in all three groups. Conclusion: Our data suggest that the PD-1 molecule may play a role in persistent viral infection.

Analysis of Clinical Presentations of Bruton Disease: A Review of 20 Years of Accumulated Data from Pediatric Patients at Severance Hospital

전진경,이택진,송재우,인요한,김동수 연세대학교의과대학 2008 Yonsei medical journal Vol.49 No.1

Purpose: X-linked agammaglobulinemia (XLA) is a humoral immunodeficiency disease caused by a mutation in the Bruton tyrosine kinase (BTK) gene resulting in defective B cell differentiation. Because it is a relatively rare disorder, it is difficult for clinicians to have a comprehensive understanding of XLA due to a lack of exposure to the disease. Clinical presentations of patients with XLA were analyzed and discussed to improve care plans. Materials and Methods: During a 20 year period, from January 1987 to June 2006, a total of 19 patients were diagnosed as XLA in the Department of Pediatrics at Severance Hospital, Seoul, Korea. A retrospective analysis of the clinical presentations of those patients was performed. Results: The mean age of the XLA patients included in the study was 4.89 years, with a range of 6 months to 13 years. Twelve patients were diagnosed before age 5, while the other 7 patients were diagnosed after age 5. Recurrent infections observed in the patients included pneumonia, acute otitis media, septic arthritis, skin infection, sepsis, sinusitis, acute gastroenteritis, cervical lymphadenitis, epididymitis, meningitis, osteomyelitis, urinary tract infection and encephalitis. Frequency of admissions was variable from 0 to 12 times, depending on the time at which immunoglobulin therapy was started. Six cases had family histories positive for XLA. BTK gene mutations were found in 8 cases. Conclusion: The overall prognosis of XLA is good as long as patients are diagnosed and treated early with regular intra venous gamma globulin therapy before the sequelae of recurrent infections appear.

Comparison of Research Trends in Korean and International Family Medicine in Journals of Family Medicine

전진경,이정운,이동률 대한가정의학회 2014 Korean Journal of Family Medicine Vol.35 No.6

Background: Research is important for the development of family medicine as a professional field in primary care. The aim of this study was to suggest directions for the development of family medicine research by analyzing research trends in original papers published in the Korean Journal of Family Medicine (KJFM) and international journals. Methods: We investigated original research papers published in KJFM and 4 international journals from August 2009 to July 2010. Analysis was conducted according to research topics, authors, methods, participants, and data sources. Results: ‘Clinical research’ was the most common research topic in both the KJFM (88.3%) and international journals (57.3%); however, international journals had more studies in other domains (‘education and research,’ ‘health service,’ and ‘family medicine’). More authors other than family physicians participated in international journals than in the KJFM (58% and 3.3%, respectively). Most studies were ‘cross-sectional’ in KJFM (77.0%) and international journals (51.5%): however, the latter had more ‘qualitative’ studies, ‘cohort’ studies, and ‘systematic reviews’ than the former. The largest study population was ‘visitors of health promotion center’ in the KJFM and ‘outpatients’ in international journals. Most of the study sources were ‘survey’ and ‘medical records’ in both. Conclusion: There were limitations of diversity in the papers of the KJFM. Future investigation on papers of other than family medicine journals should be planned to assess research trends of family physicians.

마음수련이 스트레스와 직무만족도에 미치는 영향

전진경,박점희 경북대학교 간호과학연구소 2004 경북간호과학지 Vol.8 No.1

The purpose of this study was to examine the effect of the Maum-meditation practice on the stress and job satisfaction. Research method used in this study was descriptive research. The number of the subjects who attended the professional job training for teachers in Y-province was 97 in the pre-test, 85 in the post test respectively. The training program had been carried out from August 4 to August 9, 2003. The questionnaire survey was collected before and after the training. The instruments used for this study were 「Psychiatric Symptom Index」 by Ilfeid(1977) and 「Teacher Job Satisfaction Questionnaire(TJSQ)」by Jong hee No(2001). The data collected through the questionnaire survey were analysed to get the percentage, the mean, and the t-test score using the SAS program. The results of this study was summarized as follows. The amount of stress after the training showed a meaningful decrease compared to that of the stress before the training, marking a meaningful decrease in every area of cognitive, anxiety, depression and anger. The degree of job satisfaction after the training showed a significant increase. When analysed by areas, only three areas out of 7, such as development, renovation, and partnership showed a significant increase. The degree of the stress in relation to the general characteristics, I could find a meaningful decrease in people who are below 44 years old, in females more than males, in people who do not have any religions and who are Roman catholics. In terms of marital status, both married and single showed signifcant decrease. In terms of length of experience, people who have less than 25 years experience showed a meaningful decrease. In terms of the age of students who they teach, all the teachers except the primary teachers showed a significant decrease. In terms of the degree of job satisfaction in relation to the general characteristics, the teachers whose age ranges 35 to 44 showed a statistically meaningful increase. In terms of gender, only female teachers showed increase. In terms of religion, the atheists showed increase. In terms of marital status, the married people showed a meaningful increase. It seems, therefore, that the Maum-meditation practice is a useful intervention to decrease the stress and increase the job satisfaction of the working people. I would like to suggest that the effect of the Maum-meditation should be tested in many other professions to prove its effectiveness.

대구시, D-클라우드 구축을 통해 본 시사점

전진경 한국지역정보개발원(구 자치정보화조합) 2017 지역정보화 Vol.106 No.-

소아 잠복 결핵 감염 진단에 있어서 투베르쿨린 피부반응 검사와 결핵 특이항원 자극 Interferon-γ 분비능 측정의 비교

전진경,김창기,김현숙,정귀영,인요한,김기환,이택진,전지현,김동수,Chun, Jin-Kyong,Kim, Chang Ki,Kim, Hyun Sook,Jung, Ghee Young,Linton, John A.,Kim, Ki Hwan,Lee, Taek Jin,Jeon, Ji Hyun,Kim, Dong Soo 대한소아청소년과학회 2008 Clinical and Experimental Pediatrics (CEP) Vol.51 No.9

목 적 : 결핵 감염 관리에 있어서 잠복 결핵 감염을 찾아 내어 예방적 화학요법을 시행하는 것은 주요 핵심이다. 결핵에 노출된 소아에서 잠복 결핵 감염 진단을 위해 투베르쿨린 피부반응과 최근 널리 쓰이기 시작한 체외 검사법으로 결핵 특이항원 자극에 의한 T 세포의 $IFN-{\gamma}$ 분비능 측정 (QFT-G IT)이 있다. 잠복 결핵 감염 진단에 있어 두 검사법의 차이를 알아보고자 본 연구를 진행하였다. 방 법 : 2006년 10월 1일부터 2007년 4월 30일까지 6개월 간 총 111명의 소아를 대상으로 투베르쿨린 반응과 QFT-G IT 검사를 시행하였고, 동일 기간 내인 2개월 간 진단 된 성인 결핵 환자 29명에서 동일한 검사 결과를 후향적으로 알아보았다. 결 과 : 결핵 환자와의 가까운 접촉력을 주소로 내원한 무증상의 환자 33명 중 15% (5명)에서 QFT-G IT 검사 양성 소견을 보였고, 투베르쿨린 피부반응은 42% (14명)에서 양성결과를 보여 두 검사 간의 일치율은 낮았다(${\kappa}=0.39$). 성인 결핵 환자에서 QFT-G IT 양성율은 86.2%였고, 결핵균 배양 검사 양성율은 48.2%였다. 배양검사 양성이면서 QFT-G IT 음성이었던 환자는 12.5%였다. 결 론 : 소아에서 잠복 결핵 감염을 진단함에 있어 가족력을 근저에 둔 투베르쿨린 피부반응 검사를 시행하되 접촉력이 불분명하거나 지속적이지 않았을 경우 혹은 BCG 접종 효과로 의심되는 경우 결핵균 특이항원 자극 $IFN-{\gamma}$ 분비능 측정이 보조적 수단으로 사용 될 수 있을 지는 아직 확실하지 않으며 향후 수년 간 다 기관 연구가 필요하다. Purpose : Surveillance for detecting and managing latent tuberculosis infection (LTBI) is a key component of tuberculosis control. The classic surveillance tool, the tuberculin skin test (TST), may have some limitations when used in the Bacillus Calmette-$Gu{\acute{e}}rin$ (BCG)-vaccinated population. The object was to perform a blood test $QuantiFERON^{(R)}$-TB Gold In Tube (QFT-G IT) based on the detection of interferon-$\gamma$ ($IFN-{\gamma}$) released by T cells in response to Mycobacterium tuberculosis-specific antigens, and to compare the efficacy of this new diagnostic tool for LTBI with that of TST. Methods : For six months, between October 1, 2006 and April 30, 2007, data were collected from 111 patients under 15 years of age at Severance Children's Hospital. TST and QFT-G IT tests were performed with children with or without contact histories of tuberculosis. In addition to these tests, we examined comparative data from 29 adults who had tuberculosis, to detect false negative rates in the QFT-G IT method. Results : Thirty-three children had household contact histories. In this group, 15% and 42% of cases were found to be positive using the QFT-G IT assay and TST, respectively. Agreement was low between these two tests (${\kappa}=0.39$). In the adult active tuberculosis group, the QFT-G IT false negative rate defined as a positive culture and a negative QFT-G IT result was 12.5%. Conclusion : In diagnosing LTBI in children, the usefulness of a whole-blood $IFN-{\gamma}$ assay employing TB-specific antigens will be revealed only by examining additional longitudinal clinical data; this study serves as a starting point in that process.

The Association of Lymphopenia with the Clinical Severity in the Korean Children Admitted to the Hospital with Pandemic (H1N1) 2009 Infection

전진경,김황민,차병호,어영,김효열,김영근,권우철 대한감염학회 2011 Infection and Chemotherapy Vol.43 No.1

Background: Critical illness due to pandemic (H1N1) 2009 is an emerging threat to global health. In this study, lymphopenia was focused on as a major risk factor for a critical clinical course of pandemic (H1N1) 2009 infection. We investigated the association of lymphopenia at the time of admission with the clinical severity of the admitted children with pandemic (H1N1) 2009 infection. Material and Methods: We performed a retrospective study on the patients who were younger than 15 years of age and who were admitted to Wonju Christian Hospital due to pandemic (H1N1) 2009 infection between August 20, 2009 and February 20, 2010. Pandemic (H1N1) 2009 infection was confirmed by reverse transcriptase polymerase chain reaction (RT-PCR) in all patients. We divided the study period into two periods as August 20 -November 30 2009 (pre-vaccination period) and December 1 2009- February 20 2010 (post-vaccination period). The clinical differences between two periods were analyzed. To define the role of lymphopenia, we examined the differences of clinical manifestations between the H1N1 patients with lymphopenia and those without lymphopenia. Results: Among the 2,399 children who had H1N1 infection, 149 patients (6.2%)were admitted under the following diagnoses: pneumonia (67.1%), bronchiolitis/asthma (18.8%), croup (6%) and febrile convulsion (8.7%). The median age of the patients was significantly different between during the pre-vaccination period and the post-vaccination period (6 years of age [range: 0.25-14] vs. 3 years of age, [range:0.1-14], P<0.05). The proportion of patients who had lymphopenia was significantly different between two periods (39.5% vs. 20%, P<0.05). When we compared the clinical severity between the patients with lymphopenia and those without lymphopenia, age (P<0.0001), the length of hospital stay (P<0.0001) and the serum levels of C-reactive protein (P<0.01) were significantly different. Conclusion: Our data support that lymphopenia may be a major determining factor that could cause a critical clinical course during pandemic period among children in the Republic of Korea.

Nitric oxide-releasing methoxy PEG-PLGA nanopolymersomes to angiogenesis enhancement

양충모,전진경,서덕원,정윤,옥명렬,이강원 한국고분자학회 2017 한국고분자학회 학술대회 연구논문 초록집 Vol.42 No.1

해안도시 여고생의 학교 급식 육찬의 희망횟수 및 어찬 기호도에 관한 융합연구

박필숙,전진경,김금란,박미연 한국융합학회 2020 한국융합학회논문지 Vol.11 No.1

This study is a convergence study of 596 high school girls in order to confirm the relationship between the desired number of meat side dishes and preference of fish side dishes in school meals. Data were analyzed by χ2-test, ANOVA-test, and Duncan’s test using SPSS 22.0 program. The results of the study were as follows. The scores of the subjects for promoting palatability were highest in the items ‘Easy to eat’ and ‘Without fishy’. The scores of 'Nutrition Education', 'Combined with Preferred Food', 'Without Fishy' and 'Add Preferred Flavor' tended to increase significantly as the desired frequency of meat side dishes increased from ‘less than three times a month’ to ‘more than five times a week’. The findings of the study confirmed the relationship between the desired frequency of meat side dishes and preference of fish side dishes. Therefore, this study is expected to provide high school girls with a way to increase fish preference. 본 연구는 학교급식에서의 육찬 희망횟수와 어찬 선호도와의 연관성을 확인하기 위해 여고생 596명을 대상으로 조사한 융합연구이다. 데이터는 SPSS 22.0 프로그램을 이용하여 χ2-test와 ANOVA-test 및 Duncan’s test를 시행하였다. 연구결과, 대상자들의 어찬에 대한 기호도 증진 방법에 대한 점수는 ‘먹기 편하게 조리’와 ‘비린내 제거’ 항목의 점수에서 가장 높았다. ‘영양교육’, ‘선호 식품과 배합’, ‘비린내 제거’ 및 ‘선호하는 맛 첨가’ 항목의 점수는 육찬 희망횟수 ‘월 3회 이하‘에서 ‘주 5회 이상‘으로 증가할수록 유의하게 증가하는 경향을 보였다. 이에 육찬의 희망횟수와 어찬 기호도와의 관련성을 확인하였다. 따라서 본 연구는 여고생들에게 생선 기호도 증대를 위한 방향 제시에 도움이 될 것으로 여겨진다.

단일기관에서 조사한 신생아 및 소아 칸디다혈증의 역학적 특성

이택진,전진경,김동수 대한감염학회 2007 감염과 화학요법 Vol.39 No.5

목적 : 최근 신생아 및 소아 칸디다혈증의 위험인자에 따른 원인균의 변화를 알아보고자 하였다. 방법 : 2000년 9월부터 2006년 8월까지 세브란스병원에 입원했던 만 15세 미만의 소아들 중 혈액배양검사에서 칸디다균속이 동정된 환아들의 의무기록을 후향적으로 분석하였다. 결과 : 혈액배양검사에서 칸디다가 동정된 환아는 모두 39명이었으며, 이들의 연령 중앙값은 4개월, 남녀비는 2:1이었고 사망률은 33%였다. 원인균으로는 Candida albicans가 22예(56%), Candida parapsilosis가 9예(23%), Candida glatbrata가 6예(15%), 그리고 Candida tropioalis, Candida famata가 각각 1예씩(3%) 분리되었고, Non-albicans 칸디다들에 의한 칸디다혈증의 비율은 2001년 13%에서 2006년 91%로 점차 증가하는 추세를 보였다(P=0.01). 생후 1개월 미만의 신생아가 12명이었고, 이 중 58%에서 칸디다혈증의 원인균으로 C. parapsilosis가 동정되어 1개월 이상의 소아의 경우(7%)보다 유의하게 흔히 검출되었다(P=0.001). C. albicans가 분리되었던 환아 22명 중 55%에서 칸디다혈증 발병 전 2주 이내에 외과적 시술을 시행받은 경력이 있 어 다른 균주들의 경우(18%)에 비해 높은 비율을 보였다(P<0.05). C. parapsilosis가 분리되었던 환아 9명 중 78%가 미숙아로 출생하여 다른 균주들의 경우(27%)에 비해 높은 비율을 보였다(P=0.015). C. glabrata가 분리되었던 환아 6명 중 67%에서 칸디다혈증 발병 전 2주 이내에 호중구감소증이 있어 다른 칸디다균들의 경우(12%)보다 높은 비율을 보였다(P=0.011). 결론 : 신생아 및 소아 칸디다혈증에서 C. albicans는 외과적 시술을 받은 환아들에서 많이 분리되었으며, C. parapsilosis는 생후 1개월 미만의 신생아, 특히 미숙아에서, C. glabrata는 호중구감소증 환아들에서 많이 분리되었다. Purpose : We evaluated epidemiological and clinical features of candidemia in neonates and children. Methods : We retrospectively reviewed the medical charts of hospitalized neonates and children with positive blood cultures for Candida species from September 1, 2000 through August 31, 2006. Results : Among 39 total neonates and children with candidemia, the median age was 4 months (interquartile range, 1-28) and overall mortality was 33%. Candida species included : Candida albicans (56%), Candida parapsilosis (23%) and Candida glabrata (15%). There was a tendency of proportional increase of candidemia due to non-albicans species (13% in 2001 vs 91% in 2006; P=0.01). Compared with children older than 1 month of age, the proportion of C. parapsilosis was significantly higher in neonates with candidemia (58% vs 7%; P=0.001). C. albicans was isolated more commonly from those who had undergone surgical intervention before candidemia (55% vs 18%; P<0.05). C. parapsilosis was isolated more commonly from premature neonates (78% vs 27%; P=0.015). C. glabrata was isolated more commonly from those who had neutropenia before candidemia (67% vs 12%; P=0.011). Conclusions : Candidemia by C. albicans was more commonly in surgical patients; by C. parapsilosis in premature neonates; by C. glabrata in neutropenic patients.