Transformed E . coli JM 107 에서 순수분리한 S . marcescens 세포외 Nuclease 의 특징

전영환,김기홍,권헌영,신용철,조무제,이상열 ( Young Hwan Jeon,Kee Hong Kim,Heun Young Kwon,Yong Chul Shin,Moo Je Cho,Sang Yeol Lee ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.5

We have previously described the purification procedures of the extracellular nuclease of Serratia marcescens from a recombinant E. coli JM 107 (Jeon and Lee, 1992). Using the purified nuclease, we examined the various biochemical properties of this enzyme. Kinetic parameters of thermal inactivation followed the first order kinetics. Heating the nuclease to 60℃ or higher temperature reduced enzymatic activity. It required 1 mM Mg^(2+) or Mn^(2+) ions for its maximal activity and no other metal ions can be replaced for the cofactor requirement of this enzyme. It was stable at pH in the range of pH 6 to 10, and its maximal activity was exhibited around pH 8. It was resistant to proteolysis by trypsin and an extracellular protease produced from S. marcescens. However, heat-denatured nuclease and bovine serum albumin were completely degraded when they were treated with the same proteases at the same assay conditions. Its activity was significantly inhibited by the addition of 1 mM ethidium bromide.

앉은 자세와 누운 자세에서 외사시각의 변화

전영환,김숙영,Young Hwan Jeon,M,D,Sook Young Kim,M,D 대한안과학회 2006 대한안과학회지 Vol.47 No.2

Purpose: To evaluate changes in exodeviation in the seated and supine position during adjustable suture strabismus surgery. Methods: We measured the deviation angle at distances of 6 m, 2 m, and 33 cm in the seated position and at 2 m, and 33 cm in the supine position by the alternate prism cover test in fifty patients with exotropia. Results: The mean deviation angle was 25.41±7.08 PD at 6 m, 25.51±7.08 PD at 2 m and 28.09±7.54 PD at 33 cm in the seated position. The mean deviation angle in the supine position was 24.68±7.40 PD at 2 m, and 27.29±8.09 PD at 33 cm. There was no significant difference in exodeviation at the same measuring distance between the seated and supine positions. A significant difference between the exodeviation at 6 m in the seated position and the exodeviation at 2 m in the supine position did not exist. Conclusions: The distant deviation angle at 6 m in the seated position can be replaced by the deviation angle measured by fixating a target at the ceiling in the supine position during adjustable suture strabismus surgery for exotropia.

식품의약품안전처 SESSION : 고감도 신속 다종농약 다성분 검색/검사법 개발 연구-LC-MS/MS법

전영환 ( Young Hwan Jeon ),황정인 ( Jeong In Hwang ),김정민 ( Jung Min Kim ),석다롱 ( Da Rong Seok ),이은향 ( Eun Hyang Lee ),전상오 ( Sang Oh Jeon ),김장억 ( Jang Eok Kim ) 한국환경농학회 2013 한국환경농학회 학술대회집 Vol.2013 No.-

현재 세계적으로 자유무역협정(FTA: Free Trade Agreement)으로 인하여 수입식품의 양과 종류 도 매년 지속적으로 증가하여 시중 어디서든지 손쉽게 수입농식품을 구할 수 있다. 수입농산물의 양이 점점 늘어감에 따라 수입농산물의 안전성 평가가 주요한 현안으로 등장하고 있다. 따라서 식 품 안전에 대한 국내외적 경쟁력 확보를 위하여 잔류농약관리의 PLS 체계 도입 필요하다. 본 연구 는 LC-MS/MS를 이용하여 PLS 체계용 고감도 신속 다성분 검색법 개발하고자 한다. 국내에 등록 된 91종 농약의 다성분 동시분석법을 개발하기 위하여 식품공전상의 "다종농약다성분 동시분석법-제2법"에 적용하였다. 현미, 오렌지와 고추시료를 acetonitrile 로 추출한 후, 이를 감압여 과 한 후 여액에 sodium chloride을 넣어주었다. 여과액을 흔들어 섞어 주고 정치하여 acetonitrile층과 물층을 분리시켰다. 상등액인 acetonitrile층 취하여 감압 농축한 후 잔류물을 재용해하여 이를 정제 하였다. SPE cartridge를 이용하여 정제한 후 재용해하여 LC-MS/MS로 분석하였다. 국내미등록 농약 91종 대한 잔류분석법의 벨리데이션을 실시한 결과 대상 분석성분 부근에 시료 중 불순물에 의한 간섭은 관찰되지 않았으며, 검량선의 matrix-matched법으로 사용하였다. 본 연구 에서 확립된 시료조제 및 기기분석과정을 수행하여 얻은 회수율을 산출한 결과는 55∼162%(n=6) 로 나타났으며, 분석오차는 25% 이내로 나타났고, 정량한계는 0.01 mg/kg 이하였다. 본 시험에 의해 확립된 91종에 대하여서는 간단한 모니터링으로는 사용이 가능할 것으로 생각되 며, 앞으로도 나머지 농약에 대하여서 PLS체계를 위한 다성분 분석법 대한 연구가 지속적으로 수행되어야 할 것으로 생각된다.

연구보문 : 환경화학 ; 수입식품 중 국내 미등록 농약의 다성분 잔류분석법 적용

전영환 ( Young Hwan Jeon ),김효영 ( Hyo Young Kim ),황정인 ( Jeong In Hwang ),김지환 ( Ji Hwan Kim ),도정아 ( Jung Ah Do ),임무혁 ( Moo Hyeog Im ),오재호 ( Jae Ho Oh ),권기성 ( Ki Sung Kwon ),이중근 ( Joong Keun Lee ),이영득 ( You 한국환경농학회 2011 한국환경농학회지 Vol.30 No.3

BACKGROUND: Recently in Korea, the import of agricultural products is rising due to the increasing amount of trade. Unregistered pesticides, allidochlor, propachlor, propham, cycloate, diallate and pebulate are widely used as pesticides for rice cultivation in foreign countries, while they are not registered in Korea. Therefore, the residue amount of imported agri-foods should be verified using the proper official analytical method for each of them that has not registered in Korea. METHODS AND RESULTS: This work was conducted to apply the official method of Korea Food & Drug Administration (KFDA) for determining multi class pesticide multiresidues in agricultural commodities. Brown rice and orange which have different characteristics as a matrix were selected as representative samples for residue analysis. The recoveries of cycloate, diallate and pebulate by GC/MS in fortified brown rice and orange with levels of 0.04~0.4 mg/kg were ranged from 82.8% to 110.3%. The quantification limits of three pesticides in brown rice and orange were 0.04 mg/kg. CONCLUSION: As a result, this method can surely be used as an official method for routine analysis of unregistered pesticides in Korea for imported agri-food.

위험인식 및 정부신뢰가 원자력 정책 수용성과 만족도에 미치는 영향에 대한 연구

전영환(Young hwan Jeon),목진휴(Jin W. Mok),김병준(B. Joon Kim) 한국정책분석평가학회 2016 政策分析評價學會報 Vol.26 No.3

There are a number of studies on the determinants toward nuclear energy satisfaction and acceptance. However, there are not many research on the combination among expectancy disconfirmation model (EDM), risk perception and trust in government. In this study, authors develope a path model with the above variables and then come up with a couple of meaningful findings. First, if there is a positive disconfirmation between expectation and performance on nuclear energy policy, trust in government and the level of acceptance on nuclear energy is increased. Second, if the level of individual citizen’s risk perception is increased, the acceptance level is decreased. In particular, the higher positive perception on governmental performance reduces the level of disappointment on governmental nuclear policy as well as reduces the level of fear on nuclear energy. At the same time, the positive perception influences on the increase of trust in government. Therefore, the government policies should be implemented in terms of long-term perspective and incremental manner rather than expecting short-term and quick solutions for the nuclear energy policy.

국내 미등록 유기인계 농약의 수입 농식품에 대한 다성분 잔류분석법

전영환 ( Young Hwan Jeon ),황정인 ( Jeong In Hwang ),안지운 ( Ji Woon Ahn ),김효영 ( Hyo Young Kim ),도정아 ( Jung Ah Do ),오재호 ( Jae Ho Oh ),황인균 ( In Gyun Hwang ),임무혁 ( Moo Hyeog Im ),이중근 ( Joong Keun Lee ),이영득 ( Yo 한국환경농학회 2012 한국환경농학회지 Vol.31 No.3

외국으로부터 수입되는 농식품에 대하여 국내에 미등록 된 유기인계 농약 aspon, chlorthion, chlorthiophos, crotoxyphos, demeton-O, demeton-S, demeton-S-methyl, dioxathion, heptenophos, iodofenphos, leptophos, methyl-trithion, propetamphos 및 sulfotep 등 14종에 대한 안전성 평가를 위하여 다성분 잔류분석법을 확립하고자 하였다. 잔류분석법은 식품의약품안전청에서 고시한 다종농약다성분 동시분석법-제2법에 잘 적용되었다. 수입 농산물의 대표 시료로 선정된 현미와 오렌지에 대한 유기인계 농약 14종 대한 잔류분석법의 밸리데이션을 실시한 결과 특이성, 직선성, 정확성, 정밀성 및 정량한계 수준을 만족시키는 것으로 나타났다. 단지 crotoxyphos의 경우는 기기상의 정량한계가 낮아서 저농도에서의 회수율은 좋지 않은 결과를 나타내었다. 따라서 본 연구에서 확립된 다성분 잔류분석법은 수입 농산물 중 crotoxyphos를 제외한 aspon외 12종의 유기인계 농약에 대해 적용 가능한 것으로 나타났다. BACKGROUND: For safety evaluation of imported agri-food in Korea, the multiresidue analysis method was establised for unregistered organophosphorus pesticides, aspon, chlorthion, chlorthiophos, crotoxyphos, demeton-O, demeton-S, demeton-S-methyl, dioxathion, heptenophos, iodofenphos, leptophos, methyl-trithion, propetamphos and sulfotep. METHODS AND RESULTS: The used method for multiresidue analysis in brown rice and orange used as representative samples of imported agri-food was the official method of Korean Food and Drug Administration. The results of validation test of 13 organophosphorus pesticides except crotoxyphos for multiresidue analysis method are compared to the criteria such as specificity, linearity, accuracy, precision and limit of quantification. CONCLUSION: The used method for multiresidue analysis of unregistered 13 organophosphorus pesticides except crotoxyphos in Korea can surely be used as an official method for routine analysis of imported agri-food.

농산물에 대한 LC-MS/MS를 이용한 농약의 다성분분석법 확립

전영환 ( Young Hwan Jeon ),황정인 ( Jeong In Hwang ),김정민 ( Jung Min Kim ),석다롱 ( Da Rong Seok ),이은향 ( Eun Hyang Lee ),전상오 ( Sang Oh Jeon ),도정아 ( Jung Ah Do ),오재호 ( Jae Ho Oh ),홍진환 ( Jin Hwan Hong ),이영득 ( You 한국환경농학회 2013 한국환경농학회 학술대회집 Vol.2013 No.-

Pest control is major problem in front of farmer worldwide, an enormous economic loss is observed due to pest every year. There are several chemical pesticides available for controlling agricultural pests. Studies have shown chemical pesticides can cause significant health risks to humans, contaminate water supplies, and to plant itself. To come through this problem, we previously developed some natural pesticides i.e. oak pyroligneous liquor, rice bran and phytic acid which were tested against Perilla frutescens rust disease. We found good pesticide capacity of these natural extracts, so we were veracious to know whether it has toxic effect or not for that we designed the present investigation. In the present study we have determined toxicity on the basis of fish survival assay, seed germination assay and mice feed assay. Briefly, a concentration of 50 and 100μg\ml of all pesticides were supplied in water of fishes for 96hrs. We have taken two kind of fishes for this experiment Misgurnus anguillicaudatus(10) and Cyprinus carpio(10). After completion of the incubation period number of dead fish were calculated. Simultaneously, we determined seed germination test on four types of seed Cabbage, Radish, Black beans and Red kidney beans. A number of 20 seeds in case of red kidney beans 5 seeds were kept in sterile Petri dishes bedded with layer of tissue papers (Kimtec) which were wet with autoclaved distilled water. Treatment of pesticides was done with a concentration of 50 and 100μg\ml for 48 to 96 hrs. The number of non-germinated seeds was counted after the incubation period. In the case of animal toxicity test we supplied pesticides in the drinking water of mice and they were kept under investigation of body weight and death of mice. The results obtained from fish experiment suggest no toxicity of oak and rice bran but at a higher concentration one fish was found dead. In the case of seed germination assay all the seeds were observed to be germinated at both concentrations for all pesticides. No decrease in body weight was found in mice experiment as well as all mice were live. In accordance with all data, we concluded that the use of Oak, Rice bran and Phytic acid as a pesticide is safe and effective especially in the case of Perilla rust disease.

현미와 오렌지에 대한 국내 미등록 31농약의 다성분 분석법 확립

전영환 ( Young Hwan Jeon ),황정인 ( Jeong In Hwang ),안지운 ( Ji Woon Ahn ),김정민 ( Jung Min Kim ),석다롱 ( Da Rong Seok ),이은향 ( Eun Hyang Lee ),도정아 ( Jung Ah Do ),오재호 ( Jae Ho Oh ),임무혁 ( Moo Hyeog Im ),이중근 ( Joong 한국환경농학회 2012 한국환경농학회 학술대회집 Vol.2012 No.-

살충제 Flubendiamide의 복숭아 품종에 따른 잔류양상

김효영 ( Hyo Young Kim ),황정인 ( Jeong In Hwang ),이은향 ( Eun Hyang Lee ),전영환 ( Young Hwan Jeon ),김지환 ( Ji Hwan Kim ),안지운 ( Ji Woon Ahn ),박현주 ( Hyun Ju Park ),정창국 ( Chang Kook Chung ),김산영 ( San Yeong Kim ),이 한국환경농학회 2012 한국환경농학회지 Vol.31 No.2

표면의 형태가 다른 복숭아 3품종을 선정하여 살충제 flubendiamide의 잔류양상을 조사하고 또한 전착제 사용에 따른 잔류량을 조사하였다. 복숭아 표면의 형태는 현미경으로 관찰한 결과 창방, 월미 품종의 순으로 털이 많았으며, 천홍품종은 털이 없는 것으로 나타났다. Flubendiamide의 잔류량은 털이 많은 창방에서 가장 높게 나타나 0.54 mg/kg이었으며, 월미에서 0.43 mg/kg, 그리고 천홍에서 0.10 mg/kg이었다. flubendiamide와 전착제 polyoxy ethylene methylpoly siloxane를 함께 처리를 하였을 때, 각 품종별 복숭아에 대한 농약 잔류량은 창방에서는 전착제의 사용유무에 관계없이 0.55 mg/kg로 거의 변화가 없었으며, 월미에서는 0.53mg/kg으로 0.10 mg/kg 증가하였다. 천홍에서는 0.48mg/kg으로 나타나 전착제의 사용으로 잔류량이 4.8배 증가되었다. 이러한 결과는 전착제의 사용이 표면에 털이 있는 품종에서는 농약의 잔류량을 증가시키지 않았으나, 표면에 털이 없는 품종에서는 잔류량을 증가시키는 것을 보여주었다. BACKGROUND: This research has investigated the residue patterns of insecticide flubendiamide on three species of peaches with different surface forms, and the residue amounts of them when mixed with a spreader. METHODS AND RESULTS: Pesticide used for field application on peaches was 20% flubendiamide of suspension concentrate (SC) and was sprayed at a recommended rate. The residue amounts of flubendiamide in peach were analyzed by HPLC equipped with UV detector. After the observation with a microscope, the rank of fuzz amount on peach`s surface was Kurakatawase, Wolmi in descending order and Cheonhong did not have any fuzz. The residue amounts of flubendiamide were 0.54mg/kg for Kurakatawase, 0.43 mg/kg for Wolmi and 0.10mg/kg for Cheonhong, respectively. When flubendiamide was used with a spreader, polyoxy ethylene methylpoly siloxane, the residue amount for Kurakatawase barely changed at 0.55 mg/kg regardless of mixing with a spreader, and at 0.53 mg/kg for Wolmi. In Cheonhong, the residue amount was 0.48 mg/kg, which increased by 4.8 times due to the use of a spreader. CONCLUSION: This result indicates that the residue amounts of flubendiamde were affected by the surface forms of peaches, and in the presence of a spreader the residue amount did not increase in fuzzy species, but was affected greatly for species without fuzz.

연구보문 : 환경화학 ; 유통 중인 관행 및 인증 농산물 중 곡류와 엽채류의 잔류농약 모니터링 및 안전성 평가

김효영 ( Hyo Young Kim ),전영환 ( Young Hwan Jeon ),황정인 ( Jeong In Hwang ),김지환 ( Ji Hwan Kim ),안지운 ( Ji Woon Ahn ),정덕화 ( Duck Hwa Chung ),김장억 ( Jang Eok Kim ) 한국환경농학회 2011 한국환경농학회지 Vol.30 No.4

BACKGROUND: This study was conducted to monitor the current status of pesticide residues and to assess their risk in domestic agricultural products. The samples were rice, barley, lettuce and perilla leaf. These four types of agricultural products were those with GAP(Good Agricultural Practice) certification, organic agricultural products, pesticide-free agricultural products or general agricultural products. METHODS AND RESULTS: They were purchased from traditional markets and supermarkets of 12 regions in Korea from July to August 2010. The total number of samples was 259 for agricultural products and these were analyzed by GC/ECD, GC/NPD and GC/MSD. We used multiresidue methods to analyze for 110 different pesticides except for herbicides. CONCLUSION: In this study, residual pesticides were detected in 18 samples. Among these general agricultural products, organic agricultural products and products with GAP-certification were detected in 12, 4 and 2 samples, respectively. Detection rates of general agricultural products, organic agricultural products and products with GAP-certification were 4.6%, 1.5% and 0.8% respectively. Pesticides were not detected in pesticide-free agricultural products. Their detection levels were less than their maximum residue levels. Their estimated daily intakes ranged from 0.0003% to 0.04302% of their acceptable daily intakes, of which the values have no effect on human health.