질환모델마우스 생산을 위한 체세포핵이식방법 개선 1 : 탈핵 및 재조합난자 생산기법 최적화

전수현,심호섭,정형민,이병천,이은송,고정재,신태형,박찬,황우석 韓國受精卵移植學會 2000 한국동물생명공학회지 Vol.15 No.3

This study was undertaken to optimize enucleation and reconstitution methods for the production of cloned mice by somatic cell nuclear transfer Outbred ICR mouse oocytes at the metapahse- II stage were retrieved from female mice superovulated by PMSG and hCG. In Experiment 1, oocytes were enucleated in medium supplemented with cytochalasin B (CCB) of 3 levels (0, 7.5 or 15 /mL), and higher rate of encleation was obtained at 7.5 and 15 /mL than at /mL. In Experiment 2, oocytes enucleated in 7.5 /mL CCB-containing medium were reconstituted with different types of somatic cell by following methods; 1) cumulus cells by direct cell injection, 2) cumulus cells by electric fusion (1.25 kV/cm, 2 pulses for each 70 ) or 3) STO cells by the electrofusion. Electrofusion of STO cells with enucleated oocytes yielded the greatest (P<0.05) rate of reconstitution without lysis (76%) than any other combinations. Although significant decrease in the rate of somatic cell introduction was found, the electrofusion of cumulus cells yielded better rate of reconstitution than direct injection (0 vs. 18%). In Experiment 3, the duration of electric stimulation for the fusion was changed to either 50 or 90 , but no significant improvement of reconstitution efficacy was obtained. In conclusion, this study showed that ICR mouse oocytes could be used for the production of reconstituted oocytes and a fusion method of 1.25 KV/cm with 2 pulses using 570 cell was the optimal.

Sherlock 소프트웨어를 이용한 큐브위성용 전자보드의 고장 메커니즘 별 신뢰성 및 수명예측

전수현,권형안,임인옥,오현웅 한국항공우주학회 2015 한국항공우주학회 학술발표회 논문집 Vol.2015 No.11

상용급 위성에 탑재되는 전장품의 경우 고 신뢰성을 만족시키기 위해 우주급 부품을 사용하지만 큐브위성은 저가/단기간 개발목표 충족을 위해 진동 및 열환경 규격을 만족하는 상용부품을 적용하게 되며 적용 부품에 대하여 최소한의 검증시험만을 실시하고 발사 및 궤도 운용을 실시한다. 하지만 지상에서 실시되는 제한된 환경시험만으로 장시간에 걸친 궤도상 열진공과 같은 물리적 부하 환경에 노출된 전자보드의 신뢰성을 보장할 수 없으며, 이를 보상하기 위해 장시간의 시험을 수행하는 것은 막대한 비용과 시간이 소요되므로 효율적이지 않다. 본 논문에서는 STEP Cube Lab.의 주요 임무보드인 MEMS 추력기 제어보드를 수명예측 분석 대상으로 신뢰성 수명예측 상용도구인 Sherlock 을 통해 발사 및 궤도환경에서의 임무보드의 고장 메커니즘 별 수명예측 및 임무 기간 동안의 신뢰도를 분석하였다. Electric units for the commercial satellite generally use space-grade components to satisfy the high reliability requirement. In case of cube satellite applications, however, they used to apply commercial-grade components that satisfy the vibration and thermal environmental specifications and goes into mission orbit even after undergoing minimum environmental tests due to their low cost and short development period. However, its reliability exposed to the physical environment such as extreme on-orbit thermal vacuum for long periods cannot be assured under minimum tests criterion. In addition it is undesirable to perform long period environmental tests for compensating its reliability. In this paper, we have analyzed the reliability and life prediction of the failure mechanisms of the MEMS thruster control board, which is main mission board of STEP Cube Lab. The analysis was performed by using the Sherlock software of reliability and life prediction tools under the launch and on-orbit environment.

기간별 주가데이터를 이용한 LTSF-Linear 모델 성능비교

전수현,이호정,이민상,이주희,우다경,이충권 한국경영정보학회 2023 한국경영정보학회 학술대회논문집 Vol.2023 No.11

주가는 기업의 가치를 반영하고 있는 대표적인 지표이다. 주가의 예측은 기업의 영업실적외에 타 요인도 존재한다. 본 연구는 Linear Linear Linear Linear Linear Linear Linear 기반 모델을 활용하여 예측 기간별 성능이 가장 뛰어난 모델을 파악하고자 한다. 2022년 12 월 31 일 기준 시가총액 상위 5개 기업을 선별하여 3년, 5년, 7년 종가 데이터를 수집했다. 예측결과 3년 데이터는 D-Linear, 5, 7년 데이터는 N-Linear 모델의 성능이 가장 우수했다. 두 모델은 COVID -19 라는 변수에도 불구하고, 예측성능이 뛰어난 시계열 예측 모델임을 확인하였다.

사회적 배제 경험이 소비자의 신기술 사용 의도에 미치는 영향

전수현,정혜욱 한국유통경영학회 2022 유통경영학회지 Vol.25 No.3

Purpose: We can easily experience social exclusion in our daily lives. This study was conducted to confirm the effect of social exclusion on the intention to use new technologies (e.g., VR, MR, or autonomous vehicle) in a rapidly changing modern society and the mediating effect of prevention focus in the process. Research design, data, and methodology: To test our hypotheses, we conducted two experiments. In experiment 1, we manipulated social exclusion and performed a between-subject design. We empirically tested the hypothesis using logistic regression. In experiment 2, one hundred twenty-one subjects participated. To investigate prevention focus as a mediator, the data was analyzed using Process model 4 with 5000 bootstrap samples. Results: The results of the experimental study demonstrated that the group who experienced social exclusion showed less intention to use new technologies than the group who experienced social inclusion. In addition, we found that the experience of social exclusion affects consumers’ prevention focus, reducing the intention to use new technologies. Results: The results of experimental study demonstrated that the group who experienced social exclusion showed less intention to use new technologies than the group who experienced social inclusion. In addition, we found that the experience of social exclusion affects consumers’ prevention focus, reducing the intention to use new technologies. Implications: The present study contributes to new technology acceptance literature, showing that psychological factors such as social exclusion play a vital role in the adoption of new technologies. Psychological factors have received little attention concerning the adoption of new technologies. Also, from a managerial aspect, with the growth of the market and shortening of technologies’ lifecycle, it is important to understand the factors influencing consumer adoption of new technologies.

북한이탈주민에 대한 청소년의 인식 분석과 다문화교육 방향 모색

전수현 부산외국어대학교 다문화연구소 2018 다문화사회와 교육연구 Vol.1 No.-

Korea is the only divided nation in the world. According to the preliminary report of the Ministry of Unification in September 2017, the total number of defectors is 31,093. In order for South Korea to overcome the conflicts between the two Koreas and to prepare and achieve unified Korea in the near future, it is very necessary to understand and study the reality of North Korean defectors and their suffering due to alienation, discrimination and prejudice that they feel in South Korean society. This will be reflected and helped in the multicultural research and education of Korea, which is rapidly progressing into a multicultural society. The number of North Korean refugees is increasing and is being influxed into South Korean society by various age groups such as age and education. As a result, education and systems for North Korean defectors are available, but no education is provided at the educational sites related to their understanding and North Korean defectors. It is enough that the school district grasps the number of students at each school every semester. In fact, most young people in middle and high school do not even know the term "defectors", and they do not know what reason and process they come to Korea to settle and live in. Most of them do not know about the reality of defectors. In this study, we interviewed 8 middle and high school students in Busan ​​city to find out how young people think about defectors, what kind of educational and understanding efforts they have made, It aims to find educational implications for students in society.

전자상거래 환경하에서 공정성과 진정성이 회복만족에 미치는 영향

전수현,곽기영 대한산업공학회 2015 대한산업공학회 춘계학술대회논문집 Vol.2015 No.4

With the rapid growth of the internet, electronic commerce has become accepted as one of the major purchasing channels for consumers. As more and more consumers search for and purchase products through the internet, intra-industry competition of electronic commerce has become fierce Therefore, electronic commerce service providers pay attention to factors that prevent existing customers from leaving there services in the service failure situation In this study, we suggest that procedural justice, interactional justice, distributive justice and authenticity have positive inpacts on recovery satisfaction. In addition, we also propose that involvement plays moderating roles in the relationships between procedural justice, interactional justice, distributive justice, authenticity and recovery satisfaction. The emprirical results support the proposed model and partly identify the moderating effect of involvement differences. We expect that this result will provide researchers and managers who are interested in electronic commerce service failure recovery with useful theoretical and practical implications.

영국 어린이들의 장소애착심, 만족도 및 브랜드 충성도가 맥도날드에 대한 태도 및 재구매 의도에미치는영향

전수현 한국경영교육학회 2014 한국경영교육학회 학술발표대회논문집 Vol.2014 No.6

전자상거래 환경에서의 서비스 실패 회복 과정에 관한 연구

전수현,곽기영 한국경영정보학회 2014 한국경영정보학회 학술대회논문집 Vol.2014 No.1

본 연구를 통해 전자상거래 환경하에서 서비스 실패 후 회복 과정에 대한 공정성과 진정성이 회복 만족에 미치는 영향력을 분석하고자 한다. 더불어, 공정성이라는 인지적 요소와 진정성이라는 감정적 요소가 미치는 영향력이 제품의 관여도라는 조절 효과에 따라 어떠한 차이가 있는지를 살펴보고자 한다. 또한, 서비스 실패 회복 과정에서 공정성의 3가지 하위 요소의 가중치도 파악해 볼 수 있으며, 회복 만족을 이끄는 주요한 선행 변수도 이끌어 낼 수 있을 것이다. 나아가 서비스 회복 만족이라는 태도 요인이 추천의도 및 재구매의도와 같은 행동 요인을 촉발해낼 수 있는지에 대해서도 파악해 볼 수 있다. 본 연구는 전자상거래 환경하에서 서비스 회복 노력에 대한 지각된 공정성과 진정성이 회복 만족에 미치는 영향력의 결과를 실증적으로 보여줌으로써 서비스 기업들에게 보다 구체적인 방향성을 제시할 수 있을 것이고, 회복 노력의 중요성에 대한 인식을 제고시켜 줄 수 있을 것이다.

시중 일반우유와 포름알데히드로 보호 처리된 어유 첨가 사료를 먹인 DHA 강화우유의 안전성 연구

전수현,이광원,남미현,홍충의,양성용,유진아,서동원,정일중 한국식품위생안전성학회 2011 한국식품위생안전성학회지 Vol.26 No.4

Our objective in this study is to assess the safety of docosahexaenoic acid (DHA) fortified milk of dairy cows fed feeds containing protected fish oil treated with formaldehyde by analyzing formaldehyde concentration in commercial milk and DHA fortified milk of dairy cows fed formaldehyde treated feed. There are 3 milk samples in this study: Commercial milk (CM), DHA fortified milk for Kid (DHA-K) and DHA fortified milk for Baby (DHA-B). We confirm the fresh quality of these three samples by physicochemical tests. In fat content result, three groups are significantly different at the p < 0.05 by Duncan's multiple range test, but fat content of group DHA-K is about half the level of the other two groups. Protein content of group DHA-K is 1% higher than other two groups. According to the analysis result of DHA content of DHA fortified milk, DHA content of DHA-B is two-fold higher than DHA-K. Similar pattern was seen in the intake based on age. According to HPLC analysis result of formaldehyde concentration in milk, commercial milk and DHA fortified milk are between 0.013 ppm and 0.057 ppm which is formaldehyde standard level in fresh milk settled in WHO (World Health Organization). Three groups have no significantly differences at the p < 0.05 by Duncan’s multiple range test. For this reason, it can be concluded that there is no transition of formaldehyde from dairy cows fed formaldehyde treated feeds to its produced milk. Safety about formaldehyde of DHA fortified milk of dairy cows fed formaldehyde treated feeds is considered similar to commercial milk.

Impacts of SNS Attachment and Travel Product Satisfaction on Motivations for Sharing Product Experiences on SNSs

전수현,노정희 (사)한국관광레저학회 2015 관광레저연구 Vol.27 No.4

This study investigated main and interaction effects of SNS attachment and travel product satisfaction on motivations for sharing product experiences on SNSs. The SNS attachment and the travel product satisfaction were significantly positively associated with motivations for knowledge distribution, knowledge storing, nostalgia, enjoyment and social interaction. The SNS attachment was significantly positively associated with motivations for symbolic expression and identity seeking. Results of further analyses indicated that when people had the higher levels of the SNS attachment and product satisfaction, they were more likely to share information on SNSs to express positive feelings, compared to those who had the lower levels of the SNS attachment and product satisfaction. When people had a higher level of the SNS attachment and a lower level of product satisfaction, they were more likely to share information on SNSs to express negative feeling. However, when they had a lower level of the SNS attachment, they were less likely to express negative feeling on SNSs regardless of product satisfaction. Theoretical and managerial implications as well as limitations of the study were discussed.