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      • KCI등재후보

        보문 : Blemish Balm 크림에서 색조를 나타내는 산화철의 미세구조

        전명옥 ( Myung Ok Jeon ),장병수 ( Byung Soo Chang ) 대한미용과학회(구 한국두피모발미용학회) 2010 대한미용학회지 Vol.6 No.2

        This study investigated the structure and size of particles of iron oxides which provide shade to the skin among the elements of blemish balm cream, which is recently stirring up a boom in the cosmetic market. We observed that the rod-shaped particles of iron oxide red are dispersed in lumpy form. Under a high magnification scanning electron microscopy, we observed that the rod-shaped iron oxide red 500~600 nm in length and the cubicshaped iron oxide red 100 nm in length are dispersed. The particles of iron oxide yellow were in a form of rod and their length was measured as 300~600 nm. The particles of iron oxide black formed irregular masses and were in a spherical form and their diameter was measured as 200~300 nm. When the results of analyzing the elements of iron oxide red using an energy dispersive X-ray spectroscopy were converted into a percentage, the components of Fe and O was 74.66% and 25.34%, respectively. For iron oxide yellow, the components of Fe and O was 75.00% and 25.00% and for iron oxide black, the components of Fe and O was 84.78% and 15.22%, respectively. As a result, it was found that iron oxide red, iron oxide yellow and iron oxide black, as one of the ingredients of cosmetics, were fine particles which made up of the element of Fe and O with no impurities.

      • KCI등재

        잣나무(Pinus koraiensis Siebold et Zucc)잎 추출물의 화장품 소재로서의 활용 가능성에 관한 연구

        전명옥(Myung Ok Jeon),문지선(Ji sun Moon) 한국유화학회 2017 한국응용과학기술학회지 Vol.34 No.3

        본 연구에서는 잣나무 잎으로 70% 에탄올 추출물과 증류수 추출물을 얻은 후 항산화 효과, 항염증, 미백효과 등 다양한 효과를 검증하고자 연구를 수행하였다. 항산화 실험 결과, 폴리페놀, 플라보노이드 효과에서는 폴리페놀과 플라보노이드 함량이 농도 의존적으로 증가하였다. 폴리페놀과 플라보노이드 함량은 증류수 추출물에 비해 에탄올 추출물에서 높은 것으로 확인되었다. B16F10, RAW264.7 세포에서 농도별로 세포독성이 나타나지 않았으며, NO 생성 억제능 측정 결과, LPS로 유도된 NO 생성을 효과적으로 억제하여 항염증 활성이 있을 것으로 예측되었다. 멜라닌 생합성 억제능 측정결과, 유의한 감소효과를 확인하였으며, western blot을 수행하여 MITF, Tyrosinase 단백질 발현억제 실험결과, 농도 의존적으로 현저히 억제됨을 확인할 수 있었다. 따라서 본 연구결과로부터 잣나무 잎추출물은 화장품소재로서의 다양한 활용 가능성이 있을 것으로 사료된다. This study was performed to verify various effects of 70% ethanol extract and distilled water extract of pine leaf such as antioxidant, anti-inflammatory, skin-lightening effects analysis in cells. The result of antioxidant experiment showed that polyphenol content increased concentration dependently in polyphenol and flavonoid effects. The total content of polyphenols and flavonoids was much higher in ethanol extract than water extract. In all the cells of B16F10, RAW264.7 cytotoxicity at each concentration level didn t appear. The result of measuring NO production inhibition showed that it inhibited NO production led to LPS effectively, so anti-inflammatory activity was confirmed. The result of measuring melanin biosynthesis inhibition showed that there was considerably reduction effect, but it performed western blot, and as a result of MITF, Tyrosinase protein revelation, the inhibition of MITF, Tyrosinase revelation was confirmed concentration dependently, Therefore, in this study pine leaf extract was expected to be used as a cosmetic ingredient.

      • KCI등재

        화장품에 사용되는 백색분체의 미세구조와 실리카의 특성

        전명옥,장병수,Jeon, Myung-Ok,Chang, Byung-Soo 한국현미경학회 2012 Applied microscopy Vol.42 No.2

        본 연구는 색조화장품에 사용되는 실리카, 이산화티탄, 탈크, 카올린과 BB크림 화장품에서 분리한 혼합물의 미세구조를 주사전자현미경을 사용하여 관찰하였다. 카올린은 판상의 다각형 구조로 매끄러운 표면을 가지고 있었고 가장자리는 탈크의 경계면과 비교하였을 때 상대적으로 부드러운 모양을 하고 있었다. 또한 여러 겹의 층으로 형성된 덩어리에서 각 층의 두께는 약 $0.1{\mu}M$로 측정되었다. 탈크는 아주 얇은 박편상으로 이들 박편들이 층을 이루고 있는 덩어리 형태로 관찰되었다. 이들 얇은 판상의 탈크 경계면은 뾰족하거나 각이진 형태로 관찰되었으며 박편두께는 약 600 nm로 관찰되었다. 카올린과 탈크의 박편두께를 비교하였을 때 카올린 박편의 두께가 두꺼운 것으로 나타났다. 이산화티탄은 직경이 0.2~0.3 ${\mu}M$로 측정되었고 입자 표면이 부드러운 입방형의 형태를 하고 있었다. 실리카는 구형의 형태로 크기가 200 nm에서부터 $15{\mu}M$까지 아주 다양한 크기로 관찰되었다. BB크림 화장품에서 무기안료들을 관찰한 결과 실리카는 균질하게 분산되어 있었고 이들 사이에 크기가 상대적으로 작은 탈크, 카올린, 이산화티탄, 산화철 등이 채워져 있었다. 결론적으로 색조화장품에서 실리카는 피부에 화장막을 형성하는 최소한의 두께로 작용한다. In this study, fine structures of silica, titanium dioxide, talc and kaolin used in decorative cosmetics and the mixture extracted from BB cream cosmetics were observed by scanning electron microscopy. Kaolin had plate like shape structures of polygon with smooth surface and edge of kaolin had a relatively smooth appearance in comparison with talc. Also, thickness of each layer was estimated to about $0.1{\mu}M$ in the lump formed in stratum of several layers. Talc was observed by lumps shape phase of layering very thin flake. Boundary of thin flake was sharp or angular phase and thickness of flake was approximately 600 nm in diameter. When comparing the thickness of kaolin and talc, we was confirmed that kaolin was thicker than talc. Diameter of titanium dioxide was estimated to 0.2~0.3 ${\mu}M$ and surface of particle was a soft cubic form. Silica was confirmed that variety of size from 200 nm to $15{\mu}M$ of globular shape was measured. From the observation of inorganic pigments, silica was homogeneous dispersed in the BB cream cosmetics and among each other was filled with relatively small size like talc, kaolin, titanium dioxide and iron oxide. In conclusion, we suggest that silica at decorative cosmetics were formed in cosmetic coat at the skin as the minimum thickness.

      • KCI등재후보

        보문 : Hydrogen Peroxide로 손상된 배양 인체피부섬유모세포에 대한 함초추출물의 항산화 및 항독효과에 관한 연구

        최유선 ( Yu Sun Choi ),김선주 ( Sun Ju Kim ),전명옥 ( Myung Ok Jeon ),유영월 ( Young Wall Ryu ),임요섭 ( Yo Sup Rim ),정인주 ( In Ju Jung ) 대한미용학회(구 대한미용과학회) 2011 대한미용학회지 Vol.7 No.4

        This study aims to evaluate the antioxidative effect and the detoxic effect of Salicornia herbacea L. (SH) extract. For the examining of antioxidative effect on SH extract, the cell viability of freeze-dried SH (FSH) extract or cold-dried SH (CSH) extract was measured after the cultured human skin fibroblasts (Detroit 551) were pretreated with SH extract for 2 hours before the treatment of hydrogen peroxide (H2O2) and also the inhibitory activity of lipid peroxidation was assessed. In the detoxic effect of SH extract, the cell viability was measured by colorimetric assay after Detroit 551 cells were pretreated with FSH extract or CSH extract before the treatment of H2O2. In this study, H2O2 significantly decreased cell viability in dose-dependently and the XTT90 and XTT50 values were determined at 15 μM and 40 μM of H2O2, respectively. In the protective effect of FSH extract against H2O2-treated group, FSH extract significantly increased cell viability, compared with H2O2-treated group, and also FSH extract showed the inhibitory activity of lipid peroxidation. While, in the detoxic effect of SH extract, FSH extract significantly increase cell viability which were decreased by methylmercuric chloride (MMC)-induced cytotoxicity, but CSH extract did not show any protective effect. From these results, it is suggested that H2O2 was highly toxic and SH extract such as FSH extract was effective in the prevention of H2O2- or MMCinduced cytotoxicity by antioxidative effect or detoxic effect. Conclusively, FS extract may be a useful agent for the beauty materials by the protective effect on a bleaching agent, H2O2 or the heavy metal as the pigment of color make-up product

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