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Serotyping in Patients with Chronic Hepatitis C
장순모 THE KOREAN SOCIETY FOR BIOMEDICAL LABORATORY SCIEN 2003 Journal of biomedical laboratory sciences Vol.9 No.4
To determine the clinical usefulness of Immuno Blot test, 160 samples from the patients with chronic HCV infection were analyzed. And serotyping and line probe assay were performed to evaluate the distribution of hepatitis C virus genotypes in Korean isolates. In this group, as a result of genotyping type 1b and 2a, the serotype Ⅰ and Ⅱ were the most common source of HCV infection. There were no significant difference in response to the alpha-interferon HCV infection treatment with the subtype 1b or 2a. And the serotypes of NS4 peptides were compared with the genotypes to evaluate their clinical usefulness. Among 49 cases studied for genotypes and serotype, genotype 1b, 1b/2b, 2a, 2a/2c and 2b were 51.0%, 2.0%, 34.6%, 8.1% and 4.0%, respectively. The serotypes Ⅰ and Ⅱ were 57.1% and 42.8%, respectively; they were matched with genotypes in 85.7% and seemed to be easy to perform. To monitor their performing progress or treatment response, serotype test was made before the genotype test. The Result showed that there was no significant difference in response to the alpha-interferon HCV infection treatment with the subtype 1b or 2a in Korea.
만성 C형 간염바이러스 진단에 있어서 HCV검사법의 평가
장순모 ( Soon-mo Jang ),양병선 ( Byoung Seon Yang ) 대한임상검사과학회 2018 대한임상검사과학회지(KJCLS) Vol.50 No.3
만성C형 간염환자 160예를 대상으로 immunoblot방법과 RT-PCR-hybridization법을 실시하여 임상검사의 유용성을 알아보았다. 양성 150예 중 133예만 RT-PCR-hybridization 법에서 양성을 나타내어 immunoblot법의 진양성율은 88.6%를 나타났으며 두 방법 간의 일치율은 89.3%를 나타났다. 한국인의 HCV 아형을 알기 위하여 혈청형과 유전형을 실시하였다. HCV혈청형 1형과 2형 그리고 1b와 2a유전형이 가장 많은 C형간염 감염원으로 나타났다. 49예를 혈청형과 유전형을 서로 비교한 결과, 혈청형 검사에서 28예(57.1%)가 1형, 21예(42.9%)가 2형으로 나타났으며 유전자형 검사에서 1b형이 25예(51.0%), 1b/2b를 나타낸 예가 1예(2.0%), 2a형이 17예(34.7%), 2a/2c를 나타낸 예가 4예(8.2%), 그리고 2b형이 2예(4.1%)로 나타났다. 본 연구에서는 HCV 간이검출법으로는 immunoblot방법이 유용하며, immunoblot방법 양성 확진검사로는 RT-PCR-hybridization법을 실시하였다. 혈청형이 C형간염의 치료 나 진행과정을 관찰하기 위해서는 유전형보다 유용하나 인터페론 치료효과는 1형과 2형 혈청형별에 따라 차이를 보이지 않았다. To determine the clinical utility of an immunoblot test and RT-PCR-hybridization test, 160 samples from patients with a chronic HCV infection were analyzed by two tests. A total of 133 samples out of 150 positive samples were positive by RT-PCR-hybridization. The true positive rate of the immunoblot tests and the concordance rate of the two tests was 88.6% and 89.3%, respectively. Serotyping and genotyping were performed to evaluate the distribution of the HCV subtype in Korean isolates. HCV serotypes 1 and 2, and genotypes 1b and 2a were the most common sources of HCV infections in this group. In 49 cases studied with the serotypes and genotypes, serotypes 1 and 2 were 57.1% and 42.9%, respectively. Genotypes 1b, 1b/2b, 2a, 2a/2c, and 2b were 51.0%, 2.0%, 34.7%, 8.2%, and 4.1%, respectively. This study shows that immunoblot tests are more useful for screening HCV infections. The RT-PCR-hybridization test confirmed the HCV infection in patients with positive immunoblot test results. The serotype test is preferred over the genotype test for monitoring the progression or response to treatment. On the other hand, there were no significant differences in the response to an α-interferon treatment of HCV infection with serotype type 1 or type 2 in Korea.
Polymerase Chain Reaction-Sequence Specific Primer를 이용한 HLA-B 유전자의 DNA 다형성 조사
장순모 ( Soon Mo Jang ) 대한임상검사과학회 2007 대한임상검사과학회지(KJCLS) Vol.39 No.3
Most expressed HLA (human leukocyte antigen) loci exhibit a remarkable degree of allelic polymorphism, which derives from sequence differences predominantly localized to discrete hypervariable regions of the amino terminal domain of the molecule. In this study, the HLA-B genotypes were determined in twenty students unrelated koreans using the PCR-SSP (polymerase chain reaction-sequence specific primer) technique. Several specific primer pairs in assigning the HLA-B gene were used (B*4001/4007, B*4901/5001/4501, B*3701, B*5801). The results of PCR-SSP, the HLA-B3701 primer was detected one (5%), the HLA-B*5801 were detected four (20%), the HLA-B*4001/4007 were detected nineteen (95%) and the HLA-B*4901/5001/ 4501 were detected twenty. This study shows that the PCR-SSP technique is relatively simple, fast and a practical tool for the determination of the HLA-B genotypes. Moreover, these results genotype frequency of the HLA-B gene could be useful for database study before being applied to individual identification and transplantation immunity.
장순모 ( Soon Mo Chang ) 대한임상검사과학회 2000 대한임상검사과학회지(KJCLS) Vol.32 No.3
Most of posttransfusion hepatitis will be developed by hepatitis C virus. So the ultimate goal of blood donor screening of anti HCV antibodies is specific exclusion of viral carrier from the blood donor population. To evaluate whether various enzymatic immunoassay for anti HCV antibodies are an useful methods to rule out viral carrier or not, the author investigated the sensitivity and specificity of various 2nd generation anti HCV reagent. There were noticed some false positive and false negative by different reagent and also demonstrated same results with control sample confirmed by RT-PCR. From these results it is suggested that 3rd generation anti HCV antibody should be adopted for screening of donor blood.
일부(一部) 농촌지역고혈압(農村地域高血壓) 환자(患者)에 대(對)한 위험요인(危險要因)으로서의 생화학적검사치(生化學的檢査値)에 관(關)한 연구(硏究)
장순모 ( Soon Mo Chang ),윤영옥 ( Young Ok Yoon ) 대한임상검사과학회 1984 대한임상검사과학회지(KJCLS) Vol.16 No.1
This study was carried out in Chun cheon City and Chunseong county in 1984, in order to identify the relationship between biochemical test values and hypertension. Biochemical test items were HDL-C, VLDL-C, total cholesterol , triglyceride and uric acid. The results obtained were as follows: 1. The mean values of this biochemical tests in normal control group were 46±13mg/dl for HDL-C, 25±16mg/dl for VLDL-C, 88±22mg/dl for LDL-C, 3.6±1.1 for T-Cholesterol/HDL-C Ratio, 2.1±1.0 for LDL-C/HDL-C ratio, 157±28mg/dl for T-Cholesterol, 150±76mg/dl for triglyceride, 4.1±1.4mg/dl for uric acid. 2. The mean values of the tests in hypertensive group were 40±10mg/dl for HDL-C, 30±18mg /dl for VLDL-C, 97±26mg/dl for LDL-C, 4.4±1.3 for T-Cholesterol/HDL-C ratio, 2.6±0.9 for LDL-C/HDL-C ratio, 168±32mg/dl for T-Cholesterol, 200±123mg/dl for triglyceride, 4.9±1.9 mg/dl for uric acid. 3. The values of HDL-C was lower significantly in hypertensive group than in control group. 4. The values of VLDL-C, LDL-C, T-Cholesterol/HDL-C ratio, LDL-C/HDL-C ratio, T-Cholesterol, T.G. and uric acid were higher significantly in hypertensive group than in contral group.