노인가구의 환경요인과 욕구특성에 관한 연구

임윤숙,김미자,이희진 전국상담복지학회 2018 전국상담복지연구 Vol.1 No.-

현대사회는 노인인구의 증가와 더불어 핵가족화가 가속화 되면서 노인세대를 독립된 세대로 점점 나누어 가고 있다. 이로 인해 노인가족 역시 증가추세를 보이고 있다. 베이비붐 세대는 지금까지 사회 전반적 흐름을 주도해온 세대로서 기존의 노인들과는 차이를 보인다. 기존의 노인층은 노년을 인생의 종말기로 인식하고 있지만, 베이비붐 세대는 이 시기를 건강하게 보내면서 자아실 현의 기회로 삼으려는 경향을 보이고 있다. 노인가구의 환경요인과 욕구특성을 분석하여 주택문제를 비롯하여 문화 활동 및 여가와 취미생활 등을 욕구특성과 환경요인에 맞도록 제안하여 베이비붐세대들이 노년을 행복하고 건강하게 보낼 수 있도록 하는 것이 연구의 목적이다. In modern society, as the elderly population increases, the nuclear family is accelerated, and the elderly households are gradually being divided into independent generations. As a result, the elderly family is also showing an increasing trend. The baby boom generation has been leading the society as a whole and differs from the old ones. The older elderly people perceive old age as the end of life, but the baby boomer generation tends to use this opportunity as an opportunity for self - realization. The analysis of the environmental factors and desire characteristics of the elderly households suggests that the baby boomers are happy and healthy by suggesting the housing problems, cultural activities, leisure and hobbies according to the desire characteristics and environmental factors. The purpose of.

신경계 조절에서 비타민의 역할

임윤숙 한국식품영양학회 2007 한국식품영양학회 학술대회논문집(학술심포지움) Vol.2007 No.12

Synthesis and Characterization of Water-Soluble and Self -Doped Conducting Poly(3,4-ethylenedioxythiiophene) Graft Copolymer

임윤숙,허준,조원호 한국고분자학회 2006 한국고분자학회 학술대회 연구논문 초록집 Vol.2006 No.4

Adenosylhomocysteinase like 1 interacts with nonstructural 5A and regulates hepatitis C virus propagation

임윤숙,Mai Han N.,Nguyen Lap P.,Kang Sang Min,Tark Dongseob,Hwang Soon B. 한국미생물학회 2021 The journal of microbiology Vol.59 No.1

Hepatitis C virus (HCV) life cycle is highly dependent on cellular proteins for viral propagation. In order to identify the cellular factors involved in HCV propagation, we previously performed a protein microarray assay using the HCV nonstructural 5A (NS5A) protein as a probe. Of ~9,000 human cellular proteins immobilized in a microarray, adenosylhomocysteinase like 1 (AHCYL1) was among 90 proteins identified as NS5A interactors. Of these candidates, AHCYL1 was selected for further study. In the present study, we verified the physical interaction between NS5A and AHCYL1 by both in vitro pulldown and coimmunoprecipitation assays. Furthermore, HCV NS5A interacted with endogenous AHCYL1 in Jc1-infected cells. Both NS5A and AHCYL1 were colocalized in the cytoplasmic region in HCV-replicating cells. siRNAmediated knockdown of AHCYL1 abrogated HCV propagation. Exogenous expression of the siRNA-resistant AHCYL1 mutant, but not of the wild-type AHCYL1, restored HCV protein expression levels, indicating that AHCYL1 was required specifically for HCV propagation. Importantly, AHCYL1 was involved in the HCV internal ribosome entry site-mediated translation step of the HCV life cycle. Finally, we demonstrated that the proteasomal degradation pathway of AHCYL1 was modulated by persistent HCV infection. Collectively, these data suggest that HCV may modulate the AHCYL1 protein to promote viral propagation.

Protein kinase Cμ plays an essential role in hypertonicity-induced heat shock protein 70 expression

임윤숙,Jae-Seon Lee,Tai-Qin Huang,서정선 생화학분자생물학회 2008 Experimental and molecular medicine Vol.40 No.6

Heat shock protein 70 (HSP70), which evidences important functions as a molecular chaperone and anti- apoptotic molecule, is substantially induced in cells exposed to a variety of stresses, including hypertonic stress, heavy metals, heat shock, and oxidative stress, and prevents cellular damage under these conditions. However, the molecular mechanism underlying the induction of HSP70 in response to hypertonicity has been characterized to a far lesser extent. In this study, we have investigated the cellular signaling pathway of HSP70 induction under hypertonic conditions. Initially, we applied a variety of kinase inhibitors to NIH3T3 cells that had been exposed to hypertonicity. The induction of HSP70 was suppressed specifically by treatment with protein kinase C (PKC) inhibitors (G̈ö6976 and GF109203X). As hypertonicity dramatically increased the phosphorylation of PKCμ, we then evaluated the role of PKCμ in hypertonicity-induced HSP70 expression and cell viability. The depletion of PKCμ with siRNA or the inhibition of PKCμ activity with inhibitors resulted in a reduction in HSP70 induction and cell viability. Tonicity-responsive enhancer binding protein (TonEBP), a transcription factor for hypertonicity- induced HSP70 expression, was translocated rapidly into the nucleus and was modified gradually in the nucleus under hypertonic conditions. When we administered treatment with PKC inhibitors, the mobility shift of TonEBP was affected in the nucleus. However, PKCμ evidenced no subcellular co-localization with TonEBP during hypertonic exposure. From our results, we have concluded that PKCμ performs a critical function in hypertonicity-induced HSP70 induction, and finally cellular protection, via the indirect regulation of TonEBP modification. Heat shock protein 70 (HSP70), which evidences important functions as a molecular chaperone and anti- apoptotic molecule, is substantially induced in cells exposed to a variety of stresses, including hypertonic stress, heavy metals, heat shock, and oxidative stress, and prevents cellular damage under these conditions. However, the molecular mechanism underlying the induction of HSP70 in response to hypertonicity has been characterized to a far lesser extent. In this study, we have investigated the cellular signaling pathway of HSP70 induction under hypertonic conditions. Initially, we applied a variety of kinase inhibitors to NIH3T3 cells that had been exposed to hypertonicity. The induction of HSP70 was suppressed specifically by treatment with protein kinase C (PKC) inhibitors (G̈ö6976 and GF109203X). As hypertonicity dramatically increased the phosphorylation of PKCμ, we then evaluated the role of PKCμ in hypertonicity-induced HSP70 expression and cell viability. The depletion of PKCμ with siRNA or the inhibition of PKCμ activity with inhibitors resulted in a reduction in HSP70 induction and cell viability. Tonicity-responsive enhancer binding protein (TonEBP), a transcription factor for hypertonicity- induced HSP70 expression, was translocated rapidly into the nucleus and was modified gradually in the nucleus under hypertonic conditions. When we administered treatment with PKC inhibitors, the mobility shift of TonEBP was affected in the nucleus. However, PKCμ evidenced no subcellular co-localization with TonEBP during hypertonic exposure. From our results, we have concluded that PKCμ performs a critical function in hypertonicity-induced HSP70 induction, and finally cellular protection, via the indirect regulation of TonEBP modification.

고농도의 감마 토코페롤 보충식이가 흡연에 노출된 쥐의 혈액 및 조직 비타민 E와 대사산물 농도에 미치는 영향

임윤숙(Lim Yunsook) 韓國營養學會 2008 Journal of Nutrition and Health Vol.41 No.2

흡연은 활성 산소/절소종의 생성을 증가시켜 체내 산화적 스트레스를 증가시키고 폐의 염증을 유발한다. 이는 흡연자들의 체내 항산화 영양소들의 감소와 밀접한 관계를 가지는 것으로 알려져 있다. 본 연구에서는 항산화, 항염증 기능을 가진 비타민 E 중 식이에 많이 포함되어 있는 GT를 이용하여 흡연에 의한 항산화, 항염증 작용을 알아보기 위한 선행 연구로 고농도의 GT 식이가 혈액과 간, 폐의 AT, GT농도와 이들의 대사산물인 CEHC 농도에 미치는 영향에 대하여 알아보고자 실시하였다. 연구 결과 고농도 GT 식이는 체내 혈액과 간, 폐 조직에 GT를 축적시키고 G-CEHC의 배설을 증가시켰다. 흡연은 대조군의 혈액과 폐 AT농도는 증가시켰지만, 고농도 GT 군의 혈액과 폐의 GT농도와 간의 G-CEHC의 농도를 감소 시켰다. 이러한 변화는 흡연에 의한 산화적 스트레스 상태에서 각기 다른 기능을 가진 조직의 요구량에 따라, 폐의 이용률을 높이기 위해 혈액으로 운반되는 양을 증가시키고 폐로의 운반을 일시적으로 증가시켜 조직의 AT, GT 농도를 선택적으로 조절하고 GT의 생체 이용률의 증가 때문이라 사료된다. 하지만 정확한 가전에 대한 연구들이 부족한 실정이므로 고농도의 GT 식이가 흡연에 의해 유도된 체내 산화적 스트레스와 폐의 염증 반응에 긍정적인 효과를 알아보기 위한 후속 연구가 절실히 필요한 것으로 사료된다. Cigarette smoke (CS) induces oxidative and nitrosative stress to the respiratory tract (RT) via both oxidants contained in CS and by CS-induced activation of RT inflammatory-immune pro-oxidant processes. CS exposure has been associated with reduced levels of plasma micronutrient antioxidants, in part due to an increased utilization and turnover of alpha-tocopherol (AT). It has been suggested that gamma-tocopherol (GT) may have an expanded spectrum of antioxidant activation compared to alpha-tocopherol (AT). In order to investigate effects of high GT supplementation as compared to AT, C57 BL/6 mice were fed control AT (35 ㎎/㎏ diet) or high GT (1,000 ㎎/㎏ diet) diet for 8-10 weeks and then exposed to 60 ㎎/㎥ CS, 6 hr/day for 3 days. AT and GT levels and their metabolites were measured at endpoints. High GT supplementation significantly reduced AT levels in plasma, liver and lung compared to AT. CS increased levels of AT and GT in plasma and lung of control AT group but decreased GT levels in lung of high GT supplemented group. Moreover, CS significantly decreased GT metabolite, gamma-CEHC. The results suggest that high GT supplementation have selective modulation of concentrations of vitamin E and its metabolite in plasma and lung but not in liver against in vivo CS exposure. (Korean J Nutr 2008; 41 (2): 135~140)

영양소와 면역능의 상관관계

김현숙,임윤숙 숙명여자대학교 건강ㆍ생활과학연구소 1996 生活科學硏究誌 Vol.11 No.2

Korean Red Ginseng Combined with Nattokinase Ameliorates Dyslipidemia and the Area of Aortic Plaques in High Cholesterol-diet Fed Rabbits

강신정,임윤숙,김애정 한국식품과학회 2014 Food Science and Biotechnology Vol.23 No.1

Male white rabbits were fed a high cholesteroldiet supplemented with red ginseng (RG) or red ginsengplus nattokinase (RGNK). RG and RGNK significantlyreduced increased serum triglycerides levels and aorticplaque area in a hypercholesterol diet fed rabbits. Moreover,only RGNK reduced hepatic cholesterol and cholesterylester transfer protein activity levels. Therefore, the presentstudy suggests RGNK might be a potential therapeuticapproach for atherogenesis.

Conjugated Linoleic Acid (CLA)가 피부 상처 치유 과정에 미치는 영향

박나영,임윤숙 한국지질동맥경화학회(구 한국지질학회) 2007 한국지질동맥경화학회 추계학술발표논문집 Vol.2007 No.-

맞벌이가정 여성의 일·가정 양립이 저출산에 미치는 영향

이지은,임윤숙,김수희 전국사회복지학회 2019 전국사회복지연구 Vol.13 No.-

현대사회 맞벌이부부 여성이 일․가정양립 으로 저 출산에 미치는 요인을 사회가 변화하고 있음에도 불구하고 가사와 양육에 대한 책임이 여성의 몫으로 노동을 병행하도록 하고 있다. 이러한 사회에서 맞벌이 부부가 맘 놓고 아이를 낳을 수 있도록 하고, 출산 가능한 부부는 사회에 이바지 함으로써 출산의지를 갖고 스스로 출산율을 높이는데 공헌하여야 하겠다. 저 출산 해결을 위한 연구는 주로 맞벌이 여성과 여성의 연령을 중심으로 하고 있으나 출산 계획 시 둘째자녀에 대해서는 여러 가지 여건을 고려하여 검토하고 부부간의 합리적인 출산을 하고 있는 것으로 나타났다. 즉, 맞벌이 가정에서도 삶의 질을 높이기 위한 방법으로 출산 계획 시 저 출산에 대한 자료가 없어 어려움이 있었으나, 이 연구를 통해 체계적인 제도와 정부의 지원으로 다양한 혜택을 누릴 수 있기를 바란다. Even though society is changing the factors that double-income women have on low birthrates due to work and family cultivation, responsibilities for housekeeping and child rearing are on women's shoulders, and labor are on the same side. In this society, working couples should be allowed to have children freely, and those who are able to give birth should contribute to the society, thus contributing to raising the birth rate on their own. The research on childbirth is mainly focused on the age of working women and women, but it is found that the second child is considered for various conditions in the birth plan and has reasonable childbirth between couples. In other words, there have been difficulties in planning childbirth as a way to improve the quality of life for working families, but we hope to enjoy various benefits with systematic system and government support.