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8주간의 밸런스 트레이닝이 뇌졸중 환자의 우울척도 및 세로토닌에 미치는 영향
임대열,백일훈,윤병곤,박찬호 한국웰니스학회 2012 한국웰니스학회지 Vol.7 No.3
본 연구는 만성기 뇌졸중 환자들을 대상으로 8주간 균형운동이 뇌졸중 후 우울증의 개선과 우울증과 관계있는 세로토닌에 긍정적인 영향을 미치는 가를 알아보기 위하여 연구의 목적에 부합되는 뇌졸중 환자 21명을 선별하여 무작위로 9명의 운동군과 12명의 대조군을 선정하여 매회 50분간, 주 3회, 8주간의 밸런스 트레이닝을 실시하였고 트레이닝 전과 후에 각 그룹의 Berk Depression Inventory(BDI)검사와, 세로토닌을 측정하여 다음과 같은 결과를 도출하였다. 8주간의 밸런스 트레이닝 후 BDI 검사의 점수는 대조군과 운동군 모두에서 낮아졌지만 운동군에서 유의하게 낮아져 밸런스 트레이닝이 뇌졸중 환자의 우울증상 개선에 효과가 있었으며 세로토닌은 대조군에서 낮아졌지만 운동군에서는 유의하게 높아져 밸런스 트레이닝이 뇌졸중 환자의 세로토닌을 증가시키는데 효과가 있었다. 이와 같은 결과는 결국 뇌졸중 후의 재활치료과정에서 물리치료와 병행하여 밸런스 트레이닝을 실시하는 것이 환자들의 우울증상 개선에 보다 효과적이라는 것을 나타낸 것이라 할 수 있다. 향후 연구에서는 우울검사 뿐만 아니라 보행과 같이 중앙 패턴 발생기를 이용한 반복적이고 지속적인 동작과 관련된 연구도 진행되어야 할 필요성을 가지고 있다. The objective of this study was to evaluate the group balance training on the depression and the changes of serum serotonin levels in people with chronic hemiparetic stoke. Twenty-one chronic stroke patients who were hopitalized in Busan D Medical Center were selected by the inclusion criteria and were randomly assigned to either a control group(n=9) or an experimental group(n=12). Data for the following outcome measure were obtained before and after exercise: Berk Depression Inventory(BDI), serum serotonin levels. The results of this study were as follows: 1) After the group balance training, serum serotonin levels were significantly increased in experimental group compared with the control group. 2) After the group balance training, depression were significantly decreased in experimental group compared with the control group. In summary, these findings could feasibly be a valuable data for improvement of the balance, depression. More future studies will be needed for the group balance training in people with chronic hemiparetic stoke.
8주간의 균형운동 프로그램이 뇌졸중 환자의 균형능력에 미치는 영향
임대열(Lim, Dae-Loul),백일훈(Baek, Il-Hoon),박찬호(Park, Chan-Ho) 한국체육과학회 2012 한국체육과학회지 Vol.21 No.1
The objective of this study was to evaluate the group balance training on the changes of balance performance in people with chronic hemiparetic stoke. Chronic stroke patients who were hospitalized in Busan D Medical Center were selected by the inclusion criteria and were randomly assigned to either a control group(n=9) or an experimental group(n=12). Date for the following outcome measures were obtained before and after exercise : Berg Balance Scale(BBS), Time “Up & GO” Test(TUG), BDI, and Myoeletrical activity. The results of this study were as follows: 1) After the group balance training, the BBS scores were not significantly improved in the experimental group compared with the control group while significant improvements in TUG scores did occur in the experimental group compared with the control group. 2) Muscle activities in external oblique, gluteus medius, vastus medialis and tibialis anterior were a significantly interaction between the experimental group and control group. From these results of this study, the group balance training for 8 weeks has an positive effect on the balance performance as well as muscle activities in people with chronic hemiparetic stoke.
정휘민,임대균,김민선,정규열,오민규 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.1
Most of the bacteria have been evolved to maximize growth rate with fast consumption of carbon sources from surroundings. However, fast growing phenotype featured overproduction of acetate. There exist several glucose uptake pathways in E. coli. As the glucose transporters were mutated, the growth rate and glucose uptake decreased but acetate overflow was relieved and improved biomass was achieved. Transcriptome analysis was implemented to investigate the change between wild type and mutant systematically. Various transcriptional and metabolic transition were identified in mutant including central metabolism, TCA cycle, alternative transporters, quorum sensing, chemotaxis, flagella synthesis and stress induced response. We attempted to construct robust host strain by introducing several value-added compound (EGFP, γ-aminobutyrate, lycopene) pathway to sugar transporter mutants. Eventually, the specific production yields of above compounds were improved in mutants.
우성화,임대균,석주연,오민규,정규열 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.1
Biosynthesis of isoprenoids via the DXP pathway requires equimolar glyceraldehyde 3-phosphate and pyruvate to divert carbon flux toward the products of interest. Here, we demonstrate that precursor balancing is one of the critical steps for the production of isoprenoids in E. coli. First, the implementation of the synthetic lycopene production pathway as a model system and the amplification of the native DXP pathway were accomplished using synthetic promoters and redesigned 5'-UTR. Next, fine-controlled precursor balancing was investigated by tuning phosphoenolpyruvate synthase (PpsA) or glyceraldehyde 3-phosphate dehydrogenase (GAPDH). The results showed that tuning-down of gapA improved the specific lycopene content by 46% compared to the overexpression of ppsA. The lycopene in the strains with down-regulated gapA increased by 96% compared to that in the parental strain. Our results indicate that gapA is the best target for precursor balancing to increase biosynthesis of isoprenoids.
Artificial lactam riboswitch for high-throughput screening and selection
장성연,장성호,임대균,강택진,오민규,정규열 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.0
Caprolactam is a monomer used for the synthesis of nylon-6. An intracellular biosensor for caprolactam can facilitate high-throughput metabolic engineering of recombinant microbial strains. Because of the mixed production of caprolactam and valerolactam in the recombinant strain, a caprolactam biosensor should be highly specific for caprolactam. However, a highly specific caprolactam sensor has not been reported. Here, we developed an artificial riboswitch that specifically responds to caprolactam. This riboswitch was prepared using a coupled in vitro-in vivo selection strategy with a heterogeneous pool of RNA aptamers obtained from in vitro selection to construct a riboswitch library used in in vivo selection. The caprolactam riboswitch successfully discriminated caprolactam from valerolactam. This riboswitch enabled caprolactam-dependent control of cell growth, which will be useful for improving caprolactam production and is a valuable tool for metabolic engineering.
Characteristics and applications of sugar transporter mutated Escherichia coli
조희근,정휘민,임대균,정규열,오민규 한국공업화학회 2020 한국공업화학회 연구논문 초록집 Vol.2020 No.-
Most bacteria have evolved to maximize growth rate, with rapid consumption of carbon sources. However, fast growth feature secretion of organic acid or ethanol and reduce biomass yield in aerobic conditions. Several glucose uptake pathways exist in Escherichia coli, and when these transporters or their subunits were knocked out, the growth and glucose uptake rates decreased but acetate overflow was relieved and biomass yield was improved. Alteration of metabolism caused by the mutations was investigated with transcriptome analysis and <sup>13</sup>C metabolic flux analysis. As a result, various perturbations were identified in the sugar transporter mutants. Based on these findings, robust production hosts were constructed. When pathway genes for producing value-added compounds were introduced, the specific yields of the compounds were improved significantly in the sugar transporter mutants, suggesting that controlling the sugar uptake rate is a good strategy for improving metabolite production.
Development of RNA-based caprolactam sensor for high-throughput screening and selection
김민선,장성연,장성호,임대균,강택진,오민규,정규열 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.1
The Microbial strain which produces caprolactam, a monomer widely used for the synthesis of nylon-6, has been recently reported. One of the hurdles for screening high-producer is that the recombinant strain coproduce caprolactam and valerolactam which differs by only a single carbon. Therefore, it is pivotal for high-throughput screening to specifically report intracellular concentration of caprolactam. However, a highly specific caprolactam sensor has not been reported. Here, we developed an artificial riboswitch that specifically responds to the caprolactam using a coupled in vitro-in vivo selection strategy. Heterogeneous pool of RNA aptamers obtained from SELEX is inserted between ORF of reporting gene in a plasmid to proceed in vivo selection. Constructed caprolactam sensor was activated by 3.36-fold in the presence of 50 mM caprolactam and enabled caprolactamdependent control of cell growth, which will be useful for improving bio-based manufacture and metabolic engineering.
조민지,노명현,강채원,임대균,오민규,정규열 한국공업화학회 2020 한국공업화학회 연구논문 초록집 Vol.2020 No.-
L-tyrosine is a commercially important amino acid in industry. We attempt to control translation-level expression and carbon flux rebalancing around phosphoenolpyruvate (PEP) node. To optimize the L-tyrosine biosynthetic pathway, a synthetic constitutive promoter and a synthetic 5′-untranslated region (5′-UTR) were introduced for each gene of interest to allow for control at both transcription and translation levels. Carbon flux rebalancing was achieved by controlling the expression level of PEP synthetase using UTR Designer. The L-tyrosine productivity of the engineered E. coli strain was increased through pathway optimization resulting in 3.0g/L of L-tyrosine titer, 0.0354g L-tyrosine/h/g DCW of productivity, and 0.102g L-tyrosine/g glucose yield. Thus, this work demonstrates that pathway optimization by 5′ -UTR redesign is an effective strategy for the development of L-tyrosine-producing bacteria.
Precise control of glyoxylate cycle for production of tyrosine from acetate in Escherichia coli.
조민지,노명현,임현규,강채원,임대균,오민규,정규열 한국공업화학회 2019 한국공업화학회 연구논문 초록집 Vol.2019 No.1
Acetate is one of promising feedstocks owing to its cheap and great abundance. Considering that tyrosine production is shifting to microbial production method, its production from acetate can be attempted to improve the economic feasibility. We engineered a previously reported strain, SCK1, for efficient production of tyrosine from acetate. Initially, the acetate uptake and gluconeogenic pathway were amplified to maximize the flux toward tyrosine. As flux redistribution between glyoxylate and TCA cycles is critical for efficient precursor supplementation, the activity of the glyoxylate cycle was controlled by varied expression of isocitrate lyase. Consequently, the engineered strain with optimal flux distribution produced 0.70 g/L tyrosine with 20% of the theoretical maximum yield. This is the first demonstration of tyrosine production from acetate and our strategies would be widely applicable to the production of various chemicals from acetate.