비가족성 산발성 결직장암에서 Microsatellite Instability 와 병리조직학적 특징 및 p53 단백질 발현과의 연관성

양영일,김미성,윤혜경,임인숙 인제대학교 1997 仁濟醫學 Vol.18 No.4

Microsatellite instability는 선천성 비용종성 결직장암의 분자생물학적 원인 인자로 규명되었으며 산발성 결직장암에서도 원인 인자로 그 중요성이 제시되었다. 가족력이 동반되지 않은 산발성 결직장암 49예를 대상으로 Microsatellite instability의 빈도를 조사하여 병리학적 특성, 세포 증식력, p53 단백질 발현과의 연관성을 연구하였다. 비가족성 산발성 결직장암에서 Microsatellite instability는 24.5%에서 검출되었고, Microsatellite instability 양성 결직장암은 점액성 분화, 종양 주위의 기질내에 림프구 집합의 형성이 빈번히 동반되는 병리조직학적 특성들을 보였다. p53 단백질 발현은 microsatellite instability 양성 결직장암에서 양성율이 16.7%인 반면 음성인 종양인 경우는 67.6%로 p53 단백질 발현과 microsatellite instability 사이에는 의의 있는 역상관 관계를 보였다. 이상의 결과로 Microsatellite instability는 결직장암의 발생 과정 및 형태학적 형성 과정에 중요한 역할을 담당할 것으로 사료된다. Microsatellites are highly polymorphic, short-tandem repeat sequences dispersed throughout the genome. Defects in DNA mismatch repair can result in an increase in DNA replication errors (RER) or microsatellite instability (MSI), which are manifested as extra, aberrant bands within multiple microsatellite loci. MSI are found in the cancers from patients with hereditary non-polyposis colorectal cancer and have been also demonstrated in sporadic colorectal carcinomas (CRC). It has been suggested that the histologic features and molecular characteristics of MSI-positive CRC are similar to those of HNPCC. In order to clarify the relationship between MSI and pathological parameters including histologic features, proliferating activity, p53 protein expression, PCNA proliferating activity and PCR for microsatellite instability was performed on a series of 49 formalin-fixed CRC and normal mucosa. (1) MSI-positive CRC's were noted in 12 (24.5%) CRC cases, but no MSI was detected in normal mucosal tissues. (2) MSI-positive CRC's were closed related with extracellular mucin production, plexiform pattern, and Crohns-like lymphoid reaction (p<0.05). (3) No significant relationship between MSI and age, sex, tumor size, location, grade, stage, vascular invasion and PCNA index was noted (p<0.05). (4) p53 protein expression rate of 49 CRC cases was 55.1%, and p53 expression rate of MSI-negative group was 67.6% and the rate in MSI-positive group was 16.7%. The inverse relationship between MSI and p53 expression was statistically significant (p<0.05). In summary, microsatellite instability may play an important role in colorectal carcinogenesis and morphogenesis of non-familial colorectal carcinomas, and inverse correlation between MSI phenotype and p53 expression suggests MSI and p53 mutations are independent pathway in colorectal carcinogenesis. And MSI-associated unique pathologic features may be useful for screening criteria of patients wish HNPCC.

심실중격결손증-치험 1예-

조광현,우종수,황윤호,이양행,박철호,김종성,김철호,이경순,조영일,박태인 인제대학교 1985 仁濟醫學 Vol.6 No.4

A successful treatment of infants and children with congenital heart defects depends upon several factors: diagnosis must be accurate, the operative technique, including techniques of perfusion and myocardial preservation, must be suited to the needs of patients and intensive cares, including accurate anesthesia, must be applied through all stages of investigation and treatment, i.e. before, during and after the operation. Recently we performed a open heart surgery successfully for a six-years old girl who had suffered from frequent upper respiratory tract infection and mild to moderate exertional dyspnea with congenital heart defects (ventricular septal defect, Kirklin type II, with patent foramen ovale). The operation and postoperative course were very smooth. And now we report this with review of literatures.

胃腸管의 多發性線癌을 同伴한 肺尖部肺癌 一例 報告

朴聖信,金東洵,金禮會,徐賢淑,高一香 인제대학교 1985 仁濟醫學 Vol.6 No.4

Gastrointestinal metastasis of the lung cancer is an unusual finding but is found in 1-4% of the patients at the autopsy. Recently, several cases who were diagnosed as gastric metastasis during life were reported. We experienced a case of Pancoast type lung cancer (adenocarcinoma) and multiple adenocarcinomatous lesion in the gastrointestinal tract. Patient was a 39-year-old man who presented with left upper chest and arm pain. Chest x-ray showed a mass in the left apex. Aspiration cytology revealed a poorly differentiated adenocarcinoma. During a course of radiotherapy, he developed severe anemia. Gastroscopy revealed multiple fungating masses with umbilication in the esophagus, stomach and duodenum.

과립세포종 1예

김방순,박상만,고우석,박진남 인제대학교 1996 仁濟醫學 Vol.17 No.4

과립세포종은 주로 성인의 피부, 피하지방, 구강에 호발하며 조직학적 소견상 풍부한 호산성의 과립을 함유한 다각형 혹은 타원형의 중앙세포들이 군집을 이루는 양성종양으로 슈반세포(Schwann cell) 기원설이 유력하다. 저자들은 57세 남자의 두피에 발생한 과립세포종 1예를 경험하고 문헌고찰과 함께 보고하는 바이다. Granular cell tumors were originally described in 1926 by Abrikossoff as myoblastic myoma. They usually occur as solitary tumor but can be multiple in about 10% of cases. They have a predilection for the skin, subcutaneous tissue, and tongue but also occur in other organs. We report a case of solitary granular cell tumor in a 57-year-old male. He had a tender, 1.2 cm sized nodule on the scalp. The histopathologic findings revealed a distictive picture of granular cell tumor and S-100 protein, vimentin immunoreactivity.

전종뇌 1예

최종현,이응수,이홍균 인제대학교 1988 仁濟醫學 Vol.9 No.4

A case of holoprosencephaly associated with hydramnios is presented with a brief review of its literatures, This lobal type holoprosencephaly has following anomalies of fused thalamus and agenesis of corpus callosum, dorsal cyst, microphthalmia, one nostril and undetectable pituitary gland.

폐기물의 흡착제화와 건자재로의 활용

박흥재 인제대학교 낙동강환경연구소 2001 심포지움(인제대학교 부설 환경연구소 발표논문집) Vol.- No.10

중간세공 분자체 MCM-41의 세공표면에 Ni(Ⅱ) 혼입에 관한 광음향 분광학적 연구

박동호,박성수 인제대학교 1999 仁濟論叢 Vol.14 No.3

초산 니켈(Ⅱ) 수용액과 염산 및 인산을 사용하여 pH=2.5로 조절한 초산 니켈(Ⅱ) 수용액을 가지고, 적심 방법을 이용하여 ㆍ MCM-41에 Ni(Ⅱ)를 혼입하였다. 그리고 공기 중에서 하소시켜 각각 Ni/MCM41, Ni-Cl/MCM41 그리고 Ni-PO/MCM41을 제조하였다. MCM-41 세공 내에 혼입된 Ni(Ⅱ)의 존재형태는 광음향 분광법(PAS)을 이용하여 조사하였다. Ni/MCM41, Ni-Cl/MCM41 그리고 Ni-PO/MCM41은 하소 이전의 광음향 스펙트럼에서 400 nm 부근과 750 nm 부근에서 최대 흡수밴드를 보였다. 이 두 흡수밴드는 초산이온, 염소 이온, 물분자 또는 인산이온 등에 의해 팔면체 구조의 환경에 있는 Ni(II)의 3A2g→3T1g(F) 그리고 3A2g→3T1g(P) 전이 때문이다. Ni/MCM41과 Ni-PO/MCM41은 하소 후 400 nm 부근의 밴드는 약 35 nm가 장파장 쪽으로 이동하였다. 그리고 500 nm~630 nm에서 두 개의 shoulder가 보인다. 이는 Ni(II)이 뒤틀린 팔면체 구조 환경 속에 있음을 보여준다. Ni-Cl/MCM41은 407 nm와 712 nm 부근에서 두 개의 흡수밴드를 보인다. 그리고 500nm∼600 nm에서 두 개의 shoulder가 보인다. 인산 리간드를 가지고 있는 Ni-PO/MCM41은 Ni/MCM41과 Ni-Cl/MCM41 경우와 달리 Ni(II) 종이 세공표면의 실란올기와 반응함에 따라 뭉치산화물 형태로 존재하지 않고, 팔면체 구조를 이루며 MCM-41 세공표면에 원자적으로 잘 분산되어있다. 중심 단어 : 중간세공 분자체 MCM-41, 광음향 분광법, 니켈 팔면체 종, 인산 리간드 The incorporation of Ni(II) within mesoporous molecular sieve MCM-41 by the incipient wetness method using Ni(II) aqueous solution at pH=2.5 adjusted with hydrochlroric acid or phosphoric acid (Ni/MCM41, Ni-Cl/MCM41 and Ni-PO/MCM41, respectively.) has been investigated. Photoacoustic spectroscopy (PAS) technique was applied to study the local environments of Ni(II) incorporated within MCM-41 channel. The PAS of as-prepared Ni/MCM41, Ni-Cl/MCM41, Ni-PO/MCM41, respectively, with Ni(II) acetate aqueous solution exhibits two bands of around λmax=400 nm and 750 nm, which could be assigned to the 3A2g→3T1g(F) and the 3A2g→3T1g(P) transition of Ni(II) surrounded octahedrally by acetate ions chloride ions, water molecules, or phosphate ions. After calcined, Ni(II) spectral bands of Ni/MCM41 and Ni-PO/MCM41 at around 400 nm were red-shifted by about 35 nm, and exhibits two shoulders at 500 nm ∼630 nm were appeared. It may attributed to the distorted octahedral symmetry of Ni(II) within MCM-41. In case of Ni-PO/MCM41, the octahedral Ni species containing phosphate ligands in coordination sphere react with framework's silanol groups to be dispersed atomically onto the surface of MCM-41 channel as a octahedral species without the transformation to Ni oxide phase. The Ni species within Ni-Cl/MCM41 and Ni/MCM41, on the other hand, transform to Ni oxide phase upon calcination. Key words : mesoporous molecular sieve MCM-41, Photoacoustic spectroscopy(PAS), octahedral Ni species, phosphate ligands.

암 치료의 최신 동향 : 두경부암

김상효 인제대학교 1984 仁濟醫學 Vol.5 No.3

연속주조된 Cu-42wt%Zn 합금에서 미량원소첨가가 기계적 특성에 미치는 영향 (초)

전성민,조건상,이광엽,박원욱,인제대 대한금속ㆍ재료학회 2009 대한금속재료학회 학술대회 개요집 Vol.2009 No.-