한국 남성 불임환자에서 Y 염색체상의 AZF Gene에 대한 분석 및 DAZ Gene의 발현 양상

이호준,이형송,송견지,변혜경,서주태,김종현,이유식,Lee, Ho-Joon,Lee, Hyoung-Song,Song, Gyun-Jee,Byun, Hye-Kyung,Seo, Ju-Tae,Kim, Jong-Hyun,Lee, You-Sik 대한생식의학회 1997 Clinical and Experimental Reproductive Medicine Vol.24 No.1

Cytogenetic observations of loss of the distal portion of the Y chromosome long arm were found to be associated with disrupted spermatogenesis. The existence of a gene involved in the regulation of spermatogenesis, the azoospermia factor (AZF), was postulated. In this study, we screened the AZF region including DAZ and DAZH genes and observed the expression pattern of DAZ and DAZH transcript in infertile men with azoospermia and oligospermia by using a sequence-tagged site (STS)-based PCR method. PCR primers were synthesized for 11 STSs that span Yq interval 6, SRY, DAZ, and DAZH, functional DAZ homologue on chromosome 3. Microdeletions were detected in 4/32 (12.5%) azoospermic men and 1/11 (9%) severe oligospermic men. Only 2 of 5 patients had microdeletions of Yq that contained the DAZ gene, whereas the other 3 patients had deletions extending from intervals 5L-6F proximal to the DAZ gene on Yq. Testis biopsies of the azoospermic patients revealed a variety from Sertoli cell-only syndrome to testicular maturation arrest. Of 4 men with clinical data available, average testis size was R: 13.8 cc, L: 13.8 cc, serum T was $4.0{\pm}1.25$ ng/ml, LH was $3.63{\pm}1.90$ mIU/ml, and FSH was $8.85{\pm}5.13$ mIU/ml. These values did not differ significantly from the remainder of the patients tested. We could not observed the DAZ transcript in 2 patients, who have no mature spermatozoa. In 11.6% of patients microdeletions of the AZF could be detected. These deletions in the AZF region seem to be involved causing spermatogenic failure. But the frequency of microdeletions proximal to DAZ suggests that DAZ is not the only gene associated with spermatogenic failure.

초기 비소세포 폐암 환자의 늑막하 병변이 예후에 미치는 영향; 예비 보고

이호준,이형식,허원주,이기남,최필조,Lee, Ho-Jun,Lee, Hyung-Sik,Hur, Won-Joo,Lee, Ki-Nam,Choi, Pill-Jo 대한방사선종양학회 1998 Radiation Oncology Journal Vol.16 No.4

Purpose : We retrospectively analyzed the impact of subpleural lesions of early stage non-small cell lung cancer on the patterns of failure to support selection of postoperative adjuvant therapy. Methods and Materials : The study included 91 patients who underwent surgery for early stage non-small cell lung cancer at Dong-A University Hospital from Dec 1990 to Sep 1996. Twenty five patients were excluded due to postoperative mortality (four patients, 4.4$\%$) and stage III (21 patients). Of 66 patients, 22 patients were subpleural lesions (15 patients in stage I, and seven patients in stage II). Postoperative adjuvant radiation therapy was given to seven patients with T2Nl disease. The median follow-up duration was 29.5 months (range; 8-84 months). Results : The overall survival rate was 69.5$\%$ at 3 years. For all patients who presented with (22 patients) and without (44 patients) subpleural lesions, 3-year overall survival rates were 35.5$\%$ and 84.6$\%$, respectively (p=0.0017). For stage I patients who presented with (15 patients) and without (29 patients) subpleural lesions, 3-year overall survival rates were 33.1$\%$ and 92.3$\%$, respectively (p=0.001). For stage II patients who presented with (7 patients) and without (15 patients) subpleural lesions, 3-year overall survival rates were 53.3$\%$ and 45.7$\%$, respectively (p=0.911). For patients with T2N0 disease (34 patients) who presented with (11 patients) and without (23 patients) subpleural lesions, 3-year overall survival rates were 27.3$\%$ and 90.3$\%$, respectively (p=0.009). Conclusion : These observations suggest that the subpleural lesion play an important role as a prognostic factor for early stage non-small cell lung cancer. Especially for T2N0 disease, patients with subpleural lesions showed significantly lower survival rate than those without that. 목적 : 초기 비소세포 폐암환자의 치료시 늑막하병변이 있는 경우에 치료후 결과에 어떤 영향을 주는지를 분석하고, 이 결과를 토대로 향후 치료방침의 결정에 도움을 주기 위해서 본 연구를 하였다. 대상 및 방법 : 1990년 12월부터 1996년 9월까지 동아대학병원에서 절제가능한 비소세포 폐암으로 진단받고 수술을 받은 환자 91명중 제 I병기와 제 II병기환자 66명을 대상으로 후향적 분석을 하였다. 66명중 늑막하병변이 있는 경우는 22명이었으며, 제 I병기(T1N0, T2N0)에서 늑막하병변이 있는 경우는 15명/44명(34.1$\%$)이었으며, 제 II병기(T1N1, T2Nl, T3No)에서 늑막하병변이 있는 경우는 7명/22명(31.8$\%$)이었다. 수술후 보조요법으로서 전신 항암요법은 시행되지 않았고, 방사선치료는 7명에서 시행되었다. 최소 추적관찰기간은 8개월이며 최대추적관찰기간은 84개월이며, 평균 추적관찰 기간은 33.2개월(중앙값; 29.5개월)이었다. 생존율은 Kaplan-Meier법으로 분석하였고, 서로간의 성적비교는 log-lank test를 이용하였다. 결과 : 전체환자의 3년 생존율은 69.5$\%$였다. 전체환자에서 늑막하병변이 있는 경우와 없는 경우의 3년 생존율은 각각 35.5$\%$와 84.6$\%$였으며, 통계학적으로 의미있는 차이를 보였다(p=0.0017). 제 I병기에서 늑막하병변이 있는 경우와 없는 경우의 3년 생존율은 각각 33.1$\%$와 92.3$\%$였으며, 통계학적으로 의미있는 차이를 보였다(p=0.001). 제 II병기에서 늑막하병변이 있는 경우와 없는 경우의 3년 생존율은 각각 53.3$\%$와 45.7$\%$였으며, 통계학적으로 차이는 없었다 (p=0.911). 제 I병기환자 44명중 원발종양의 크기가 3cm이상인 경우(T2, 34명)는 늑막하병변이 있는 경우와 없는 경우의 3년 생존율은 각각 27.3$\%$와 90.3$\%$였으며, 통계학적으로 의미있는 차이를 보였다(p=0.009). 국소재발은 늑막하병변이 있는 경우에는 제 I병기에서 2명/15명(13.3$\%$), 제 II병기에서 2명/7명(28.6$\%$)에서 재발하였고, 늑막하병변이 없는 경우에는 제 I병기에서 4명/29명(13.8$\%$), 제 II병기에서 2명/14명(14.3$\%$)에서 국소재발을 보였다. 원격전이는 늑막하병변이 있는 경우에는 제 I병기에서 5명/15명(33.3$\%$), 제 II병기에서 2명/7명(28.6$\%$)에서 원격전이를 보였고, 늑막하병변이 없는 경우에는 제 1병기에서 2명/29명(6.9$\%$), 제 II병기에서 4명/14명(28.6$\%$)에서 원격전이를 보였다. 결론 : 초기 비소세포 폐암환자의 예후에 있어서 늑막하병변의 유무가 중요한 역할을 하는 것으로 생각되며, 특히 임파선 전이가 없는 72병기의 경우 늑막하병변이 있으면 늑막하병변이 없는 경우에 비해 생존율에서 현저한 차이를 보였다.

Miscanthus sinensis 와 타 식물과의 친근성에 관한 연구

정일구(Il Koo Lee),이호준(Ho Joon Lee),이경순(Kyung Soon Lee) 한국식물학회 1970 Journal of Plant Biology Vol.13 No.4

Althought allelopathy has been cleary established for Pinus densiflora by Lee-Monsi, the auther suspected that Miscanthus sinensis which grows vigrously in such Korean pine forests might also exhibit the phenomenon. A total of 33 species having an affinity in the Miscanthus group were recorded in the field. Out of these, five species; Patrinia scabiosaefolia, Lespedeza crytobotrya. Oenothera odorata, Raphanus sativus var. acanthiformis, and Zoysia japonica were considered the A group. Five other species which have no affinity to Miscanthus sinensis, Amaranthus patulus, Solanum nigrum, Capsella bursa-pastorris var. triangularis, Chemopodium album var. centrorubrum and Alopeculus amurensis were considered the B group. Extracts of Miscanthus sinensis were applied to determine relative germination and growth. It was found the growth in the B group was promoted by a cold water extract of Miscanthus stems and leaves, but inhibited remarkably by an extract obtained by passing cold water through Miscanthus root. As expected, growth in the A group was promoted by both experimental treatments.

프리캐스트 콘크리트에 의해 피복된 콘크리트충전 강관기둥의 편심압축실험

이호준,박홍근,김성배,박성순,Lee, Ho Jun,Park, Hong Gun,Kim, Sung Bae,Park, Sung Soon 한국강구조학회 2014 韓國鋼構造學會 論文集 Vol.26 No.1

본 연구에서는 콘크리트충전 각형강관에 구조용 피복콘크리트를 적용한 합성기둥에 대하여 연구하였다. 1/3~1/2 축소모델의 편심 압축실험체 4개와 중심압축실험체 1개를 제작하여 압축실험을 수행하였다. 실험 변수는 피복콘크리트의 강섬유 첨가여부, 편심거리, 기둥 길이, 그리고 횡보강 상세이다. 일부 실험체에서 최대강도 도달 후 피복콘크리트의 탈락으로 인한 강도저하가 발생하였으나 모든 실험체는 현행 설계기준(KBC 2009)에 의한 휨 압축 강도 및 공칭 압축강도를 초과하는 하중재하능력을 보였다. In this paper, concrete-filled steel tubular columns encased with precast reinforced concrete were studied. Four eccentrically loaded columns and a concentrically loaded column were tested to investigate the axial load-carrying capacity. The test parameters were the use of fiber reinforcement for cover concrete, eccentricity, column length, and lateral reinforcement. The maximum axial loads of the specimens agreed with the nominal strengths predicted by KBC 2009. However, in some specimens, the load carrying capacity quickly decreased after the peak strength due to spalling of the cover concrete.

자율주행을 위한 레이더 기반 인지 알고리즘의 정량적 분석

이호준,채흥석,서호태,이경수,Lee, Hojoon,Chae, HeungSeok,Seo, Hotae,Yi, Kyongsu 한국자동차안전학회 2018 자동차안전학회지 Vol.10 No.2

This paper presents a quantitative evaluation method and result of moving vehicle perception using automotive radar. It is also important to analyze the accuracy of the perception algorithm quantitatively as well as to accurately percept nearby moving vehicles for safe and efficient autonomous driving. In this study, accuracy of the automotive radar-based perception algorithm which is developed based on interacting multiple model (IMM) has been verified via vehicle tests on real roads. In order to obtain experimental data for quantitative evaluation, Long Range Radar (LRR) has been mounted on the front of the ego vehicle and Short Range Radar (SRR) has been mounted on the rear side of both sides. RT-range has been installed on the ego vehicle and the target vehicle to simultaneously collect reference data on the states of the two vehicles. The experimental data is acquired in various relative positions and velocity, and the accuracy of the algorithm has been analyzed according to relative position and velocity. Quantitative analysis is conducted on relative position, relative heading angle, absolute velocity, and yaw rate of each vehicle.

기업 내 학력의 외부효과와 학습조직의 관계 분석

이호준,이성은,정승환,정설미,정동욱,Lee. Ho Jun,Lee. Seong Eun,Jeong. Seung Hwan,Jeong. Seol Mi,Jeong. Dong Wook 한국교육재정경제학회 2015 敎育財政 經濟硏究 Vol.24 No.2

본 연구는 인적자본기업패널의 2~5차년도 자료를 활용하여 기업 내에서 학력의 외부효과가 존재하는지를 분석하고 학력의 외부효과와 학습조직의 관계 를 탐색하는데 연구의 목적이 있다. 또한, 본 연구는 학습조직을 7가지 하위요 소로 분류하고 각 하위요소와 외부효과 간의 관련성을 분석함으로써 외부효과 와 밀접한 관련이 있는 학습조직의 특성을 도출하여 학력의 외부효과를 제고 하기 위한 정책적 시사점을 논의하고자 하였다. 이를 위해 본 연구는 관찰하기 어려운 개별 기업의 이질적인 특성과 특정 시점의 고정적인 특성을 동시에 통 제할 수 있는 이원고정효과모형(Two-way Fixed Effect Model)을 적용하였다. 분석 결과, 기업 내 학력의 긍정적인 외부효과가 존재하는 것으로 나타났으 며, 학습조직의 성향이 강할수록 기업 내 학력의 외부효과가 더욱 증대됨을 확인하였다. 특히, 기업 내 팀원에게 많은 권한이 부여되는 조직문화를 가질수 록, 기업 내에서 구성원 간 의사소통이 활발할수록, 학력의 외부효과가 더 커 지는 것으로 나타났다. 이런 결과는 학력의 외부효과를 촉진시키기 위해서는 학습조직으로 조직을 변화시키려는 노력이 필요하며 구성원에게 더 많은 자율 권을 위임하고 구성원 간의 소통을 강화하려는 노력이 필요함을 말해 준다. 이런 결과를 바탕으로 본 연구는 교육정책이나 교육프로그램의 효과를 평가하는 과정에서 비수혜자들에게 미친 외부효과까지도 충분히 고려될 필요가 있으며 외부효과를 높이기 위해서 학습조직의 특성을 가진 조직 문화로 변화 시키려는 노력이 필요함을 정책적 시사점으로 제시하는 바이다. The purpose of this article is to empirically examine the existence of spillover effect from peers in companies, and to investigate the relationship between the spillover effect and learning organization. Furthermore, we analyze what characteristics of learning organization are critical to make differences of the spillover effect. For resolving endogenous problems which may occur by unobserved heterogenous company features and time specific effect, we implement two-way fixed effect model. As results, we found that there were positive spillover effect, which meas that the more educated peers are in organization the more one's productivity improve. Moreover, our results indicates that this positive spillover effect is more likely to be reinforced in learning organization. It is also required to highlight that the spillover effect may be facilitated in organizations where more inclined to empower decision-making authority from top to bottom for improving local knowledge. Furthermore, it is noteworthy that the more communication with colleagues seems to enhance spillover effect in a organization. These results, foremost, imply that it is imperative to re-examine the effect of education or education programs which are targeted to a particular group. For more comprehensive understanding about the effect of education, we recommend that the spillover effect should be considered as an unintended effect. Our result that the organization culture as a learning organization may reinforce the spillover effect suggest changing organization culture into learning organization may improve productivity of the organization.

체외배양 생쥐정소세포에서 합성에스트로겐이 P450 등위효소의 발현에 미치는 영향

이호준,김묘경,고덕성,김길수,강희규,김동훈,Lee, Ho-Joon,Kim, Myo-Kyung,Ko, Duck-Sung,Kim, Kil-Soo,Kang, Hee-Kyoo,Kim, Dong-Hoon 대한생식의학회 2001 Clinical and Experimental Reproductive Medicine Vol.28 No.2

Objective: To know the effects of xenoestrogen on spermatogenesis, we investigated the expression of cytochrome P450s enzymes (CYPscc, $CYP_{17{\alpha}}$, CYP19) and $3{\beta}$-HSD genes involved in steroidogenesis. Methods: Mouse testicular cells were prepared from 15-day-old ICR mice which had only pre-meiotic germ cells by enzyme digestion using collagenase and trypsin. Testicular cells were cultured in DMEM supplemented with FSH (0.1 IU/ml) and 10% FBS or medium with estrogen ($E_2$), bisphenol-A (BPA), octylphenol (OP; $10^{-9},\;10^{-7},\;10^{-6},\;10^{-5},\;10^{-4}M$, respectively) and aroclor 1254 (A1254) known as PCBs for 48 hours. The gene expression of cytochrome P450 enzymes were examined by semi-quantitive RT-PCR. The production of estrogen and testosterone was examined by RIA. Results: As results, expression of CYPscc mRNA was not significantly decreased, but $3{\beta}$-HSD and $CYP_{17{\alpha}}$. mRNA were significantly dose-dependent decreased. And production of testosterone and estrogen were not different except BPA and OP group ($10^{-5}M$). Conclusion: BPA, OP and A1254 might inhibit steroidogenesis by decreasing CYPscc, $3{\beta}$-HSD and $CYP_{17{\alpha}}$. mRNA expression in the mouse testis. These results suggest that BPA, OP and PCBs like as an endocrine disruptors inhibit the productions of steroidogenic enzymes and decrease the production of T and E by negative feedback mechanism. Therefore, these might disrupt steroidogenesis in Leydig cells of testis and would disturb testicular function and subsequently impair spermatogenesis.

차세대 2차원 소재, MXenes의 연구 동향

이호준,윤예준,장진광,변종민,Lee, Hojun,Yun, Yejun,Jang, Jinkwang,Byun, Jongmin 한국분말재료학회 (*구 분말야금학회) 2021 한국분말재료학회지 (KPMI) Vol.28 No.2

Interest in eco-friendly materials with high efficiencies is increasing significantly as science and technology undergo a paradigm shift toward environment-friendly and sustainable development. MXenes, a class of two-dimensional inorganic compounds, are generally defined as transition metal carbides or nitrides composed of few-atoms-thick layers with functional groups. Recently MXenes, because of their desirable electrical, thermal, and mechanical properties that emerge from conductive layered structures with tunable surface terminations, have garnered significant attention as promising candidates for energy storage applications (e.g., supercapacitors and electrode materials for Li-ion batteries), water purification, and gas sensors. In this review, we introduce MXenes and describe their properties and research trends by classifying them into two main categories: transition metal carbides and nitrides, including Ti-based MXenes, Mo-based MXenes, and Nb-based MXenes.

원통형 밀폐공간 내부의 능동소음제어

이호준,박현철,황운봉,Lee, Ho-Jun,Park, Hyeon-Cheol,Hwang, Un-Bong 대한기계학회 2000 大韓機械學會論文集A Vol.24 No.9

An active control of the transmission of noise through an aircraft fuselage is investigated numerically. A cylinder-cavity system was used as a model for this study. The fuselage is modeled as a fi nite, thin shel cylinder with constant thickness. The sound field generated by an exterior monopole source is transmitted into the cavity through the cylinder. Point force actuators on the cylinder are driven by error sensor that is placed in 3D cavity. Modal coupling theory is used to formulate the numerical models and describe the system behavior. Minimization of the acoustic potential energy in the fuselage is carried out as a performance index. Continuous parameter genetic algorithm is used to search the optimal actuator position and both results are compared.

${\mu}-PD$ 법으로 성장시킨 near-stoichiometric 조성 $Zn:LiNbO_3$ fiber 단결정 성장 및 광손상 특성

이호준,서중원,신동익,송원영,윤대호,Lee, H.J.,Shur, J.W.,Shin, T.I.,Song, W.Y.,Yoon, D.H. 한국결정성장학회 2006 한국결정성장학회지 Vol.16 No.6

Micro-pulling down$({\mu}-PD)$법을 이용하여 직경 $0.8{\sim}1.0mm$, 길이 $30{\sim}35mm$의 ZnO가 첨가된 near-stoichiometric $LiNbO_3$, 단결정을 성장하였다. 성장된 결정의 결정구조를 powder x-tay diffraction(XRD) patterns으로 확인하였고, electron probe micro analysis(EPMA)를 이용하여 결정내 Zn 이온들이 균일하게 분포되었음을 확인하였다. 또한 2 mol% 이상의 ZnO 첨가시 $OH^-$ 흡수밴드의 특성이 크게 변화함을 관찰함으로써 ZnO 첨가량에 일치한 역치(threshold)가 존재함을 확인하였다. ZnO-doped near-stoichiometric $LiNbO_3$ single crystals of $0.8{\sim}1.0mm$ diameter and $30{\sim}35mm$ length were grown by the micro-pulling down (U-PD) method. The structure of the grown crystals was confirmed by powder x-ray diffraction (XRD) patterns. Electron probe micro analysis (EPMA) showed that Zn ions were homogeneously incorporated In grown crystals. The threshold in ZnO doping level was confirmed that an abrupt change in the features of $OH^-$ absorption band as doping level reaching about 2 mol%.