유전 및 육종 : 초위성체 표지를 이용한 한국재래돼지 집단의 분자유전학적 고찰

연성흠 ( Seong Heum Yeon ),이승수 ( Seung Soo Lee ),조창연 ( Chang Yeon Cho ),진현주 ( Hyun Ju Jin ),유용희 ( Yong Hee Yoo ),이풍연 ( Poong Yeon Lee ),위미순 ( Mi Soon Wee ),고응규 ( Yeoung Gyu Ko ),손준규 ( Jun Kyu Son ) 한국동물자원과학회(구 한국축산학회) 2011 한국축산학회지 Vol.53 No.1

초위성체(MS) 표지를 이용하여 한국재래돼지 집단의 각각의 분자유전학적 특성을 조사하고 그 평가를 통해 한국재래돼지에 대한 품종 및 계통분류의 기초를 마련하고자 본 연구를 수행하였다. 또한, 각 재래돼지 집단 내 및 집단간의 유전적 변이성을 확인하고, 그 분류 및 특성평가를 위한 MS 분석체계를 마련하여 국내 가축유전자원 관리에 활용하고자 하였다. 국내 관리기관 및 농가에서 보유하고 있는 6개 재래돼지 집단을 중국의 4개 재래돼지 집단 및 외래종 돼지 7개 집단과 함께 분석하였다. 도합 17집단 648두를 대상으로 26개 MS 표지로 분석한 결과, 한국재래돼지 집단은 외래종과 중국재래돼지로부터 분자유전학적으로 별개의 집단으로 확연히 구분되는 것을 확인하였다. 한국재래돼지 집단의 기대이형접합도(HE)는 0.65의 값을 보인 두 집단(B, D)을 제외한 나머지에서 0.48~0.55의 수준을 보여 전반적으로 외래종에 비해 낮았다. 한국재래돼지 집단간의 유전거리 또한 0.12~0.34 정도로 비교대상에 비하여 낮았다. 분석대상 한국재래돼지 6집단 중 세 개의 집단은 높은 유전적 균일도를 보였으나, 두 집단에서는 일부 집단의 혼입을, 나머지 하나의 집단에서는 둘 이상의 집단으로부터의 복잡한 혼입이 의심되는 매우 낮은 유전적 균일도를 확인하였다. 본 연구를 통하여 한국재래돼지 집단간의 유전적 차이 및 동질성, 그리고 집단내의 유전적 균일성을 확인하였다. 이러한 결과는 국내유전자원의 고유성을 인정할 수 있는 과학적인 근거로서, 국가수준의 가축유전자원 평가, 관리의 기초자료로 활용될 수 있을 것이다. The study was conducted to select and optimize microsatellite(MS) markers for evaluation of Korean native pig(KNP) populations in order to provide standard for the classification and breed definition of the indigenous breeds. The study also aimed to characterize and classify each KNP populations. A total of 648 pigs from 17 pig populations including six KNP, four Chinese native pig and four commercial pig populations were analyzed with 26 MS markers. KNP populations formed separate cluster from those of Chinese native pig and introduced pig populations. Expected heterozygosity(He) of KNP populations were 0.48~0.55 except two populations with 0.65. Genetic distances between KNP populations were relatively shorter: 0.12-0.34. Among six KNP populations, three showed high genetic uniformity, two showed lower uniformity and one showed high level of impurity and heterozygosity. The results can be used to evaluate and manage animal genetic resources at national scale.

고상식 육성비육돈사에 적합한 환기시스템에 관한 연구

유용희,송준익,최동윤,정의수,전경호,이풍연,김상우,정종원,Yoo, Yong-Hee,Song, Jun-Ik,Choi, Dong-Yoon,Chung, Eui-Soo,Jeon, Kyoung-Ho,Lee, Poong-Yeon,Kim, Sang-Woo,Jeung, Jong-Won 한국축산환경학회 2010 축산시설환경학회지 Vol.16 No.1

본 연구 국내 여건에 적합한 돈사시설과 분뇨처리가 일체형으로 조합된 고상식 돈사를 개발하는데 있어 1차적으로 적합한 환기 시스템 선발을 하고자 수행하였다. 고상식 돈사는 가로 29m, 세로 9m, 높이 7.5m 이며, l층 높이는 3.1m, 2층 높이는 2.4m 설계 건축하였다. 각 처리별 환기시스템은 l층으로 부터 2층 중천장까지 처리별로 칸막이를 고상식 돈사 내에 설치하였다. 각 처리별 환기방식은 덕트입기$\rightarrow$측벽배기 (처리 V1), 처마입기 (측벽 천장)$\rightarrow$측벽배기 (처리 V2), 천장입기$\rightarrow$측벽배기 (처리 V3) 3개 환기방식을 조사하였다. 공기흐름도와 공기유속을 조사하였다. 공기유속 조사시 환기량은 최소, 중간, 최대로 설정하였다. 그 결과는 다음과 같다. 덕트입기에서 공기흐름도는 2층과 l 층에서 비교적 균일한 수직 하강흐름을 보였고, 처마입기 (측벽천장)는 2층과 1층에서 대칭의 공기유동 하강흐름을 보였다. 천장입기는 중천장을 따라 공기가 우산형태로 퍼지면서 배출되고 느린 공기유속 보이며 l층으로 하강흐름을 보였다 최소환기량일때 하부지점 (돈방 바닥으로부터 80 cm)의 공기유속은 덕트입기, 처마입기 그리고 천장입기 비슷한 경향이었다. 최대환기량일때 덕트입기에서 하부지점의 공기유속이 (0.10~0.26 m/s)로 변화폭이 적었고, 처마입기에서는 공기유속은(0.12~0.63 m/s)로 변화의 폭이 넓고, 천장엽 기는 공기유속이 (0.07~0.15 m/s)로 느리며 변화폭이 적었다. 이상의 결과 덕트입기를 고상식돈사에서 적용이 가능할 것으로 사료되나, 돼지 사양과 실증시험으로 좀 더 검증이 필요할 것으로 판단된다. The goal of this study was to develop a suitable ventilation system for high-rise hog building (HRHB) for growing-fattening with combined slatted floor pen in second story and in situ manure management system in Korea. The HRHB was constructed as 29m long, 9m wide and 7.6m high for outer dimension with an indoor height of 3.1m and 2.4 for lower and upper floor, respectively. Ventilation systems for each treatment were installed in separated rooms of HRHB. The ventilation types installed in each room were following 3 types: ventilation type 1 (V1), where air was pulled through a circular duct inlet and exhausted by fans; ventilation type 2 (V2), where air was pulled through eave inlet (side ceiling inlet) and exhausted by fans; and ventilation type 3 (V3), where air was pulled through baffled ceiling inlet and exhausted by fans. For each ventilation system, investigated air velocity under minimum, medium and maximum ventilation ratio and air flow pattern inside. The results were as follows; For air flow pattern from top to bottom, V1 showed a homogeneous vertical type, V2 showed a bilateral symmetry type and V3 showed an vertical umbrella type. Under minimum ventilation ratio, air velocity in upper floor (80cm above the slated floor) was similar for V1, V2, and V3. Under maximum ventilation ratio, air velocity in upper floor was undeviating for V1 (0.10~0.26m/s) and varied for V2 (0.12~0.63m/s) while those for V3 was relatively slow and less varied (0.07~0.15m/s). In conclusion, Duct inlet type (V1) can be applied to the development of a new HRHB with additional evaluations such as field test hog feeding.

韓國人에서 血友病 B 遺傳子(血液凝固因子 Ⅸ)의 構造 및 制限酵素 Dde Ⅰ, MnI Ⅰ 切片길이 多形性에 關한 分子 遺傳學的 考察

이풍연,전봉균,이정민,권오병,권무식 성균관대학교 생명과학자원연구소 1996 生命資源科學硏究 Vol.2 No.2

Hemophilia B, a human chromosome X-linked recessive disease, is a bleeding disorder resulting from defect or abnormality in blood coagulation factor IX. DNA-based prenatal diagnosis or carrier detection for hemophilia B in Korean has been developed by analyzing restriction fragment length polymorphisms(RFLPs). Two polymorphisms( Dde I and Mnl I) were investigated as follow. Genomic DNAs were extracted from blood of 50 females at the age of twenty. The primers were chemically synthesized by the method of phosphoamidite. Mnl I primers were derived from exon 6, while Dde I primers, from the flanking sequences of intron 1 of the factor IX gene. Genomic DNAs were amplified with Mnl I primers to generate 405 nts long fragments in all cases. They were digested with Mnl I to analyze the polymorphic site on agarose gel. No MnlI polymorphic site was found in all cases. Also, the genomic DNAs were amplified with Dde I primers to generate ca. 320 nts long fragments in all cases. These results are not correlated with those obtained from the Caucasian. It suggests that Korean could exhibit different patterns of Dde I and Mnl I polymorphisms in the gene for blood coagulation factor IX. Direct sequencing of the polymorpic sites will confirm the above sugestion.

한국인에서 혈액응고인자 Ⅷ 유전자의 제한효소 절편길이 다형성에 관한 연구

전봉균,이풍연,권무식 성균관대학교 생명과학자원연구소 1994 生命資源科學硏究 Vol.1 No.2

Hemophilia A, an X chromosome-linked bleeding disorder affecting 1 in 5,000 males worldwide, due to the defect of blood coagulation factor Ⅷ. The wide range of clinical severity exhibited by haemophiliacs plus the hight incidence of sporadic cases suggest that hemophilia A be caused heterogeneous mutation of the gene. Restriction fragment length polymorphism(RFLP) is being used for prenatal diagnosis or carrier detection of the genetic disorder, However, the polymorphisms exhibits heterogeniety among races indicating that data from a people may not be applied to others. Two polymorphic sites(Bcl Ⅰ and Hind Ⅲ) of the factor Ⅷ gene were examined in Korean employing polymerase chain reaction(PCR). The PCR product of Bcl Ⅰ restriction was 948 nucleotides(nts) long, while that of Hind Ⅲ was 730 nts long. The former(Bcl Ⅰ) can generate three fragments(90nts, 100nts, and 480nts from 5' to 3' direction) with the polymorphic site, or two fragments(190nts and 480nts from 5' to 3' direction) wihout the polymorphic site. The latter(Hind Ⅲ) can generate three fragments(148nts, 286nts, and 514nts from 5' to 3' direction) with the polymorphic site, or two fragments(434nts and 514nts from 5' to 3' direction) without the polymorphic site. The heterozygote frequency calculated from the allele frequencies(0.754/0.246) of the Bcl Ⅰ (intron 18) polymorphism was 37.1%. The heterozygote frequency calculated from the allele frequencies(0.807/0.193) of the Hind Ⅲ polymorphism(intron 19) was 31.2%. Thus, the two intragenic polymorphisms predicted to be informative was 56.7% in these studies.

배추(Brassica campestris L. var. pekinensis Makino)의 cDNA library 구축 및 상동성 비교

안주미,권오병,전봉균,이풍연,이정민,권무식 성균관대학교 생명과학자원연구소 1995 生命資源科學硏究 Vol.2 No.1

Expressed sequence tag(EST) has a good value to discover a new gene or to study its structure. Some 20 ESTs were generated to obtain new genetic resources of chinese cabbage(Brassian campestris L. var. pekinensis Makino). Poly A+ RNAs were isolated from 10-day-old seedlings grown at 25℃ under the day light. cDNA gene bank was constructed using λ ZAP /cDNA synthesis /Giga Pack Gold Packing kit. About a million clones were able to obtain from the library. All the clones examined so far had insert DNAs. Nucleotide compositions of randomly selected clones were determined by the Sanger mthod. The DNA sequences were compared with those deposited in the GenePept and GenBank database to figure out nucleotide homologies. Two ESTs showed significant similarities to the enlisted sequences. They are chloroplast GADPH subunit of Arabidopsis thaliana and carbonic anhydrase of A. thaliana. The full DNA sequences of the two clones are being determined. The cDNA gene bank constructed. in this experiment will being used to isolate more genes induced by the light in the plant.

한국인에서 혈액응고인자 IX 유전자의 제한효소 절편길이 다형성에 관한 연구

전봉균,이풍연,권오병,이정민,안주미,권무식 성균관대학교 생명과학자원연구소 1995 生命資源科學硏究 Vol.2 No.1

DNA-based prenatal diagnosis or carrier detection for hemophilia B in Korean has been developed by RFLPs. The polymorphisms were Taq I and Xmn I . Genomic DNAs were extracted from the blood of 56 females and 10 males at the age of twenty. The PCR primers were chemically synthesized by the method of phosphoramidite. Taq I-59 & -39 derived from flanking sequences of intron 4 generate 163nt fragment. And, Xmn 1-59 & -39, derived from the flanking sequences of intron 3 allow amplification 222nt fragment. Using Polymerase chain reaction(PCR) method, each segment(intron 3-Xmn I, intron 4-Taq I) was amplified with each primers and the genomic DNA as template. The amplified DNA fragments were treated with an appropriate restriction enzyme. The Xmn I polymorphism of the factor IX gene was detected on agarose gel as segments of 154 and 68nt, respectively. The heterozygote frequency, calculated from the allele frequencies (0.025/0.975) in intron 3-Xmn I polymorphism, was 4.8%. In case of intron 4-Taq I polymorphism, there is no polymorphic site. So that the heterozygote frequency calculated from the allele frequencies (0.0/1.0) in intron 4-Taq I polymorphism, was 0%. Thus, the two intragenic polymorphisms predicted to be informative was 4.8% in this study. The results are not correlated with those obtained from the Caucasian. It suggests that Korean exhibit different patterns of Xmn I and Taq I polymorphism in the human coagulation factor IX gene.