건조숙성에 따른 저등급 한우 채끝 등심의 품질 증진

이철우,이승호,민예진,이수기,조철훈,정사무엘,Lee, Chul Woo,Lee, Seung Ho,Min, Yejin,Lee, Sookee,Jo, Cheorun,Jung, Samooel 한국식품영양학회 2015 韓國食品營養學會誌 Vol.28 No.3

This study investigated the quality change of strip loin from hanwoo with quality grade 2 produced by dry aging, and compared the quality of dry-aged strip loin with that of strip loin from hanwoo with quality grade $1^+$. Hanwoo strip loins with quality grade $1^+$ (SL1) and 2 (SL2) after a day of slaughter and dry-aged strip loin (DSL2) aged for 21 days at $2^{\circ}C$ with 85% humidity were obtained from local markets. The proximate composition, pH, cooking loss, lipid oxidation, instrumental color (CIE $L^*$, $a^*$ and $b^*$ value) and sensory properties of the strip loins were measured. DSL2 contained low moisture and high protein contents compared with SL2, and low fat, high protein, and high ash contents compared with SL1 (p<0.05). The pH and cooking loss were higher and lower, respectively, in DSL2 compared to SL2 (p<0.05). SL1 had a higher pH than DSL2 (p<0.05). However, the cooking loss was not different between SL1 and DSL2. The TBARS value was the highest in DSL2 and the lowest in SL2 (p<0.05). DSL2 had higher $L^*$ and $b^*$ value compared with SL2 (p<0.05). There were no significant differences of $L^*$, $a^*$ and $b^*$ values between DSL2 and SL1. Sensory properties such as the color, flavor, taste, texture, and acceptability of DSL2 were higher than those of SL2 (p<0.05). There was no significant different in all sensory properties between DSL2 and SL1. According to the results, dry aging can improve the nutritional and sensorial quality of strip loin with quality grade 2 to quality comparable to quality grade $1^+$.

임상증상을 보이는 만성 경막하혈종 환자에 대한 소천공배액술의 치료결과

이철우,황선철,김범태,이세영,임수빈,신원한,Lee, Chul-Woo,Hwang, Sun-Chul,Kim, Bum-Tae,Lee, Se-Young,Im, Soo-Bin,Shin, Won-Han 대한신경외과학회 2005 Journal of Korean neurosurgical society Vol.37 No.4

Objective: Symptomatic chronic subdural hematoma(CSDH) is a well-known neurosurgical entity and most of the lesion is managed by surgical treatment. The authors analyze the surgical indication and the treatment results of twist drill craniostomy with closed-system drainage(TDD) for the symptomatic CSDH. Methods: From March 2001 through December 2003, 31 patients who were treated with TDD for the symptomatic CSDH and followed more than 6months were included. The radiologic criteria of TDD in this study were 1) homogeneous density of hematoma on computed tomography(CT), 2) no septation of hematoma on magnetic resonance imaging(MRI), and 3) thicker hematoma more than twice thickness of skull. Surgical procedures were performed on the maximum thickness of hematoma on CT/MRI. Short and long Steinman pins were used to penetrate the skull and hematoma membrane. As the 5L catheter was inserted through the drill hole, it was kept for 1 - 7days for the drainage of CSDH. The patients of CSDH were followed with clinical symptoms and CT studies. Results: Most of all the 31 patients were improved. However, one patient was suffered from postoperative epidural hematoma and the other patients have received the secondary operation because of the recurrence of CSDH on 3 months after initial surgery. Conclusion: TDD is safe procedure for the symptomatic CSDH if the patients are selected appropriately.

NIH3T3와 NIH3T3(ras) 세포에서 Dynamin II 발현 및 형태적 비교

이철우,김수관,최정윤,최백동,배춘식,정순정,정문진,Lee, Chul-Woo,Kim, Su-Gwan,Choi, Jeong-Yun,Choi, Baik-Dong,Bae, Chun-Sik,Jeong, Soon-Jeong,Jeong, Moon-Jin 한국현미경학회 2005 Applied microscopy Vol.35 No.3

Ras 신호전달체계는 세포내 다양한 결합 분자들과 더불어 세포의 분열과 세포의 이동에 관여한다. Dynamin 단백질은 endocytosis와 분비과정에서 vesicle를 분리하는데 관여하는 것으로 알려져 있으며, 3가지 아형으로 구분된다. Dynamin I은 신경조직에서 만 발현되고, dynamin II는 모든 조직에서 발현되지만 dynamin III는 정소를 포함한 생식기계에서만 발현된다. 선행된 연구에서 NIH3T3 세포를 이용하여 ras과발현 세포주를 만들었으며, dynamin II와 ras의 신호전달체계에 있는 Grb2가 결합한다는 것을 보고하였다. 따라서, 본 연구는 ras 단백질이 과발현되는 세포 (NIH3T3 (ras))와 대조세포인 NIH3T3의 형태학적인 차이점을 분석하고, 이 두 세포들에서 dynamin II 단백질의 발현의 차이를 비교하고자 하였다. Dynamin II의 발현차이를 분석하기 위해 형광염색을 하여 공초점 레이저현미경으로 세포내 분석을 하였으며, western blot을 시행하여 생화학적인 발현차이를 보았다. 또한, 두 세포의 미세구조적인 분석을 위하여 SEM과 TEM을 사용하였다. Dynamin II는 NIH3T3 (ras) 세포에서 발현이 증가 하였으며, NIH3T3 세포에 비하여 좀더 방추형이 었으며, 작은 세포질 돌기가 세포막을 따라 다수 신장되어있음이 관찰되었다. 또한, NIH3T3 (ras) 세포의 endocytotic vesicle이 형성되는 부위에서 dynamin II의 발현이 증가하였다. 이러한 결과로 dynamin II는 ras신호전달체계의한 신호전달분자로서 작용을 할 것으로 사료된다. It has been known that ras signaling transduction leads to cell proliferation and migration including various adaptor molecules. Dynamin protein has been implicated in the formation of nascent vesicles in both the endocytic and secretory pathways. Dynamin was classified into three isoforms: dynamin I is only expressed in neuronal tissue, dynamin II is expressed ubiquitously in all tissue but that of dynamin III is confined to testis. We have reported in previous study that Grb2, binding to ras, was associated with dynamin II in NIH3T3 cells. Therefore we have tried to identify the relative expression of dynamin II according to overexpressed ras protein in ras oncogene transfected cells (NIH3T3 (ras)). For the detection of differential expression of dynamin II, we have used immunofluorescent staining and western blot methods in NIH3T3 and NIH3T3 (ras) cells. Next we have described the morphological differences between NIH3T3 and NIH3T3 (ras) cells using SEM and TEM. From these experiments dynamin II was highly expressed in NIH3T3 (ras) cells. NIH3T3 cells was transformed to more spindle shape with many cell process by transfection of ras oncogene. Moreover dynamin II was more concentrated in endocytotic membrane of the NIH3T3 (ras) cells compared to that of NIH3T3 cells. The present results suggested that dynamin II may involve the intermediate messenger in Ras signaling transduction pathway.

제제설계(製劑設計)에서의 생체내(生體內) 이용솔(利用率)에 관(關)한 연구(硏究)

이철우,Lee, Chul-Woo 한국약제학회 1975 Journal of Pharmaceutical Investigation Vol.5 No.4

The drug release property from the coated tablets which contain 291mg of acetylsalicylic acid were estimated from comparison of the blood level and the urinary excretions after oral ingestion of coated tablets and noncoated tablet in human. The coating materials are 2-methyl-5-vinylpyridine-methylacrylic acid copolymer(MPM), dimethyl aminoethyl methacrylate methyl methacrylate copolymer(EE), polyvinyl acetal diethyl aminoacetate(AEA), and shellac. Each of 7 subjects ingested 873mg of acetylsaliylic acid. All tablets are coated approximately $3.5{\pm}0.5%(w/w)$ per tablet with each of the coating materials and met K.P.II. standard for potency and disintegration time. The availability was decreased in the following order:MPM coated tablets>EE coated tablets>AEA coated tablets>> shellac coated tablets.

담수산 물벼룩인 Moina macrocopa를 이용한 과불화화합물 PFOA와 PFOS의 독성평가

이철우,김현미,최경희,Lee, Chul-Woo,Kim, Hyun-Mi,Choi, Kyung-Hee 환경독성보건학회 2007 환경독성보건학회지 Vol.22 No.3

Perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate (PFOS) are fully flurorinated organic compounds which are highly persistent in environment and accumulated in organism. These chemicals are released to the environment at their manufacture, during their use in industrial and consumer applications and from disposal after their use. The purpose of this study was to determine the effects of PFOA and PFOS on the freshwater flea (Moina macrocopa). Acute toxicity test and chronic toxicity test were performed for 2 days and 10 days, respectively. Acute toxicity was assessed on the basis of mortality, while chronic toxicity was assessed by fecundity as well as mortality. The acute toxicity studies on PFOA and PFOS showed that the values of $LC_{50}$ were $73.9\;mg/L\;and\;27.7\;mg/L$ respectively. In the chronic toxicity test, fecundity was reduced significantly at 24.1 mg/L of PFOA and 9.3 mg/L of PFOS, respectively. Conclusively, the results of this work suggest that Moina macrocopa could be a suitable model organism for screening and assessing of environmental pollutants in water.

악관절 강직증 치료에 있어 측두근 및 근막의 이용

이철우,여환호,김영균,이효빈,Lee, Chul-Woo,Yeo, Hwan-Ho,Kim, Young-Gyun,Lee, Hyo-Bin 대한악안면성형재건외과학회 1993 Maxillofacial Plastic Reconstructive Surgery Vol.15 No.1

Bony ankylosis is an intraarticular condition where there is fusion of the bony surfaces of the joint : The condyle and the glenoid fossa. It occurs in both children and adults, unfortunately more frequently in the former, in whom early recognition and correction is particularly critical. Trauma is well proven to be the predominant cause of TMJ ankylosis. Infection, rheumatoid arthritis and neoplasm are another, significant cases of TMJ ankylosis. The necessity for using an interpositional material to prevent TMJ reankylosis has been widely discussed and many interpositional materials have been used, including temporal muscle and fascia, dermis, auricular cartilage, fascia lata, fat, Lyo-dura, Silastic and various metals. The temporal muscle and fascia have been widely used pedicled flap for head and neck reconstruction. The use of a temporal muscle and fascia for reconstruction of the TMJ, particularly in cases of ankylosis is a very reasonable option. Its principle advantages are its autogenous nature, resilience, and proximity to the joint, allowing for a pedicled transfer of vascularized tissue into the joint area. However, the viability of temporal muscle and fascia is a critical question. We treated 2 cases of TMJ ankylosis with temporal muscle and fascia transfer and one case with temporal fascia. We obtained satisfactory results as to functional aspects.

골내낭에 매식된 수종의 생체요법재료에 대한 조직학적 연구

이철우,최상묵,한수부,박상현,김현종,Lee, Chul-Woo,Choi, Sang-Mook,Han, Soo-Boo,Park, Sang-Hyun,Kim, Hyeon-Jong 대한치주과학회 1996 Journal of Periodontal & Implant Science Vol.26 No.1

The purpose of this study was designed to compare with the effects of 4 different surface active bioceramics on the healing process of alveolar bone defects in dogs. Artificial alveolar bone defects depth 4-6mm, width 3-4mm) were created with # 6 round bur at interproximal areas of maxillary canine, maxillary 2nd premolar, mandibular canine, and mandibular 3rd premolar. porous hydroxyapatite(Interpore $200^R$) , 45S5 bioglass, CJ4/lOC crystalline glass, and JJ crystalline glass were implanted in intrabony defects randomly. Experimental groups were divided into 4 categories according to its implant material. After implantation, all groups were examined postoperatively 4 weeks to 12 weeks. 3 dogs was selected randomly and sacrificed after vascular perfusion with 2.5% glutaraldehyde at every 4 weeks. Tissue blocks with surroundig alveolar bone and soft tissues were removed and immersed in formaldehyde/glutaraldehyde fixative. After 20 weeks decalcification with EDTA and formic acid, sections were made and observed under light microscope and transmission electron microscope. In all experimental groups, the encapsulation of inactive connective tissue was observed around graft particles in 4 weeks. As time elapsed, the thickness of surrounding connective tissue was decreased. Osteoconductive bone growth pattern was seen apparently in all groups. CJ4/lOC crystalline glass showed the most active bone formation until 8 weeks. 45S5 bioglass was, however, the most active in new bone formation at 12 weeks. Though there was difference in resorption rate among grafting materials, the size of graft particles was decreased gradually. 45S5 bioglass was resorbed faster than the others. On the other hand, porous hydroxyapatite was degraded most slowly. Phagocytosed particulate matters was observed in the cytoplasm of multinuclear multinuclear giant cell and macrophage under transmission electron microscope. The results suggested suggested that 45S5 bioglass and CJ4/lOC crystalline glass may have some enhanced reparative potential when compared to porous hydroxapatite in the treatment of periodontal defeds. JJ crystalline glass reguires a further investigation of the safety of its use.

Homogeneous precipitation method를 통한 나노 YAG : Ce 형광체 합성과 광학 특성

이철우,권석빈,지은경,송영현,정병우,김은영,정몽권,윤대호,Lee, Chul Woo,Kwon, Seok Bin,Ji, Eun Kyung,Song, Young Hyun,Jeong, Byung Woo,Kim, Eun Young,Jung, Mong Kwon,Yoon, Dae-Ho 한국결정성장학회 2017 한국결정성장학회지 Vol.27 No.1

본 연구에서는 homogeneous precipitation method를 통하여 구형의 단분산(monodispersed) YAG : $Ce^{3+}$를 합성했다. 단분산 YAG : $Ce^{3+}$의 전구체를 합성하는 과정에서 aluminum ion들이 먼저 석출되어 aluminum 화합물을 형성하고 후에 yttrium 화합물들이 aluminum 화합물들의 표면에서 석출된다. 합성된 전구체를 파우더형태로 얻기 위해 건조과정을 거치는데, oven에서 건조했을 때 보다 동결건조기에서 건조했을 때 비교적 구형의 단분산 YAG : $Ce^{3+}$ 입자를 얻을 수 있었다. 하소 과정에서 공정을 진행하는 온도로서 $1100^{\circ}C$와 $1200^{\circ}C$를 비교해 보았다. 실험 결과 $1200^{\circ}C$의 온도로 상압에서 6시간 동안의 하소 과정을 진행하였을 때 400~500 nm 입자크기를 가진 단분산된 구형의 나노 YAG : $Ce^{3+}$ 입자가 합성되었다. In this study, spherical monodispersed cerium-doped yttrium aluminum garnet (YAG : $Ce^{3+}$) phosphor particles were synthesized via homogeneous precipitation method using the mixed solution of yttrium nitrate, cerium nitrate, aluminum nitrate, ammonium aluminum sulfate, and urea as a precipitant. During the process of precursors of monodispersed YAG : $Ce^{3+}$, aluminum ions which form spherical aluminum compounds precipitated first and yttrium compounds precipitated onto the surface of the existing spherical aluminum compounds. Drying process using lyophilization could obtain monodispered spherical YAG : $Ce^{3+}$ particles compare to using oven. The thermal calcination process of YAG : $Ce^{3+}$ precursors under the temperature of $1200^{\circ}C$ for 6 h was enough to obtain 400~500 nm sized YAG particles with pure YAG phase.

Gel형태의 구연산 제제가 잔류된 치태, 치석 및 치근표면에 미치는 영향

이철우,최상묵,심창구,한수부,Lee, Chul-Woo,Choi, Sang-Mook,Shim, Chang-Koo,Han, Soo-Boo 대한치주과학회 1990 Journal of Periodontal & Implant Science Vol.20 No.2

脊髓脂肪腫

李哲雨(Chul Woo Lee),孫鍵(Kun Sohn),金相喆(Sang Chul Kim) 대한외과학회 1968 Annals of Surgical Treatment and Research(ASRT) Vol.10 No.1