http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
간염바이러스 X단백질 유전자를 핵산전달감염시킨 간암세포주에서 17-DMAG의 항종양효과 상승 효과
이철승 ( Chul-seung Lee ),김옥희 ( Ok-hee Kim ),홍하은 ( Ha-eun Hong ),전상진 ( Sang-jin Jeon ),원성수 ( Seong-soo Won ),김세준 ( Say-june Kim ) 대한간암학회 2017 대한간암학회지 Vol.17 No.2
Background/Aims: Hepatitis B viral protein X (HBx) is implicated in the pathogenesis of hepatocellular carcinoma (HCC) as well as the elevation of heat shock proteins (HSPs) after hepatitis B virus (HBV) infection. We thus investigated the anticancer effects of an HSP90 inhibitor 17-Dimethylaminoethylamino-17-demethoxygeldanamycin (17-DMAG) in HBx- transfected hepatocellular carcinoma cells. Methods: pcDNA-HBx was made by inserting the HBx gene derived from the HBV-infected patient into pcDNA3.1 using the restriction enzymes (XbaI/HindIII). HBx-expressing HepG2 cells were then generated by transfecting HepG2 cells with pcDNA containing HBx gene. To compare the anticancer effects of 17-DMAG between pcDNA-HBx transfected HepG2 cells and the control cells (pcDNA-transfected HepG2 cells), we performed various molecular studies, including Ez-cytox proliferation assay, Western blot analysis, and flow cytometry. Results: 17-DMAG inhibited the proliferation of pcDNA-HBx transfected HepG2 cells better than control cells (P<0.05). After treating with a various concentration of 17-DMAG (50-1,000 nM), pcDNA-HBx transfected HepG2 cells exhibited higher expression of pro-apoptotic proteins (c-caspase-3, c-caspase-8, and c-caspase-9) than did control cells (P<0.05). pcDNA-HBx transfected HepG2 cells showed higher activities of caspase-3, caspase-8, and caspase-9 than did control cells (P<0.05). Finally, we found that the expression of pro-apoptotic proteins (PARP and c-caspase-3) was considerably decreased by the use of a caspase inhibitor suggesting that 17-DMAG induces the cell death of HepG2 cells caspase-dependently. Conclusions: Our study strongly suggests that 17-DMAG has antiviral effects against HBV as well as anticancer effects against HepG2 cells. Thus, the application of 17-DMAG appears to be particularly advantageous to the HCC patients related with HBV infection. (J Liver Cancer 2017;17:126-135)
A₂O 공정을 이용한 저 C/N 비 하수의 질소·인 제거에 관한 연구
이철승,안철우,박진식,김수생 동아대학교 해양자원연구소 2001 硏究報告 Vol.24 No.1
In this study, a pilot-scale A₂O process was applied to remove organics, suspended solids, nitrogen from municipal wastewater with low C/N ratio, and to investigate the treatment efficiency of pilot plant as well as the nitrogen removal characteristics of pilot plant. As a result of studying BOD_(5) and COD removal efficiencies were increase for HRT increase and effluent concentration were to decrease. T-N removal efficiency and concentration of effluent were shown as 60 ~ 80% and below 15mg/L. In aerobic basin, removal efficiency of NH₄-N was shown over 97% with NH₄-N volume load 0.l6kgNH₄-N /㎡·d and in anoxic basin, denitrification efficiency was indicated over 80% with return sludge rate 0.5Q and internal recirculation rate 2.5Q. Removal efficiency and effluent concentration of phosphorus mere shown over 80% and below 2mg/L with return sludge rate 0.5Q.
유총일,이철승,김익곤 대한화학요법학회 1985 대한화학요법학회지 Vol.3 No.2
Osteosarcoma is, except for multiple myeloma, the most common of primary malignant bone tumor, and has previously proved resistant to most chemotherapeutic agents. Intensive effects have been made in the past, however, to improve prognoses, and the development of current adjuvant chemotherapeutic regimens correlates with improved overall survival times in patients with osteosarcoma. The authors, in two cases of osteosarcoma, 1) perfused the affected limb with adriamycin, 2) performed amputation, and 3) gave adjuvant chemotherapy, and have since been following up their prognoses.