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이준철,김도연,오덕재,장호남 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.5
A rCHO cell line of DUKX origin 26*-320, producing recombinant antibody against the human platelet, was cultivated in a two-stage depth filter perfusion system (DFPS) for 20 days in order to attain high recombinant antibody concentration. The productivity of the first stage DFPS bioreactor reached 53 times that of the batch culture in a controlled stirred tank reactor and was showed 12.1 mg/L antibody concentration at a perfusion rate of 6.0 d-¹. Glucose concentration in the first DFPS was maintained at 1.5 g/L to avoid cell damage in the perfusion culture. A second stage DFPS system was attached to the first DFPS, which resulted in a low glucose concentration of 0.02 g/L and a high antibody concentration of 23.9 mg/L. The two-stage depth filter perfusion culture yielded 60% higher product concentration than the batch and 49-fold higher produc-tivity of 69.3 mg/L/d in comparison with that (1.4 mg/L/d) in a batch system. Furthermore, antibody concentration of the sec-ond stage was 97% higher than that of the first stage, and the antibody productivities were comparable to that of the first stage. This two-stage DFPS system also showed potential for higher titer production of recombinant antibody and high volumetric productivity for long-term culture of bio-pharmaceutical substances.
이준철,김신범 한국환경보건학회 2019 한국환경보건학회지 Vol.45 No.4
The recent humidifier disinfectant disaster has caused a great change in our society and lead the Korean people to lose faith in the government and corporations. To overcome this mistrust, the government and corporations have undertaken certain efforts, including a voluntary agreement on consumer product safety. All the ingredients of a consumer product should be disclosed according to this voluntary agreement. There were also unexpected benefits occuring from this process. We found that the concept of corporate responsibility for product safety differed greatly among the various actors in Korean society. Corporations believed that a product could be considered safe if it contained no regulated substances. We have come to believe that this may be an important cause of the humidifier disinfectant disaster. The Korean government, corporations, and civil organizations have reached a consensus that it is a corporation's responsibility to evaluate the risks of products before they are introduced to the market. However, the limitations of these voluntary efforts are clear, and the legal system needs to be improved. We suggest that the concept of extreme recklessness should be introduced into regulations on product safety.
이준철,신성우,김화중,이창준 국제구조공학회 2016 Smart Structures and Systems, An International Jou Vol.17 No.1
The evolution of the electro-mechanical impedance (EMI) of a piezoelectricity (PZT) sensor was investigated to determine the setting times of cement paste in this study. The PZT sensor coated with non-conductive acrylic resin was embedded in fresh cement paste and the EMI signatures were continuously monitored. Vicat needle test and semi-adiabatic calorimetry test were also conducted to validate the EMI sensing technique. Significant changes in the EMI resonance peak magnitude and frequency during the setting period were observed and the setting times determined by EMI sensing technique were relevant to those measured by Vicat needle test and semi-adiabatic calorimetry test.
이준철,김경 국제물리치료연구학회 2016 Journal of International Academy of Physical Ther Vol.7 No.1
In the present study, the general characteristics, job stress, working conditions, and aspects of pain of some industrial workers working in Changwon-si, Gyeongsangnam-do, were surveyed. In analyzing the relationship between job stress and the existence of pain, the variables “working speed” and“ opportunities to develop abilities” were shown to have statistically significant relationships with the existence of pain (p≤.05). Regarding the relationship between working conditions and the existence of pain, the variables“ amount of work per hour,“” amount of work per day,“” number of parts handled during work,“” work production per person,” and“ inconvenient postures or motions during work” were shown to have statistically significant relationships with the existence of pain(P≤.05). Regarding aspects of pain“, within 1~3 years” was the most common answer to time of occurrence of symptoms, with a percentage of 27.6%“; appear almost always” was the most common answer to frequency of symptoms, with a percentage of 37.1%;“ slight pain” was the most common answer to degree of pain, with a percentage of 50.5%; and“ moderate” was the most common answer to encumbrance caused by pain to living and work, with a percentage of 41.2%. The aim of the present study was to determine the factors that affect pain due to musculoskeletal diseases in industrial workers and to define the aspects of pain in order to provide basic data for the preparation of measures to prevent musculoskeletal diseases. To control pain due to musculoskeletal diseases, factors that affect pain, as well as the aspects of pain, should be recognized early, and efforts should be made to supplement and improve systems for preventing recurrence.
이준철 국제물리치료연구학회 2017 Journal of International Academy of Physical Ther Vol.8 No.1
The purpose of this study was to investigate the effects of stable and unstable bridging exercises on the EMG activity of abdominal muscles. Twenty healthy women participated in this study and the muscle activities of left-right rectus abdominis (RA), external oblique (EO), and internal oblique (IO) muscles were recorded during 4 bridging exercises (unilateral/ bilateral, stable/unstable). The activity of the right EO muscle was the highest during unilateral exercise in unstable condition, and left EO muscle also produced the same result. The activity of the right IO muscle was the highest during unilateral exercise in an unstable condition, and left IO muscle also produced the same result. The activity of the right RA muscle was the highest during unilateral exercise in a stable condition, and left RA muscle produced the same result. Unstable/unilateral (USUL) represented the highest activity among the 4 exercises. EO/IO muscles represented the highest activity during the USUL exercise, and RA did so during the stable/unilateral exercise. These results suggest that specific posture (USUL) can be administered targeting a specific side of abdominal muscles.
The Influence of Foot Position on Standing Balance on an Unstable Base of Support
이준철 한국인터넷방송통신학회 2018 International Journal of Internet, Broadcasting an Vol.10 No.2
The purpose of this study was to compare the balance ability at different foot positions using K A T 2000 (Berg, Inc, vista, CA.1994). Thirty (male 15, female 15) normal subjects participated in this study. All subjects were tested at a one leg position or a two leg position that were toe-in 25˚, toe-out 25˚, and 45˚. The starting position was where the subject crossed their arms across the chest and flexed knees slightily. The results of each test were displayed on a screen in a score format, which indicated balance index. These collected data were analyzed by using one way ANOVA, and t-test. The results of this study were as follows: When changing the angle of the foot in the one-foot and two-foot standing positions, there was no statistically significant difference, but the balance performance with the foot rotated by 25˚ was better than that with the foot rotated by 45˚. When changing the direction of the foot in the one-foot and two-foot standing positions, there was no statistically significant difference, but the balance performance with the foot rotated laterally was better - except for the case when the foot was medially rotated by 25˚ in the right-foot static standing position. When the feet were medially rotated by 25˚ in the two-foot static standing position, and were medially rotated by 25˚ and 45˚ in the one-foot static standing position, the balance performance of females was better than that of males. In this study, it was found that the balance performance of the subjects changed when the position of the foot was shifted on an unstable base of support. However, there was little correlation between balance performance and the height, weight and foot length of the subjects. It is necessary to conduct a follow-up study targeting various age groups and those with various diseases using an unstable platform or applying different physical or visual conditions, such as the length of the legs. Physical therapists need to consider the position of the foot in clinical settings for a better balance training or assessment.
전기 임피던스 분광법을 이용한 시멘트계 재료의 응결 특성 평가
이준철,박인용 한국건설순환자원학회 2017 한국건설순환자원학회 논문집 Vol.5 No.4
본 연구에서는 전기 임피던스 분광법을 이용하여 시멘트계 재료의 응결 시점을 평가하였다. 시멘트 페이스트 시편을 제작한후 전기노드를 매립하여 전기 임피던스 응답 스펙트럼의 변화를 연속적으로 모니터링하였으며, 이와 동시에 간이단열시험을통한 수화온도측정과 비카트침 시험을 함께 수행하였다. 전기 임피던스 분광법에서는 시멘트 페이스트의 수화와 동시에 컨덕턴스가 서서히 증가하는 경향을 나타냈다. 이후, 특정시점에 이르러 컨덕턴스는 급격히 감소하는 경향을 나타냈으며, 이후 또다른특정시점에서 완만하게 감소하는 경향을 나타냈다. 이러한 특정 시점은 간이단열시험 및 비카트침 시험에 의해 측정된 응결시점과 부합하는 것으로 나타났다. 이를 통해 전기 임피던스 분광법을 이용하여 시멘트계 재료의 응결을 효과적으로 모니터링하는것이 가능하리라 판단된다.