Ginsenoside Rg1 및 Rb1을 처리한 신경세포주(SH-SY5Y세포)의 유전자 발현양상

이준노,양병환,최승학,김석현,채영규,정경화,이준석,최강주,김영숙,Lee, Joon-Noh,Yang, Byung-Hwan,Choi, Seung-Hak,Kim, Seok-Hyun,Chai, Young-Gyu,Jung, Kyoung-Hwa,Lee, Jun-Seok,Choi, Kang-Ju,Kim, Young-Suk 대한생물정신의학회 2005 생물정신의학 Vol.12 No.1

Objectives:The ginsenoside Rg1 and Rb1, the major components of ginseng saponin, have neurotrophic and neuroprotective effects including promotion of neuronal survival and proliferation, facilitation of learning and memory, and protection from ischemic injury and apoptosis. In this study, to investigate the molecular basis of the effects of ginsenoside on neuron, we analyzed gene expression profiling of SH-SY5Y human neuroblastoma cells treated with ginsenoside Rg1 or Rb1. Methods:SH-SY5Y cells were cultured and treated in triplicate with ginsenoside Rg1 or Rb1($80{\mu}M$, $40{\mu}M$, $20{\mu}M$). The proliferation rates of SH-SY5Y cells were determined by MTT assay and microscopic examination. We used a high density cDNA microarray chip that contained 8K human genes to analyze the gene expression profiles in SH-SY5Y cells. We analyzed using the Significance Analysis of Microarray(SAM) method for identifying genes on a microarray with statistically significant changes in expression. Results:Treatment of SH-SY5Y cells with $80{\mu}M$ ginsenoside Rg1 or Rb1 for 36h showed maximal proliferation compared with other concentrations or control. The results of the microarray experiment yielded 96 genes were upregulated(${\geq}$3 fold) in Rg1 treated cells and 40 genes were up-regulated(${\geq}$2 fold) in Rb1 treated cells. Treatment with ginsenoside Rg1 for 36h induced the expression of some genes associated with protein biosynthesis, regulation of transcription or translation, cell proliferation and growth, neurogenesis and differentiation, regulation of cell cycle, energy transport and others. Genes associated with neurogenesis and neuronal differentiation such as SCG10 and MLP increased in ginsenoside Rg1 treated cells, but such changes did not occur in Rb1-group. Conclusion:Our data provide novel insights into the gene mechanisms involved in possible role for ginsenoside Rg1 or Rb1 in mediating neuronal proliferation or cell viability, which can elicit distinct patterns of gene expression in neuronal cell line. Ginsenoside Rg1 have more broad and strong effects than ginsenoside Rb1 in gene expression and related cellular physiology. In addition, we suggest that SCG10 gene, which is known to be expressed in neuronal differentiation during development and neuronal regeneration during adulthood, may have a role in enhancement of activity dependent synaptic plasticity or cytoskeletal regulation following treatment of ginsenoside Rg1. Further, ginsenoside Rg1 may have a possible role in regeneration of injured neuron, promotion of memory, and prevention from aging or neuronal degeneration.

정신분열병 환자에서 carbamazepine이 혈청 haloperidol 농도에 미치는 영향

이준노,양병환,김미경,신현아,고현철 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

The author examined the sequential changes of serum haloperidol(HP) levels after administration and subsequent withdrawal of carbamazepine(CBZ) in chronic schizophrenic patients for further understanding of the enzyme induction of cytochrome P450(CYP) in relation to these two drugs. The serum HP levels were measured by high performance liquid chromatography(HPLC) with UV detector. Twenty-five patients who were maintained with haloperidol for a month or more completed in this study of CBZ induced sequential changes of the serum HP levels. Subjects were performed with multiple samples at baseline, each 1 week and 4 weeks after CBZ administration and 1 week and 4 weeds after subsequent withdrawal of CBZ. CBZ caused a significant decrease of the serum HP levels at 1 week(t=7.747 p=0.000) and 4 weeks(t=5.899 p=0.000) compared with the baseline levels, but no significant changes of the HP levels at 4 weeks compared with 1 week(t=-0.639 t=0.529). After subsequent withdrawal of CBZ, the serum HP levels are increased at 1 week(t=-3.392 p=0.002) and 4 weeks(t=-3.839 p=0.001) more than those of the HP and CBZ combination therapy for 4 weeks. The changes of HP levels were not significant at 4 weeks compared with 1 week after the withdrawal of CBZ(t=-1.509 p=0.144). There is no difference of the HP levels between baseline and 4 weeks after the withdrawal of CBZ. These results suggest that CBZ decreased serum HP levels by induction of the CYP isozymes which metabolize HP, and CYP induction complete almost within the first week and thereafter it seems to be disappeared. After abrupt discontinuation of CBZ, the HP levels were elevated quite rapidly within a week by deinduction of CYP isozymes, and then the HP level was increased nonsignificantly, ultimately reached the baseline level. The reason that the duration of deinduction was long period more than those of induction seems to be due to the remained CBZ in the body that did not excreted.

비전형적 항정신병약물에 의한 체중증가의 기전 및 약리유전학

이준노,양병환,Lee, Joon-Noh,Yang, Byung-Hwan 대한생물정신의학회 2003 생물정신의학 Vol.10 No.1

The use of atypical antipsychotics is limited by occurrence of adverse reactions such as weight gain, despite of their benefits. This article provides a comprehensive review and discussion of the most significant findings regarding obesity-related pathways and integrates these with the known mechanism of atypical antipsychotic action. The focus of this article is primarily on the genetics of obesity related pathways that may be disrupted by atypical antipsychotics. This review also discussed weight gain, hyperglycemia or occurrence of diabetes while being treated with atypical antipsychotics from the point of view of pharmacogenetics. Pharmacogenetic research seeks to uncover genetic factors that will help clinicians identify the best treatment strategies for their patients. It will aid clinically in the prediction of response and side effects, such as antipsychotic-induced weight gain, and minimize the current "trial and error" approach to prescribing in the near future. This article also presents the genetics of both central and peripheral pathways putatively involved in antipsychotic-induced weight gain while providing a comprehensive review of the obesity literature. This article also review obesity related candidate molecules which may be disrupted during atypical antipsychotic drug treatment.

정신질환자들에 대한 뇌파학적 고찰

이준노,오동렬,양도선,김규현 국립서울정신병원 2001 臨床硏究論文集 Vol.2001 No.-

노인 만성 조현병 환자군에서 병태생리, 인지기능, 병식, 삶의 질 간의 관련성

홍경기,이준노,임선진,김정민,나의현,홍문화,한혜리 대한노인정신의학회 2014 노인정신의학 Vol.18 No.2

Objective:The aim of this study was to investigate the association with psychopathology, cognitive function, insight and quality of life (QOL) in elderly patients with chronic schizophrenia over age 55. Methods:103 schizophrenic patients over age 55 with illness duration over 10 years, are enrolled in a cross-sectional study. The subjects were assessed by the Korean version of 4th Revision of Schizophrenia Quality of Life Scale, Korean Version of Scales to Assessment Unawareness of Mental Disorder, Positive and Negative Syndrome Scale (PANSS) and the cognitive function battery designed for this study. Multiple regression stepwise selection models were executed to identify the relations among variables, and the contributing factors to QOL. Results:Among schizophrenic patients with lower illness-severity with PANSS total score below 75, higher PANSS positive subscale score and lower number of hospitalization were related to lower QOL. Among patients with higher illness-severity with PANSS total score of 75 and over, higher PANSS general psychopathology subscale score, better intelligence, better delayed recall function, worse attention, better awareness of medication effect and later onset were related to lower QOL. Conclusion:Results of our study suggest that improvement in positive symptom and general psychopathology could increase the QOL in elderly patients with chronic schizophrenia over age 55. And the management which could improve attention, awareness of need for medication would attribute the QOL.

알츠하이머 치매 환자에서 성별에 따라 교육 성취도가 영역별 인지기능에 미치는 영향 : A Clinical Research Center for Dementia of South Korea Study

이준노(Jun Ro Lee),이춘수(Chunsoo Lee),최준배(Junbae Choi),강효신(Hyo Shin Kang),임신원(Shin Won Lim),나덕렬(Duk L. Na),김성윤(Seong Yoon Kim),이재홍(Jae-Hong Lee),한설희(Seol-Heui Han),최성혜(Seong Hye Choi),김상윤(Sang Yun Kim),김도관 대한노인정신의학회 2017 노인정신의학 Vol.21 No.2

Objective:We aimed to find the sex differences of the way how educational attainment influence on cognitive function in patients with Alzheimer’s disease (AD). Methods:We recruited 424 patients with AD from a large hospital-based multi-center cohort. We evaluated cognitive subdomains using the Seoul Neuropsychological Screening Battery-Dementia version. We employed multiple linear regression analysis to compare the score of cognitive subdomains between the groups with high versus low educational attainment based on the high school graduate. Interaction between educational attainment and sex was also included in the analysis. Results:High and low educational attainment group had 33.0% and 67.0% of patients in the study population. In the multiple regression analysis, score of all cognitive subdomains were significantly higher in the high educational attainment group. The score of attention was also significantly affected by interaction between educational attainment and sex. This result may be helpful to develop cognitive intervention therapy. Conclusion:In the female patients with AD, educational attainment showed higher influence on the attention score.

장기간 혈액투석을 받는 말기 신부전환자의 정서상태 및 투석 순응도에 대한 연구

이준노,김창현,권택술 大韓神經精神醫學會 1995 신경정신의학 Vol.34 No.4

The purpose of this study is to evaluate the relationship between compliance of patients under hemodialysis and their affective states of depressed mood and anxiety. Subjects consist of 58 end-stage renal failure patients on the long-term hemodialysis of one year or longer. Depressed state was evaluated with Beck Depression Inventery(BDI) and anxiety state was assessed with Self-rating Antiety Scale(SAS). The compliance in terms of diet, medication and fluid was assessed with Potassium(K), Phosphorus(P) and Interdialytic Weight Gain(IWG), respectively. the primary findings are as noted below. 1) 58.6% of the subjects felt depressed, but anxiety level was quite within normal range. 2) There were no intercorrelations between the compliance parameters of diet, education and fluid intake. preliminary correlations are as follows. The level of depression correlated with that of anxiety. Educational level is correlated negatively with scores of depression and anxiety. Duration of marriage is correlated positively with scores of depression. 3) Only anxiety, not depression, correlated with IWG, when controlled for age, sex and other clinical variables. But, anxiety did not correlate with K and P. This result may suggest that IWG is a more sensitive indicator of compliance than K and P.

중학교 과학교과서에서 기공에 관한 학생들의 오개념 연구

정찬노 ( Chan No Chung ),이준상 ( Joon Sang Lee ) 충북대학교 과학교육연구소 2013 과학교육연구논총 Vol.28 No.1·2

Ecosystem could be maintained by the plants that produce sucrose and then consumers in food chain could use the products of photosynthesis. Sucrose is synthesized by using of CO2 and H2O. The CO2 and H2O must be taken in or out through stomata. Therefore, stomata are very important as key conceptions of plant physiology. This research, based on the investigation of the various types of middle school students`` misconceptions about stomata, was performed for the finding of the effective teaching and learning strategies. To test the misconceptions, 10 questions was made in the middle school text books. Total of 200 students had been compared according to their sex and the region where they live in Seoul or rural areas. It was found that most students were having the same types of misconceptions. It was shown that students had misconceptions how stomata open or close and what kinds of materials exchange through stomata. Significant differences in the misconceptions were found according to the questions between male and female students. However, students from rural areas turned out to have more misconceptions than those from Seoul. The conceptions about stomata were not properly taught to the students in the middle school. Therefore, new teaching and learning strategies for students should be developed for the students to construct scientific conceptions correctly.

식도운동질환과 위식도역류질환과의 연관성

홍성노 ( Hong Seong No ),이풍렬 ( Lee Pung Lyeol ),이준행 ( Lee Jun Haeng ),김영호 ( Kim Yeong Ho ),김재준 ( Kim Jae Jun ),백승운 ( Baeg Seung Un ),유병철 ( Yu Byeong Cheol ),이종철 ( Lee Jong Cheol ) 대한소화기학회 2003 대한소화기학회 추계학술대회 Vol.2003 No.-

<목적> 식도 운동의 장애는 위식도역류의 발생과 연관이 있으며, 식도운동질환과 위식도역류질환과의 연관성을 시사하는 여러 연구가 보고되고 있다. 이 중 새로이 분류된 비효과적식도운동(ineffective esophageal motility)은 위식도역류질환의 주요 식도내압검사 소견으로, 일부 연구에서는 위식도역류질환의 표지(marker)라고 주장되기도 하지만 논란이 되고 있으며, 이를 제외한 다른 식도운동질환에서 위식도역류질환의 빈도와 연관성을 살펴본 연

LNG FPSO 액화공정 사이클의 최적 설계

이준채(Joon-Chae Lee),구남국(Namkuk Ku),황지현(Jihyun Hwang),노명일(Myung-Il Roh),이규열(Kyu-Yeul Lee) (사)한국CDE학회 2012 한국 CAD/CAM 학회 학술발표회 논문집 Vol.2012 No.2

LNG FPSO(Liquefied Natural Gas-Floating, Production, Storage and Offloading )에서 액화 공정 시스템(Liquefaction Process System)은 천연가스를 부피가 1/600 인 액체 상태로 액화시키며, LNG FPSO 의 Topside Process System 건조 비용의 70%를 차지하고 있는 핵심 공정이다. 이러한 액화 공정 시스템의 Cycle 을 구성하는 주요장비에는 Compressor, Condenser, Expansion valve, Evaporator, Phase Separator, Common Header 그리고 Tee 가 있으며, 이들을 조합함에 따라 다양한 종류의 액화 Cycle 이 구성된다. 본 논문에서는 이러한 다양한 종류의 액화 Cycle 을 표현할 수 있는 Generic Model 을 제안하였고, LNG FPSO 의 Offshore Application 을 위하여 Generic Model 로부터 도출된 하나의 액화 Cycle 에 대하여 Compressor 에 공급되는 소요 동력을 최소로 하는 최적화된 액화 사이클을 설계하였다. 이를 LNG FPSO 에 적용 검토 중인 Dual Mixed Refrigerant(DMR) Cycle 과 비교 하였으며, 그 결과 본 논문에서 제안한 액화 Cycle 의 Compressor 소요 동력이 약 1.2% 감소한 것을 확인할 수 있었다.