오매(烏梅)의 다성분동시분석 및 마우스를 이용한 경구 단회투여 급성독성시험

이인선,한창현,이철,황방연,정상혁,이영준,전원경,Lee, In-Sun,Han, Chang-Hyun,Lee, Chul,Hwang, Bang-Yeon,Jung, Sang-Hyeok,Lee, Young-Joon,Jeon, Won-Kyung 대한예방한의학회 2011 대한예방한의학회지 Vol.15 No.1

Objectives : Previous studies have shown that Fructus mume (FM) has anti-platelet effects. The present study was performed to determine the acute oral toxicity and quality control of a crude extract of FM in ICR mice. Methods : We investigated the in vivo single dose acute toxicity of FM 95% ethanol extract. This test was orally administered once by gavage to 20 male and 20 female mice at dose levels of 0 (control group), 1250, 2500 and 5000mg/kg body weight, respectively. Mortalities, clinical findings, autopsy findings and body weight changes were monitored daily for the 14 days following the administration. HPLC analysis was performed for the simultaneous determination of ursolic acid and p-hydroxycinnamic acid in FM. Reverse-phase chromatography using a C18 column and photodiode array detection at 211 nm was used for quantification of the two maker components. The mobile phase for gradient elution consists of water and acetonitrile. Results : We observed survival rates, general toxicity, change of body weight, and autopsy. The mice did not die after single oral administration of maximum dose of FM. Autopsy of animal revealed no abnormal gross finding. Therefore, $LD_{50}$ value of FM for ICR mice was more than 5000mg/kg on oral route. The HPLC analysis showed that ursolic acid and p-hydroxycinnamic acid amounts to 9.75- and 0.12% in the extract with the retention times of 47.99- and 15.38 minutes, respectively. Conclusions : These results suggest that no toxic dose level of FM in mice is considered to be more than 5000mg/kg. Consequently, it was concluded that FM have no effect on acute toxicity and side effect in ICR mice. For the quality control of FM extract, simultaneous determination of ursolic acid and p-hydroxycinnamic acid was established.

잘못된 Stent 삽입 치료 후 슬와 동맥 포착 증후군에서 발생한 슬와 동맥의 완전 폐쇄 1예

이인선 ( In Sun Lee ),최동훈 ( Dong Hoon Choi ),김은혜 ( Eun Hye Kim ),신상훈 ( Sang Hun Shin ),이도연 ( Do Yun Lee ),김승환 ( Seong Hwan Kim ),이택연 ( Taek Yeon Lee ) 대한내과학회 2012 대한내과학회지 Vol.83 No.6

Entrapment of the popliteal artery is a rare cause of ischemia of the lower extremities in young males. The development of local occlusive or aneurysmal changes of the popliteal artery is caused by abnormal anatomical relationships between vascular and musculotendinous structures in the popliteal fossa. A 55-year-old male patient visited Yonsei University College of Medicine with the chief complaint of claudication in his right calf. He had undergone stent insertion in the right popliteal artery for peripheral artery occlusive disease 2 years earlier. Lower extremity CT angiography showed stent fracture and in-stent thrombosis. He underwent femoral-to-popliteal artery bypass surgery. Postoperative lower extremity CT angiography showed that the vein graft was compressed between the medial head of the gastrocnemius and the semimembranosus muscle. However, the blood flow was normal and his pain was relieved. (Korean J Med 2012;83:786-790)

도시탐색구조의 활성화 방안에 관한 연구

이인선,신호준,백민호,Lee,In-Sun,Shin,Ho-Joon,Baek,Min-Ho 한국방재학회 2012 한국방재학회논문집 Vol.12 No.5

인구증가 및 경제성장으로 인해 도시의 외적 규모가 급속히 확대되고 고층건물, 지하공간의 고도이용 등 도시공간이 복잡해져 있는 상황에서 도시지역에 자연재난 및 인적재난이 발생 할 경우 막대한 피해가 예상된다. 이에 피해지역의 도시탐색구조 역량강화는 중요한 과제라 할 수 있다. 우리나라는 도시탐색구조 분야의 개념 정립 및 인식이 미비한 단계이며, 전문적인 교육 및 훈련시설의 부족으로 체계적인 대응이 이루어지지 못하고 있는 상황이다. 이에 본 논문에서는 도시탐색구조의 개념을 정리하고 도시탐색구조를 위한 국내 외 조직현황을 분석하였으며 도시탐색구조업무를 담당하는 중앙 119구조대원에 의한 설문조사를 통하여 도시탐색구조의 문제점 및 개선방안을 제시하였다. Population and economic growth have caused the rapid external expansion of cities with high-rises in the metropolitan area, high-intensity utilization of underground space etc. As cities becoming more complex than ever, an outbreak of natural or manmade disaster would result in damages beyond imagination hence the enhancement of Urban Search and Rescue ability in the damaged area becomes crucial. Korea is in its very early stage as far as the concept and awareness of Urban Search and Rescue is concerned, and the lack of professional education and training facilities has prevented the associated bodies to take strategic responses. This paper defined the notion of Urban Search and Rescue, analyzed the status of national and international organizations, conducted surveys on National 119 Rescue Services team members responsible for the rescue operations to figure out problems of Urban Search and Rescue and proposed possible improvement measures.

한방진단시스템 DSOM의 진단결과에 따른 월경통 환자의 허실(虛實) 진단에 대한 연구

이인선,조혜숙,윤현민,정경근,김규곤,박지은,최선미,Lee, In-Sun,Cho, Hye-Sook,Youn, hyoun-Min,Jung, Kyoung-Keun,Kim, Kyu-Kon,Park, Ji-Eun,Choi, Sum-Mi 대한약침학회 2007 Journal of pharmacopuncture Vol.10 No.1

Purpose : This study was undertaken to make a diagnosis weakness and firmness (虛實) of Dysmenorrhea patients by diagnosis questionnaires system(Diagnosis System of Oriental Medicine-DSOM) Methods : The subjects were 58 volunteers who was suffering for dysmenorrhea, employed using Measure of Menstrual Pain (MMP) questionnaire. The had agreed to take part in this experiment, with didn't take any anodyne drugs. The MMP score by using 7 questions and the Menstrual Symptom Severity List(MSSL-D) was measured before and after menstruation cycle. Results and Conclusions : The findings of this study were as follows; 1. We examined Pathogenic Factor's frequency of DSOM, Coldness(寒) was 45 persons 80.36%, Damp(濕) was 40 persons 71.43%, Heart(心) was 37 persons 66.07%, Heat syndrom(熱) was 9 persons 16.07%, insufficiency of Yang(陽虛) was 6 persons 10.71%. 2. We divided Dysmenorrhea patients into two groups(weakness and firmness) by Results of DSOM, Firmness was 25 Persons 43.1%, Weakness was 23 persons 39.7%, Unknown was 10 persons 17.2%. 3. In estimation based on Measure of Menstrual Pain (MMP) questionnaire Severe menstrual pain is weakness, Mild menstrual pain is Firmness. 4. In estimation of coldness and heat syndrom, Coldness was 40 persons 69.0%, Heat syndrom, was 2 persons 3.5%, Possess both coldness and heat syndrom was 9 persons 15.5%.

Trimethylamine Gas 측정을 위한 TiO₂, Pd, Pt 및 In이 첨가된 SnO₂ 가스 센서의 특성

정순분(Soon-Boon Jung),전재목(Jae-Mok Jun),이인선(In-Sun Lee),이형락(Hyeong-Rag Lee),박영호(Young-Ho Park),최성우(Sung-Woo Choi),이창섭(Chang-Seop Lee) 한국가스학회 2007 한국가스학회지 Vol.11 No.1

Ebstein 기형 환자에서 발생한 혈관 베체트병

김정혁 ( Jung Hyuk Kim ),허환 ( Hwan Herr ),방기태 ( Ki Tae Bang ),배강우 ( Kwang Woo Bae ),장현규 ( Hyun Kyu Chang ),이명용 ( Myoung Yong Lee ),이인선 ( In Sun Lee ),윤보영 ( Bo Young Yoon ),이윤우 ( Yun Woo Lee ) 대한류마티스학회 2004 대한류마티스학회지 Vol.11 No.3

Behcet`s disease (BD) is a chronic inflammatory disease, involving several organs. It is well known that there are the marked regional differences in the disease expression of BD. In case of the vascular involvement in BD, the frequency rate has been described in 25∼30% among the patients from the Middle East, whereas it has been noted in 5∼15% in our country. In general, BD associated with large vessel lesions is named vasculo-BD. On the other hand, the cardiac involvement in BD has rarely been reported in the literature. Ebstein anomaly is a kind of malformation that is characterized by a downward displacement of the tricuspid valve into the right ventricle. We reported a 54-year-old male patient with vasculo-BD who had inferior vena cava obstruction and Ebstein anomaly. The association of Ebstein anomaly with vasculo-BD is considered to be coincidental. To the best of our knowledge, this is the first case of Ebstein anomaly associated with BD.

가용대역폭 관리를 통한 영상 스트리밍 서비스 품질 제어 시스템 설계 및 구현

이인선(In Sun Lee),김현종(Hyun Jong Kim),최성곤(Seong Gon Choi) 한국콘텐츠학회 2010 한국콘텐츠학회논문지 Vol.10 No.9

본 논문은 차세대 통합망 환경에서 IPTV와 같은 영상 스트리밍 서비스를 위해 종단간 가용대역폭 모니터링을 통하여 영상 품질을 제어할 수 있는 영상 품질 제어 시스템(VQCS)을 제안한다. IPTV 서비스를 통해 영상, 음성 및 게임과 같은 다양한 멀티미디어 서비스들을 제공할 수 있으며 이런 서비스들은 대용량의 대역폭을 소비하게 된다. 이때 네트워크 가용대역폭이 부족할 경우 영상 끊김, 형태 왜곡 및 잔상과 같은 영상 품질 저하가 발생하게 된다. 영상 스트리밍 서비스의 품질을 안정적으로 제어하기 위해서는 가용대역폭 모니터링 방안이 요구된다. 이에 우리는 단말 내의 영상 품질 제어 시스템에서 IP 헤더 내의 전체길이(Total Length) 필드를 이용하여 단위시간 동안 링크를 점유하고 있는 패킷 양 계산을 통해 가용 대역폭을 측정하였으며, 가용대역폭 정보를 기반으로 네트워크 내의 스케일러빌리티 추출기는 영상 스트림 데이터율을 적응적으로 선택하여 대역폭 부족으로 인한 영상 스트리밍의 품질 저하를 막아 서비스 품질을 향상시킬 수 있다. In this paper, we propose video quality control system(VQCS) which can control video service quality through the monitoring of end-to-end available bandwidth for video streaming service like IPTV in NGN convergence network. Various multimedia services such as video, voice and gaming service can be provided by IPTV, and these services require large amounts of bandwidth. At this time, video quality degradation like video jerkiness, block distortion and blurring is caused when network available bandwidth is insufficient. Available bandwidth monitoring method is need to stably control video streaming quality. So, we periodically calculate the amount of the packets in link and measure available bandwidth by using total length field in IP header at terminal. Scalability extractor in network selects suitable video streaming data rate based on available bandwidth and transports video streaming with adaptive data rate to prevent video quality deterioration.

경동맥 혈전 유발 동물모델을 이용한 한약재 추출물의 효능 검색

박인실 ( In Sil Park ),이기모 ( Ki Mo Lee ),이인선 ( In Sun Lee ),한재일 ( Jae Il Han ),전원경 ( Won Kyung Jeon ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.5

Objectives: The aim of this study was to examine anti-thrombotic effect of traditional herbal extracts in a rat model of ferric chloride (FeCl3)-induced carotid arterial thrombosis. Methods: Thirty minutes prior to a 35% FeCl3 application, Sprague Dawley(SD) rats were injected with the 10 types of traditional herbal extracts (100mg/kg, intraperitoneal injection), respectively. The effect of these herbal extracts was examined for time to occlusion(TTO) using the Laser doppler flow meter and measured for thrombus weight (TW) in FeCl3-induced thrombosis model. Results: In the TTO, Salvia miltiorrhiza (Sm, 2.30±0.28 min, p<0.001) and Santalum album (Sa, 2.19±0.19 min, p<0.001) showed significantly delayed TTO more than twice compared with Saline-treated group. Cnidium officinale (Co), Psoralea corylifolia (Pc), Scutellatia baicalensis (Sba), Panax notoginseng (Pn), Angelica tenuissima (At), Scrophularia buergeriana (Sbu), Rhus verniciflua (Rv) and Picrasma quassioides (Pq), except for Rhus verniciflua (Rv) also meaningfully impeded TTO more than one fold. In addition, Salvia miltiorrhiza, Santalum album, Cnidium officinale, Psoralea corylifolia and Scutellatia baicalensis significantly reduced TW more than 10% compared with Saline-treated group. Especially, Salvia miltiorrhiza and Santalum album showed the most excellent anti-thrombotic effect among the 10 herbal extracts tested on the restoration of altered TTO and TW. Conclusions: These results suggest that Sm and Sa extracts have outstanding anti-thrombotic effect in FeCl3-induced thrombosis model and is potentially useful as herbal medicines for the treatment and prevention of thrombosis.

초등학생 어머니의 양육스트레스, 대처방식 및 한방진단시스템과의 연관성 연구

임정화 ( Jung Hwa Lim ),이인선 ( In Sun Lee ),정인철 ( In Chul Jung ),황보민 ( Bo Min Hwang ),정민정 ( Min Jeong Jeong ) 대한한방신경정신과학회 2010 동의신경정신과학회지 Vol.21 No.4

Objectives: This study was to investigate the associations of Parent Stress Index(PSI), Way of Coping Checklist(WCC), and Diagnostic System of Oriental Medicine(DSOM) in mothers of elementary school children. Methods: In the study, K-PSI-SF, WCC, and DSOM were carried out on 202 mothers of ○○elementary school children during June, 2010. Cross tabulation analysis was used to verify the association of PSI, WCC and DSOM. Results: 1. The most common pathogenic factor was Dampness(濕) in total subjects. 2. The score of problem-focused coping methods showed significant difference in PSI grades. 3. The zp and sc10 of Qi deficiency(氣虛), Blood deficiency(血虛), Qi-Stagnation(氣滯), Insufficiency of Yang(陽虛), Heat(熱), Dampness(濕), Dryness(燥), Liver(肝), Heart(心), and Kidney(腎) showed significant difference in PSI grades. 4. The score of total PSI and PSI subscale had negative correlations with problem-focused coping methods. 5. The score of total PSI had positive correlations with Qi-Stagnation(氣滯), Dampness(濕), Dryness(燥), Heart(心), and Kidney(腎) in total subjects. Conclusions: This study provides insights on associations of parenting stress coping methods and diagnostic system in Oriental Medicine. Furthermore, the study shows positive correlations among Qi-Stagnation(氣滯), Dampness(濕), Dryness(燥), Heart(心), Kidney(腎) and PSI, and negative correlations between PSI and Problem-focused coping methods, all with statistical significance.

T-형 $Ca^{2+}$ 채널 길항제인 Mibefradil을 첨가한 인간 정자의 첨체반응 관찰

이재호,손원영,이정하,이인선,김영찬,한징택,Lee, Jae-Ho,Son, Weon-Young,Lee, Jung-Ha,Lee, In-Sun,Kim, Young-Chan,Han, Ching-Tack 대한생식의학회 2000 Clinical and Experimental Reproductive Medicine Vol.27 No.1

Objective: The sperm acrosome reaction is a $Ca^{2+}$-dependent exocytotic event that is triggered by adhesion to the mammalian egg's zona pellucida. Previous studies suggested a role of $Ca^{2+}$ channels in acrosome reactions. This study was conducted to investigate the T-type calcium channel is operated in acrosome reaction of human spermatozoa. Method: Human semen samples were obtained from healthy donors with normal criteria. The spermatozoa were divided into five groups: Group 1 were non-treated as a control; Group 2 where spermatozoa were exposed to 5 ${\mu}M$ $Ca^{2+}$ A23187 $(Ca^{2+}i)$; Group 3 where spermatozoa were exposed 5 ${\mu}M$ $Ca^{2+}i$ and mibefradil; Group 4 where spermatozoa were exposed 5 ${\mu}M$ $Ca^{2+}i$ and nifedipine, and Group 5 where spermatozoa were treated with 5 ${\mu}M$ $Ca^{2+}i$ and both of mibefradil and nifedipine. Spermatozoa in all groups were retrieved after incubation for 15 and 30 minutes at $37^{\circ}C$. After staining with PSA-FITC, fluorescence was observed under a fluorescence microscope, and AR was evaluated on a total>100 spermatozoa/side. Result and Conclusion: We observed on acrosome reaction inhibition rate in human spermatozoa the various of concentration of mibefradil, nifedipine. Maximum response was noted with 1.0 ${\mu}M$ mibefradil and the decrease of acrosome reaction inhibition rate 45%. Nifedipine in acrosome reaction inhibition rate was only about 25%. The $Ca^{2+}i$-induced AR of spermatozoa was significantly suppressed by mibefradil. Incidence of the suppression was depending on concentration of mibefradil. Results from the present study suggest that the human spermatozoa possess T-type channel. The observation that reversible inhibitor of T channels in male germ cells provides a new mechanism of contraceptive action.