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李王休 전북대학교 농업과학기술연구소 1991 農大論文集 Vol.22 No.1
Scanning-microscopic examination resulted that seed-treated bacteria became colonized with the rhizoplane of the sugar beets(Beta vulgaris L. var saccharifera) .The colonization showed remarkable differences among the bacterial strains. By testing bacteriological characteristics 10 bacterial strains were further selected from the 45 strains (Ann.Phytopath. Soc. Japan 52 175) of the net regions tested. Eight bacterial strains of them were identified as fluorescent pseudomonads and the rest were Bacillus sp. and Enterobacteriaceae respectively.
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생강 근경부패병의 발생 생태 및 토성 , 토양화학성 및 토양 생물성과의 관계
이왕휴,이두구 한국환경농학회 1998 한국환경농학회지 Vol.17 No.1
충남 서산과 전북 봉동지방의 생강 근경부패병의 발생생태를 조사하였다. 서산지방의 근경부패병 발병율은 봉동보다 낮았다. 서산의 생강 수확량은 평균 1,140㎏였고, 봉동은 818㎏/10a 였다. 발병후 살균제는 서산에서 많이 사용하였다. 포장간 토양물리성은 약간의 차이가 있었다. 발병율이 낮은 은하리는 23%의 점토를 함유하고 있다. 반면에 병발생이 많은 유소리는 미사함량이 60%이상이었다. 생강 재배 지역의 토양화학성은 적정 농도 범위였으나, 인산은 적정농도 450-500ppm(㎎/㎏)보다 모두 높았다. 이것은 하천의 오염원이 되므로 인산시비를 줄여야 할것을 추정하였다. 재배 중기인 8월에 사상균수가 최고치를 나타내는 경향이었고, 세균과 방선균수도 사상균수과 비슷한 경향이었다. 세균수/진균수는 포장간 큰차이를 나타냈다. The ecology of rhizome rot incidence rates between Seosan, Chungnam and Bongdong, Chonbuk province were surveyed in this experiment. The rhizome rot incidence rate of Seosan was lower than that of Bongdong. The average ginger product of former province was 1,140㎏, whereas it's 818㎏ per 10a. in latter province. Treatment after disease incidence frequently used fungicide at Seosan. There were different soil physiological property between fields. Although Eunhari and Yulsori were belong to Bongdong province, incidence rate was low at Eunhari, contained 23% clay and high incidence rate at Yulsori, approximately contained 60% silt. Many soil chemical properties of cultivated area were belong to optimum concentration range, but phosphate(P) was higher than optimum concentration, 450-550ppm. The number of soil fungi in the middle of cultivated season tended to show the highest. Both bacteria and actinomyces' number was similar to the result of fungi. The bacteria/fungi ratio was greatly different between surveyed fields.
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감자 역병균에 대한 스테롤류의 영향 및 감자절편에서의 난포자 형성
이왕휴,이용훈,이두구 한국자원식물학회 2001 한국자원식물학회지 Vol.14 No.1
역병균 (Phytophthora infestans)의 균사 신장 및 난포자 형성에 미치는 생리 화학적 영향을 알아보고자 국내에서 분리된 $A^2$형인 KM10, U6, MHB6, CDB6, JD1과 일본에서 분양 받은 $A^1$형인 F8l7, DNC303, $A^2$ 형인 IB908, DN107 균주를 이용하여 공시 배지별 및 V-8A 배지에 cholesterol, $\beta$-sitosterol및 lecithin을 첨가했을 때의 영향을 조사하고, 식물체상에서의 난포자 형성여부를 조사한 결과, 다음과 같은 결과를 얻을 수 있었다. 1. 균사 신장은 V-8A, RMA에서 양호하였고, 난포자 형성은 V-8A에서 가장 많았다. V-8A에 cholesterol, $\beta$-sitosterol 및 lecithin을 첨가하였을 때 접종 10일 후 균사 신장은 무처리 대비 각각 16.6, 8.3, 5.2% 더 신장하였으며, 난포자 형성수는 각각 76.0, 58.0, 34.6% 크게 증가하였다. 2. $A^1$형과 $A^2$형이 동시에 존재할 경우 식물체상에서 난포자를 형성하는지 알아보고자 식물체 조직편에 $A^1$, $A^2$형을 대치 배양하여 난포자 형성을 유도한 결과, 난포자의 형성률 및 수는 적었으나, 식물체 상에서도 난포자 형성이 가능한 것을 확인 할 수 있었는데, 금후 이에 대한 세심한 주의가 필요할 것으로 생각된다. The effects of media, cholesterol, $\beta$-sitosterol and lecithin on the growth and oospore production of the isolates KM10, U6, CDB6, MHB6, JD1 (A$^2$type) of Phytophthora infestans isolated in Korea and F8l7, DNC303 (A$^1$type), IB908, DN107 (A$^2$type) obtained from Japan were investigated. Mycelium of P. infestans grew better on V-8 juice agar and rye meal agar than on the other media. Oospores were produced most abundantly on V-8 juice agar. Mycelium extended more 16.6, 8.3, and 5.2% on V-8 juice agar supplemented with 5 $\mu\textrm{g}$/$m\ell$ of cholesterol, $\beta$-sitosterol and lecithin, respectively, and oospores are produced 76.0, 58.0, and 34.6 % on V-8juice agar supplemented with 5 $\mu\textrm{g}$/$m\ell$ of cholesterol, $\beta$-sitosterol and lecithin, respectively. Oospores more produced on detached potato plant disks when $A^1$ and $A^2$ type exist simultaneously which indicating that variation of population can occur in the field, but the rate of oospore formation and the number of oospores produced was low and small quantity.
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Paecilomyces japonica 인공 자실체 형성
이왕휴,최인영,최정식 한국균학회 1999 韓國菌學會誌 Vol.27 No.2
This study was conducted to investigate the morphological characteristics and cultural conditions for artificial fruiting body(synnemata) production of Paecilomyces japonica. In the morphological characteristics of P. japonica, the size of it's conidia was ranged from 5.0∼1.5 to 7.9∼2.4 ㎛. The artificial fruiting body showed yellow in color, shape was confirmed ellipsoidal or obovoid type, and the length was 50.6∼104.5 ㎜. The mycelial growth on the PDA medium treated with pH7, at 25℃ was superior to that of other treatments. The formation period of an artificial fruiting body of P. japonica treated with polypropylene and glass bottle culture was 30 days and 50 days, respectively. The length and number of fruiting body was longer and higher in the polypropylene bottle culture than those of the glass bottle culture. As the results, the artificial fruiting body production in the polypropylene bottle increased 1.2g per bottle compared to that of the glass bottle. It also increased in 100∼400 lx illumination, whereas the elongation of synnemata, pinheading and fruiting body growth were inhibited by continuous use of 900 lx illumination. The results of these experiment indicated that fruiting body formation seemed to be lower as the light intensity increased. The fruiting body formation was also dependent on the light color. There was a higher incidence in red color light and fluorescent light treatment than that of incandescent and blue color light. The fruiting body of the naked barley medium had so much better growth compared to other media that it would be able to use for it's production. The growth of fruiting body was affected by CO₂, concentration. It increased after putting the lid on the bottle.
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이왕휴,소인영 한국미생물학회 1983 미생물학회지 Vol.21 No.1
In order to study the effects of cropping system and fungicide (Dachigaren) on soil microbes, the seasonal fluctuations of soil microbes in the fields of radish and Chinese cabbage including soil pH, Soil moisture content and soil temperature were investigated on every 15 day interval from the begining of March to late October in 1981. The population of total fungus peaked at the begining of July, while that of total bacteria, at the begining of August. They were affected by soil temperature, however pathogenic microbes seemed to be more related with host plants than the soil temperature, because pathogens showed high density through the whole cultivation period. The pathogenic microbes showed the density of order ; Xanthomonas, Erwinia, Pseudomonas, Agrobacterium and Corynebacterium. Xanthomonas, Erwinia and Pseudomonas, which induced radish and Chinese cabbage diseases were higher than Agrobacterium and Corynebacterium in population densigy. Bacterial soft rot occured at the density of Erwinia $5.9{\sim}6.6{\times}10^5/dry$ soil 1 gram. The density of microbes on continuous fields were higher than that of rotating fields, but there were no significant difference between treated fungicide plot and non treated in the density of microbes, also no difference between Chinese cabbage and radish growing fields.
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李王休 전북대학교 농업과학기술연구소 1992 農大論文集 Vol.23 No.1
Twenty isolates of Phytophthora infestans from the foliage of blighted potatoes growing in the Chonbuk province, Korea were analyzed in 1991. All isolates were categorized as the At mating types by compatibility determination. There were significant differences in number of zoospores of plates among the fungal strairis. Mycellium growth on oat meal agar was more abundant than on 10% of V-8 juice cleared agar medium. The electrophoretic patterns at two enzyme loci, glucose Phosphate isomerase(GPI), and peptidase(PEP) resulted in mycellia from the isolates as A2: types. GPI had no less than two loci and two electromorphs at 100/100 and 157/157, whereas PEP had also two loci and two electromorphs at 93/93 and 59/59.
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