Euonymus alatus 추출물의 실험적 간 손상 억제

신숙정,이병용,신동근,이정호,Shin, Sook-Jeong,Lee, Byung-Yong,Shin, Dong-Keun,Lee, Jeong-Ho 대한면역학회 2001 Immune Network Vol.1 No.3

Background: A previous study has shown that Euonymus alatus (EA) has an antidotic activities against inflammation, suggesting possibility that EA can exert this beneficial effects to liver injury by an initial protection against drug-induced hepatocyte demage. The present study was undertaken to evaluate the protective effect of EA-extract on experimentally induced hepatitis in ICR mice and to investigate some mechanisms responsible for its action. Methods: Water EA extract was used in this experiments. The mice received i.p. a dose of 700 mg/kg galactosamine (GalN) together with $5{\mu}g/kg$ of endotoxin (LPS), or received i.v. 12 mg/kg of concanavalin A (Con A). EA (4 mg/mouse) was administrated on day -2, -1 and 0 before induction of liver injury. Liver injury was assessed by measurement of serum alanin amino-transferase (SGPT) levels on 9 hr after GaIN.LPS, or 8 hr after con A administration. Results: Treatment with either GaIN or LPS alone did not cause hepatitis. However, simultaneous administration of GalN and LPS to mice resulted in LPS-dose dependent fulminant hepatitis. GaLN/LPS-induced liver injury was reduced when mice were given EA for 3 days before induction. This preventive effect of Ea was more prominent when EA was given by intraperitoneal route rather then by oral route. Pretreatment of EA or dexamethasone inhibited significantly $TNF{\alpha}$ production in GalL/LPS-injured mice. However, EA-treatment did not influence $TNF{\alpha}$-induced hepatitis in GalN-sensitized mice, suggesting that $TNF{\alpha}$ is likely to act as one of final mediators of endotoxin action and the protective effect of EA might be manifested chiefly by inhibition of endotoxin-induced $TNF{\alpha}$ production, not by blocking the $TNF{\alpha}$-action. Injection of Con A into mice evoked remarkable liver injury in a dose dependent fashion. This liver damage was reduced by EA-pretreatment. Dexamethasone significantly reduced both GalL/LPS-induced and Con A-induced liver damages, showing synergism with EA. However, indomethacin reduced only GalN/ LPS-induced hepatitis, not for Con A-induced hepatitis. Conclusion: These results led to the conclusion that EA may be able to contribute at least in part to prevent the drug-induced hepatotoxicity, and that its anti-hepatitis effects might be manifested directly by modulation of endogenous mediators, such as leukotriese D4, $TNF{\alpha}$ and free radical, and indirectly by regulation of immune mediated responses. Also these results suggested that EA could be developed as a potential antidotic agent.

한국고대학회 2003년 춘계 학술회의 토론요지 : 토론 ; 한국 선사시대의 생업과 문화

하문식 ( Moon Sig Ha ),신숙정 ( In Soo Ha ),하인수 ( Sook Chung Shin ),이준정 ( June Jeong Lee ) 한국고대학회 2003 先史와 古代 Vol.18 No.-

누에-달맞이꽃 종자유 투여가 수컷 마우스의 성기능에 미치는 영향

양승옥 ( Seung Ok Yang ),박영경 ( Yong Kyeung Park ),신숙정 ( Sook Jeong Shin ),이정호 ( Jeong Ho Lee ) 전북대학교 의과학연구소 2003 全北醫大論文集 Vol.27 No.1

누에제품(SWP)이 정력증강제로서 시판되고 있으나 이가 과연 성기능 증강기능이 있는가는 아직까지 불명하다. 이에 본 실험은 이를 실험적으로 입증하고자 하였으며, 아울러, PGE가 발기에 관여하며 PGE의 전구물질이 다량 함유되어 있다고 알려진 달맞이꽃 종자유(EPO)가 성기능에 어더한 영향을 미치는가를 밝히기 위하여 계획되었다. 실험대상 동물은 ICR계 수컷마우스를 사용하였으며, 공시한 세포는 HUVE 세포와 마우스로부터 직접 적출한 음경해면조직의 평활근을 사용하였다. 마우스에의 SWP 및 EPO의 투여는 경구삽관에 의하여 1일 1회 14일간 투여하였다. 성기능 증강효과의 평가는 고환의 무게, 교미횟수, 혼숙시킨암 마우스 질외부의 정충양성율, 혈증 활성 testosterone의 농도, 음경 평활근의 PGE농도, 혈증 cGMP의 농도 그리고 혈관내피 세포의 NO생산능 등을 측정하여 실시하였다. 그 결과 다음과 같은 성정을 얻었다. 1. 시료의 투여에 의하여 체중이 대조에 비하여 다소 증가되었다. 2.고환의 무게는 시료 투여군에서 모두 증가되었는데 그 증가의 정도는 EPO 군보다 SWP 투여군에서 더욱 높았다. 3. 교미횟수는 대조에 비하여 SWP군에서는 다소 증가되었으나, EPO군에서는 현저히 증가되었다. 4. 정충 양성율은 시료 투여군 모두에서 대조군보다 약 2배 높았다. 5. 혈중 활성 testosterone의 농도는 EPO군에서는 2.3배 증가되었으나 SWP투여군과 대조군간에는 유의한 차이를 보이지않았다. 6. 음경평활근의 PGE생산능은 SWP에 의해서는 영향을 받지 않았지만 EPO군에 서는 유의하게 증가되었다. 7. 혈중 cGMP 농도는 시료 투여군 모두에서 증가되었다. 8. 혈관내피세포의 NO 생산능은 EPO에 의해서는 유의한 영향을 받지 않았지만 SWP으로 처리하면 유의하게 항진되었다. 이상의 결과는 SWP 및 EPO 모두 수컷마우스의 성기능을 NO, cGMP, PGE, 및활성 testosterone 생산 등 여러 경로를 통하여 증강시킴을 제시한다. Objectives: The present study was undertaken to evaluate the effect of silk worm powder (SWP) and evening primrose oil (EPO) on the male sexual functions such as increment testis weight, number of complete intromissions and mating, testosterone and cGMP levels in serum, prostaglandin levels of penile corpus cavernosum smooth muscle cells and NO-productive activity of endothelial cells. Methods: ICR male mice with same body weight and age were randomly divided into several experimental and control groups. SWP (daily 50 mg/mouse) or EPO (daily 0.5mg/mouse) was orally intubated for 28 consecutive days to experimental groups, and same vol. of vehicle to control group. The weight of body and testis were weighed at the time interval. On day 14, 30 minutes after dose, 2 virgin female mice were placed with 1 male mouse, and the number of complete intromissions of each male mouse in 3 hours was recorded. In an assessment of 1 day of mating, each male mouse was cohabited with 5 estrous female mice overnight, and then the number of sperm-positive females was recorded. Blood was obtained from mice on day 14 of dose, and serum concentrations of free testosterone and cGMP were measured by ELISA. On day 14, the penis was removed from each animals by cutting the crura corpus cavernosum, the corpus cavernosum were then excised and squeezed, and prostaglandin level of each sup was measured by ELISA. The HUVE cells were stimulated with ionomycin, and cultured for 48hrs in the presence of SWP and/or EPO, and then the level of NO in culture sup was measured. Results: The weight of body and testis of SWP and/or EPO treated mice was somewhat increased than that of control. The number of complete intromissions during the 3-hour period was 15.2 2.1, 23.2 2.5, 41.3 3.6, 37.1 2.9 for the control group, SWP group, EPO group, and SWP+EPO combined group, respectively. The number of sperm-positive females increased in experimental groups, as compared with that of control. The serum concentrations of testosterone was somewhat increased in SWP group and SWP+EPO group, however, was significantly elevated in EPO treated group. The releasing amounts of prostaglandin of penile corpus cavernosum was almost same levels in SWP-treated mice, but was markedly increased in EPO group. The serum cGMP level was significantly increased in all experimental groups. The NO production of ionomycin-stimulated HUVE cells was increased only when SWP was added into cultures. Conclusions: Oral administration of SWP and EPO enhanced the sexual function of male mice, as evidenced by an increase in the testis weight, number of complete intromissions and mating, serum testosterone and cGMP levels, productive activity of prostaglandin of penile smooth muscle cells, and NO production of endothelial cells. The present study suggests that both SWP and EPO can be developed as a tonic improving sexual function.

노부모에 대한 성인자녀의 양가감정에 영향을 미치는 요인

이신숙 ( Shin Sook Lee ),김서현 ( Seo Hyun Kim ) 한국가족복지학회 2014 한국가족복지학 Vol.19 No.3

This findings of this study can be applied to improve the relationships between aged parents and their adult children. The subjects were 552 adult children, and data analysis was performed using reliability, frequency, percentage, mean, standard deviation, one way ANOVA and multiple regression. The results of this study were as follows; 1) The degree of the elderly`s ambivalence of their adult children was 8.22(median: 15.5). The mean value in the importance of aged parents showed to be 2.22(median: 2). Meanwhile, the average of adult children`s depression was 21.3(median: 30). The overall role importance of adult children was higher than the median value of 3. 2) The elderly`s ambivalence level was significantly different according to the spouse, the relationship with the old parents, depression, the health condition of the old parents, the role importance of the worker, the importance of old parents, the economic status of the old parents, the role importance of the spouse, the economic status of the adult children, the monthly living expenses, the role importance of the parents, children, and face-to-face contact. 3) The variables that affect the elderly`s ambivalence level were the importance of the old parents, the role importance of the parents, the economic status of the old parents, education, the relationship with the old parents, the spouse, the health condition of the old parents, the health condition of the adult children, telephone contact, religion, face-to-face contact, the role importance of the spouse, and the economic status of the adult children, which explained about 45% of the total variance.

노인의 일상적 스트레스, 사회적 지지, 심리적 적응에 관한 연구

이신숙(Shin Sook Lee),이경주(Kyeong Ju Lee) 한국노년학회 2002 한국노년학 Vol.22 No.1

본 연구는 광주, 전남에 거주하는 60세 이상의 남녀노인 276명을 대상으로 노인의 일상적 스트레스, 사회적지지, 심리적 적용간의 관계를 살펴 보았다. 노인이 가장 많이 경험하는 스트레스 내용은 건강문제이고 스트레스 지각 정도가 큰 영역은 상실감이었다. 노인의 스트레스 정도는 비교적 낮게 나타난 반면에 사회적 지지는 높게 나타났다. 특히 자녀의 정서적 지지가 높게 나타났다. 배경변인에 따른 스트레스 지각 정도는 경제상태, 건강상태, 성별, 활동상태에 따라서 유의한 차이를 보였다. 사회적 지지에 있어서 먼저 자녀의 도구적지지는 자녀수, 거주형태, 성별, 경제상태에 따라 그리고 자녀의 정서적지지는 건강상태, 경제상태, 활동수준, 연령, 교육수준, 자녀수에 있어 집단 간에 의미있는 차이를 보였다. 한편 친구로부터 받는 도구적지지는 건강상태, 활동수준에 따라 그리고 정서적지지는 활동상태, 교육수준, 건강상태에 있어 집단 간에 의미있는 차이를 보였다. 노인들의 심리적 적용에 있어 우울감과 행복감은 종교유무 등의 일부 변인을 제외하고 거의 모든 변인에 있어 유의한 차이를 보였다. 노인의 심리적 적용에 영향을 미치는 상대적 영향력을 살펴본 결과, 우울감에는 스트레스 지각 정도가 가장 높게 영향을 끼쳤으며 건강상태, 자녀의 정서적지지, 경제상태, 성별, 활동상태 순으로 나타났으며 이들 변인의 설명력은 44%이었다. 행복감은 자녀의 정서적지지가 가장 높게 영향을 끼쳤으며 건강상태, 지각되는 스트레스 정도, 경제상태, 활동수준, 성별의 순으로 나타났고 이들 변인의 설명은 51%였다. The purpose of the study was to examine the trends of stress, social support, and psychological well-being and to present the influence of stress and social support on psychological well-being of the elderly. The subjects were 276 elderly man and woman living in Kwangju and Chonnam. Statics used such as Frequency, Percentage, Mean, Standard Deviation, Cronbach's α, T-test, Duncan test, ANOVA, and Stepwise Multiple Regression Analysis. The major results of this study can be summarized as follows; 1) The main stressors was health problem;, but they was most stress when they face loss of their acquaintance. 2) Overall the stress level of elderly was relatively low. but social support was high, especially emotional support received from the children was high. And depression of elderly was median, happiness of elderly was high. 3) There were meaningful differences in stress level according to economic status, health status, sex, activity level. There were also meaningful differences in the psychologically well-being(depression, happiness) according to most or the variables used in the analysis. 4) The variables which affect the elderly's stress level was economic status, health status, age that were explained about 18%. And it was shown that influencing variable on their depression was stress level. health status, emotional support of children, economic status, sex, activity level. Those variables stand 44% in the elderly's depression. And it was shown that influencing variable on their happiness was emotional support of children, health status, stress level, economic status, activity level, sex that were explained about 51%. In conclusion, Stress level and emotional support of children were very important variables on elderly's psychologically well-being.

유ㆍ무배우 여자노인의 연령, 경제상태, 신체만족도에 따른 생활만족도

이신숙(Lee Shin-Sook) 가정과삶의질학회 2010 가정과삶의질연구 Vol.27 No.6

노인의 성생활 인식이 부부 간의 친밀감과 주관적 행복감에 미치는 영향

이신숙 ( Lee Shin Sook ),이선정 ( Lee Seon Jeong ) 한국가족관계학회 2019 한국가족관계학회지 Vol.23 No.4

Objectives: The purpose of this study was to investigate whether the perceptions of older adults’ sexual life was associated with their marital intimacy and subjective happiness. Method: The subjects were 247 elderly men and women aged over 60 years living in K, S, and Y city located in Jeonnam were collected. Statistical analysis were reliability, frequency, percentage, mean, standard deviation, ANOVA, correlation and stepwise multiple regression analysis. Results: The major results of this study were as below. First, the average perceived sexuality of older adults was lower than that of the median, and the perceived sex of older adults was relatively low. However, intimacy and happiness scores between couples are higher than the median, suggesting a high level of intimacy and happiness. Second, the perceived degree of older adults sex life was different among sex, monthly income, education, career, age, health status, economic status, house, residence and religion. Third, there was a close relationship between perceived sexuality of elderly, intimacy between couples, and subjective happiness. Fourth, the relative influence of the variables on the happiness of older adults showed 45% of the total influence power, followed by the health status, the respect of the spouse, the importance of sex life, need for sex life, house, religion, education. Conclusions: The results of this study show that the perception of older adults sex life affects the intimacy and subjective well-being of the couple.

중학생의 학업적 꾸물거림과 학업성취도에 관한 실증적 인과관계 분석

이신숙(Shin-Sook LEE) 한국컴퓨터정보학회 2015 韓國컴퓨터情報學會論文誌 Vol.20 No.6

본 연구는 중학생의 학업적 꾸물거림이 학업성취도에 미치는 영향력을 밝히고자 하였다. 또한 중학생이 인지하는 스트레스 정도가 학업성취도에 미치는 영향력이 있는지를 분석하였다. 먼저 이론적 고찰과 선행연구를 검토하여 중학생의 학업적 꾸물거림의 하위요인인 행동적 요인, 인지적 요인, 정서적 요인이 학업성취도에 영향을 미치는 것으로 가정하였다. 또한 중학생의 스트레스가 학업성취도에 미치는 영향력이 있는 것으로 가정하였다. 연구결과 중학생의 학업적 꾸물거림의 하위요인인 행동적 요인, 인지적 요인, 정서적 요인이 스트레스와 학업성취도에 부분적으로 직접적인 영향력이 미치는 것으로 나타났다. 중학생의 스트레스는 학업성취도에 미치는 영향력이 없는 것으로 나타났다. 본 연구는 규명된 연구결과를 바탕으로 중학생의 학업성취도에 대한 정책적 시사점을 대안으로 제시하였다. This study aims to examine the effects of academic procrastination in middle school students on their academic achievement. It also analyzed the influences of stress level recognized by middle school students on their academic achievement. First, it examined theoretical and preceding studies and assumed that sub-factors of academic procrastination in middle school students such as behavioral, cognitive and emotional factors affected their academic achievement. It was assumed that the middle school students’ stress had the direct mediating effects on their academic achievement. As a result of the research, it was found that sub-factors of academic procrastination in middle school students such as behavioral, cognitive and emotional factors had partially direct influence on their stress and academic achievement. It was shown that the analysis of mediating effects from middle school students’ stress had no influence on the academic achievement. This study presented policy suggestions of academic achievement in middle school students as an alternative based on the research results.

남녀 노인의 경제상태 , 건강상태 , 성역할 정체감에 따른 자아존중감

이신숙(Shin Sook Lee),이경주(Kyeong Ju Lee) 한국가정과학회 2001 한국가정과학회지 Vol.4 No.1

The purpose of the study was to investigate relationships among self-esteem of the elderly men and women in the economic state, health state, and degree of sex role identity. The subjects were 246 elderly men and women living in Chonnam. Statistics were Frequency, Percentage, Mean, Standard Deviation, Cronbach`s a, ANOVA and Regression analysis. Major results obtained were as follows: 1) The mean scores of self-esteem in the elderly men and women were 19.26 and 18.84 respectively. 2) There were meaningful differences in self-esteem according to health state, economic state, sex role identity in both elderly men and women. And, in the elderly men and Women, the highest influencing variables on their self-esteem were the economic state and health state, and the sex role identity respectively.

한ㆍ미 대학생의 노인과 노화에 대한 태도가 조모-손자녀 간의 친밀도에 미치는 영향

이신숙(Lee Shin-Sook) 가정과삶의질학회 2006 가정과삶의질연구 Vol.24 No.5