F-67 : Free Paper Presentation ; Depletion of Platelets Enhances Therapeutic Antitumor Effects Generated by Therapeutic DNA Vaccine

이승룡,심재겸,최종현,이승헌,민경훈,이은주,허규영,이상엽,김제형,신철,심재정,인광호,강경호 대한결핵 및 호흡기학회 2013 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.116 No.-

It has been well established that platelets are associated with tumor progression and metastasis as they can support angiogenesis and prevent hemorrhage. As such, platelets may be an ideal target for enhancing anticancer immunotherapies. Thus, we hypothesized that inducing thrombocytopenia would increase the leakiness of tumor vasculature thereby facilitating the effective delivery of antigen- specific T cells generated by DNA vaccination to tumor loci in order to induce CD8+ T cell-mediated tumor killing. We have previously developed a therapeutic HPV DNA vaccine encoding calreticulin (CRT) linked to HPV-16 E7 antigen (CRT/E7) to enhance therapeutic DNA vaccine potency. In the current study, we explore the employment of a platelet-depleting antibody or heparin in combination with CRT/E7 DNA vaccine in a mouse model for its potential to enhance E7-specific CD8+ T cell immune responses as well as antitumor effects against E7-expressing tumors. We induced severe acute thrombocytopenia in mice bearing subcutaneous TC-1 tumors. TC-1 tumor cells originate from the mouse lung and express the HPV-16 E7 tumor-specific antigen. In a TC-1 mouse tumor model, platelet depletion increased E7-specific CD8+ T cells in the peripheral blood and reduced the growth of the tumor mass following CRT/E7 DNA vaccination. Next, we isolated platelet-rich plasma from tumor-bearing and naïve mice. When TC-1 tumor cells were coincubated with E7-specific CD8+ T cells under platelet-rich plasma and found that E7-specific CD8+ T cells had decreased IFN-γ secretion in the presence of platelet-rich plasma from tumor-bearing mice compared to that of naïve mice. Furthermore, when incubated with E7-specific CD8+ T cells and platelets, TC-1 cells were killed significantly less efficiently compared to those incubated with E7-specific CD8+ T cells alone. Importantly, we found that GFP-tagged TC-1 cells incubated with platelets were coated by the platelets, which were subsequently activated (CD26P+). Taken together, these data suggest that platelet depletion increases tumor-specific CD8+ T cells and enhances their tumor killing effects. Furthermore, This study provides proof in principle that platelets directly inhibit CD8+ T cells from killing tumor cells. In conclusion, platelet depletion or heparin therapy can stimulate antitumor immune responses and this treatment methodology may be applicable to a variety of cancers.It has been well established that platelets are associated with tumor progression and metastasis as they can support angiogenesis and prevent hemorrhage. As such, platelets may be an ideal target for enhancing anticancer immunotherapies. Thus, we hypothesized that inducing thrombocytopenia would increase the leakiness of tumor vasculature thereby facilitating the effective delivery of antigen- specific T cells generated by DNA vaccination to tumor loci in order to induce CD8+ T cell-mediated tumor killing. We have previously developed a therapeutic HPV DNA vaccine encoding calreticulin (CRT) linked to HPV-16 E7 antigen (CRT/E7) to enhance therapeutic DNA vaccine potency. In the current study, we explore the employment of a platelet-depleting antibody or heparin in combination with CRT/E7 DNA vaccine in a mouse model for its potential to enhance E7-specific CD8+ T cell immune responses as well as antitumor effects against E7-expressing tumors. We induced severe acute thrombocytopenia in mice bearing subcutaneous TC-1 tumors. TC-1 tumor cells originate from the mouse lung and express the HPV-16 E7 tumor-specific antigen. In a TC-1 mouse tumor model, platelet depletion increased E7-specific CD8+ T cells in the peripheral blood and reduced the growth of the tumor mass following CRT/E7 DNA vaccination. Next, we isolated platelet-rich plasma from tumor-bearing and naïve mice. When TC-1 tumor cells were coincubated with E7-specific CD8+ T cells under platelet-rich plasma and found that E7-specific CD8+ T cells had decreased IFN-γ secretion in the presence of platelet-rich plasma from tumor-bearing mice compared to that of naïve mice. Furthermore, when incubated with E7-specific CD8+ T cells and platelets, TC-1 cells were killed significantly less efficiently compared to those incubated with E7-specific CD8+ T cells alone. Importantly, we found that GFP-tagged TC-1 cells incubated with platelets were coated by the platelets, which were subsequently activated (CD26P+). Taken together, these data suggest that platelet depletion increases tumor-specific CD8+ T cells and enhances their tumor killing effects. Furthermore, This study provides proof in principle that platelets directly inhibit CD8+ T cells from killing tumor cells. In conclusion, platelet depletion or heparin therapy can stimulate antitumor immune responses and this treatment methodology may be applicable to a variety of cancers.

통합 스트리밍 서비스 구조 설계 및 구현

이승룡,홍충선 한국통신학회 2001 韓國通信學會論文誌 Vol.26 No.6

본 논문에서는 멀티미디어 스트리밍을 위한 표준 프로토콜과 미디어 포맷을 지원하고, 실시간 객체지향형 멀티미디어 데이터베이스(BeeHive)를 지원하는 통합 멀티미디어 스트리밍 서비스 프레임워크(ISSA)를 제안한다. ISSA의 핵심 구성요소는 세션의 생성과 스트림 제어를 담당하는 세션 관리자, 스트리밍 데이터의 전송을 담당하는 전송 관리자, 미디어의 압축과 복원 및 재생을 담당하는 미디어 관리자, 그리고 데이터베이스와의 연동을 담당하는 데이터베이스 커넥터이다. ISSA는 스트림의 제어를 위하여 IETF에서 제안한 RTSP, 스트림과 세션의 표현과 초기화를 위해 SDP와 SIP, 스트림 데이터의 실시간 전송을 위하여 RTP 및 TCP, UDP를 지원한다. 또한, ISSA는 VOD, MOD, 웹캐스팅을 위한 개발과 라이브러리를 제공하며, 웹서버와 CORBA A/V 스트리밍 서비스와의 연동을 위한 게이트웨이도 제공한다. ISSA는 운영체제 환경에서 플랫폼에 독립적으로 작동하고, 객체지향 멀티미디어 데이터베이스 서비스를 지원한다. 그리고 멀티캐스팅, 웹캐스팅, 멀티미디어 통신을 위한 표준 프로토콜을 지원하며, 상용 코덱인 Microsoft 사의 Direct Show를 지원할 수 있는 RTP 소스필터를 가지고 있다. 마지막으로, 기존의 다양한 표준 미디어 포맷을 지원할 뿐만 아니라, 새로운 미디어 포맷도 쉽게 수용할 수 있도록 설계되어 있다.

The Different Expression of Regulatory T Cells and Th17 Cells in the Sentinel Lymph Nodes (SLNs) and Lung Cancer Tissue (초)

이승룡,최혜경,이승현,문재영,김혜옥,이은주,허규영,정기환,이상엽,김제형,신철,심재정,인광호,강경호,유세화,김현구 대한결핵 및 호흡기학회 2010 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.110 No.-

고립 상장간막동맥박리로 스텐트 삽입 후 발생한 상장간막 동맥증후군

이승룡,김성훈,남상우,김인희,김상욱,이승옥,이수택 대한내과학회 2011 대한내과학회지 Vol.80 No.-

상장간막동맥증후군은 대동맥과 상장간막동맥이 이루는 각이 줄어들면서 십이지장의 폐쇄를 유발하는 매우 드문 질환이다. 여러 의학적 혹은 정신적 질환에 의해 급격한 체중 감소가 발생하면 십이지장 제3부가 통과하는 대동맥과 상장간막동맥 간의 공간에 지방조직이 줄어서 발생하게 된다. 고립 상장간막동맥박리는 급성 장간막 허혈의 매우 드문 원인 중 하나인데, 장경색을 유발하거나 상장간막동맥의 파열의 위험이 높은 박리증의 치료는 응급수술이 우선이고, 생체활력징후가 정상이고, 증상이 심하지 않은 경우엔 보존적인 치료를 시행할 수 있다고 알려져 있다. 최근 몇몇 문헌에서 고립 상장간막동맥박리 때 경피 혈관 스텐트삽입술로 장경색으로의 진행을 막고 단기 예후가 좋다고 보고하고 있으나, 장기 예후에 관한 보고가 없고, 스텐트 관련 합병증이 보고된 것이 적어서, 혈관 스텐트삽입술의 안정성이 명확하게 밝혀지지 않은 상황이다. 본 저자들은 고립 상장간막동맥박리로 인한 급성 장간막 허혈로 경피 혈관 삽입술을 시행 후 발생한 상장간막동맥증후군 합병증을 치료한 증례를 최초로 경험하여 보고하는 바이다. Superior mesenteric artery (SMA) syndrome is a rare disorder, characterized by a decreased aortomesenteric angle that causes a duodenal obstruction. Various medical and psychiatric conditions may result in the initial rapid weight loss that causes narrowing of the aortomesenteric angle. Isolated dissection of the superior mesenteric artery (DSMA) is an uncommon cause of acute mesenteric ischemia. Several literature reports suggest that medical treatment in combination with close observation is reasonable in uncomplicated dissection with stable hemodynamic status. Recently, as several reports describe, rapid revascularization by percutaneous angioplasty and endovascular stent insertion can prevent bowel ischemia that progresses to irreversible bowel necrosis. However, the stability of endovascular stents at the DSMA has not been confirmed, because its use is reasonably infrequent. Here, we report a case of SMA syndrome, as a complication of endovascular stent placement at the DSMA. (Korean J Med 2011;80:S67-S72)

크리스탈 볼룸 1 : 일반연제 발표 ; 기도 폐쇄를 동반한 진행성 폐암환자 및 수술이 곤란한 카르시노이드종양 환자에서 냉동 치료의 효과 및 부작용

이승룡,문재영,김혜옥,이경주,정진용,하은실,김정하,이은주,강은해,정기환,김제형,신철,심재정,인광호,유세화,강경호 대한결핵 및 호흡기학회 2007 대한결핵 및 호흡기학회 추계학술대회 초록집 Vol.105 No.-

포스터 전시 : 간경변증에서 잔존 간기능을 나타내는 지표: 아포지단백 A-1

이승룡,이호경,권혁진,문병식,이승옥,김대곤 대한간학회 2003 Clinical and Molecular Hepatology(대한간학회지) Vol.9 No.3(S)

Background/Aims: Apolipoprotein A-I(Apo A-I) is synthesized mainly by hepatocytes. It is decreased in alcoholic patients with liver fibrosis and cirrhosis. The mechanisms of serum apo A-I decrease in liver cirrhosis are unknown, but it has been shown that serum apo A-I measurement alone or in correlation with other biological data such as prothrombin time and r-glutamyl transferase, albumin and total bilirubin can serve as a marker of liver cirrhosis in alcoholic patients. The goal of this study was to to describe the changes of apo A-I according to the different stages of the liver cirrhosis, to correlate the changes to serum liver tests and to estimate its diagnosis, prognotic valuse and functional reserve of injuried liver. Mathods: We examed the value of apo A-I in 201 patients of liver cirrhosis and 138 patients of hepatocellular carcinoma in Korea. Results: The results showed that apo A-I concentration is highly related to the degree of liver injury, reaching a minimum in patients with severe cirrhosis. And apo A-I is correlated with biological marker of liver function such as albumin, prothrombin time, total bilirubin, α- macroglobulin. Apo A-I had an independent and discriminative value for the diagnosis of cirrhosis. This result was also observed not only in patients of alcoholic liver cirrhosis but also in patients of hepatocellular carcinoma due to hepatitis B. Conclusions: Our results suggest that serum Apo A-I is index for functional reserve in the irreversible liver disease such as cirrhosis of liver and hepatocellular carcinoma.

유도철선과 Stiffener를 이용한 기능부전 복막투석 카테터의 투시하 교정

이승룡 대한영상의학회 1997 대한영상의학회지 Vol.37 No.5

Purpose : To determine the efficacy of correction of a malfunctioning peritoneal dialysis catheter withguidewire and stiffener under fluoroscopic guidance. Materials and Method : Between November 1994 and March 1997,we performed 15 manipulations in 12 patients in whom a dual-cuff, straight Tenckhoff peritoneal dialysis catheterhad been implanted due to chronic renal failure. The causes of catheter malfunctioning were inadequate drainage ofthe dialysate(n=14) and painful dialysis(n=1). Under fluoroscopic guidance, adhesiolysis and repositioning of themalfunctioning catheter were performed with an Amplatz Super Stiff guidewire and the stiffener from a biliarydrainage catheter. The results of procedures were categorized as either immediate or durable success, this latterbeing defined as adequate catheter function for at least one month after the procedure. Results : Immediatesuccess was achieved in 14 of 15 procedures (93%), and durable success in 7 of 15 (47%). The mean duration ofcatheter function was 157 (range, 30 to 578) days. After manipulation, abdominal pain developed in eight patientsand peritonitis in two, but with conservative treatment, these symptoms improved. Conclusion : The correction of amalfunctioning peritoneal dialysis catheter with guidewire and stiffener under fluoroscopic guidance is aneffective means of restoring catheter function and may be an effective alternative to surgical reimplantation ofthe catheter, or hemodialysis.

골육종의 흉곽내 전이형태

이승룡,정규식,오경승,고지호,허진도,조영덕 고신대학교 의학부 2004 高神大學校 醫學部 論文集 Vol.19 No.1

The purpose of this study is to evaluate findings of altered patterns of thoracic metastasis in patients treated for osteosarcoma with adjuvant chemotheapy. The author reviewed medical records and image findings of 60 osteosarcoma patients from 1991 to 1997. 18 patients had thoracic metastasis confirmed by needle biopsy(n=1) and clinical evidence of tumor progression(n=17). CT was available in 17 patients. In 6 patients, mean 3 times serial CT were obtained during follow-up period. Two radiologists retrospectively reviewed the CT findings of parenchymal lesion, pleural lesion, mediastinal lesion, lymphadenopathy, calcification, and the follow-up change. In 16 cases, single or multiple nodules, suggesting typical appearance of hematogeneous metastasis, were present. Pleural plaque or mass was present in seven cases. In two of these cases, small curvilinear calcification or calcific dot was noted along the pleura, initially. On serial follow up CT scans, these calcifications was gradually increased in size, which resulted in huge calcified pleural mass. One patient showed only diffuse pleural thickening without other involvement, which proved pleural metastasis of osteosarcoma by means of pleural biopsy. Linear calcification or calcified nodule along the bronchial wall was noted in three cases this finding gradually changed to huge calcified mass with associated atelectasis of lung parenchyma. Other sites of metastasis were pericardium, 3 patients, and lymph node, 4 patients. Interlobular septal thickening suggesting lymphangitic metastasis was noted in 2 patients. Calcification within parenchymal nodule and metastatic lymph node was seen in 7 patients. Pneumothorax was noted in 2 patients. Interestingly, parenchymal lesion was slowly increased in size and number, while pleural and endobronchial lesion revealed rapid change and affressive invasion into the parenchyma, mediastinum and chest wall on follow-up CT scans. Thoracic metastasis of osteosarcoma commonly presents hematogenous parenchymal nodules. However, adjuvant chemotherapy has resulted in prolonged survival, and may raised the unusual appearance of thoracic metastasis, which is more aggressive nature. We carefully concluded that any type of calcification should be suspected metastasis in osteosarcoma patient.

통일교에서 바라본 9.11테러 해결방안 연구

이승룡 선문대학교 신학대학 2001 선문대학교 신학대학논문집 Vol.2001 No.-

세계의 중심도시이자 경제의 중심인 뉴욕의 맨하탄, 110층의 세계무역센타가 마치 영화의 한 장면처럼 무너져 내리고 말았다. 무너져 내릴 때 건물만 무너져 내렸으면 얼마나 좋았을까만은, 그 무너지는 건물 안에는 수많은 무고한 시민들도 처절히 절규하며 함께 무너져 내리고 말았다. 왜 이시대에 이런 아픔을 겪어야만 하는가… 이에 새롭게 외치는 Service for Peace가 절망에 빠진 인류를 구할 수 있는 해답이 될 것이며 그렇게 만들어야 할 것이다. 하나님을 부모로 모신 가운데 전 인류는 한 가족일 수 밖에 없음을 일깨워주고, 참사랑의 공동체를 형성해 나가야 되기 때문이다.

농업용 저수지의 효율적 활용방안 연구 - 경기남부권 농업용 저수지를 중심으로

이승룡,박수훈,장준호 더나은도시디자인포럼 2023 도시디자인저널 Vol.- No.5

This study aims to find a recycling method for agricultural reservoirs that have lost or deteriorated as agricultural reservoirs. The method of research is to define functional reservoirs for agriculture through preceding studies, case studies, current status surveys, and surveys, and to derive usage plans for each type. The problems of functional loss agricultural reservoirs were found in economic/industrial, safety, developmental, and socio-cultural aspects in previous studies. As a result of the survey, damage to the urban landscape emerged as the biggest problem. Through previous studies and case studies, it was identified that type of hydrophilicity, leisure type, energy development type, types of historical and cultural resources linkage, ecological learning type, etc. are being used. The need for utilization measures has been raised. In order to suggest usage plans for each type, five types were classified based on the size of the agricultural reservoir, the surrounding population, and the presence or absence of ecological and natural and historical resources of the adjacent area. As a result of classification, it was found that the ecological learning type, type C, took up the largest proportion with 10 sites, and the type D, types of historical and cultural resources linkage, occupies the smallest proportion. The result is meaningful in that it defines and classifies the types of loss-of-function agricultural reservoirs in the southern Gyeonggi region, and suggests usage plans for each type.