Anti-Inflammatory Effect for Atherosclerosis Progression by Sodium-Glucose Cotransporter 2 (SGLT-2) Inhibitor in a Normoglycemic Rabbit Model

이슬기,Seung-Jun Lee,Jung-Jae Lee,Jung-SunKim,Oh-Hyun Lee,김충기,Darae Kim,Yong-Ho Lee,JaeWonOh,Se Il Park,전옥희,Sung-Jin Hong,Chul-Min Ahn,Byeong-Keuk Kim,Young-Guk Ko,Donghoon Choi,Myeong-Ki Hong,Yangsoo Ja 대한심장학회 2020 Korean Circulation Journal Vol.50 No.5

Background and Objectives: We sought to investigate an anti-atherosclerotic and anti-inflammatory effect of sodium-glucose cotransporter-2 (SGLT-2) inhibitors in normoglycemic atherosclerotic rabbit model. Methods: Male New Zealand white rabbits (n=26) were fed with a 1% high-cholesterol diet for 7 weeks followed by normal diet for 2 weeks. After balloon catheter injury, the rabbits were administered with the Dapagliflozin (1mg/kg/day) or control-medium for 8 weeks (n=13 for each group). All lesions were assessed with angiography, optical coherence tomography (OCT), and histological assessment. Results: Atheroma burden (38.51±3.16% vs. 21.91±1.22%, p<0.01) and lipid accumulation (18.90±3.63% vs. 10.20±2.03%, p=0.047) was significantly decreased by SGLT-2 inhibitor treatment. The SGLT-2 inhibitor group showed lower macrophage infiltration (20.23±1.89% vs. 12.72±1.95%, p=0.01) as well as tumor necrosis factor (TNF)-α expression (31.17±4.40% vs. 19.47±2.10%, p=0.025). Relative area of inducible nitric oxide synthase+ macrophages was tended to be lower in the SGLT-2 inhibitor-treated group (1.00±0.16% vs. 0.71±0.10%, p=0.13), while relative proportion of Arg1+ macrophage was markedly increased (1.00±0.27% vs. 2.43±0.64%, p=0.04). As a result, progression of atherosclerosis was markedly attenuated in SGLT-2 inhibitor treated group (OCT area stenosis, 32.13±1.20% vs. 22.77±0.88%, p<0.01). Mechanistically, SGLT-2 treatment mitigated the inflammatory responses in macrophage. Especially, Toll-like receptor 4/nuclear factor-kappa B signaling pathway, and their downstream effectors such as interleukin-6 and TNF-α were markedly suppressed by SGLT-2 inhibitor treatment. Conclusions: These results together suggest that SGLT-2 inhibitor exerts an anti-atherosclerotic effect through favorable modulation of inflammatory response as well as macrophage characteristics in non-diabetic situation.

유전체 시대에 반수체 육종의 재발견

이슬기,김정선,강상호,손성한,원소윤,Lee, Seulki,Kim, Jung Sun,Kang, Sang-Ho,Sohn, Seong-Han,Won, So Youn 한국식물생명공학회 2016 식물생명공학회지 Vol.43 No.1

DNA 염기서열 분석기술의 진보는 많은 근본적인 생명현상을 이해하는데 기여해왔다. 유례없는 저비용에 염기서열을 대량으로 분석을 할 수 있게 되어 단일 규모의 실험실에서도 관심이 있는 종의 신규유전체를 해독할 수 있다. 게다가 유전집단의 전체 염기서열을 편향되지 않은 채 분석하여 무수한 분자마커를 발굴할 수 있게 됨에 따라 집단유전학 연구도 두드러지게 가속화되어 왔다. 그러나 식물의 유전체가 이형접합성, 반복염기서열, 배수성과 같은 복잡한 특성이 있다는 것을 고려해 볼 때 기술이 매우 빠르게 진화함에 따라 적절한 개체 혹은 집단을 확보하는 것이 식물 연구에서 주요한 문제가 되었다. 이러한 난제는 오래되었지만 매우 효율적인 기술인 반수체 육성을 통하여 극복될 수 있을 것이다. 정상적인 개체가 갖는 염색체의 절반을 보유하는 반수체 식물은 주로 자방이나 화분과 같은 배우체 세포를 배양함으로써 빠르게 구축될 수 있다. 뒤이은 반수체 식물의 염색체 배수화는 완벽한 동형접합성을 보이는 안정된 배가반수체를 만든다. 본 논문에서는 반수체 식물을 육성하고 판별하기 위한 고전적인 방법론을 요약할 것이다. 게다가 동원체의 히스톤을 후성적으로 조절함으로써 반수체를 유도하는 방법을 설명할 것이다. 마지막으로, 유전체 시대에 반수체 식물의 활용 방안을 유전체 해독과 집단 유전학의 측면에서 논의할 것이다. Advances in DNA sequencing technologies have contributed to revolutionary understanding of many fundamental biological processes. With unprecedented cost-effective and high-throughput sequencing, a single laboratory can afford to de novo sequence the whole genome for species of interest. In addition, population genetic studies have been remarkably accelerated by numerous molecular markers identified from unbiased genome-wide sequences of population samples. As sequencing technologies have evolved very rapidly, acquiring appropriate individual plants or populations is a major bottleneck in plant research considering the complex nature of plant genome, such as heterozygosity, repetitiveness, and polyploidy. This challenge could be overcome by the old but effective method known as haploid induction. Haploid plants containing half of their sporophytic chromosomes can be rapidly generated mainly by culturing gametophytic cells such as ovules or pollens. Subsequent chromosome doubling in haploid plants can generate stable doubled haploid (DH) with perfect homozygosity. Here, classical methodology to generate and identify haploid plants or DH are summarized. In addition, haploid induction by epigenetic regulation of centromeric histone is explained. Furthermore, the utilization of haploid plant in the genomics era is discussed in the aspect of genome sequencing project and population genetic studies.

Genome-Wide Screen of Human Bromodomain-Containing Proteins Identifies Cecr2 as a Novel DNA Damage Response Protein

이슬기,권종범,박은정,이한새,Ye Seul Lee 한국분자세포생물학회 2012 Molecules and cells Vol.34 No.1

The formation of -H2AX foci after DNA double strand breaks (DSBs) is crucial for the cellular response to this lethal DNA damage. We previously have shown that BRG1, a chromatin remodeling enzyme, facilitates DSB repair by stimulating -H2AX formation, and this function of BRG1 requires the binding of BRGI to acetylated histone H3 on -H2AX-containing nucleosomes using its bromodomain (BRD), a protein module that specifically recognizes acetyl-Lys moieties. We also have shown that the BRD of BRG1, when ectopically expressed in cells, functions as a dominant negative inhibitor of the BRG1 activity to stimulate -H2AX and DSB repair. Here, we found that BRDs from a select group of proteins have no such activity, suggesting that the -H2AX inhibition activity of BRG1 BRD is specific. This finding led us to search for more BRDs that exhibit -H2AX inhibition activity in the hope of finding additional BRD-containing proteins involved in DNA damage responses. We screened a total of 52 individual BRDs present in 38 human BRD-containing proteins, comprising 93% of all human BRDs. We identified the BRD of cat eye syndrome chromosome region candidate 2 (Cecr2), which recently was shown to form a novel chromatin remodeling complex with unknown cellular functions, as having a strong -H2AX inhibition activity. This activity of Cecr2 BRD is specific because it depends on the chromatin binding affinity of Cecr2 BRD. Small interfering RNA knockdown experiments showed that Cecr2 is important for -H2AX formation and DSB repair. Therefore, our genome-wide screen identifies Cecr2 as a novel DNA damage response protein.

밭 비점오염저감을 위한 잔디초생대 적용 비용 및 활용성 평가

이슬기,장정렬,최경숙,Lee, Seul-Gi,Jang, Jeong-Ryeol,Choi, Kyung-Sook 한국농공학회 2015 한국농공학회논문집 Vol.57 No.2

This study was aimed to estimate the total application cost and utilization of Turf grass VFS application through the field experiment. The experimental plots were constructed in an upland field of Iksan city within the Saemangeum watershed. Turf grass was transplanted at the down-slope edge of the pollution source area in each of the treated plots. Three rainfall events were monitored during the experiment period, and the rainfall-runoff relationships and NPS pollution reduction effects of the VFS systems were assessed. As results, the reduction ratio of runoff volume ranged 14.1~64.0 %, while the NPS pollution reduction ratio ranged 9.8~73.9 % for SS, 24.0~84.2 % for T-N, 31.6~80.9 % for T-P respectively. The total cost of VFS application was estimated by considering purchase cost of Turf grass sods and construction and maintenance costs of VFS system as well as the loss caused by giving up crop cultivation for the area needed to construct the VFS. The total cost of the VFS was estimated to be approximately \3,379,000/ha/year for the first year of application, and this cost could be decreased to \1,899,000/ha/year from the second year as the construction cost of VFS could no longer need to be counted afterwards. Apart from the NPS pollution reduction effects, the possible utilization of VFS was examined by detaching Turf grass within 40 % of VFS area for sale during spring time when the VFS systems fully covered. The benefit of selling the detached Turf grass sods was estimated as \1,260,000/ha/year, and also found that the VFS area successfully recovered by the time of the summer period. This benefit could attract farmers to adopt the VFS technique to manage agricultural NPS pollution.

새만금유역 밭경지 초생대 유사저감효과 모의

이슬기,장정렬,최경숙,Lee, Seul Gi,Jang, Jeong Ryeol,Choi, Kyung Sook 한국수자원학회 2018 한국수자원학회논문집 Vol.51 No.6

본 연구에서는 VFSMOD-W 모형을 활용하여 새만금 유역의 초생대 기법 적용에 따른 유사저감효과를 예측하고자 하였다. 먼저 모형의 신뢰도 확보를 위해 새만금 유역 내 초생대 실측치를 활용하여 매개변수를 보정하였으며, 새만금 유역 내 밭경지 특성을 파악하여 이를 토대로 모의 시나리오를 개발하였다. 다양한 밭경지 특성을 반영하기 위해 개발된 모의시나리오는 밭경지 규모 1 ha, 5 ha, 10 ha로 밭의 폭과 길이 비는 1 : 1를 적용하였으며, 밭의 경사는 7%, 15%를 고려하였다. 또한 강우조건은 50 mm, 100 mm, 150 mm, 200 mm 일강우량을 적용하였다. 그 결과, 초생대의 유출저감효과는 밭경사 7%와 15%에 대해 2.9~13.5%, 2.9~12.1%로 각각 나타났으며, 유사저감효과는 33.8~97.0%, 27.1~85.9%로 각각 나타나 유출저감효과에 비해 상대적으로 높은 저감효과를 나타내었다. 유출 및 유사 발생에 영향을 미치는 밭규모, 경사 및 강우량 인자에 따라 초생대 효과는 큰 차이를 나타내었다. 본 모의 결과를 바탕으로 새만금 유역내 밭경지 규모 10 ha 미만, 경사 15% 이내의 조건에서 밭면적 10% 규모로 초생대를 조성할 경우 유사저감 효과를 기대할 수 있을 것으로 사료된다. The study was intended to simulate the sediment reduction effects of the Vegetative Filter Strip (VFS) in uplands of Saemangeum watershed through VFSMOD-W model application. The model was calibrated by using the field data and the simulation scenarios were designed based on the investigation of uplands characteristics in Saemangeum watershed. The simulation scenarios were considered various size and slope of uplands including 1 ha, 5 ha, 10 ha of field size with width-length ratio of 1 : 1 having 7% and 15% of slopes under the daily rainfall of 50 mm, 100 mm, 150 mm, and 200 mm in order to mimic the different fields conditions. The effluent reduction ranged from 2.9~13.5% and 2.9~12.1% for runoff, and 33.8~97.0% and 27.1~85.9% for sediment under the field's slope of 7% and 15%, respectively. The VFS reduction effects showed different degree of influence from field size, slope, rainfall amounts. Based on the simulated results, the sediment contributing non-point source pollution expected to be reduced in the condition of VFS constructed 10% of fields in outlet of less than 10 ha of uplands having less than 15% of the slope.

Sentinel-2 위성영상을 이용한 DMZ 산불 피해 면적 관측 기법 연구

이슬기,송종성,이창욱,고보균,Lee, Seulki,Song, Jong-Sung,Lee, Chang-Wook,Ko, Bokyun 대한원격탐사학회 2022 大韓遠隔探査學會誌 Vol.38 No.5

본 연구는 직접적인 접근이 어려운 demilitarized zone (DMZ)의 산불 피해 지역을 파악하기 위하여, 고해상도 위성영상 및 머신러닝 기반의 감독 분류 기법을 이용하였다. 고해상도 위성 영상은 Sentinel-2 A/B를 이용하였으며, SVM 감독분류 기법을 기반으로 토지피복도를 산출하였다. DMZ 산불 피해 지역을 분류하기 위한 최적의 조합을 찾기 위하여 SVM 내에 다양한 커널과 밴드 조합에 따른 감독 분류를 진행하고 오차 행렬을 통해 정확도를 평가하였다. 또한, 2020년, 2021년은 위성영상 자료 기반의 산불 탐지 결과와 산불 연보의 피해 지역 면적 간의 비교를 통한 검증을 수행하였다. 이후, 현재 피해 면적 자료가 없는 2022년의 산불 피해 지역을 탐지함으로써 신뢰할 만한 수준의 결과를 신속적으로 파악하고자 하였다. This study used high-resolution satellite images and supervised classification technique based on machine learning method in order to detect the areas affected by wildfires in the demilitarized zone (DMZ) where direct access is difficult. Sentinel-2 A/B was used for high-resolution satellite images. Land cover map was calculated based on the SVM supervised classification technique. In order to find the optimal combination to classify the DMZ wildfire damage area, supervised classification according to various kernel and band combinations in the SVM was performed and the accuracy was evaluated through the error matrix. Verification was performed by comparing the results of the wildfire detection based on satellite image and data by the wildfire statistical annual report in 2020 and 2021. Also, wildfire damage areas was detected for which there is no current data in 2022. This is to quickly determine reliable results.

도시재생사업에서의 갈등사례 유형 분석

이슬기,권해림,유정호,Lee, Seul-Ki,Kwon, Hea-Rim,Yu, Jung-Ho 한국건설관리학회 2009 한국건설관리학회 논문집 Vol.10 No.6

대규모의 다수 프로젝트가 동시에 장기간에 걸쳐 이루어지는 도시재생사업은 넓은 수행범위와 사업의 다양성, 복합성에 기인한 불확실성을 가지고 있으며 다수의 관련주체들 간 이해관계의 복잡성으로 인해 사업 생애주기 동안 갈등의 발생은 필연적이고 그 사안의 성향 및 크기 또한 다양하다. 따라서 사전에 위험을 인지하고 적절하게 대응하는 적극적인 갈등관리가 절실히 필요하다. 따라서, 본 연구는 관련주체들이 사전에 발생 가능한 갈등을 인지하여 이에 대한 대비책 모색에 참조할 수 있도록 하며, 이러한 정보들을 사용자가 사용목적에 맞게 활용할 수 있도록 도시재생사업의 단계별, 주체별 갈등사례들을 유형화하고 주요 갈등유형을 제시 해보고자 한다. Because of a wide range of performance and diversity of project and uncertainty caused by complexity and a number of stakeholder, conflict occur necessarily in Urban generation including large-scale and many project which do the same time, for long time. so active conflict management which recognized risk before the fact and deal with conflict is necessarily needed. Therefore, This study is typing conflict cases and indicating critical conflict type through analysis on conflict case about process and stakeholder of urban generation to offer information which is referred to finding a solution when conflict occur by forecasting conflict that can occur before the fact.

익산 밭경지 특성을 고려한 초생대 유출 및 유사 저감효과 모의

이슬기,장정렬,최경숙,Lee, Seul Gi,Jang, Jeong Ryeol,Choi, Kyung Sook 한국농공학회 2014 한국농공학회논문집 Vol.56 No.5

The goal of this study was to evaluate sediment reduction effects of VFS (vegetative filter strip) applied for Iksan area in Saemangeum watershed. This study simulated runoff and sediment load from different types of uplands using VFSMOD-W. The general upland characteristics of the study area was investigated to build reasonable scenarios of the simulation. The simulation scenarios were designed by various areas, shapes, and slopes of uplands. Grass mixture was selected as VFS vegetation and the size of VFS was fixed as 10 % of uplands area. Additionally 50mm, 100mm, 150mm of daily rainfall were applied for the runoff and sediment simulation. As results, the calculated runoff and sediment loads were obtained $20.7{\sim}1,030.6m^3$ and 568.4~675,731.4 kg for the range of 0.1~1.0 ha of uplands with 7 % and 15 % slopes. The reduction effects on runoff and sediment were obtained 5~10 % and 21.0~47.7 % respectively from VFS applications. The VFSMOD-W simulations showed that runoff tended to increase as upland area and amount of rainfall increased while sediment increased when slope, length and area of uplands and amount of rainfall increased. These results indicated that rainfall amount and upland size are the critical factors for the generation of runoff and sediment load. In order to support this conclusion, further studies such as, long term monitoring, field experiments, and to calibrate and evaluate the model are necessary.

"황토를 이용한 건축재분야" 특허동향보고서

이슬기,Lee, Seul-Gi 한국특허정보원 2007 Patent 21 Vol.74 No.-

Chitosan-HNT 복합막을 이용한 기체 투과특성에 관한 연구

이슬기,홍세령,Lee, Seul Ki,Hong, Se Ryeong 한국막학회 2014 멤브레인 Vol.24 No.5

Chitosan에 HNT (halloysite nanotube)의 함량을 0, 3, 5, 10 wt%로 가하여 chitosan-HNT 복합막을 제조하였다. 복합막의 구조는 FT-IR, XRD, TGA, SEM으로 알아보았다. 기체투과 실험은 $30^{\circ}C$, $4kg/cm^2$ 조건에서 수행하였고, chitosan-HNT 복합막의 HNT 함량 변화에 따른 $CO_2$와 $CH_4$의 기체투과도와 선택도를 조사하였다. Chitosan-HNT 복합막의 기체 투과도는 HNT 함량이 3 wt%에서 가장 큰 값을 보였고, 그 이상의 함량에서는 감소하였다. 선택도($CO_2/N_2$)는 0~10 wt% 범위에서 HNT 내의 OH기와 $CO_2$ 간의 친화력으로 인하여 증가하였고, 약 1.3~3.8의 값을 보였다. Chitosan-HNT (halloysite nanotube) composite membranes were prepared by the addition of HNT 0, 3, 5, and 10 wt%. The structure of composite membranes were studied by FT-IR, XRD, TGA, and SEM. Gas permeation experiment were performed under condition of $30^{\circ}C$ and $4kgf/cm^2$. Gas permeability and selectivity were investigated by increasing the amount of HNT contents in the chitosan. Chitosan-HNT composite membrane for $CO_2$ and $CH_4$ showed the maximum value at 3 wt% of HNT content and decreased thereafter. The selectivity of ($CO_2/CH_4$) was increased due to its affinity with the OH groups on the HNT, was shown in the range of 1.3 to 3.8 at 0~10 wt%.