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모발에서 마약류 분석의 법과학적 고찰 - 마약류 범죄수사에서 모발 감정결과의 증거사용에 대해서 -
박용훈,한은영,이수연,Park, Yong-Hoon,Han, Eun-Young,Lee, Soo-Yeun 대한약학회 2008 약학회지 Vol.52 No.6
Hair analysis for drugs of abuse offers the crucial potential advantage when compared to urine, such as the longer time window of drug intake, which makes retrospective investigation of chronic and/or past consumption. This paper reviews the physiological basis of hair growth, mechanism of drug incorporation, analytical methods, result interpretation and practical application of hair analysis. Moreover, to facilitate the court's decision regarding specific circumstances surrounding the crime, this review demonstrated that the results of hair analysis not only should be admitted as scientific evidence of drug use but also could legally improve reliability of the evidence.
HPLC 형광검출법에 의한 Glyphosate의 혈중농도 측정
이상기(Sang Ki Lee),김기욱(Ki Wook Kim),양자열(Ja Youl Yang),인상환(Sang Whan In),이수연(Soo Yeun Lee) 大韓藥學會 2001 약학회지 Vol.45 No.4
A rapid and sensitive method for the determination of glyphosate, a phosphates amino acid herbicide, in whole blood is presented. After removal of protein, the whole blood was purified by using the anion exchange resin (Dowex 1), and derivatized with 9- fluorenylmethyl chloroformate (FMCL). Derivatized glyphosate from blood sample was injected onto a Whatman partisil 10SAX column and separated with 0.1M phosphate buffer (pH 2.5) and acetonitrile (ratio=3:1). The high performance liquid chromatography- fluorescence detection gave the detection limit of 86pg and linearity of 0.9999 in the range of 0.25μg/ml and 25μg/ml. The recoveries of glyphosate added to the blood samples were ranged from 75.3% to 100.4% compared to the samples prepared in water. The derivatized glyphosate was stable at various acidity and temperature. This method has been successfully applied to the blood samples of lethal intoxication with the herbicide glyphosate.
김지숙(Ji-sook Kim),이수연(Soo-yeun Lee),임종인(Jong-in Lim) 한국정보보호학회 2010 정보보호학회논문지 Vol.20 No.2
정보보호 관리체계 구축을 지원하기 위해 민간기업 대상으로는 "정보통신망 사용 촉진 및 정보보호 등에 관한 법률"에 근거하여 한국인터넷진흥원이 인증하는 ISMS제도가 있으며 공공기관 대상으로는 아직 인증제도는 없으나 "전자 정부법"에 근거하여 국가정보원이 '정보보안 관리실태 평가제도를 운영하고 있다. 본 논문에서는 민간부문과 공공부문에서 시행하고 있는 정보보호 관리체계 통제항목에 대한 비교와 함께 그간 실시한 평가 미흡사항을 분석하여 이를 토대로 효율적인 정보보호 관리체계 구축방안을 살펴보고자 한다. To support the establishment of Information Security Management System, the private sector and the public sector have taken some measures. In the private sector, KISA(Korea Internet & Security Agency) has certified ISMS system based all "The Act on Communication Network Use Promotion and Information Security etc.". In the public sector, No authentication system has been established. Instead, NIS(National Intelligence Service) has enforced 'Infonrmation Security Management Condition Evaluation' based on "Electronic Government Act". This article compared ISMS control parts of the private sector with that of the public sector and analyzed the non-enforcemem parts of ISMS implementing two sectors for years. Based 011 this, I would like to consider the method of establishment for efficient ISMS.
유영찬(Young Chan Yoo),이상기(Sang Ki Lee),이수연(Soo Yeun Lee),양자열(Ja Yeol Yang),인상환(Sang Whan In),김기욱(Ki Wook Kim),정규혁(Kyu Hyuck Chung) 대한약학회 2001 약학회지 Vol.45 No.4
Persistent organochlorine pesticides has been used intensively in agriculture for a long time. The occurrence of organochlorine pesticides in the environment and subsequently in parts of the food chains, results in the intake of these compounds by human and animal. The measurement of the levels of organochlorine pesticides in tissues or blood of human populations is a good marker in evaluating the extent of exposure and hazards. So, most countries have conducted initial monitoring programs to determine organochlorine pesticides and polychlorinated biphenyls in human tissues. But, in Korea, a few research has been reported. In this study the legally prohibited organochlorines, such as, α-BHC, β-BHC, γ-BHC, δ-BHC, p,p'-DDT p,p'-DDD, p,p'-ODE, endrin, dieldrin and aldrin were determined in human blood, brain, adipose tissue, kidney cortex and liver tissues collected at autopsy of 10 men and 10 women, 16-65 years of age. Almost all the organochlorine pesticides mentioned previously were determined in human tissue samples indicated that they have been widely distributed in Korean human body: No significant difference was found in the levels of organochlorine pesticides between sexes, districts and ages.
한국인 지방,간장 및 혈액 웅 유기염오제류 및 PCB congeners의 분포
유영찬(Young Chan Yoo),이상기(Sang Ki Lee),김기욱(Ki Wook Kim),이수연(Soo Yeun Lee),양자열(Ja Youl Yang),김윤신(Youn Shin Kim),오승민(Seung Min Oh),정규혁(Kyu Hyuck Chung) 대한약학회 2002 약학회지 Vol.46 No.4
Persistent organochlorine pesticides and polychlorinated biphenyls (PCBs) have been used intensively in agriculture or industry for a long time. The occurrence of organochlorine pesticides and PCBs in the environment and subsequently in parts of the food chain, resulting in the intake of these compounds by man and animal. The measure of the levels of organochlorine pesticides and PCBs in tissues or blood of human populations are good markers in determining the extent of exposure and in the evaluating the hazards. So, most countriess have conducted initial monitoring programs to determine organcchlorine pesticides and PCBs in human tissues. But a few report has been presented in Korea. In this study α-BHC, β-BHC,γ-BHC, δ-BHC, p,p'-DDT p,p'- DDD, p,p'-DDE, endrin, dieldein, aldrin and 7 marker PCBs (28,52, 101, 118, 138, 153, 180) were determined in human blood, adipose tissue and liver tissues collected at autopsy of 10 men and 10 women, 13-79 year of age. Significant differences in the levels of organochlorine pesticides and PCBs between districts where they had lived were found in the following chemicals : total PCB in the blood : β-BHC, total BHC, p,p'-DDE and total DDT in the adipose tissue : p,p'-DDE, total DDT and PCB 118 in the liver. No significant difference was fecund in the levels of organochlorine pesticides and PCBs between sexes and ages. Though the levels of organochlorine pesticides and PCBs were relatively lower than that of other countries, we could know that organochlorine pesticides and PCBs have been widely distributed in Korean human body.
진혜진(Hye Jin Jin),최수진(Soo Jin Choi),배윤경(Yun Kyung Bae),이수연(Soo Yeun Lee),길명옥(Myeong Og Gil),박찬미(Chan Mi Park),왕준호(Joon Ho Wang),오원일(Wonil Oh),양윤선(Yoon Sun Yang),조인호(Inho Jo),김철기(Cheol-Ki Kim),송해룡(Hae-R 대한정형외과학회 2007 대한정형외과학회지 Vol.42 No.6
목적: 인간 골수로부터 간엽줄기세포를 분리 배양하여 기증자의 나이에 따른 분화능력을 비교하였다. 대상 및 방법: 저연령 그룹 (n=16, 12.5±5.8세)와 고연령 그룹 (n=4, 48.5±7.2세)으로 나눠 총 20명의 환자로부터 동의를 얻어 골수를 채취하였고, 채취한 골수로부터 간엽줄기세포를 추출하였으며, 세포 형태와 면역표현형을 분석하였다. 또한, 세포의 노화를 두 그룹에서 계대 배양하여 측정하였다. 골, 연골, 지방세포로의 분화를 관찰하기 위해 역전사 중합효소연쇄반응을 수행하였으며, alkaline phosphatase, von Kossa, safranin O와 oil red O 염색을 시행하였다. 결과: 총 20명의 골수에서 모두 간엽줄기세포를 추출하였고, 저연령 그룹에서 획득한 간엽줄기세포의 수가 고연령 그룹에 비해 약 5배 많았다. 세포의 노화는 두 그룹 모두 10세대 이후에서 나타났으며, 면역표현형도 두 그룹에서 유사한 형태를 보였다. 골, 연골, 지방세포로의 다양한 분화능력을 보였으며, 두 그룹간의 차이는 없었다. 결론: 본 연구의 결과는 골수 기증자의 나이는 골수 간엽줄기세포의 분화능력에 영향을 주지 않음을 보여 주었다. Purpose: The aim of study was to compare the differentiation capacity of mesenchymal stem cells (MSCs) obtained from human bone marrow (BM) according to the age of the donors. Materials and Methods: MSCs were isolated from the BM of young (n=16, 12.5±5.8 years) and elder (n=4, 48.5±7.2 years) patients with the consent of them. We analyzed the cell morphology and the cell surface markers of the MSCs. In addition, we assessed the cell senescence with serial cultures from both age groups. Cell pluripotentiality was analyzed by osteogenic, chondrogenic, and adipogenic induction media. We performed RT-PCR, a measurement of expression of alkaline phosphatase, and staining with von Kossa, safranin O, and oil red O stain. Results: All of the MSC samples tested, irrespective of the age of the donors, MSCs were all successfully isolated from twenty bone marrows. However, the number of cells of from the young donors was five times greater than that of the elderly donors. Senescence was observed over 10 passages in both age groups. The immunophenotypes of both age groups showed similar patterns. MSCs obtained from young and older donors showed the potential to differentiate into osteogenic, chondrogenic, and adipogenic lineages with no difference for both age groups. Conclusion: Our study supports that age does not influence the pluripotential capacity of human BM derived MSCs.