CO₂ 농도 및 기온 상승에 대한 현사시나무의 광합성 반응

이솔지,오창영,한심희,김기우,김판기,Lee, Solji,Oh, Chang-Young,Han, Sim-Hee,Kim, Ki Woo,Kim, Pan-Gi 한국농림기상학회 2014 한국농림기상학회지 Vol.16 No.1

지구온난화와 같은 기후변화에 적응력이 높은 조림수종을 탐색하는 연구의 일환으로 $CO_2$농도 및 기온상승이 현사시나무의 광합성생리에 미치는 영향을 조사하였다. 그 결과 현사시나무는 $CO_2$농도 및 기온 상승에 의해서 줄기의 신장생장이 억제되고 광합성 능력이 저하되었다. 그리고 광합성능력과 관련된 색소(엽록소a, b, 카로티노이드)의 함량이 감소하였다. 특히 탄소고정계의 활성과 관련된 엽록소a의 감소가 현저하게 나타났다. 그리고 광-광합성곡선과 A-Ci곡선에서 광화학계의 활성을 나타내는 순양자수율이 7%, 전자전달속도가 14% 감소하고, 탄소고정계의 활성을 나타내는 탄소고정효율이 52%, 재인산화속도가 24% 감소하였다. 이러한 결과로 $CO_2$농도 및 기온 상승에 의한 현사시나무의 광합성능력 저하는 광화학계 및 탄소고정계의 활성저하에 기인하나, 탄소고정계의 활성저하가 더 크게 작용하였음을 알 수 있다. This study was conducted to investigate the photosynthetic characters of Populus alba${\times}$glandulosa cuttings in response to elevated $CO_2$ concentration and air temperature for selecting tree species adaptive to climate change. The cuttings were grown in environment controlled growth chambers with two combinations of $CO_2$ concentration and air temperature conditions: (i) $22^{\circ}C$ + $CO_2$ 380 ${\mu}mol$ $mol^{-1}$ (control) and (ii) $27^{\circ}C$ + $CO_2$ 770 ${\mu}mol$ $mol^{-1}$ (elevated) for almost three months. The cuttings under the elevated treatment showed reduced tree height and photosynthetic pigment contents such as chlorophyll and carotenoid. In particular, the elevated treatment resulted in a marked reduction in the chlorophyll a closely associated with $CO_2$ fixative reaction system. Different levels of reduction in photosynthetic characters were found under the elevated treatment. A decrease was noted in photochemical reaction system parameters: net apparent quantum yield (7%) and photosynthetic electron transport rate (14%). Moreover, a significant reduction was obvious in $CO_2$ fixative reaction system parameters: carboxylation efficiency (52%) and ribulose-1,5-bisphosphate(RuBP) regeneration rate (24%). These results suggest that the low level of photosynthetic capacity may be attributed to the decreased $CO_2$ fixative reaction system rather than photochemical reaction system.

창의적 공간설계를 위한 Geodesign Interface 연구

이솔지,김은형,Lee, Sol-Ji,Kim, Eun-Hyung 한국국토정보공사 공간정보연구원 2016 지적과 국토정보 Vol.46 No.2

Dangermond(2009)에 의해 GIS의 다음 패러다임으로서 제시된 Geodesign은 공간계획 및 설계의 바탕이 된다. 또한 고재용(2015)은 공간정보를 기초로 하여 진행되는 조경설계의 관점에서 Geodesign 방법론을 제시하였다. 그러나 그의 방법론은 효율성 향상을 위한 연구인 반면 Geodesign의 한계점이자 공간설계의 핵심인 '창의성 지원'은 다루지 못하였다. 이러한 문제점을 보완하기 위해서는 공간설계의 관점에서 전 과정에 대한 정보를 효과적으로 다룰 수 있도록 지원하는 개념으로써 개념화를 통해 $7{\pm}2$ 개라는 인간의 인지능력의 한계를 극복해야 한다. 따라서 본 논문에서는 그러한 한계점을 극복하고자 조경설계 분야의 사용자에게 더 나은 경험을 제공하기 위해 인간의 인지능력한계 극복과 그것을 통한 창의성 향상을 목표로 연구를 진행하였다. 선행연구의 고찰을 통해 창의성 향상을 위한 방안을 도출하였으며, 이 방안을 바탕으로 창의적 공간설계의 지원이 가능한 Geodesign 방법론을 제시하였다. 제시된 방법론의 과정을 수행하기에 가장 적합하다고 판단된 ESRI사의 GeoPlanner for ArcGIS에 방법론을 적용하여 Interface 설계의 과정을 수행하였다. 먼저 스크립트를 작성하고 기능요소와 정보요소를 판단하여 Interaction을 도출하였고, 도출된 Interaction을 바탕으로 Interface 설계를 진행하였다. 창의성 향상이라는 것은 물리적으로 증명하기는 어려운 부분이다. 그러나 Interface 설계를 통해 잠재의식 속에 있던 새로운 질서를 찾아내어 개념화를 통한 창의적 지원이 가능하며, 순간적으로 떠오르는 아이디어를 놓치지 않고 표현하여 인지능력의 한계를 극복할 수 있는 환경을 제안하였다. Geodesign was suggested by Dangermond(2009) as a next paradigm of GIS to be a future basis for spatial planning and design. Ko(2015) also suggested a geodesign methodology that uses spatial information from a landscape designer's perspective. Spatial planning and design fundamentally require creativity and efficiency but Ko's methodology did not include the creativity aspect. To complement the deficiency, this paper expand the research scope to deal with a designer's cognitive limits and to provide better experiences for the landscape designers. An interface was designed to improve the designer's creativity based on interactions that were derived from a script of landscape design. The expanded methodology for both efficiency and creativity is suggested for the interface by analyzing preceding researches. ESRI's GeoPlanner for ArcGIS, as a first geodesign software, is compared to improve the interface in terms of creativity. One of the difficulties as a thesis is that it is not easy to measure the improvement of design creativity physically and quantitatively. This paper tried to eliminate any stumbling block in supporting creativity and to help designers find new orders in design subconsciousness and to reach a new concept. In addition, the interface to express instantaneous design ideas would be a guide to overcome the designer's cognitive limits.

유칼립투스 추출물의 Propionibacterium acnes에 의해 유도되는 염증반응 억제 효과

이솔지,이은혜,신진학,김선숙,김남경,최은미,서수련,Lee, Sol Ji,Lee, Eun Hye,Shin, Jin Hak,Kim, Seon Sook,Kim, Nam Kyoung,Choi, Eunmi,Seo, Su Ryeon 한국미생물학회 2016 미생물학회지 Vol.52 No.4

여드름은 만성 염증 질환으로 주로 청소년기에 발생하는 것으로 알려져 있으나 대기오염, 약물 남용 등의 원인에 의해 아동기 및 성인기에도 나타날 수 있다고 알려져 있다. 여드름을 유발하는 정확한 원인은 밝혀져 있지 않으나 한 가지 원인보다 스트레스, 호르몬의 변화, 유전, 외부 환경 등 다양한 요인들이 복합적으로 작용한다고 여겨지고 있다. Propionibacterium acnes (P. acnes)는 여드름을 유발하는 균으로 모낭 내에 상주하여 피지의 중성지방을 분해하고 유리 지방산을 형성하여 모낭 내 염증을 유발한다. 따라서 피지의 생성 증가는 P. acnes의 생존에 좋은 영향을 주고 피부에서 염증반응을 유발하는 monocytic cell의 활성화와 염증성 사이토카인(pro-inflammatory cytokine)의 증가를 유발한다. 따라서 여드름 치료를 위해서는 P. acnes의 증식 억제 및 염증반응을 최소화하는 것이 중요하다. 본 논문에서는 유칼립투스(Eucalyptus globules) 추출물이 P. acnes에 의한 염증반응에 나타내는 효과를 확인하고자 하였다. 유칼립투스 추출물 처리는 P. acnes가 유도하는 염증 매개자로 알려진 $TNF-{\alpha}$, $IL-1{\beta}$, IL-2와 인플라마좀 복합체인 NLRP3의 유전자 발현을 효과적으로 감소시키는 것을 확인하였다. 뿐만 아니라 염증성 사이토카인의 유전자 발현에 중요하다고 알려진 전사인자(transcription factors) $NF-{\kappa}B$와 NFAT의 활성 역시 유칼립투스 추출물을 처리하였을 때 감소하는 것을 알 수 있었다. 따라서 본 논문을 통해 유칼립투스 추출물이 P. acnes에 의해 초래되는 여드름의 치료 보조제로 사용될 수 있으며, 천연 추출물의 사용이 항생제 장기 복용으로 인해 유발되는 항생제 내성을 해결하는 좋은 대안이 될 것이라고 예상할 수 있다. Acne is known as the most common skin disease. It commonly occurs during adolescents, but it is also present in children and adults because of air pollution, drug abuse and so on. In addition to the hormonal, genetic and environmental factors, Propionibacterium acnes (P. acnes) have also critical roles in outbreak of acne by inducing inflammatory mediators. Increase of sebum production provides an ideal environment for P. acnes that induce inflammation on the skin by activation of monocytic cells and stimulation of inflammatory cytokines. In this study, natural extracts were investigated for anti-inflammatory effects against inflammatory acne by P. acnes infection in terms of reducing cytokine production. Eucalyptus globulus extracts effectively suppressed mRNA synthesis of inflammatory mediators such as $TNF-{\alpha}$, $IL-1{\beta}$, IL-2, and NLRP3 in P. acnes-activated macrophages. Moreover, Eucalyptus globulus extracts inhibit activation of transcription factors, $NF-{\kappa}B$ and NFAT, which are known as key regulators of inflammatory cytokine production. This study suggests the potential of using Eucalyptus globulus extracts as alternative agents for the treatment of acne.

정신건강사회복지 전문가의 비대면 정신건강 모바일 서비스 플랫폼 개발 및 상담 경험에 관한 자문화기술지

이솔지,Lee, Sol-Ji 한국사물인터넷학회 2022 한국사물인터넷학회 논문지 Vol.8 No.5

This study is a study that collects and analyzes data through An autoethnographyon as one of method of qualitative research, through which researcher have experienced firsthand in the process of developmenting and counseling there a new concept professional mental health mobile service platform as a mental health social welfare expert. The results of this study are as follows.First, there are people in Korea who are directly exposed to mental health issues and need to receive professional medical services, but there are also quite a few members of the public who have a desire for general mental health services, although the level of therapeutic intervention is not required. Second, considering the characteristics of modern society and especially in the pandemic situation of infectious diseases such as COVID-19, more and more people are preferring various mental health service platforms using mobile devices. Third, all generations complain of mental health issues such as family problems, stress, interpersonal relationships, and occupations, and other mental health problems such as self-harm and suicide accidents are frequently mentioned in connection with mental disorders such as depression, anxiety disorder, addiction, and post-traumatic stress disorder. Fourth, youngth s were exposed to various mental health-related issues, and above all, they were complaining of serious mental health problems such as self-harm and suicide accidents. Fifth, in delivering professional mental health services, mental health experts with the ability also to provide professional services online through mobile devices are needed as well offline. Finally, based on the results of this study, policy and practical suggestions related to the provision of mental health services in the future, and directions for follow-up studies were suggested.

꽈배기모자반의 발효를 통한 항염증 활성의 증진

이솔지(Sol-Ji Lee),이동근(Dong-Geun Lee),김미향(Mihyang Kim),공창숙(Chang-Suk Kong),유기환(Ki-Hwan Yu),김육용(Yuck-Young Kim),이상현(Sang-Hyeon Lee) 한국생명과학회 2016 생명과학회지 Vol.26 No.3

본 연구에서는 유산균으로 발효한 꽈배기모자반의 항염증 및 iNOS 발현저해 활성에 대하여 검증하였다. 항염증 활성은 lipopolysaccharide (LPS)로 자극시킨 RAW 264.7 세포에서 nitric oxide (NO)가 생성되는 양을 비교하여 나타내었으며, iNOS 발현저해 활성은 stable transfection시킨 RAW 264.7 세포에서 LPS에 의해 유도되는 inducible nitric oxide synthase (iNOS) 발현에 대한 저해효과를 reporter인 luciferase의 활성을 확인하여 나타내었다. NO 생성 억제능과 iNOS 발현에 대한 실험은 NO radical 소거 활성을 확인한 후 수행하였다. NO radical 소거 활성은 발효군이 비발효군에 비해 7.6~15.2% 증가되었으며, Lactobacillus sp. SH-1으로 접종한 군이 가장 높은 활성을 나타내었다. 그리고 해조류 침전물을 포함하여 발효한 군보다는 해조류 침전물을 포함하지 않고 발효한 군이 더 높은 NO radical 소거 활성을 나타내었다. Lactobacillus sp. SH-1을 접종하여 발효한 군은 LPS로 자극시킨 RAW 264.7 세포에서 NO 생성 억제능이 가장 높게 나타났다(64.1%). 또한 Lactobacillus sp. SH-1 접종군은 50, 100, 500, 1,000 ㎍/ml의 농도에서 LPS에 의해 유도된 iNOS 발현을 각각 28.6, 35.6, 49.4, 58.5% 감소시켰다. MTT법에 따르면, 발효 꽈배기모자반은 모든 농도에서 세포 생존율에 영향을 미치지 않았으므로, 세포독성을 나타내지 않는 것으로 사료된다. 본 연구에서는 NO radical 소거 활성, 항염증 및 iNOS 발현저해 활성 등의 꽈배기 모자반이 가지는 생리활성을 확인하였다. 따라서 발효에 의해 생리활성이 개선된 꽈배기모자반을 이용하여 기능성 식품을 개발할 수 있을 것으로 기대된다. This study was aimed to verify anti-inflammatory activity of fermented Sargassum siliquanstrum with lactic acid bacteria. Anti-inflammatory activities were compared by measuring the amount of nitric oxide (NO) in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and suppressive effect on inducible nitric oxide synthase (iNOS) expression in stably transfected RAW 264.7 cells. Inhibitory activities of NO production and iNOS expression were measured after confirmation of NO radical scavenging activities. Fermentation increased NO radical scavenging activities from 7.6% to 15.2% compared to non-fermented condition, and fermentation with Lactobacillus sp. SH-1 was the most efficient. Fermentation without algal debris showed better NO radical scavenging activities than that with debris. Fermentation with Lactobacillus sp. SH-1 also showed the highest NO production inhibitory activity (64.1%) in LPS-stimulated RAW 264.7 cells. LPS-induced iNOS expression was diminished to 28.6, 35.6, 49.4 and 58.5 at 50, 100, 500 and 1,000 μg/ml, respectively, by fermentation with Lactobacillus sp. SH-1. According to MTT assay, fermented S. siliquanstrum did not influence the cell viability at all concentrations tested, meaning no or less cytotoxicity. These results suggest that S. siliquanstrum has NO radical scavenging activity and anti-inflammatory activity. Thus biological activities of S. siliquanstrum were upgraded by fermentation, which could be used for the development of functional foods.

연구논문 : 학동기 아동용 닭찜의 관능적 기호도에 영향을 주는 요인 분석

이솔지 ( Sol Ji Lee ),류보경 ( Bo Kyung Ryu ),이지선 ( Ji Sun Lee ),이민아 ( Min A Lee ),홍상필 ( Sang Pil Hong ),정라나 ( La Na Chung ) 한국식생활문화학회 2015 韓國食生活文化學會誌 Vol.30 No.5

The purpose of this study was to analyze factors affecting acceptance of four kinds of dak-jjim samples in third year schoolaged children (n=100). Soy sauce, red pepper paste, Vietnam fish sauce, and star anise were applied to samples for their familiar and exotic characteristics. Significant differences among samples were observed in odor, taste, and acceptance (p<0.001). Soy (Soy sauce sample), RPPaste (Red pepper paste sample), and Soy_FishS (Soy sauce and Vietnam fish sauce sample) samples scored higher than Soy_StarA (Soy sauce and star anise sample) sample. On the other hand, there were no significant differences in intensities of saltiness, sweetness, and hot spicy flavor (p>0.05). Liked attributes in samples were saltiness, sweetness, chicken flavor, potato flavor, moistness of chicken, hot spicy flavor, and color. Disliked attributes in samples were hot spicy flavor, saltiness, and ginger flavor. Significant differences among samples were observed in familiar intensity and willing to try again (p<0.001). Soy, RPPaste, and Soy_FishS samples scored higher than Soy_StarA sample. Panels considered taste (46%) and nutrition (45%). Higher familiar intensity of sample was associated with higher acceptance in samples. In other words, familiarity of food affects acceptance of food. Therefore, familiar ingredients such as soy sauce and chicken can be used for development of Korean menu items considering taste and nutrition with enhanced Korean food acceptance in school-aged children.

Alteromonas sp. SH-1균 유래의 α-agarase의 특성조사

이솔지(Sol-Ji Lee),신다영(Da-Young Shin),김재덕(Jae-Deog Kim),이동근(Dong-Geun Lee),이상현(Sang-Hyeon Lee) 한국생물공학회 2016 KSBB Journal Vol.31 No.2

A novel agar-degrading marine bacterium, SH-1 strain, was isolated from seashore of Namhae at Gyeongnam province, Korea. The SH-1 strain exhibited 98% similarity with Alteromonas species based on 16S rDNA sequencing and named as Alteromonas sp. SH-1. Alteromonas sp. SH-1 showed agarase activity of 348.3 U/L (1.67 U/mg protein). The molecular masses of the enzymes were predicted as about 85 kDa and 110 kDa by SDS-PAGE and zymogram. The enzymatic activity was optimal at 30℃ and the relative agarase activity was decreased as temperature increase from 30℃ and thus about 90% and 70% activities were shown at 40oC and 50℃, respectively. The optimum pH was 6.0 for agarase activity in 20 mM Tris-HCl buffer and activities were less than 70% and 85% activity at pH 5.0 and pH 7.0, respectively, compared with that at pH 6. Agarase activity has remained over 90% at 20℃ after 1.5 hour exposure at this temperature. However, its activity was less than 60% at 30℃ after 0.5 h exposure at this temperature. The enzymes produced agarooligosaccharides such as agaropentaose and agarotriose from agarose, indicating that the agarases are α-agarases. Thus, Alteromonas sp. SH-1 and its agarases would be useful for the industrial production of agarooligosaccharides which are known as having anticancer and antioxidation activities.

해양성 Simiduia sp. SH-1 균주의 분리 및 한천분해효소의 특성조사

이솔지(Sol-Ji Lee),오수정(Soo-Jeong Oh),이동근(Dong-Geun Lee),이상현(Sang-Hyeon Lee) 한국생명과학회 2015 생명과학회지 Vol.25 No.11

경상남도 남해군 연안의 해수를 이용하여 신규의 한천분해 해양성세균을 분리하고 한천분해효소의 특징을 분석하였다. Marine agar 2216 배지에서 분리한 SH-1 균주는 16S rRNA 유전자 염기서열분석을 통해 Simiduia 속 세균과 99% 유사하여 Simiduia sp. SH-1으로 명명하였다. Simiduia sp. SH-1 균주가 생성하는 한천분해효소는 성장의존성 산물로 판단되었으며 정체기부터 효소활성이 감소되었다. 한천분해효소는 pH 7.0(20 mM Tris-HCl buffer), 30℃에서 최대활성(698.6 units/l)을 나타내었다. 효소의 활성은 30℃에서 최적이었고 이후 온도가 증가함에 따라 활성이 감소하였으며, 40℃와 50℃에서 각각 약 90%와 75%의 상대활성을 보였으나 내열성은 보이지 않았다. 최적 pH인 pH 7.0에 비해 pH 6.0에서는 오차범위 내에서 조금 낮은 활성을 보였으며 pH 5.0과 pH 8.0에서는 각각 80%와 75% 정도의 상대활성을 보였다. TLC 분석을 통하여 Simiduia sp. SH-1 균주가 생성하는 한천분해효소는 agarose를 분해하여 피부의 미백활성, 전분노화의 방지 및 세균성장의 억제 등의 유용한 효과를 가지는 기능성 한천올리고당인 neoagarotetraose와 neoagarobiose를 최종적으로 생성하는 β-agarase로 확인되었다. 따라서, Simiduia sp. SH-1 균주와 이 균주가 생산하는 β-agarase는 산업적 생산에 유용하게 활용될 수 있을 것으로 기대된다. Agarase from a novel agar-degrading bacterium isolated from seawater in Namhae at Gyeongsangnamdo province of Korea was characterized. The SH-1 strain was selected from thousands of colonies on Marine agar 2216 media. Almost full 16S rRNA gene sequence of the agarolytic SH-1 strain showed 99% similarity with that of bacteria of Simiduia genus and named as Simiduia sp. SH-1. Agarase production was growth related, and activity was declined from stationary phase. Secreted agarase was prepared from culture media and characterized. It showed maximum activity of 698.6 units/L at pH 7.0 and 30°C in 20 mM Tris-HCl buffer. Agarase activity decreased as the temperature increased from an optimum of 30° C, with 90% and 75% activity at 40°C and 50°C, respectively. Agarase was not heat resistant. Slightly lower agarase activity was observed at pH 6.0 than at pH 7.0, without statistical difference, and 80% and 75% activity were observed at pH 5.0 and 8.0, respectively. Neoagarotetraose and neoagarobiose were the main final products of agarose, indicating that it is β-agarase. Simiduia sp. SH-1 and its β-agarase would be useful for the industrial production of neoagarotetraose and neoagarobiose, which have a whitening effect on skin, delaying starch degradation, and inhibiting bacterial growth.

신규 한천분해세균 Maribacter sp. SH-1의 분리 및 효소 특성조사

이창은 ( Chang Eun Lee ),이솔지 ( Sol Ji Lee ),이동근 ( Dong Geun Lee ),이상현 ( Sang Hyeon Lee ) 한국미생물생명공학회(구 한국산업미생물학회) 2016 한국미생물·생명공학회지 Vol.44 No.2

본 연구에서는 신규 해양성 한천분해균을 분리하고, 이 균주가 생성하는 한천분해효소의 특성을 조사하였다. 경상남도 남해군 미조면 연안에서 채취한 해수를 Marine agar 2216 배지에 도말하여 한천분해세균을 선별하였다. 선택된 한천분해균주는 16S rDNA 염기서열분석을 통해 Maribacter속 세균과 99% 유사하여 Maribacter sp. SH-1으로 명명하였다. 세포외로 분비되는 agarase는 Maribacter sp. SH-1 균주 배양액에서 획득하였으며, 이를 이용하여 특성을 조사 하였다. Maribacter sp. SH-1 균주의 한천분해효소는 20, 30, 40, 50 및 60℃에서 각각 56, 62, 94, 100, 8%의 상대활성을 나타냈으며, pH 5, 6, 7 및 8에서 각각 15, 100, 60, 21%의 상대활성을 나타냈다. 세포외 agarase는 50℃, pH 6인 20 mM Tris-HCl buffer를 사용하는 조건에서 최대활성(231 units/l)을 보였다. 한천이 sol 상태로 존재하는 50℃에서 최적활성을 보여 이 효소는 응용 가능성이 높다고 할 것이다. 효소 활성은 20, 30 및 40℃에서 30분 동안 열처리하였을 때 약 90% 이상의 상대활성을 보였다. TLC 분석 결과, Maribacter sp. SH-1 균주의 한천분해효소는 한천올리고당인 neoagarohexaose (34.8%), neoagarotetraose (52.2%) 및 neoagarobiose (13.0%)를 생성하는 것으로 보아 β-agarase로 확인되었다. 따라서 Maribacter sp. SH-1 균주와 이 균주가 생산하는 β-agarase는 보습효과, 미백효과, 세균성장 억제 혹은 전분노화 방지 등의 기능을 가지는 한천올리고당의 생산에 유용할 것으로 기대된다. In this study, we isolated a new agar-degrading marine bacterium and characterized its agarase. An agardegrading marine bacterium SH-1 was isolated from seawater, collected from the seashore of Namhae in Gyeongnam province, Korea, and cultured in marine agar 2216 media. It was identified as Maribacter. sp. SH-1 by phylogenetic analyses, based on 16S rRNA gene sequence. The extracellular agarase was extracted from culture media of Maribacter sp. SH-1 and characterized. Its relative activities were 56, 62, 94, 100, and 8% at 20, 30, 40, 50, and 60°C, respectively, whereas 15, 100, 60, and 21% relative activities were observed at pH 5, 6, 7, and 8, respectively. Its extracellular agarase exhibited maximum activity (231 units/l) at pH 6.0 and 50°C, in 20 mM Tris-HCl buffer. Therefore, this agarase would be applicable as it showed the maximum activity at the temperature at which the agar is in a sol state. Furthermore, the agarase activities remained over 90% at 20, 30, and 40°C after 0.5 h exposure at these temperatures. Thin layer chromatography analysis suggested that Maribacter sp. SH-1 produces extracellular β-agarase, as it hydrolyzes agarose to produce neoagarooligosaccharides, such as neoagarohexaose (34.8%), neoagarotetraose (52.2%), and neoagarobiose (13.0%). Maribacter sp. SH-1 and its β-agarase would be useful for the production of neoagarooligosaccharides, which shows functional properties, like skin moisturizing, skin whitening, inhibition of bacterial growth, and delay in starch degradation.

1인가구 주거공간을 위한 크리에이티브 디자인방법 연구

이솔지(Lee, Sol-Ji),김지은(Kim, Ji-Eun) 한국실내디자인학회 2018 한국실내디자인학회논문집 Vol.27 No.5

Recently, the number of single household increased rapidly, causing multiple changes in society and culture. The lifestyle and values of single households have subdivided into various types, which demand new forms of residential space. There were several research about living space of single household, but the number of design research about living space that focuses on life of single household lacks severely. For this reason, this research focuses on proposing creative design method that will be used as a specific basis of designing residential space which provides prosperous single household. This research will check lifestyle of single household and derived seven needs of residential space of single household by examining collision with current living space. Also, this research deduced nine characteristics of reformative house by analyzing Japan"s house exhibition which has multiple revolutionary ideas about residence. By merging the seven needs and the nine characteristics, the researcher founds five types of design method. The design method derived from this research will be fundamental material for design of future housing. Moreover, research on residential space using formative language based on the derived design method will be helpful to qualitative research on single household. Therefore, the researcher will conduct succeeding study about residential design of single household by putting this theory in practice.