복령 균핵의 에탄올 추출물에서 분리한 화합물의 조골세포 분화 촉진 및 파골세포 생성 억제 효과

이소라,김석주,문보욱,최식원,유림,이형원 한국산림과학회 2024 한국산림과학회지 Vol.113 No.1

복령(Wolfiporia hoelen (Fr.) Y.C.Dai & V. Papp)은 아시아 및 유럽 등 많은 나라에서 약용 및 식용으로 사용하고있는 중요한 약재이다. 복령의 주요 성분들의 약리 활성이 많은 연구를 통해 밝혀지고 있으며, 골대사에도 영향을 주는 것으로 나타났다. 이에 본 연구는 복령 균핵 50% 에탄올 추출물이 골질환 예방 및 천연 치료제의 소재로 활용 가능성이 있는지 알아보고자 하였다. 복령 균핵의 에탄올 추출물을 계통분획법에 따라 n-헥산, 디클로로메탄, 에틸아세테이트순으로 분획하여 C2C12세포에 처리하였으며, 디클로로메탄 분획물에서 C2C12 세포의 ALP 분화 활성이 대조구보다 약 29%가증가함을 알 수 있었다. 활성을 보인 디클로로메탄 분획물로부터 총 4개의 화합물을 분리하였으며, 기기분석 결과와 문헌을 참고하여 3α-dehydrotrametenolic acid, ergosterol, pachymic acid와 dehydrotumulosic acid로 동정할 수 있었다. 골대사 효능이 있는 지표물질을 찾기 위하여 4개의 화합물을 C2C12 세포의 ALP 분화 활성과 BMMs의 RANKL 유도 억제 활성을농도별(1, 3, 10, 30, 100 μM)로 평가하였으며, 세포독성 검정도 동일한 농도에서 수행하였다. 그 결과 분리된 화합물 중dehydrotumulosic acid의 3 μM 농도에서 ALP 활성이 160%로 나타났으며, BMP-2 대조구보다 약 24% 유의미하게 증가하였다. 또한 BMMs의 RANKL 유도 억제 활성은 dehydrotumulosic acid를 10-100 μM 농도로 처리할 때 대조구보다 약15-86%로 유의미하게 감소하였으며, 10 μM 농도에서 나타난 RANKL억제 활성은 세포독성과 다른 패턴으로 나타나는 것을 확인하였다. 본 연구결과를 토대로 복령의 균핵 에탄올 추출물이 골모세포 분화를 증진시키거나 억제할 수 있는 가능성을 확인하였으며, 복령의 디클로로메탄 분획물로부터 분리 정제된 dehydrotumulosic acid가 골다공증 예방 및 치료에 도움을 줄 수 있을 것으로 사료된다. Wolfiporia hoelen (Fr.) Y.C.Dai & V. Papp, commonly known a s Poria cocos, is a significant traditional herb used for medicinal and culinary purposes Asian and European countries. Many studies have confirmed that the main components of W. hoelen have pharmacological activities and thatits extract has been shown to affect bone metabolism. This study aimed to the potential of a 50% ethanol extract of the sclerotium of W. hoelen for preventing and treating bone diseases. The ethanol extract was systematically fractionated using n-hexane, dichloromethane, and ethyl acetate. The dichloromethane fraction caused an approximately 29% increase in alkaline phosphatase (ALP) differentiation activity in C2C12 cells compared to the control. Four compounds isolated from this active dichloromethane fraction were identified through instrumental analysis and literature references as 3α-dehydrotrametenolic acid, ergosterol, pachymic acid, and dehydrotumulosic acid. All four compounds were evaluated at increasing concentrations (1, 3, 10, 30, and 100 μM) to determine their effects on ALP differentiation activity in C2C12 cells and RANKL-induced inhibition activity in bone marrow macrophages (BMMs), with a concurrent assessment of cytotoxicity at these concentrations. At a concentration of 3 μM, dehydrotumulosic acid caused a 160% increase in ALP activity, 24% higher than in the BMP-2 control. BMMs treated with dehydrotumulosic acid at concentrations between 10 and 100 μM showed a substantial 15–86% decrease in RANKL-induced inhibition activity compared to the control, with distinct patterns of RANKL inhibition and cytotoxicity observed at 10 μM. These findings suggest that the ethanol extract from the sclerotium of W. hoelen has potential to modulate bone-cell differentiation, while highlighting the possible benefits of dehydrotumulosic acid isolated from the dichloromethane fraction of W. hoelen for preventing and treating osteoporosis.

중광지곡에서 피리 연주의 숨자리 조사

이소라 세계음악학회 1999 음악과 문화 Vol.1 No.-

[토론문] ‘건강장애 학생 대상의 평가 개선에서의 쟁점’에 대한 토론문

이소라 한국학습장애학회 2021 한국학습장애학회 학술대회지 Vol.2021 No.-

열적 특성이 우수한 탄소면상발열체 제조 및 특성 연구

이소라,김원석,이교우,김산나,전관구 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.1

본 연구에서는 얇고 전기전도성이 우수하며 광투과율이 좋은 탄소섬유 발열시트의 제조 및 특성을 조사한다. 탄소섬유 발열시트는 단 탄소섬유를 사용하여 습식법 제조한 부직포 형태이다. 탄소섬유 발열 시트의 높은 전기전도성 확보를 위해 분산과 바인더 역할을 동시에 할 수 있는 유기물을 사용하고 분산성에 대한 전기전도도와 발열특성을 조사한다. 제조된 탄소섬유 발열시트는 잔류 유기물, 표면형상, 강도 등을 TGA, SEM, UTM을 이용하여 확인하고, 균일 크기의 발열시트들의 저항과 인가전압에 대한 발열특성을 적외선 카메라를 이용하여 측정한다. 또한, 투입한 탄소섬유량의 조절을 통한 발열시트의 빛 투과도를 조절하며, 이의 응용으로 개발한 반투과성 발열 유리를 소개한다.

경기도 지역 모심기ㆍ논매기ㆍ지경닺이 노래의 音組織

이소라 한국국악학회 1989 한국음악연구 Vol.17·18 No.-

뇨중 악성종양표지자, Giftec 검사에 관한 연구

이소라,안효준,최재웅,금동극 대한임상검사정도관리학회 1994 臨床檢査와 精度管理 Vol.16 No.1

개상질소리의 유형과 그 전파영역

이소라 국립민속박물관 2013 민속학연구 Vol.0 No.32

A rice-threshing song is a working song sung when getting grains by striking down a rice sheaf into a wood-mortar or on a threshing-stand with a stone or a stump. I used for this paper mainly my materials that I recorded through field-work during 1985-2004 for farming songs in every town in South-Korea and in some districts in North-Korea. There are the Chugnam type, the Gangwon type, the Gyeongsang-do type and etc. in the Korean types of the rice-threshing song. There are the general Chugnam type<score 1> and the Seocheon type in the Chugnam type : The former is sung in the area of its central and western parts in the Province, Chungcheongnam-do. The latter is sung in the some area of Seocheon District situated in the southwestern part of Chungcheongnam-do, and is redivided with the Seocheon-A type and the Seocheon-B type<score 2>. There are the Hongcheon type and the Gangneung type in the Gangwon type : The former is sung in Hongcheon District, and redivided with the Hongcheon-A type(the 'Jajaja' type) and the Hongcheon-B type(the 'Jojojo' type). The latter is sung in Gangneung city and its distinctive word is 'Madaengi'. There are the 'Otdurumi' type, the 'Bongheya' type and the 'Bousse' type in the Gyeongsang-do type. The cultural area of the 'Bongheya' and the 'Bousse' types is a part of the Province, Gyeongsangbuk-do. I refered to the special features of every type.

논맴소리‘대허리’고찰 -대허리 곡의 전파지역, 존재 양상 및 단허리 곡과의 선후관계-

이소라 국립민속박물관 2012 민속학연구 Vol.0 No.30

Daeheori and Danheori are very similar songs for weeding rice paddies. They are sung in call-and response-form, the leader singing changing verses, the chorus in a constant refrain. They are only different from the core-word (Dae or Dan) of the chorus part. Their cultural area is the southwestern Gangwon-Do, the northern Chungcheongbuk-Do, the southeastern Gyeonggi-Do and the eastern Chungcheongnam-Do. Gangwon-Do is the area for Danheori. Chungcheongnam-Do is the area for Daeheori. Chungcheongbuk-Do and Gyeonggi-Do are areas where Danheori and Daeheori exist together. Their general type has each solo part or chorus which is 12/8 times(or inherent one), but there are longer and slower types. An example of the general type's chorus part is : ‘Eowha Eolssin Daeheoriya’ or ‘Eowha Eolssan Daeheoriya’. When we go to the border area of propagation, we meet its mutation types : the Olka type, the Seulseul type, the Gumsil type, the Humcheo type, and the Neomcha type(‘Eowha Neomcha Daeheoriya’). The northern part of Jungwon District keeps the 'Dan' of Danheori inflowed from the cultural center of Danheori, the Wonju and Hwoeongseong area, but it changes into Daeheori in the southcentral Jungwon District which is the cultural center of Daeheori.. We can see the examples that the farmers in Gangwon-Do sang Danheori while weeding rice paddies with the hoe, and sang Banga of the slow tempo or Banga of the fast tempo while weeding rice paddies with bare hands. But the farmers of Jungwon District already sang Banga of the slow tempo while weeding rice paddies with the hoe, and so they seem to sing Daeheori while weeding rice paddies with bare hands. Danheori and Daeheori songs were sung for encouraging workers to continue work with stooping waist. Why did Danheori of Gangwon-Do change to Daeheori in the southcentral area of Jungwon-gun? Did it add the meaning of a big-Heori(waist), because of one leg stretched when weeding rice paddies with bare hands? I feel I need to study more on the language custom of the Jungwon area. I used for this paper mainly my material that I gathered through field-work since 1983 when I recorded especially the songs for weeding rice paddies in every town in South-Korea and folk songs of some districts in North-Korea during that period.

지시적 자서전 프로그램이 노인 부부의 주관적 안녕감 및 결혼만족도에 미치는 영향

이소라,고선규,권정혜 한국임상심리학회 2009 Korean Journal of Clinical Psychology Vol.28 No.4

This study examined how Guided Autobiography affected elderly couples' subjective well-being and marital satisfaction. Guided Autobiography, which is based on the life review concept, includes two components. Participants first write an autobiographical essay on certain themes that have proven to be important in the lives of most people. Then, participants read their essays aloud to a group, to encourage and enrich life review. For this study, we revised the Guided Autobiography for elderly Korean couples. Participants were 43 older adults living in the community ranging in age from 60 to 84. There were 21 in the Guided Autobiography group and 22 in the control group. After the first and the eighth session, both groups filled out questionnaires that included measures of subjective well-being and marital satisfaction. Participants completed the course over 9 weeks, meeting weekly for about 90 minutes. Our analysis indicated that there were significant differences between pre-test and post-test scores for the Guided Autobiography group, whose members demonstrated a significant decrease in negative affect scores and improvement in global marital satisfaction scores. However, there were no significant differences between pre-test and post-test for the control group across all scale scores. Thus, these results suggest that Guided Autobiography may produce positive changes in negative affect and global marital satisfaction for elderly couples.

논김양산도 攷

李素羅 한국민요학회 1999 한국민요학 Vol.7 No.-