산란계 농장에서의 생물학적 위해요인과 관리점 분석

이성모,유한상,홍종해,Lee, Sung-Mo,Yoo, Han Sang,Honsg, Chong-Hae 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.4

An egg has been considered as one of the most important food sources because of it's nutritional superiority and reasonable price. With the complexity of egg flow system from production to consumption in Korea, preventive measures for egg safety have been required. Therefore, our study was carried out to analyze hazards for the egg under farm level and develope preventive measures with a purpose of obtaining egg safety. To analyze biological hazards, microbial contamination of egg(normal, dirty and cracked), water, feed, manure and equipments associated with laying were investigated. One isolate of Salmonella enteritidis and S. bardo were detected from the manure and dirty egg shell respectively. The sanitary conditions in the farm were surveyed by questionaries. Confirmation of vaccination for purchasing chicks and establishment of sanitary guidances for water and type of water suppliers ought to be preceded. Feed supplier systems including feed tanks, feed pipes and hoppers were known that their contamination might give it a chance to infect individuals and egg content and shell. The safe and rapid disposal of dead chickens and rodent were reported as more crucial factors to prevent infectious disease and manage good sanitation. Egg selector and collecting belt should be kept properly not to be contaminated from egg fluids and feces. It should be also considered that regular gathering of eggs, removal of dirty or cracked ones, storage under refrigeration and the use of disposable egg tray were continuously fulfilled. Conclusively, Our results suggested that HACCP-based system for providing fresh and safe eggs to consumers should be applied to the farm.

유 성분 분석을 통한 젖소 영양상태 및 개체관리에 관한 연구

이성모,김동원,최병렬,서강문,홍종해,Lee, Sung-mo,Kim, Dong-won,Choi, Byung-ryul,Seo, Kang-moon,Hong, Chong-hae 대한수의학회 2001 大韓獸醫學會誌 Vol.41 No.2

Milk constituents and somatic cell count (SCC) were analysed for 4,059 milk samples from 178 dairy farms from April to December in 1999. Correlations among each milk constituents, milk urea (MU) concentration and SCC in association with lactation stage, milk yield and parity, and balancing status of nutrients' supplies were analysed, and the results are summarized as follows; Averages of milk fat percent, total solids percent and milk yield were $3.72{\pm}0.91%$, $12.50{\pm}1.31%$ and $23.80{\pm}8.54kg$, respectively, whereas those were significantly lower during the summer season. In contrast, average of MU concentration was $0.0361{\pm}0.0006%$ which was significantly higher during the summer season. With milk yield, concentrations of fat, protein and SCC in milk decreased but concentrations of lactose and urea in milk and body condition score (BCS) were not altered. Concentrations of fat, protein, lactose, total solids, SNF, and urea in milk were significantly affected by stage of lactation ($P{\leq}0.0001$) but SCC was not changed. Parity of dairy cows had a significant effect on concentrations of fat ($P{\leq}0.02$), lactose ($P{\leq}0.0001$), total solids ($P{\leq}0.002$), and SNF ($P{\leq}0.0001$) in milk and milk yield ($P{\leq}0.0005$) but did not change concentrations of urea and protein in milk. Somatic cell count had significant positive correlationship with percentages of fat, protein and total solids ($P{\leq}0.0001$), respectively, but had negative correlationship with percentages of urea and lactose in milk and milk yield ($P{\leq}0.0001$). Milk urea concentration was negatively correlated with concentrations of protein, fat, total solids, and SNF in milk and milk yield ($P{\leq}0.0001$) and, according to regression analysis using milk urea concentration and SCC, following equation was obtained; $Y(MU)=3.688{\times}10^{-2}-4.04{\times}10^{-7}{\times}X(SCC{\times}1,000)(r^2=0.0038$, $P{\leq}0.0001$). We studied balance between protein and energy supplies to dairy cows in each farm based upon milk urea and protein concentrations, and results showed that 137 of total 178 farms fed cows unbalanced amounts of dietary protein and energy.

경인지역의 가축방역 실태조사

이성모 ( Sung Mo Lee ) 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.1

A survey was performed to analyse the current animal disease control system by questionnaires. A questionnaire was administered to the livestock-related experts(n=116) residing in Gyeong-In region and the livestock farmers(n=108) residing in Incheon from June to July in 2007. The data was analyzed statistically by SPSS 12.0 version. First, the offices related to the animal disease control were dispersed and their cooperation was not effective. Second, the scale of local disease centers was weaker than that of central one in both man-power(eg. veterinarians), budget and so on, and was not enough to control the outbreak of the main animal diseases. Third, there were also insufficient incentives for livestock farmers to report notifiable animal disease. Finally, there was limitation in bury area and incineration facilities. To develop domestic animal industry, control of the disease and rearing of the environmentally friendly livestock farm are the main directions in Gyeong-In region, Production of high quality and safe livestock products is the most important factor to expand the livestock industry. Conclusively, local animal disease center with comparison to central one should be expanded to control the disease and develop the livestock industry in Incheon metropolitan city.

계란의 보관방법에 따른 품질 변화

이성모 ( Sung Mo Lee ),김경호 ( Kyoung Ho Kim ),홍종해 ( Chong Hae Hong ) 한국가축위생학회 2002 韓國家畜衛生學會誌 Vol.25 No.1

This experiment was carried out to investigate the changes of egg quality during storage in order to find out the proper storage condition. Fresh eggs were stored at cold condition (5℃, relative humidity; RH: 65±3%) and room condition(25℃, RH: 40±3%) during 10 weeks and examined microbiological quality, weight loss and interior quality periodically. Weight loss was indicated 17.93% at room condition and 2.56% at cold condition. The pH of egg white and yolk were increased from 7.94 to 9.03(average 13.72%) and from 6.16 to 6.57(average 6.65%) at cold condition and from 7.94 to 9.54(average 20.15%) and from 6.16 to 6.90(average 12.01%) at room condition respectively. But pH of egg white and yolk did not showed significant difference between before molting and after molting. Haugh unit was decreased from 82.0 to <30 at room condition after 3 weeks. The most frequent bacteria isolated were E coli, Staphylococcus leutus, S saprophyticus, Citrobacter freundii, Klebsiella oxytoca, Providencia rettgeri, and Pontoea spp. It was recommended that shelf life of unrefrigerated eggs would not be longer than 21 days at room temperature in summer.

액체크로마토그래피를 이용한 벌꿀 중 테트라싸이클린계 항생물질의 정량분석 및 잔류조사

이성모 ( Sung Mo Lee ),박은정 ( Eun Jeong Park ),홍지영 ( Jee Young Hong ),김정임 ( Jung Im Kim ),이정구 ( Jung Goo Lee ),황현순 ( Hyun Soon Hwang ),김용희 ( Yong Hee Kim ) 한국가축위생학회 2005 韓國家畜衛生學會誌 Vol.28 No.3

Oxytetracycline, tetracycline, chlortetracycline and doxycycline in honey were separated by solid phase extraction(SPE) and determined with high performance liquid chromato-graphy(HPLC) with UV/Visible detector. Analysis was carried out using following conditions; XTerra C8 column(3.9 × 150 ㎜ i.d. 5㎛), mobile phase composed of 0.01M oxalic acid : methanol : acetonitrile(820:80:100, v/v/v), isocratic pump at a flow rate of 0.9 ㎖/min. and 50㎕ of injection volume, UV/Visible detector with wavelength of 360㎚. The calibration curves of four tetracyclines showed linearity(γ2>0.999) at concentration range of 100~1,000ng/㎖. The recoveries in fortified honey represented more than 70% with low coefficient of variation(<10%) for concentration range of four tetracyclines. The detection limits for oxytetracycline, tetracycline, chlortetracycline and doxycycline were 13.8, 14.6, 26.2 and 24.9ng/g in acacia honey, respectively. We also monitored tetracyclines residue in domestic honey[n=38, acacia(20), wild flower(18)] and foreign honey[n=22, legally distributed(13), illegally distributed(9)] using modified CharmⅡ screening and HPLC confirmation methods. Seven of the 60 samples(11.7%) were suspect positive using modified CharmⅡ screening test. Chlortetracycline residue was found in one foreign honey(illegally distributed) tested at concentrations of 0.22 ppm. Conclusively, for more effective control of tetracyclines used in beekeeping should be further survey for residues in honey and also national guidelines(maximum residue limit:MRL) and methods should be obligatory.

肛門周圍에 發生한 Squamous cell carcinoma의 治驗例

李成模(Sung Mo Lee),兪熙(Hee Yoo),金光演(Kwang Yun Kim) 대한외과학회 1973 Annals of Surgical Treatment and Research Vol.15 No.5

인천 지역 사육견의 심장사상충 감염률 및 병리조직학적 연구

이성모 ( Sung Mo Lee ),황현순 ( Hyun Soon Hwang ),김종훈 ( Jong Hoon Kim ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.22 No.1

The prevalence of canine heartworm(Dirofilaria immitis) infection in 150 mixed-breed dogs (male: 54, female: 96) from February to December 1997 was investigated by using antigen test kit(ICT GOLD(TM) HW, Synbiotics, USA) based on immunochromatographic assay in Inchon city. Also, gross and histopathological findings of an antigen positive dog were carried out. The results were summarized as follows1. Four dogs were positive from 150 tested dogs(2.7%). They were all more than 2 years old and infection rates in male and outdoor dogs was higher than those in female and indoor, respectively. Species of infected dogs were Pug(2), German Sheperd(1) and Great-dane(1). 2. Regional infection rates were closely related with housing system in the city. 3. Pathological findings of antigen-positive dog was excessive enlargement, congestion and hemorrhage of lung and D immitis in heart and histologically hemosiderin, hypertrophy of pulmonary alveoli wall and irregular hypertrophy of pulmonary artery inner wall. Microfi-laria was observed in pulmonary artery and arteriole, ventricle and splenic artery.

알루미늄 주조/단조 공정에서 주조조직 제어 및 예비성형체 설계에 관한 연구

이성모(Sung Mo Lee),왕신일(Wang Shin Il),김효량(Hyo Ryang Kim),배원병(Won Byong Bae) 한국정밀공학회 1999 한국정밀공학회 학술발표대회 논문집 Vol.1999 No.11월

알루미늄 주조/단조 공정을 이용한 자동차용 에어컨 캠프레서 피스톤의 생산에 관한 연구

이성모(Sung Mo Lee),왕신일(Shin Il Wang),김효량(Hyo Ryang Kim),배원병(Won Byong Bae) Korean Society for Precision Engineering 2000 한국정밀공학회지 Vol.17 No.8

인천지역에서 유통되는 벌꿀의 품질조사

이성모 ( Sung Mo Lee ),홍지영 ( Jee Young Hong ),박은정 ( Eun Jeong Park ),김정임 ( Jung Im Kim ) 한국가축위생학회 2007 韓國家畜衛生學會誌 Vol.30 No.1

A total of 60 samples (38 domestic honeys and 22 foreign honeys) were collected from October in 2003 to May in 2004 in Incheon metropolitan area, and contents of important quality-related components in honeys were analyzed using methods of Korea Food Code. Most of the honeys were satisfied with Korean limit (21%) in moisture content even though 4 samples brought by Korean travellers and 1 sample in domestic honey (wild flower) were higher than the limit. The lowest mean level of moisture in the honeys was imported foreign honeys (16.95%). All of the samples were below of 0.6% in the analysis of ash contents. The highest content was imported foreign honey (0.19%) while the lowest content was in domestic acacia honey (0.05%). Acidity of domestic honeys (9.49-9.94meq/kg) was approximately half of that in foreign honeys. All samples were satisfied with Korean limit (40.0meq/kg). In the analysis of fructose and glucose, only three samples of foreign honeys were lower than Korean limit (sum of both ; 65%). The content of sucrose in all samples was less than 7%. Mean of Hydroxymethylfurfural (HMF) contents were 12.83-24.3mg/kg in domestic honey, and 31.34-45.58mg/kg in foreign honey. However, three samples brought by Korean travellers were not satisfied with Korean limit (<80mg/kg). In conclusion, quality of domestic honey was better than that of foreign honey. Also, it may be needed that the honeys purchased by Korean travellers in foreign countries should be continuously monitored.