Anti-American Sentiments in Korea, 2002-3

이성계 ( Seong Kye Lee ) 고려대학교 동아시아교육연구단 2004 동아시아연구 Vol.8 No.-

홍삼 생약 복합물(KTNG0345)이 섬유아세포의 Procollagen 생합성과 Matrix metalloproteinase-1(MMP-1) 활성에 미치는 영향

소승호,이성계,황의일,구본석,한경호,김나미,So, Seung-Ho,Lee, Seong-Kye,Hwang, Eui-Il,Koo, Bon-Suk,Han, Gyeong-Ho,Kim, Na-Mi 고려인삼학회 2007 Journal of Ginseng Research Vol.31 No.4

본 실험은 홍삼, 사상자, 산수유를 이용하여 주름 예방 효과가 있는 건강기능 식품을 개발하기 위한 기초자료로 활용하기 위하여 개별추출물과 이들의 혼합물 WM1, WM3 (KTNG0345)에 대하여 사람의 섬유아세포를 이용한 세포증식, 콜라겐 생합성 정도, MMP-1 효소활성, 항산화 활성을 조사하였다. 사용된 시료는 $1{\sim}10\;{\mu}g/ml$의 농도로 첨가하여 세포를 배양했을 때 $83{\sim}100%$의 세포증식율을 나타내어 세포 독성을 나타내지 않았으며, 콜라겐 생합성 정도는 대조군이 474.8 ng/ml이었고 retinoic acid는 568.3 ng/ml이었으며, 5 ug/ml의 농도를 기준으로 할 때 홍삼은 533.9ng/ml, 사상자는 539.3 ng/ml, 산수유는 514.1 ng/ml이었다. 홍삼과 생약을 68:232의 비율로 혼합한 WM1은 474.6 ng/ml이었고 홍삼과 생약을 136:164의 비율로 혼합한 WM3는 561.4 ng/ml로 증가하였다. MMP-1활성은 10 ug/ml의 농도에서 홍삼(ER)은 31.9 ng/ml, 사상자(WT)는 32.85 ng/ml, 산수유(WC)는 32.0 ng/ml, WM1은 31.3 ng/ml이었고 WM3(KTNG0345)는 28.85 ng/ml으로 대조군의 88% 수준으로 감소하였다. SOD활성에서는 1000 ug/ml의 농도로 처리하였을 때 65-97% 항산화활성을 나타내었다. 이들의 결과를 종합해 보면 홍삼과 사상자, 산수유 및 이들 혼합물은 human dermal fibroblas에 대하여 세포 독성을 나타내지 않으면서 콜라겐 생합성을 촉진하고 MMP-1의 활성을 억제하였으며 홍삼과 생약 엑기스를 136:164의 비율로 혼합했을 때 그 효과가 증진되었다. WM3 (KTNG0345)는 지표성분으로서 ginsenoside-$Rb_1$, torilin, loganin을 각각 10.85 mg/g, 0.128 mg/g, 3.92 mg/g 함유하였다. Skin wrinkles are associated with collagen synthesis and matrix metalloproteinase-I (MMP-1) activity. This study was carried out to select optimum ratio of 3 herbs in skin health food for anti-wrinkle. Human dermal fibroblast cell was incubated with experimental samples, which were Korean red ginseng ethanol extracts (ER), Torilis fructus water extracts (WT), Corni fructus water extracts (WC) and their mixtures (WM1, WM3). And then we determined effects on collagen biosynthesis, MMP-1 activity and SOD activity in human dermal fibroblast cell. In control group, collagen biosynthesis was amounted at 474.8 ng/ml and 533.9 ng/ml, 539.3 ng/ml, 514.1 ng/ml in ER, WT and WC respectively. Furthermore, WM3 (KTNG0345) was increased to 561.45 ng/ml. MMP-1 activity of ER, WT, WC, WM1 were determined to 31.9 ng/ml, 32.85 ng/ml, 32.0 ng/ml, 31.3 ng/ml and WM3 (KTNG0345) was decreased to 28.85 ng/ml. In addition, the experimental samples showed a antioxidative activities. From this results, we conclude that Korean red ginseng ethanol extracts, Torilis fructus water extracts, Corni fructus water extracts and their mixtures have a anti-wrinkle effect and WM3 (KTNG0345) may be regarded as an optimum composition for synergic effect producing. The standardized components of KTNG0345, ginsenoside-$Rb_1$, torilin and loganin were identified at 10.85 mg/g, 0.128 mg/g and 3.92 mg/g respectively.

홍삼 생약 복합물(KTNG0345)이 섬유아세포의 Procollagen 생합성과 Matrix metalloproteinase-1(MMP-1) 활성에 미치는 영향

소승호(Seung-Ho So),이성계(Seong-Kye Lee),황의일(Eui-Il Hwang),구본석(Bon-Suk Koo),한경호(Gyeong-Ho Han),김나미(Na-Mi Kim) 고려인삼학회 2007 Journal of Ginseng Research Vol.31 No.3

본 실험은 홍삼, 사상자, 산수유를 이용하여 주름 예방 효과가 있는 건강기능 식품을 개발하기 위한 기초자료로 활용하기 위하여 개별추출물과 이들의 혼합물 WM1, WM3(KTNG0345)에 대하여 사람의 섬유아세포를 이용한 세포증식, 콜라겐 생합성 정도, MMP-1 효소활성, 항산화 활성을 조사하였다. 사용된 시료는 1~10 ㎍/ml의 농도로 첨가하여 세포를 배양했을 때 83~100%의 세포증식율을 나타내어 세포 독성을 나타내지 않았으며, 콜라겐 생합성 정도는 대조군이 474.8 ng/ml이었고 retinoic acid는 568.3 ng/ml이었으며, 5 ㎍/ml의 농도를 기준으로 할 때 홍삼은 533.9 ng/ml, 사상자는 539.3 ng/ml, 산수유는 514.1 ng/ml이었다. 홍삼과 생약을 68 : 232의 비율로 혼합한 WM1은 474.6 ng/ml이었고 홍삼과 생약을 136 : 164의 비율로 혼합한 WM3는 561.4 ng/ml로 증가하였다. MMP-1활성은 10 ㎍/ml의 농도에서 홍삼(ER)은 31.9 ng/ml, 사상자(WT)는 32.85 ng/ml, 산수유(WC)는 32.0 ng/ml, WM1은 31.3 ng/ml이었고 WM3(KTNG0345)는 28.85 ng/ml으로 대조군의 88% 수준으로 감소하였다. SOD활성에서는 1000 ㎍/ml의 농도로 처리하였을 때 65-97% 항산화활성을 나타내었다. 이들의 결과를 종합해 보면 홍삼과 사상자, 산수유 및 이들 혼합물은 human dermal fibroblast에 대하여 세포 독성을 나타내지 않으면서 콜라겐 생합성을 촉진하고 MMP-1의 활성을 억제하였으며 홍삼과 생약 엑기스를 136 : 164의 비율로 혼합했을 때 그 효과가 증진되었다. WM3(KTNG0345)는 지표성분으로서 ginsenoside-Rb1, torilin, loganin을 각각 10.85 ㎎/g, 0.128 ㎎/g, 3.92 ㎎/g 함유하였다. Skin wrinkles are associated with collagen synthesis and matrix metalloproteinase-I (MMP-1) activity. This study was carried out to select optimum ratio of 3 herbs in skin health food for anti-wrinkle. Human dermal fibroblast cell was incubated with experimental samples, which were Korean red ginseng ethanol extracts (ER), Torilis fructus water extracts (WT), Corni fructus water extracts (WC) and their mixtures (WM1, WM3). And then we determined effects on collagen biosynthesis, MMP-1 activity and SOD activity in human dermal fibroblast cell. In control group, collagen biosynthesis was amounted at 474.8 ng/ml and 533.9 ng/ml, 539.3 ng/ml, 514.1 ng/ml in ER, WT and WC respectively. Furthermore, WM3 (KTNG0345) was increased to 561.45 ng/ml. MMP-1 activity of ER, WT, WC, WM1 were determined to 31.9 ng/ml, 32.85 ng/ml, 32.0 ng/ml, 31.3 ng/ml and WM3 (KTNG0345) was decreased to 28.85 ng/ml. In addition, the experimental samples showed a antioxidative activities. From this results, we conclude that Korean red ginseng ethanol extracts, Torilis fructus water extracts, Corni fructus water extracts and their mixtures have a anti-wrinkle effect and WM3 (KTNG0345) may be regarded as an optimum composition for synergic effect producing. The standardized components of KTNG0345, ginsenoside-Rb₁, torilin and loganin were identified at 10.85 ㎎/g, 0.128 ㎎/g and 3.92 ㎎/g respectively.

생마늘과 무취마늘의 화학성분의 비교

이종원,이미경,이형옥,이성계,도재호,김만욱 ( Jong Won Lee,Mi Kyung Lee,Hyung Ok Lee,Seong Kye Lee,Jae Ho Do Man Wook Kim ) 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.40 No.5

The purpose of this study was to investigate the differences in proximate composition, free sugars, amino acids, fatty acids and Hunter color values between fresh and odorless garlic. The contents of crude fiber, crude fat and ash in odorless garlic were similar to those in fresh garlic. Sugars and crude protein decreased during manufacture of odorless garlic. Among the free sugars, sucrose, maltose and fructose decreased in odorless garlic, while the content of glucose increased. Total amino acid content decreased by about 30% in odorless garlic. Among the amino acids decreased arginine, isoleucine etc. The contents of linoleic acid, palmitic acid and oleic acid were higher than those of other fatty acids in fresh garlic and odorless garlic, but laruric acid was in little quantity. There were no significant differences in the Hunter L and b value between the fresh and odorless garlic, but value for greeness(-a) was lower in odorless garlic.

홍삼 생약 복합물(KTNG0345)의 피부 주름개선에 관한 작용기전

소승호(Seung-Ho So),이성계(Seong-Kye Lee),황의일(Eui-Il Hwang),구본석(Bon-Suk Koo),한경호(Gyeong-Ho Han),정진호(Jin Ho Chung),이민정(Min-Jung Lee),김나미(Na-Mi Kim) 고려인삼학회 2008 Journal of Ginseng Research Vol.32 No.1

본 실험은 홍삼 혼합물 (KTNG0345)을 이용한 주름 예방 및 개선효과가 있는 건강기능 식품을 개발하기 위한 기초자료로 활용하기 위하여 시료를 경구투여한 무모생쥐의 피부조직으로부터 MMP-3의 발현양상과 작용 메커니즘을 연구하였다. MMP-3의 발현정도는 농도 의존적으로 현저한 감소를 나타내었으며, 유전자와 단백질 모두에서 동일한 양상을 보였다. PAK는 변화가 없었지만, p38, p-p38 그리고 c-Jun, p-c-Jun을 통계적으로 유의하게 감소시킴으로써 MMPs의 발현 감소를 가져온 것으로 보인다. 뿐만 아니라 자외선에 의한 TNF-α의 생성 또는 유입을 억제함으로써 TNF-α receptor에 의해 매개되는 신호전달 경로를 둔화시켜 MMPs의 발현을 감소시킨 것으로 보인다. 이렇게 KTNG0345는 복합적인 활성으로 작용하여 주름생성 억제 활성을 보이는 것으로 판단된다. UV irradiation causes skin-aging involving coarse wrinkles, thickening, dyspigmentation, and rough skin surface. These phenomena in complex skin tissue is controlled with receptor of cell surface growth factor and cytokine receptors. The activation of receptors induces multiple downstream signaling pathways including expression of MMPs (matrix metalloproteinases). This study was aimed to elucidate the mechanism for anti-wrinkle activity of Korean red ginseng, Torilis fructus and Corni fructus mixture (KTNG0345). In this animal study, we have investigated decreasing effects of Korean red ginseng mixture on MMP-3 synthesis through diminishing TNF-α signaling that express MMP-1, -3, and -9. c-Jun and c-fos as a component of transcription factor AP-1 (activator protein-1) were analyzed the expression level using real time PCR and western blotting. c-Jun was decreased dose dependent manner both gene and protein level where as cfos was not changed. In upstream, JNK and PAK was not changed, but p38 was decreased in down stream. MMP-3, final product in this pathway was significantly decreased in dose dependent manner. These results suggest that Korean red ginseng mixture have a anti-wrinkle activity through TNF-α mediated MMPs expression pathway.

한국산 털목이버섯의 영양성분과 수화조건에 따른 특성 변화

이종원(Jong-Won Lee),이성계(Seong-Kye Lee),도재호(Jae-Ho Do) 한국식품과학회 1995 한국식품과학회지 Vol.27 No.5

한국산 및 외국산 홍삼의 사포닌 및 무기물 성분 비교

이종원(Jong-Won Lee),이성계(Seong-Kye Lee),도재호(Jae-Ho Do) 고려인삼학회 2002 Journal of Ginseng Research Vol.26 No.4

This study was carried out to compare the contents of saponins and minerals component in Korean Red Ginseng (Heaven, Earth, Good grade), North Korean Red Ginseng (Heaven, Earth, Good grade), Japanese Red Ginseng (Oonju, Sinju 1, 2, 3 grade, respectively) and Chinese Red Ginseng (Seokju, Gilim, 1, 2, 3 grade, respectively). Crude saponin contents were different on according to the grade and cultivation area, and was 3.05-3.76% in Korean Red Ginseng,2.09-3.21% in North Korean Red Ginseng, 2.82-3.71% in Chinese Seokju Red Ginseng, 2.72-3.62% in Chinese Gilim Red Ginseng, 2.11-2.44% in Japanese Oonju Red Ginseng, 2.18-2.87% in Japanese Sinju Red Ginseng, and the amount of ginsenoside-Rb1, -Re, -Rg₁ in Korean Red Ginseng were higher than those of North Korean, Chinese and Japanese Red Ginseng. The contents of mineral components were similar, but La, Na and Sn component in Korean Red Ginseng showed the higher amount than those of other Red Ginsengs.

수삼 증자 시 생성되는 유출액을 이용한 ginsenoside-Rg₃ 강화 효모 제조

김나미(Na-Mi Kim),이성계(Seong-Kye Lee),조해현(Hae-Hyun Cho),소승호(Seung-Ho So),장동필(Dong-Pil Jang),한성태(Sung-Tai Han),이종수(Jong-Soo Lee) 고려인삼학회 2009 Journal of Ginseng Research Vol.33 No.3

홍삼의 유효성분인 ginsenoside-Rg₃가 강화된 식용 효모를 제조하기 위하여 수삼 증자 유출액을 이용하여 4종의 식용효모를 배양하였다. 효모의 생육도는 Saccharomyces cerevisiae가 가장 높았으며, YEPD 배지에 배양한 경우에 비하여 약 75%의 생육도를 나타내었다. YEPD 배지에 배양한 Saccharomyces cerevisiae에는 ginsenoside가 함유되어있지 않았으나 수삼 증자 유출액에 배양한 Saccharomyces cerevisiae에는 ginsenoside-Rg₃가 0.033 mg 함유되어 있는 것으로 나타났다. 4종의 효모 중 Saccharomyces cerevisiae가 향긋하고 구수한 향취를 나타내어 기호도가 가장 우수하였다. 이러한 결과로 보아 수삼 증자 유출액에 식용 효모를 배양하면 ginsenoside-Rg₃가 강화된 효모를 제조할 수 있으며, Saccharomyces cerevisiae가 생육율과 ginsenoside-Rg₃ 함량이 높고 향미의 기호도가 우수하여 식용 효모로 활용하기에 가장 적합하였다. To produce ginsenoside-Rg3 enriched edible yeast, ginseng steaming effluent (GSE) was used for yeast cultivation in this study. Four kinds of edible yeasts were cultured in sterilized GSE (2% w/v, pH 6.5), without any nutrient, for 48 h at 30℃, and their growth and ginsenoside compositions were determined. Among the yeasts, Saccharomyces cerevisiae showed the highest growth in the GSE medium. 267.1 ㎎ of Saccharomyces cerevisiae biomass was produced from 1 g of GSE solid and ginsenoside-Rg₃ contents was determined with 0.033 mg. Saccharomyces cerevisiae also showed the best overall acceptability, with a herbal and fermentative flavor and a slightly bitter taste. From these data, we conclude that Saccharomyces cerevisiae is the excellent strain for production of ginsenoside-Rg₃ enriched edible yeast using GSE.

Rhizoctonia solani에 의한 인삼 모잘록병 방제용 종자처리 약제 선발

신정섭(Jeong-Seop Shin),이승환(Seung-Hwan Lee),조혜선(Hye-Sun Cho),조대휘(Dae-Hui Cho),김경주(Kyoung-Ju Kim),홍태균(Tae-Kyun Hong),박철수(Chol-Soo Park),이성계(Seong-Kye Lee),정희영(Hee-Young Jung) 한국농약과학회 2015 농약과학회지 Vol.19 No.4

水稻幼苗와 土壤에서 ??C標識 Diazinon의 去就

李成桂,金均,黃乙喆,朴昌奎 서울大學校 農科大學 1985 서울대농학연구지 Vol.10 No.2

The fate of diazinon in the intact rice plants and submerged paddy soil has been investigated with (??) diazinon, The labelled diazinon solution was applied to paddy water and distribution of radioactivities in the rice seedlings, paddy soil, volatile fraction and carbon dioxide has been ascertained at end the of incubation times of 0.5,1,4,6 and 9 days respectively. In addition, extract of plants and paddy soils were subjected to TLC separation for examination of possible transformation products of diazinon. The results may be summarized as follows; 1. Total recoveries of radiactivities were between 57.2~73.6 per cent. 2. Radioactivity in rice seedlings increased with incubation periods reaching one tenth of treated radioactivity at the end of 9 day incubation. 3. Non-extractable radioactivity in paddy soil increased with incubation periods. 4. Radioactive volatile fraction increased in the presence of rice seedlings. 5. Pyrimidinol was unique conversion product of diazinon in rice seedlings and paddy soils. 6. Pyrimidinol applied to paddy soil is readily absorbed by rice seedlings.