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이상철,안병태,박웅양,이승호,노재섭,이경순,Lee, Sang-Chul,Ahn, Byung-Tae,Park, Woong-Yang,Lee, Seung-Ho,Ro, Jai-Seup,Lee, Kyong-Soon 한국생약학회 1994 생약학회지 Vol.25 No.2
A chemical examination of the aqueous acetone extract of the leaves of Securinega suffruticosa has led to the isolation of nine phenolic compounds. On the basis of chemical and spectroscopic evidences, the structures of these compounds were established to be gallic acid(1), corilagin(2), helioscopinin B(3), geraniin(4), bergenin (5), norbergenin(6), 11-O-galloyl norbergenin(7), gallocatechin(8) and rutin(9).
이상철,상균영,구자철,서영호,Lee, Sang-Chul,Xiang, Jun-Yong,Gu, Ja-Chul,Suh, Yung-Ho The Korean Operations Research and Management Scie 2006 經營 科學 Vol.23 No.2
The purposes of this research are to identify causalities among flow and customer loyalty In Chinese online games, and to identify the factors by which flow are influenced. This research tests the model with Chinese on-line game users and compare this result with Korea and Japanese results which were conducted by Lee's research. These implications are thought to be helpful for Korean online game companies to understand the Chinese online game user and to develop the penetration strategies. The results indicated that significant Path coefficients to flow were the convenience of operator, the provision of information, the reality of design. The results indicated that significant path coefficients to customer loyalty were the involvement of virtual community and flow. The involvement of virtual community to flow was not significant but to customer loyalty was significant. The provision of information was negatively influenced on flow. The result of comparison indicated that the path coefficients were different among nations. Korea online game companies need to develop the indigenized online game and to Provide the information to their Chinese partner correctly and quickly.
이상철,이삼선,허민석,최순철,박태원,유동수,Lee Sang-Chul,Lee Sam-Sun,Heo Min-Suk,Choi Soon-Chul,Park Tae-Won,You Dong-Soo 대한영상치의학회 1999 Imaging Science in Dentistry Vol.29 No.1
Purpose: The purpose of this study was to calculate the size and CT number of both normal parotid and submandibular gland. and evaluate their relation to sex, age and obesity using computed tomography. Materials and Methods: The computed tomography was performed parallel to the Frankfurt plane in 46 subjects with healthy salivary gland. The subjects were divided into the three groups (young, middle. old) according to their ages. The size of salivary gland was determined as maximum cross-sectional area and the CT number of salivary gland was determined as the mean CT number of three ROI's. The body mass index was calculated from weight and height. Results: The mean maximum cross-sectional area was 7.79(±1.25)cm² on parotid gland and 4.12(±0.83) cm² on submandibular gland. The mean CT number was -4.43(±23.87) HU on parotid gland and 50.01(±15.63) HU on submandibular gland. There was decreasing pattern of the maximum cross-sectional area of submandibular gland and the CT number of both parotid and submandibular gland according to age(p<0.05). As the body mass index increased. the maximum cross-sectional area of parotid gland increased and CT number of both parotid and submandibular gland decreased(p<0.05). The maximum cross-sectional area of submandibular gland in male was larger than that in female(p<0.05). As the maximum cross-sectional area and CT number of left salivary gland increased. those of right gland increased(p<0.05). Conclusion : Intra-individual differences in salivary gland size and CT number is considered in the age and individual obesity.
염화나트륨 농도가 느타리 자실체의 아미노산과 향기성분에 미치는 영향
이상철,편하영,박윤진,오태석,장명준,Lee, Sang-Chul,Pyeon, Ha-Young,Park, Youn-Jin,Oh, Tae-Seok,Jang, Myoung-Jun 한국버섯학회 2021 한국버섯학회지 Vol.19 No.4
본 연구는 염화나트륨에 의한 비생물적 스트레스가 생육과정 중의 느타리에게 미치는 영향을 자실체의 생육특성, 그리고 구성아미노산함량과 향기패턴 분석을 통해 확인하였다. 농도별로 염화나트륨을 처리한 톱밥배지에서 수확한 느타리 자실체의 생육특성을 조사한 결과, 처리구별 수량은 무처리구 대비 염화나트륨 0.5%처리구에서의 값은 비슷했으며, 염화나트륨 처리 농도가 1.0%에서 2.0%으로 증가하면서 수량이 확연히 감소하였다. 자실체의 구성아미노산함량 분석결과, 무처리구 대비 모든 염화나트륨 처리구에서 glutamic acid과 proline을 제외한 aspartate, threonine, serine, glycine, alanine, methionine, valine, isoleucine, leucine, phenylalanine, tyrosine, lysine, histidine, arginine의 14종 구성아미노산의 함량이 낮음을 확인하였다. 자실체의 향기패턴 분석결과, 염화나트륨 처리구에서 버섯고유의 향을 나타내는 octane compound로 추정되는 물질의 intensity가 감소하였음을 chromatography상에서 확인하였다. We investigated the effect of sodium chloride-associated abiotic stress on the development of Pleurotus ostreatus. We examined the growth characteristics of fruiting bodies, constituent amino acids, and fragrance pattern to determine the effect of culturing Pleurotus ostreatus on a sawdust substrate supplemented with sodium chloride in a dose-dependent manner. Pleurotus ostreatus fruiting bodies exhibited an increasing tendency towards augmented yields when grown in the presence of 0.5% sodium chloride as compared with that grown in the control group. However, increasing the supplementation of sodium chloride from 1.0 % to 2.0% resulted in significantly decreased yields of Pleurotus ostreatus fruiting bodies in these groups as compared with that in control groups. Further assessment revealed the presence of 14 types of amino acids in the fruiting bodies, including aspartate, threonine, serine, glycine, alanine, methionine, valine, isoleucine, leucine, phenylalanine, tyrosine, lysine, histidine, and arginine, at lower levels in all the sodium chloride-treated groups than in the control group; except for glutamic acid and proline. Similarly, fragrance pattern analysis of the Pleurotus ostreatus fruiting body by chromatography confirmed that the intensity of the substances presumed to be octane compounds, to which the unique flavor of mushrooms is attributed, was lower in all the sodium chloride-treated groups than in the control group.
이상철,김유삼,Lee, Sang-Chul,Kim, Yu-Sam 생화학분자생물학회 1985 한국생화학회지 Vol.18 No.3
오리의 uropygial gland와 간에서 $poly(A)^+mRNA$를 분리하여 그들의 in vitro translation mixture로부터 pI 6.6인 glucose-6-phosphate dehydorgenase를 분리 하고 in vivo isotope labeling된 같은 효소를 분리하여 SDS polyacrylamide disc gel electrophoresis를 통하여 비교하였다. 그 결과 in vitro translation mixture로 부터 분리한 효소와 in vivo labeling된 효소는 그것이 uropygial gland의 것이거나 간의 것이거나 모두가 uropygial gland에서 직접 정제한 효소와 같은 크기임을 알 수 있었다. 이러한 사실은 uropygial gland에 존재하는 pI 6.6인 glucose-6-phosphate dehydrogenase가 갖는 효소적 특성은 소수의 아미노산의 차이에 기인하는 것으로 예측된다. One glucose-6-phosphate dehydrogenase with pI of 6.6 was isolated from in vitro translation mixture and was compared with in vivo isotopically labeled enzyme by SDS polyacrylamide gel electrophoresis. The molecular size of the enzymes, isolated by antibody prepared against the purified enzyme and protein A agarose, are very similar to each other. This result suggests that the specific catalytic properties of the enzyme from the uropygial gland may depend on the minor difference in amino acid sequence.
이상철,김유삼,Lee, Sang-Chul,Kim, Yu-Sam 생화학분자생물학회 1985 한국생화학회지 Vol.18 No.2
오리의 uropygial gland로 부터 pI 6.6인 glucose-6-phosphate dehydorgenase를 순수하게 정제하여 효소활성과 기질 및 보조효소와의 결합에 관여하는 아미노산에 대해 조사하였다. Cysteine의 -SH기에 특이하게 반응하는 p-chloromercuriphenylsulfonic acid, 5,5'-dithio-bis-(2-nitrobenzoic acid), N-ethylmaleimide와 arginine의 guanidino기에 특이하게 반응하는 phenylglyoxal 등이 효소의 활성을 억제한다는 사실로 보아 효소의 active site나 그 근처에 cysteine과 arginine이 존재하는 것으로 예상된다. 또한 glucose-6-phosphate가 존재할 때 이 reagent들에 의한 효소의 활성억제에 아무런 영향이 없었으나 $NADP^+$존재하에서는 효소의 활성억제 현상이 크게 감소한다는 사실로 미루어 cysteme과 arginine이 $NADP^+$와 효소가 결합하는 위치냐 또는 그 부근에 있는 것으로 판단된다. $Mg^{++}$이 존재할 때 p-chloromercuriphenylsulfonic acid, 5,5'-dithio-bis(2-nitrobenzoic acid)와 phenylglyoxal에 의한 효소의 활성 억제가 더 크게 나타났는데, 이러한 사실은 EDTA와 $Mg^{++}$이 공존할 때 이 reagent에 의한 활성 억제가 EDTA가 존재하지 않을 때 보다 감소하였다는 것으로 보아 $Mg^{++}$이 이 reagent에 의한 효소의 활성 억제를 증가시킨다는 사실을 확인하였다. Glucose-6-phosphate dehydrogenase from the uropygial gland of duck was purified in an electrophoretically homogenous form and its essential amino acid for catalysis was investigated. Three -SH group directed reagents, p-chloromercuriphenylsulfonic acid, 5,5'-dithio-bis-(2-nitrobenzoic acid) and N-ethylmaleimide, inhibited the enzyme activituy, and arginine directed reagent, phenylglyoxal, also inhibited the enzyme activity. The inhibition of the enzyme by these reagents was protected by addition of $NADP^+$ whereas it was not affected by that of glucose-6-phosephate indicating that essential -SH and guanidino group are located on the $NADP^+$ binding region of active site. $Mg^{++}$ promoted the inhibition of the enzyme by the -SH and guanidino group directed reagents. But the promotion of the inhibition was considerably recovered in the presence of EDTA. This results suggested that divalent metal cation may affect the conformational change toward the susceptible one against modifying reagents.
치자(梔子)가 JNK와 NF-kB를 통한 항산화와 항염증의 대사과정에 미치는 영향
이상철,심성용,김연섭,Lee, Sang-Chul,Sim, Sung-Yong,Kim, Youn-Sub 대한한방안이비인후피부과학회 2016 한방안이비인후피부과학회지 Vol.29 No.2
Objectives : The purpose of this study is to observe the effects of Gardeniae Fuctus(GF) on the metabolic process of antioxidant and anti-inflammation. Methods : 4-HNE was injected into PC-12 cell to cause oxidative stress-induced inflammatory response, and then a western blot was conducted to observe the expression of Nuclear Factor-kB (NF-kB) and c-Jun N-terminal kinase (JNK) protein that are important factors involved with inflammation. Results : 1. The Gardeniae Fuctus water extract 50 ㎍ and 100 ㎍ significantly suppressed the increase in JNK protein expression in PC-12 cell. 2. The Gardeniae Fuctus water extract 50 ㎍, 100 ㎍ and 200 ㎍ significantly suppressed the increase in NF-kB protein expression in PC-12 cell. Conclusion : These results suggest that the Gardeniae Fuctus water extract has antioxidative and anti-inflammatory activity through suppressing the activity of JNK and NF-kB.
심리적 유인과 사이트 품질, 공동체의식이 온라인게임에 미치는 영향
이상철,김남희,문재영,서영호,Lee, Sang-Chul,Kim, Nam-Hee,Moon, Jae-Young,Suh, Young-Ho 한국경영정보학회 2003 Asia Pacific Journal of Information Systems Vol.13 No.4
The purpose of this research is to identify if psychological temptation, site quality and sense of community influence user's flow and addiction and to identify causalities among flow, addiction, customer satisfaction and customer loyalty in online industry. Many previous researches in the area of online games have been carried out about addiction by psychologist and about the development of related technologies by scientists. There are only a few studies about the customer satisfaction from the online business perspective. However, this research is different from the previous ones in the sense that both flow and addiction are considered in the study of the relationship between customer satisfaction/loyalty and flow/addiction in the area of online industry. The empirical results of high-order factor analysis indicate that six independent variables such as design, information, feedback, impulsiveness and motivation have converge three second-order variables such psychological temptation, site quality and sense of community. Consequently, site quality and sense of community have impacts on the flow, while on the other hand, psychological temptation has impacts on the addiction. Conclusively, customer satisfaction and loyalty are positively related not with the addiction but with the flow. Besides, customer loyalty is significantly influenced by the flow and the customer satisfaction. This indicates that companies in the online game industry have to develop a strategy for the flow which is more socially and ethically allowable than the addiction.