백혈병 T-임파구에서 새로이 분리한 Leucine zipper 단백질의 분자생물학적 연구

李尙九 한남대학교 1997 국내석사

電力系統에서의 高調波 現象에 관한 調査 硏究

李相九 漢陽大學校 産業大學院 1983 국내석사

Plantlet Regeneration from Protoplasts of Ailanthus altissima

李相求 경북대학교 1989 국내석사

가중나무(Ailanthus altissima Swingle)의 野外個體로부터 器內誘導, 培養된 callus와 植物體를 材料로하여 原形質體를 分離 培養한 바 다음과 같은 結果를 얻었다. 1. 가중나무의 形成層組織으로부터 callus誘導는 2,4-D 1.0 mgl^-1을 添加한 MS 基本培地에서 가장 良好하였다. 2. 器內에서 誘導, 增殖된 callus와 葉肉組織으로부터 原形質體를 分離하기 爲한 最適 酸素溶液은 Cellulase 1.5%, Hemicellulase 0.5%, Macerozyme 0.5%, Driselase 0.5% 그리고 Pectolyase 0.05%와 Mannitol 0.6M(callus), 0.7M(葉肉組織)이 添加된 溶液에서 各各 3時間, 8時間 동안 培養했을 때 最高의 原形質體 收率을 얻을 수 있었다. 3. 分離된 原形質體를 水洗 精製한 後 여러 가지 培養要因에 따라 培養하였을 때 培養方法은 liquid plating法이, 原形質體의 培養密度는 5×10⁴과 10^5 ml^-1, 渗透壓 調節劑의 影響은 callus原形質體는 mannitol 0.2M + glucose 0.4M에서 葉肉組織 原形質體는 glucose 0.2M + sucrose 0.4M에서 細胞分裂率과 colony 形成率이 가장 좋았다. 그리고 培養培地에 따라서는 callus 原形質體의 境過 細胞分裂과 colony 形成은 KM8P 培地에서 callus의 生長은 MS 基本培地에서 良好하였으며, 葉肉組織 原形質體의 培養도 거의 類似한 結果를 얻었다. 4. 原形質體 培養 8 週後에 callus 原形質體로부터 callus를 얻을 수 있었으며 이들 callus를 2,4-D 1.0 mgl^-1 + BAP 0.1 mgl^-1를 添加한 培地에서 6 週間 培養한 後 再分化培地로 移植하였을 때 BAP 1.0 mgl^-1가 含有된 MS 基本培地에서 가장 많은 再分化된 植物體를 얻었다. 5. 再分化된 植物體는 植物 生長調節 物質이 添加되지 않은 MS 基本培地와 1/2 MS 培地에 移植하였을 때 發根되어 正常的인 生育을 하였다. Plant regeneration from cambium callus protoplasts was obtained in Ailanthus altissima Swingle. Four enzyme mixtures were evaluated for the isolation of protoplasts from cambium callus and leaf mesophyll of in vitro cultured tree of heaven. An enzyme mixture of 1.5% w/v Cellulase, 0.5% Hemicellulase, 0.5% Macerozyme, 0.5% Driselase and 0.05% Pectolyase gave the highest protoplast yield. One of the most important steps in the isolation and culture of protoplasts was maintaining the osmotic potential of the enzyme solution at or near the isolation level of the tissues during the digestion of the cell wall and for the incubation on the culture medium. Culture methods used for protoplasts were liquid plating, liquid drop, hanging drop and agarose plating, with the most effective method being liquid plating for both cell division and colony formation. Protoplasts were isolated from both types of tissue of A. altissima divided rapidly and formed colonies on KM8P medium supplemented with 1.0 mgl^-1 2,4-D and 0.1 mgl^-1 BAP. But callus growth of protoplast derived from callus was enhanced on MS medium contained 1.0 mgl^-1 2,4-D and 0.1 mgl^-1 BAP. The presence of 0.1 - 1.0 agl-1 BAP was most effective in shoot induction from protoplast-derived callus at a frequency of 14-38%. While high cytokinin concentrations and the presence of 2,4-D on medium promoted callus growth. When induced shoots were transferred to MS or half-strength MS medium without growth regulators, all shoots were successfully rooted.

헥사플루오르프로필렌을 함유하는 불소계 공중합체의 합성 및 박막의 광학특성

이상구 충북대학교 2013 국내박사

PVDF and P(VDF-co-HFP) were successfully prepared from free radical solution polymerizations using diisopropyl peroxidicarbonate (DIPPDC) in the presence of 1,1,2-trichlorotrifluoroethane (CFC-113). The reactivity ratios of VDF and HFP were estimated as rVDF=2.06 and rHFP?0. This result indicates that HFP cannot undergo self propagation. The weight-average molecular weight and molecular weight distribution of copolymers were found to decrease with increasing HFP content. The melting temperature of copolymers linearly decreased with the increase of HFP content because of the introduction of HFP. Moreover, no melting peak was observed for the copolymers with high HFP content. The glass transition temperature of copolymers gradually increased with the increase of HFP content because of restriction of flexibility of the polymer. PVDF and P(VDF-co-HFP) were spin-coated on the glass slides in order to fabricate thin film with a thickness of 1 mm approximately. It was found that the surface morphology of fluorinated thin films was strongly dependent upon the crystallinity of polymers. In addition, the surface morphology was the most important factor to determine the optical transmittance of glass coated with the fluoropolymer thin film. As decreasing the crystallinity of the polymer by introducing hexafluoropropylene with a bulky CF3 moiety, the surface of thin film became flattened and the direct transmittance of visible light was increased by reducing scattering. PTrFE and P(TrFE-co-HFP) were characterized by various instrumental techniques. The maximum incorporation of HFP was 19.6 mol%. It was found that the reactivity ratios of TrFE and HFP were rTrFE=4.12 and rHFP?0, respectively, by applying Mayo-Lewis method. It was also found that the incorporation of HFP in copolymer chain influenced thermal properties. As increasing HFP content in copolymers, the melting temperature and crystallinity of copolymers were decreased and the glass transition temperature of copolymers gradually shifted to low temperature. Reduced crystallinity of copolymers was helpful to prepare coating layers having much even surface. Compared with coating layer prepared from TrFE homopolymer, the surface roughness was decreased and the direct transmittance through the coating layer increased when the copolymers of TrFE and HFP were used. This tendency was more appreciable as increasing HFP content in the copolymers. 불소계 공중합체인 P(VDF-co-HFP)는 저온 개시제 DIPPDC와 불소계 용매 CFC-113을 사용해서 합성하였다. Mayo-Lewis식이 적용된 단량체 반응성비의 계산결과, rVDF=2.06과 rHFP?0으로 확인되었다. 이러한 결과는 HFP의 자가 사슬 성장이 거의 일어나지 않는다는 것을 나타낸다. 고분자의 중량평균 분자량, 분포도 및 수율은 HFP 함량이 증가할수록 점차적으로 감소하였다. HFP의 자가 사슬 성장이 거의 일어나지 않는 반응에서, HFP 함량의 증가는 전체 반응성을 감소시키기 때문에 수율 및 중량평균 분자량이 낮아진 것이다. 고분자의 녹는점 및 결정화도는 HFP 함량이 증가할수록 선형적으로 감소되었다. 더욱이 HFP 16.99 mol% 이상에서는 녹는점이 발견되지 않았다. 이러한 결과는 HFP가 PVDF의 결정화를 방해하기 때문에 나타나는 것이다. 고분자의 유리전이온도는 HFP 함량이 증가할수록 고분자 사슬의 유동성이 감소되어 점차적으로 상승하였다. PVDF와 HFP 11.03 및 16.99 mol %를 포함하는 P(VDF-co-HFP) 박막의 광 투과율을 조사하였다. 벌크한 CF3 그룹은 가지고 있는 HFP가 고분자의 결정화를 방해하는 인자로 작용하고 있다는 것을 ATR-IR 분석을 통해 확인하였다. 표면형상과 광 투과율의 관계를 관찰하기위해, 슬라이드 글래스에 스핀 코팅된 박막은 200 ℃에서 천천히 냉각시키면서 결정화되었다. 고분자의 HFP 함량증가는 박막의 표면을 균일하게 하고 정 투과율을 증가시켰다. 또한, 결정성 박막의 정 투과율은 고분자의 굴절률과 같은 고유특성보다 표면형상에 의해, 더 큰 영향을 받는다는 것을 확인하였다. 불소계 공중합체인 P(TrFE-co-HFP)는 저온 개시제 DIPPDC와 불소계 용매 CFC-113을 사용해서 합성하였다. Mayo-Lewis식이 적용된 단량체 반응성비의 계산결과, rTrFE=4.2와 rHFP?0으로 확인되었다. 이러한 결과는 상기에서 언급했던 것처럼 HFP의 자가 사슬 성장이 거의 일어나지 않는다는 것을 의미한다. 고분자의 중량평균 분자량 및 수율은 HFP 함량이 증가함에 따라 감소하였다. 고분자의 녹는점 및 결정화도는 HFP 함량의 증가와 함께 감소하였다. 또한, 고분자 사슬에 HFP의 결합은 구조적 유연성을 증가시켜 유리전이온도를 감소시켰다. 합성된 고분자의 광학적 특성은 슬라이드 글래스에 스핀 코팅 한 후 가시광선의 광 투과율을 통해 관찰하였다. 고분자 박막의 광 산란은 HFP 함량이 증가함에 따라 감소하였고, 결과적으로 광의 정 투과율은 증가하였다. 이러한 현상은 HFP 함량의 증가로 고분자 박막의 표면 거칠기가 감소하기 때문에 나타난 결과이다.

아르곤 이온 레이저 조사로 확산화염에서 얻어지는 광신호의 해석

이상구 단국대학교 교육대학원 2011 국내석사

동축류 프로판 확산화염에 488 nm 파장의 아르곤 이온 레이저를 조사하는 경우에 발생하는 신호의 특성을 PMT와 monochromator를 사용하여 실험적으로 알아보았다. 파장 515 nm에서 측정되는 광신호에 대한 레이저 polarization, 수광부 편광필터의 존재 유무 및 LII 간섭 신호의 영향을 평가하여 다음을 알 수 있었다. 매연입자가 존재하지 않는 지역에서의 515 nm에서 측정된 신호는 모두 PAH LIF 신호로 해석할 수 있다. 그러나 매연입자가 존재하는 영역에서는 레이저의 polarization과 수광부 편광필터의 사용 유무에 따라 Mie 산란신호와 LII 신호의 영향이 나타났다. 수광부에 수평방향의 편광필터가 없는 경우 얻어지는 신호는, 레이저의 polarization이 수직일 경우에는 대부분이 Mie 산란신호이며 polarization이 수평일 경우에는 multiple scattering이 의심된다. 매연입자가 존재하는 영역에서는 레이저 polarization이 수평이고 수광부에 수평의 편광필터가 장착된 조건에서도 515 nm 파장의 신호가 여전히 나타나며 이는 대부분이 LII 신호로 판단된다. 따라서 PAH와 매연입자가 동시에 존재하는 경우, LII 신호의 간섭이 나타나고 PAH LIF 신호 측정의 어려움이 예상된다.

自閉兒童父母의 스트레스 解消에 관한 硏究

이상구 漢城大學校 2001 국내석사

It is serious problem that parents feel stress from their child. Specially, if the child has a handicap, the problem is more. It is highly possible that the stress of parents have a bad influence on the rehabilitation of the handicapped child and it can break their domestic happiness. It is a hard fact that the parents of an autistic child experience more stress than the parents of other handicapped child. Because the autism appears 2 or 3 years after birth, the parents expected a normal child have definitely shocked than the parents of other handicapped child. In the past, we've studied the autistic child placing emphasis on education for remedy. But we've taken the first step approaching in the aspect of the social welfare, since the category of the handicapped included the autistic child in the welfare law, revised January 1, 2001, for the disabled. So, this research is a help to the remedy for the autistic child and the sound family as analyzing the stress which the parents experience by their autistic child to economic, educational, social and psychological aspect and showing some solutions. First of all, the parents experience economical stress were to be clear in the lower income bracket. Apart from the difficulty of domestic economy, it is were serious stress to the parents that there's not any basic systems of government for its solution. Accordingly, the practical support is to be necessary. Many parents just let their autistic child alone without thinking of the special education for them, as the expense for rehabilitation is so much. But the government have to expand the social support for that child and make the autistic child be educated without any burden in spite of their low income. In case of preschooler, the support is urgent because the early education at the time of finding the autism can minimize the handicap and maximize the effect of social adaptation. Then, it is necessary for the parents experience educational stress to be educated with foundation knowledge of the autism and the ability to deal with that child. There are so many cases that most of parents just think their child for native and let alone though they become find the autism of their child. Really the parents need to learn the foundation knowledge of the autism and the ability to deal with that child through social welfare facilities in advance. In addition to that, middle and high school students also have to learn the common sense of the handicap in the curriculum. Also, it can be a solving method the parents' stress that the autistic child stands for himself through a hiring policy for the handicapped child with vocational education and development and grows as an adaptable child to society. And the social stress mostly begins at the parents' isolation because of their autistic child. For solving this stress, the parents must be able to enjoy their social life through actively participating the social meetings, to settle their stress by organizing the group of the parents with the same handicapped child as Self-Help Group and to share knowledge helping the remedy and rehabilitation of the autistic child. To the end, it's the psychological stress. It is necessary to activate the counsel program for the parents which have the autistic child. Accordingly, the special counsel program as individual counsel, group counsel, family therapy, psychotherapy have to be developed soon. It can be also a cause of stress that there's no consultation office. Actually, it's not familiar for the parents to visit at the consultation office for themselves. So, the active counsel is essential by establishing the consultation office attached to the special education center for the autistic child.

흰띠명나방(Spoladea recurvalis)의 생물적 특성과 비트에서의 요방제수준

이상구 全北大學校 2011 국내박사

The Hawaiian beet webworm (Spoladea recurvalis) is one of the serious insect pests on red beet (Beta vulgaris var. conditiva) in Korea. Biological characteristics, seasonal occurrence and control thresholds on red beet of S. recurvalis had been studied. The mean widths of head capsule of the 1st instar to the 5th instar were 0.204, 0.341, 0.578, 0.890 and 1.363mm, respectively. Growth ratio of each instar were not significantly different among 2nd to 5th instar were 1.67, 1.69, 1.54 and 1.53, respectively. The logarithm of the larva head capsule width was regressed as a function of the stage number of insects, the calculated regression line is highly correlated (Log Y = 1.1184 + 0.2065X, r2 = 0.9975) and Dyar’s constant(K) was 1.61. Leaf areas consumed of each larvae during 24hr at 25℃ were 0.7, 2.2, 6.1, 15.1 and 27.2cm2, respectively. The development, adult longevity and fecundity were studied at various constant temperatures, 15.0, 17.5, 20.0, 22.5, 25.0, 27.5, 30.0, 32.5 and 35.0℃, with 65±5% RH and a photoperiod of 16L:8D. Duration from egg to pre-adult was 51.0 days at 17.5℃ and 14.6 days at 35.0℃ suggesting that higher the temperature, faster the development. Developmental threshold and effective accumulative temperatures of the total immature stage were 10.4℃ and 384.7 degree days, respectively. The nonlinear shape of temperature related development was well conformable to the Lactin model. The normalized cumulative frequency distributions of developmental period for each life stage were fitted to Weibull function with r2 > 0.63. Adult longevity became shorter as the temperature increased from 15.6 to 5.4 days. The female longevity was longer than that of the male. Total number of eggs laid per female varied from 11.4 to 121.9. Net reproductive rate(RO) was highest with 53.5 at 25.0℃. Intrinsic rate of increase per day(rm) and finite rate of increase per day(λ) were highest with 0.16 and 1.17 at 30.0℃, respectively. Doubling time(Dt) was shortest with 4.46 at 30.0℃. Mean generation time(TO) was 14.8 at 35℃. Seasonal occurrence of adult appeared mid July and peaked late August in 2010. Damages of leaves were 71.6% at 3 weeks after planting in 50 larva inoculation plot. The linear relationships between the densities of larvae and the yield reduction were as following; Y = 0.5288X + 1.4427 (r2 = 0.9922). Based on these results the economic injury level was 12.2 larva per 10 plants. The control thresholds calculated by 80% level of economic injury level was 9.8 larva per 10 plants.

韓國 複合運送의 現況과 活性化 方案에 관한 硏究

이상구 東亞大學校 經營大學院 1996 국내석사

Nowadays the overall shipping industry in Korea has hard times due to decreased cargo volume, increased number of competitors and wage increases. Furthermore, the entry of foreign shipping companies and agents into the domestic markets is expected to bring unprecedented change considering our weak circumstances of capital, professional manpower, infrastructure, intermodal transport and total information system. Under these circumstances, it is imperative to secure policies to establish efficient M. T. systems and to develop international M. T. to strengthen international competitiveness and to be able to meet growing import/export volumes and diverse needs of customers. Multimodal transport means the carriage of goods by at least two different modes of transport on the basis of a multimodal transport contract from a place in one country at which the goods are taken in charge by the multimodal transport operators to a place designated for delivery situated in different country. This brings economic benefits to the cargo owner, shipper, transport forwarder and air freight companies and strengthens the international competitiveness of our export/import products and maximize distribution efficiency. Furthermore. strengthening of our international competitiveness would be ultimately depend on the reduction of distribution cost of various goods and jong-hap-mul-ryu system through early establishment of container I. T. system through early establishment of container I. T. system as our country's economic growth is highly dependent on overseas trade. Multimodal Transport System in the form of door to door service links the individual transports on the land, sea and air from origin point to final destination with speed, safety and economy. Consequently, land bridge service system plays an important role in transport of container cargo and has been rapidly developing and spreading all over the world because it has brought considerable economic benefits and efficiency. The following solutions may be suggested. For company managerial issues. First, expansion and specialization of forwarding firms. The only way to secure external competitiveness is to provide quality services based on credibility by increasing the firms' own capital & specialization of operations in order to defeat undercapitalization Second, establishment of Intermodal Transport System. In other words, ring about superior competitiveness & cost saving through efficient connetions between sea/inland/air transport modes by establishment of intermodal transport systmes to satisfy the shippers' demand of for door-to-door transpory. Third, training of professional manpower. Considering possible problems pertaining to international trade, specialization of manpower is imperatice to prevent possible losses during the interpretation & application of numerous related laws & trade codes. Fourth, expansion of overseas marketing network. Establishment of international service organizations and improvement of international competitiveness of our country's M. T. system, which is restricted in its overseas cargo collection activites. And institutional solution suggestions would call for swift porgress of simplication & liberalization of customs clearance porcedures. boo-doo-jik -tong-kwan of export/import cargos & activation of boo-ddo-sae-woon- song stystem, implementation of compensatory fee system for collection cargo, activation of railroad transpory, expansion of inland container/clearace deport, expansion of port facilities & establishment of total M. T. information system. Our country is now facing new challenges under rapidly changing international trade environment by strengthening of regional protectionism such as EU unification, apperance of NAFTA and also UR & demand for open market by developed contries. Under these difficult circumstances it would be necessary to strengthen international competitiveness of export/import cargo and reduce costs by establishment of comprehensive cargo distribution system through progress and effective development of schemes for international M. T.

Monte Carlo simulation기법을 이용한 폐기물매립지 온실가스 배출량 불확도 분석에 관한 연구

이상구 釜慶大學校 大學院 2006 국내박사

학습자 중심 문학 교육 방안 연구

이상구 한국교원대학교 1998 국내박사