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이정은,이규리,남정아,김애선,이소영,이민상,김낙원,Yue Yin,박지원,박성영,정지훈 한국고분자학회 2018 Macromolecular Research Vol.26 No.12
Despite its high therapeutic potential, the use of siRNA has still been limited mostly due to the lack of a proper delivery system. Small number of charges and inflexible structure of siRNA are the major factors that affect cellular delivery efficiency of conventional lipid and polymeric carriers. In this study, we synthesized a 2-dimensional (2D) carrier for siRNA using a facile method to functionalize and stabilize the surface of graphene oxide (GO) with catechol-derivatized poly(2-dimethylaminoethyl methacrylate) (C-p(DMAEMA)). The resulting GO/C-p(DMAEMA) can interact with siRNA on the flexible 2D surface of the GO, wrapping up siRNA molecules by folding to form a dim sum-like nano structure. GO/C-p(DMAEMA) demonstrated significantly improved cellular uptake and enhanced gene silencing efficiency in the presence of serum protein, compared to unmodified GO and bPEI 25 kDa.
이민상,이규리,남민우,정경민,이정은,김낙원,Yue Yin,임수연,유다은,이정미,정지훈 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.65 No.-
The inherent vulnerability of protein biologics to high temperature is the origin of their limited use in clinical settings where proper storage and handling systems are not available. Herein, we demonstrate the first application of natural deep eutectic solvents (NADES) as a temperature-stable storage medium for a real therapeutic protein, human interferon-alpha 2 (IFN-α2). A formulation of IFN-α2 based on a NADES composed of choline chloride and fructose at a 1:1 molar ratio (CCl:Fru) had no influence on the activity of IFN-α2. The NADES provided an appropriate environment for effective protection of IFN-α2 from thermal denaturation at mid (37 °C) and high (70 °C) temperatures during long-term (90 days) and short-term (2 h) storage. Protein stability in the presence of CCl:Fru was demonstrated by orthogonal or complementary methods, including in vitro activity assay, circular dichroism spectroscopy and extrinsic fluorescence spectroscopy. In view of their biocompatibility, customizable properties, and the simplicity of synthesis and use, NADESs are suggested as a green and improved short-term and long-term storage medium that could facilitate the development of room-temperature biologics.