발생중인 병아리의 사이뇌에서 NPY 양성반응세포의 분포

이미영,박일권,이경열,권효정,송치원,박미선,이강이,하권수,김원식,김무강,Lee, Mee-Young,Park, Il-Kwon,Lee, Kyoung-Youl,Kwon, Hyo-Jung,Song, Chi-Won,Park, Mee-Sun,Lee, Kang-Iee,Ha, Kwon-Soo,Kim, Won-Sik,Kim, Moo-Kang 대한수의학회 2002 大韓獸醫學會誌 Vol.42 No.1

This study deals with the distribution and morphological study of the neurons with the neuropeptide Y (NPY) in developing chick brain. The developing brains of Korean native chicks at embryonic days 8 (E8), E10, E12, E14, E16, E18, and E20 were used. The chicks were perfused with 4% paraformaldehyde through the left ventricle and aorta. The brains were removed and transferred into 30% sucrose, and then cut in a cryostat into $60{\mu}m$ in thickness. The sections were immunostained with free-floating and avidin-biotin complex (ABC) methods. The numerous neurons with NPY were first observed in nucleus rotundus of diencephalon at E8. In particular, neurons in nucleus rotundus had the well-developed processes. At E12, the neurons with NPY were distributed widely in diencephalon; nucleus septalis lateralis, medialis, nucleus magnocelluaris preopticus dorsalis, medialis, ventralis, nucleus preopticus medialis, nucleus dorsolateralis anterior thalami pars magnocellularis, and nucleus paraventricularis magnocellularis (paraventricular nucleus) except nucleus rotundus. From E12 to E20, positive neurons were oval-shaped, changed gradually into the spherical- and multipolar-shaped. The shapes of processes were also omnidirectional and the number of those were less than in telencephalon. As the chicks developed, the morphology of neurons with NPY showed the tendency to increase in their sizes and numbers.

멀티미디어 통합 정보검색 프레임워크를 지원하는 OODBMS

이미영,채미옥,박순영,이규웅,김완석,김영준,Lee, Mi-Yeong,Chae, Mi-Ok,Park, Sun-Yeong,Lee, Gyu-Ung,Kim, Wan-Seok,Kim, Yeong-Jun 한국정보처리학회 2000 정보처리학회논문지 Vol.7 No.2

As the multimedia application services have been getting various in Internet, need of multimedia database management system which can manage various multimedia information effectively has been increased. The multimedia application generally needs the features to store, manage, retrieve the multimedia data. We need a database management system framework that may be easily integrated with these features. This paper proposes th database management system framework providing the easy integration with various multimedia information retrieval. The framework uses OQL as the retrieval interface, so that user can use it easily. It can be integrated dynamically and easily with various multimedia retrieval method as a loosely coupled system. In addition there is no performance degradation caused in a loosely coupled system.

전호(前胡)의 기원 판별을 위한 형태특징과 PCR 분석

이미영,주영승,김홍준,고병섭,Lee, Mi-Young,Ju, Young-Seung,Kim, Hong-Jun,Ko, Byoung-Seob 한국한의학연구원 2003 한국한의학연구원논문집 Vol.9 No.1

In the present paper, morphological characteristic and PCR analysis were carried not to identify the medicinal origins of Peucedanum decursivum and Anthriscus sylvestris. Some samples in Korean. markets were Peucedanum decursivum misused with rhizomes of Anthriscus sylvestris. The main. origin of Peucedanum decursivum producted was quite different from those of Anthriscus sylvestris in the morphological characteristics. And PCR analysis, the three primer has developed into anew tool for identification of Peucedanum decursivum and Anthriscus sylvestris.

KSLA 연구논문 - 박물관내 도서관 운영 현황 및 전문도서관으로의 발전 전략

이미영,Lee, Mi-Yeong 한국전문도서관협의회 2010 KSLA bulletin Vol.2 No.-

박물관내 도서관은 인력, 예산, 조직내 인식 부족 등으로 본연의 기능을 전문적으로 수행하지 못하였다. 박물관내 도서관이 전문도서관으로서 보다 발전하기 위한 전략을 해외사례에서 찾고자 하였다. 그 결과, 관리자에서 수집가로서 사서의 역할 변화, 특성화된 컬렉션 확대, 기관 아카이브의 기능 수행, 도서관간 협력 네트워킹 구축 등을 발전전략으로 제시하였다. The lack of human resource, budget and understanding in organization didn't allow the library in museum to accomplish its function fully. I tried to find the development strategies toward special library in abroad cases. As a result, I suggested the librarian's role change from manager to collector, the expansion of the specialized collection, the function as institutional archives, the construction of networking between libraries.

정상 성인에서 복부 드로우-인 기법을 동반한 복부근 활성화가 파악력에 미치는 영향

이미영,Lee, Mi-Young 대한물리치료학회 2012 대한물리치료학회지 Vol.24 No.1

Purpose: The grip strength is influenced by various factors, such as position of the upper extremity, characteristics of the hand, and general physical condition. In this study, we investigated whether abdominal muscle activity in combination with the abdominal drawing-in maneuver has any effect on the grip strength in healthy young adults. Methods: This study included 31 healthy subjects (16 males, 15 females). We used surface electromyography and pressure bio-feedback unit for this experiment. All Subjects were placed in a cock-lying position with comfort and the grip strength was measured. On the following day, the pressure bio-feedback unit was placed beneath their fifth lumbar vertebra, and the, grip strength was measured again. This time, the measurement was taken while drawing-in their abdomen below the navel gently and gradually, while maintaining a neutral pelvic position. Results: The grip strength was significantly increased when subjects performed the drawing-in maneuver than when they were comfortable (p<0.05). In addition, activations of the rectus abdominal muscles significantly increased (p<0.05). Conclusion: In the current study, we found that abdominal muscle activity, in combination with the abdominal drawing-in maneuver, affected the grip strength, positively. Therefore, we suggest that this result should be considered when evaluating the grip strength.

MnO₂ 첨가에 따른 무연 Bi(Na, K)TiO₃-SrTiO₃ 세라믹스의 유전 및 압전 특성

이미영,류성림,류주현,정광현,정영호,홍재일,윤현상,Lee, Mi-Young,Ryu, Sung-Lim,Yoo, Ju-Hyun,Chung, Kwang-Hyun,Jeong, Yeong-Ho,Hong, Jae-Il,Yoon, Hyun-Sang 한국전기전자재료학회 2004 전기전자재료학회논문지 Vol.17 No.10

In this study, 0.96B $i_{0.5}$($Na_{0.84}$ $K_{0.16}$)$_{0.5}$Ti $O_3$ + 0.04SrTi $O_3$ + 0.3 wt% N $b_2$ $O_{5}$+0.2 wt% L $a_2$ $O_3$ + xwt % Mn $O_2$ were investigated as a function of the amount of Mn $O_2$ addition in order to improve dielectric and piezoelectric properties of Lead-free piezoelectric ceramics. With increasing the amount of Mn $O_2$ addition, the density, electromechanical coupling factor( $k_{p}$), piezoelectric constant( $d_{33}$, $g_{33}$) and curie temperature (Tc) showed the maximum value of 5.7 g/㎤, 38 %, 219 pC/N, 26 mVㆍm/N and 32$0^{\circ}C$ at 0.1 wt% Mn $O_2$ addition, respectively, and mechanical quality factor( $Q_{m}$ ) showed the maximum value of 158 at 0.3 wt% Mn $O_2$ addition.ddition.ion.n.

한국산 무우로부터 Far Migrating Anionic isoperoxidase A3 의 정제 및 성질

이미영,김승수 ( Mi Young Lee,Soung Soo Kim ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.4

A far migrating anionic isoperoxidase, named A₃, was isolated from Korean radish (Raphanus sativas L.) root. Purification of the enzyme was accomplished by CM-cellulose chromatography, DEAF-Sephacel chromatography and Sephadex G-75 gel filtration. The enzyme was a glycoprotein having molecular weight of approximately 50,000 as determined by SDS-PAGE and Sephadex G-150 gel filtration and the pI value was 3.0. The optimum pH of the enzyme was 6.0 for guaiacol and H₂O₂and the Km values for guaiacol and H₂O₂ were 8 mM and 1 mM, respectively. Substrate studies with natural phenolic compounds were performed and their Km values were determined. In comparison with other Korean radish isoperoxidases, A₃ had higher affinity for esculetin. A particular interest is that the enzyme showed as sigmoidal substrate saturation curve against scopoletin despite of its single Subunit structure.

아가리쿠스로부터 분리한 $\beta$-glucan과 그 올리고당류의 HT-29 인체 대장암 세포에 대한 항암 활성에 관한 연구

이미영,김기훈,김예운,장헌길,이동석,Lee, Mi-Young,Kim, Ki-Hoon,Kim, Yea-Woon,Chang, Hun-Gil,Lee, Dong-Seok 한국미생물학회 2006 미생물학회지 Vol.42 No.4

칡을 첨가하여 배양한 아가리쿠스버섯(Agaricus blaxei Murill)으로 부터 열수추출, gel filtration chromatography, DEAE ion exchange chromatography를 통하여 아가리쿠스 $\beta$-glucan (AG)을 추출하였다. 추출한 아가리쿠스 $\beta$-glucan에 Bacillus megaterium 유래의 endo-$\beta$-(1$\rightarrow$6)-glucanase를 처리하여 올리고당류(AO)를 얻었다. 이렇게 얻은 AG와 AO를 이용하여 HT-29 인체 대장암 세포에 대한 항암 활성을 조사한 결과, 암세포의 성장 억제 효과는 시료의 처리 농도에 의존적으로 증가하였으며, apoptosis assay에서 암세포의 apoptosis 유발이 농도에 의존적으로 증가되었다. 또한, 암세포의 세포 주기를 분석한 결과, apoptosis 발생을 뜻하는 G0 (sub-G1)기와 G1기의 비율이 증가한 반면 S기와 G2/M기는 대조군에 비해 감소되었다. 이러한 결과를 바탕으로 암세포의 apoptosis 증가에 대한 AO의 작용이 어떤 유전자와 연관이 있는지를 알아보기 위하여 cDNA microarray를 통해 유전자의 발현율을 검색한 결과, apoptosis의 내부 외부 경로에 영향을 주는 유전자(TNESE9, TNFRSF9, FADD, CASP8, BAD, CRADD, CASP9 등)의 발현이 증가되었고 세포 분열 주기의 진행과 관련된 유전자(CCND2와 CDK2)의 발현이 감소되었으며, 세포 분열 주기를 지체시키는 유전자 (CDKN2A)의 발현은 증가되었다. 또한, 사이토카인을 암호하는 유전자 (IL6, IL18, IL6R 등)와 tumor suppressor와 관련된 유전자 (CEACAM1, TP53BP2, IRF1및 PHB)의 발현이 2배 이상 증가된 것을 확인할 수 있었다. 따라서 HT-29 인체 대장암세포에 대한 AO의 성장 억제 작용은 G0/Gl기를 지체시켜 암세포 증식을 억제하고 apoptosis에 의해 암세포를 사멸시키는 항암 활성을 나타내는 것으로 확인되었으며, 특허 AO가 AG보다 현저한 활성을 보였다. 더 나아가 아가리쿠스 $\beta$-glucan (AG)과 올리고당류 (AO)는 항암 활성을 가진 대체 의약 소재로 개발될 수 있을 것으로 기대된다. [ $\beta$ ]-Glucans (AG) were prepared from Agaricus blazei cultured in the medium fortified with the roots of Pueraria spp. by repeated extraction with hot water, gel filtration chromatography and DEAE ion exchange chromatography. Oligosaccharides (AO) were derived from the hydrolysis of AG by an endo-$\beta$-(1$\rightarrow$6)-glucanase from Bacillus megaterium. The anti-HT-29 human colon cancer activity of AG or AO was investigated using MTT assay, apoptosis assay, cell cycle analysis, and cDNA microairay. AG and AO both inhibited proliferation and growth of HT-29 cells, and stimulated apoptosis of the cells in a dose-dependent manner. In cell cycle analysis, treating HT-29 cells with AG or AO resulted in the increase of cells in the G0 (sub-G1) and G1 phase. Especially, AO was more effective in inducing G0/G1 cell cycle arrest than AG. To screen the genes involved in the increase of apoptosis, the gene expression profile of the HT-29 cells treated with AO was examined by cDNA microarray. While several genes involved in cell cycle progression (CCND2 and CDK2) were down-regulated, many genes involved in apoptosis (TNFSF9, TNFRSF9, FADD, CASP8, BAD, CRADD, CASP9 etc), cell cycle inhibitor (CDKN2A), immune response (IL6, IL18, IL6R etc), and tumor suppressor (CEACAM1, TP53BP2, IRF1, and PHB) were up-regulated. These results suggest that AO could inhibit the proliferation and growth of HT-29 cells by G0/G1 cell cycle arrest and induction of apoptosis.

형태특징 및 RAPD분석에 의한 후박(厚朴) 약재의 진위감별

이미영,김홍준,주영승,고병섭,Lee, Mi-Young,Kim, Hong-Jun,Ju, Young-Seung,Ko, Byoung-Seob 한국한의학연구원 2003 한국한의학연구원논문집 Vol.9 No.2

For identification of genuine and misuse samples of Hu-Bak medicines, an morphological characters, and RAPD (random amplified polymorphic DNA) were performed. In this study, three samples were collected, of which two were genuine and one was misuse sample. Genuine samples of 'Hu-Bak' were Magnolia obovata Thunberg and Magnolia officinalis Rehder et Wilson. Misuse samples was Machilus thunbergii S. et Z. In the morphological characters, M. obovata Thunberg and M. officinalis Rehder et Wilson were similar, but M. thunbergii S. et Z. was different with cortex, cambium, and fibrous layer. The result of RAPD analysis, seven primers can distinguish between genuines and counterfeit samples of Hu-Bak medicines.

소음성 난청 선별검사에 HHIE-S(Hearing Handicap Inventory for the Elderly-Screening version)의 적용

이미영,서석권,이충원,Lee, Mi-Young,Suh, Suk-Kwon,Lee, Choong-Won 대한예방의학회 1996 예방의학회지 Vol.29 No.3

이 연구는 1994년도 5월부터 9월까지 동산병원 건강 관리과에서 소음특수건강진단을 받은 근로자 중 일부를 대상으로 하여 1차 선별 순음청력검사에 난청선별 설문지(HHIE-S)의 적용가능성을 점검해 보기 위하여 시행되었다. 대상자는 이 기간중의 특수검진 수진자들로서 총 6,700명 중 계통적 표본추출법에 의하여 매 5번째의 근로자가 추출되었다. 최종분석 대상은 자료가 미비한 14명을 제외한 1,019명으로 남자 488명, 여자 531명이었다. 소음성 난청의 1차 선별검사로 순음청력검사와 HHIE-S를 포함한 설문지검사를 실시하였다. 청력검사의 1차 선별기준은 양쪽 어느 귀의 청력손실이 4000Hz 에서 40dB이상인 자로 하였고 2차 정밀검사에서 난청의 기준은 3분법으로 계산하여 30dB 이상이면서 4000Hz에서 50dB 이상의 청력손실이 있는 자로 하였다. 설문지의 신뢰도는 0.84였다. 청력장애와 관련된 변수들의 단일변량분석에서 유의한 차이를 보인 항목은 남자에서 HHIE-S 총척도와 사회적/상황적 소척도에서 근무기간, 군복무력, 1000Hz 및 4000Hz에서의 청력역치였으며 여자에서 총척도와 소척도 모두에서 연령, 근무기간, 1000Hz 및 4000Hz에서의 청력역치였다. 다단계 다중회귀분적에서 남자에서 1000Hz및 4000Hz에서의 청력역치가 선택되었으며 여자에서는 총척도와 사회적/상황적 소척도에서 1000Hz 및 4000Hz에서의 청력 역치, 근무기간, 연령이 선택되었다. 2차 순음청력검사를 황금기준으로 하여 1차 선별청력검사와 HHIE-S점수를 기준으로 한 ROC곡선을 그린 결과, 최적의 타당도는 병행검사에서 HHIE-S점수 8을 기준으로 하였을 때였으며 민감도와 특이도가 각각 85%와 67%이었으며 위음성이 15%로 최소였다. 이러한 결과는 HHIE-S가 우리나라에서 신뢰도 및 타당도가 비교적 적절하며 소음성 난청 1차 선별검사에 순음청력검사와 함께 병행해서 사용하면 민감도를 높이고 위음성을 떨어뜨림으로써 순음청력검사의 병행검사로 적용가능성이 있음을 시사해 준다. The study was conducted from May to September in 1994 to investigate applicability of the Hearing Handicap Inventory for the Elderly-Screening version(HHIE-S) in parallel with the pure-tone audiometer to the initial screening test of noise-induced hearing loss(NIHL) in some noise-exposed workers. Subjects were selected by systemic sampling that took every 10th person from 6, 700 workers taking the annual occupational health examination by the department of Health Maintenance of Dongsan Hospital Keimyung University in Taegu. The authors administered the pure-tone audiometric test and self-reported questionnaire of HHIE-S including items of sociodemographic and job-related variables concurrently. The final subjects analysed were 1,019(488 males and 531 females) excluding fourteen persons who had many missing values in their questionnaires. The reliability coefficients of HHIE-S scale by Cronbach's alpha were 0.84. In the univariate analysis of hearing handicap measured by the HHIE-S, work duration, military service and the hearing threshold loss at 1KHz and 4KHz by the initial audiometer were significant in males while age, work duration and hearing threshold loss at 1KHz and 4KHz were significant in females. In the stepwise linear regression analysis, hearing threshold loss at 1KHz and 4KHz, was the only selected variable explaining the hearing handicap in males and hearing threshold loss at 1KHz and 4KHz, age, and work duration were selected in females. In ROC curves for HHIE-S scores against NIHL as gold standard which was defined by the follow-up audiogram as more than 30dB of the average of 0.5/1/2KHz and 50dB at 4KHz, the optimal cutoff for the parallel HHIE-S appeared to be 8. The results suggest that HHIE-S appeared to have some reliability and validity in this data and might be used in screening NIHL in parallel with pure-tone audiometer in noise-exposed workers.