폐흡충의 이배체와 삼배체 vasa 유전자 분석 및 특징

이근희,유학선,허재원,유성숙,최선희,박상균,이선주,정동일,공현희,옥미선,정해진,Lee, Keun-Hee,Yu, Hak-Sun,Hur, Jae-Won,Yu, Sung-Suk,Choi, Sun-Hee,Park, Sang-Kyun,Lee, Sun-Joo,Chung, Dong-Il,Kong, Hyun-Hee,Ock, Mee-Sun,Jeong, Hae-Jin Korean Society of Life Science 2007 생명과학회지 Vol.17 No.4

폐흡충은 국내를 비롯한 아시아에서 폐흡충증을 일으키는 중요한 기생충이다. 이러한 폐흡충은 이배체 와 삼배체가 국내에 알려져 있다. 이배체 폐흡충은 양성생식을 하고 삼배체 폐흡충은 단위생식을 하는 것으로 알려져 있다. 그러나 이러한 원인에 대하여서는 알려진 바가 없다. 생식유전자 중 초파리에서 최초 분리되었고 포유동물에서도 그 기능이 밝혀진 vasa 유전자가 가장 유명하다. 이 유전자는 생식세포의 분화에 관여하며 종에 따라서는 정자생성에도 관여하는 것으로 알려져 있다. 이번 연구는 삼배체 폐흡충의 단위생식과 vasa 유전자와의 관계를 규명해 보고자 하였다. 폐흡충의 이배체와 삼배체 성충으로부터 vasa 유전자 전체의 염기서열을 얻을 수 있었다. 두 염기서열의 경우 8개의 ATP-binding domain이 관찰되었고 helicase가 결합할 것으로 예상되는 RGG motif도 관찰되었다. 총 622아미노산 서열로 구성될 것으로 보이며 이배체는 69.018 kDa, 삼배체는 68.930 kDa 크기의 단백질을 만들 것으로 예상되었다. Vasa 재조합 단백질은 GST와 fusion되어 93 kDa 크기에서 관찰되었다. mRNA의 발현은 이배체에 비해 삼배체가 다소 높았다. Anti-Pw-VASA항체를 이용한 면역조직화학법을 수행한 결과 이배체와 삼배체는 다른 기관에서는 면역반응력을 보이지 않고 고환에서만 면역반응력을 볼 수 있었다. 결과를 종합해 보면 vasa 유전자는 이배체 삼배체 모두 가지고 있었으며 정상적으로 발현되었다. In this study, we isolated, characterized, and compared the vasa homologous genes of diploid and triploid Paragonimus westermani and localized VASA homologous proteins in both lung fluke types. Open reading frames of Pw-vasa-2n and Pw-vasa-3n were of 1812 bp, and encoded deduced proteins of 622 amino acids with calculated molecular weights of 69.0 kDa and 68.9 kDa and pI's of 9.11 and 9.03, respectively. A comparison of these two VASA deduced protein sequences showed that only 6 of the 622 amino acids differed. The deduced sequences of Pw-VASA-2n and Pw-VASA-3n contained eight consensus sequences characteristic of the DEAD-box protein family and their N-terminal regions contained four arginine-glycine-glycine (RGG) motifs. These two lung fluke VASA-like proteins were more similar to those of other VASA proteins than to those of other DEAD-family proteins isolated from several organisms (planarian, zebra fish, mouse, and human). vasa homologous gene transcription and VASA protein expressions in triploid type lung flukes was slightly stronger than in the diploid type. Immunostaining showed that testes and a portion of the ovaries of both diploid and triploid lung flukes reacted strongly to anti-Pw-VASA antibody.

Phylogeny of Mite Taxa (Acari : Sarcoptiformes) Based on Small Subunit Ribosomal RNA Sequences

이근희,유학선,박상균,이선주,이경아,김선미,옥미선,정해진,Lee Keun Hee,Yu Hak Sun,Park Sang Kyun,Lee Sun Joo,Lee Kyeong Ah,Kim Sun Mee,Ock Mee Sun,Jeong Hae Jin Korean Society of Life Science 2006 생명과학회지 Vol.16 No.1

We analyzed the phylogenic relationships of 23 partial 18S rDNA sequences of 22 species (1 species has 2 strains) belonging to Sarcorptiforms include 4 new sequences, using several tools. Although geographic distributions are quite far from, sequence similarity of two strains of Dermatophygoides pteronyssinus isolated from Japan and New Zealand were very high. This result suggests that mite migration by animals including human occurred in the two continents. We investigated the Endeostigmata taxonomic relationship between the Prostigmata and Oribatida subgroups using small fragments (340-400 bp) of their 185 rDNA sequences. But Endeostigmata was not grouped with Oribatida or Prostigmata. In conclusion, it is first reported phylogenic relationship for classified mites included in Sarcoptiformes using 185 rDNA sequence analysis and its system is a very powerful tool for classification of mites.

후각 정보의 부호화 방법

이근희,이상욱,김응수,Lee, Keun-Hee,Lee, Sang-Wook,Kim, Eung-Soo 한국정보통신학회 2007 한국정보통신학회논문지 Vol.11 No.7

최근 스마트 모바일 기기의 급속한 보급 및 무선 네트워크의 실용화에 따라 사용자가 언제 어디서나 편리하게 사용할 수 있는 컴퓨팅 환경의 제공이 가능해졌고 이에 따라 차세대 컴퓨팅으로서 휴대형 PC가 실용화될 전망이다. 차세대 휴대형 PC에서는 지금까지의 시각, 청각에 의한 인터페이스 외에도 촉각, 후각, 미각과 같은 인간의 오감을 통한 실감형 서비스를 제공할 수 있는 인터페이스가 등장할 것으로 예상된다. 따라서 본 연구에서는 사용자 중심의 실감형 인터페이스를 구현하기 위하여 인간의 오감 가운데 기억과 밀접한 관계가 있는 후각정보를 기반으로 이를 표현하고 재생하는 기술에 관하여 살펴본다. With the rapid spread of smart mobile products and practical use of wireless network, it became possible to offer the computing environment people can use anytime anywhere and it is predicted that portable computer as the next generation computing is to be put to practical use. This computer will be expected to have the new interface which provides realistic service through human's five senses as well as the interface through the visual and auditory senses. Accordingly, in this study, we are going to talk about the technology that expresses and reproduces what is based on olfactory information - closely related with memory among honan's five senses - in order to embody the lifelike user interface.

등위 접속 명사구의 일치 비대칭 문제 - 허사 there 구문과 관련하여 : 등위 접속 명사구의 일치 비대칭 문제

이근희(Keun-hee Lee) 한국영미어문학회 2007 영미어문학 Vol.- No.82

In English, verbs that occur after conjoined subjects are required to be plural. However, the verbs that occur before the subject nouns seem to have more flexibility in agreement. In this paper, by reviewing Sch?tze"s (1999) processing effect, which is suggested to argue that singular agreement with plural associates in expletive constructions conforms to the grammar and is not the result of a virus, I"d like to show that asymmetry like this can be accounted for as grammatical phenomenon rather than superficial one. And then I tried to find a solution to the asymmetry problem by using the syntactic operation through Agreement in Chomsky (2000) and by applying Ninuma & Park"s (2003) analysis, which shows the asymmetry between Move and Agreement.

거주자우선주차제(주차문화시범지구)와 효율적 시행과 관리를 위한 주차전산관리시스템 개발

이근희(Lee Keun-Hee),금기정(Kum Ki-Jung),이홍범(Lee Hong-Bum) 대한토목학회 2000 대한토목학회논문집 D Vol.20 No.3D

공기 유동장내 액적의 미립화 기구에 관한 실험적 연구

이근희 ( Keun Hee Lee ),김사엽 ( Sa Yop Kim ),이창식 ( Chang Sik Lee ) 한국액체미립화학회 2007 한국액체미립화학회 학술강연회 논문집 Vol.2007 No.-

충북지역 공립단설유치원의 건축계획적 특성에 관한 연구

이근희(Lee, Keun-Hee),이덕희(Lee, Duck-Hee),김종도(Kim, Jong-Do),김기수(Kim, Gi-Soo) 대한건축학회 2017 대한건축학회 학술발표대회 논문집 Vol.37 No.1

There are 23 independent public kindergartens registered in the Chungbuk Office of Education, except kindergartens attached to elementary schools, since the objectives of study and education have been changed based on "nuri course of study" - the course of study established by the government to be applied to children at the age of three to five. Unlike the kindergarten attached to elementary school, the independent public kindergarten requires the change of internal space by diversified leaning and teaching and the change of space organization by the number of pupil in light of his or her age. The purpose of this study is to suggest more efficient space for the room layout by analyzing common use space from the viewpoint of registered architect who takes part in the plan for the layout of external common use space for the course of study and effective use of the space.

생산성논집 : 기업동태가 노동생산성, 고용 및 부가가치 변화에 미친 영향: 2006-2012

이근희 ( Keun Hee Lee ) 한국생산성학회 2014 生産性論集 Vol.28 No.3

We have estimated the contribution of firm dynamics to the aggregate laborproductivity in the Korean economy during 2006-2012 through the Dynamic Olley PakesDecomposition. The contribution of within effect to the growth of aggregate labor productivity is on average estimated as 48.5 percent, and the contribution of covariance effect as a reallocation effect is estimated as 49.4 percent in economy-wide. Especially the reallocation effect is considerable, so we can guess that the resource reallocation has been well operated, to some extent, during the second half of 2000s. In other words it shows that the production resource like as labor seems to have moved to the higher productivity firms or sectors. The covariance effects are relatively high in ICT-Producing sector such as electronic components, computer and communication equipment, as well as Motor vehicles, Financial institutions etc. Moreover the contribution of entry effect is estimated as -16.3 percent, and the contribution of exit effect is estimated as 18.5 percent in economy-wide. Thus the contribution of continuous firms to the growth of aggregate labor productivity is much higher than that of entry effect as well as exit effect. These results are estimated not from the establishment level data but from firm level data, and can be confirmed in the other micro level productivity analyses as well. What the entry effect is estimated as negative means that the levels of labor productivity of entrants are lower than that of continuous firms, so it tells us that the entrants are not as competitive or innovative as continuous firms, contrary to expectation, in terms of technical efficiency. Simultaneously what the exit effect is estimated as positive means the lower productivity firms than continuous firms have been exited from markets, so they contribute positively to the improvement of industry productivity. Meanwhile the contribution of continuous firms to the growth of value-added is 81.8 percent, and the contribution to the growth of employment is 65.0 percent in economy-wide. This situation has been similarly found in manufacturing and service. Therefore we have to highlight the contribution of continuous firms to the growth of productivity, value-added and employment in the short run. Moreover we need to maintain efficiently ‘creative destruction process’ of J. Schumpeter in market in the long run. It is essential that we should induce the creation of employment and the growth of productivity through not only making more competitive firms entered but also having more firms survived in a market at least.

연료 제트의 두 액적간의 충돌기구에 관한 실험적 연구

이근희 ( Keun Hee Lee ),김사엽 ( Sa Yop Kim ),이창식 ( Chang Sik Lee ) 한국분무공학회 2008 한국액체미립화학회지 Vol.13 No.4

갑상선절제술을 시행 받은 고령의 갑상선암 환자의 임상적 특징에 대한 고찰

이근희(Keun Hee Lee)전학훈(Hak Hoon Jun),김종우(Jong Woo Kim),김승기(Seung Ki Kim),허진형(Jin Hyung Heo) 대한갑상선-내분비외과학회 2016 The Koreran journal of Endocrine Surgery Vol.16 No.4

Purpose: This study analyzes the clinical characteristics of thyroid cancer patients over 70 years of age following thyroidectomy. We identified the differences among previous studies and investigated the significance of thyroidectomy for elderly thyroid cancer patients. Methods: Information was abstracted from the charts of fifty-six patients treated during the period of Jan. 1995 to Dec. 2015. The abstraction included gender, age, surgical method, location with size of the main lesion, extent of lymph node metastasis, pathological features, gene mutation, complication, stage and most recent visit. Results: Our study showed a frequent occurrence (96.4% of the study group) of papillary thyroid cancers in elderly patients. There were three cases of postoperative complications. None had recurrence, and there was only one death due to complications of pneumonia. There were 11 advanced cases of cancer stage IV. Conclusion: Previous studies show that postoperative prognosis of elderly thyroid cancer patients is favorable. Average life expectancy is rising in South Korea, and therefore, the number of elderly thyroid cancer patients is increasing. Our study is meaningful in that it confirms the results of previous studies by surveying elderly patients over a 20 year period. It is deemed necessary that thyroidectomy be performed on elderly patients.