http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
Customized N-glycosylation for the production of glucocerebrosidase in Arabidopsis
이균오,Ki Seong Ko,Jae Yong Yoo,Bích Ngọc Thị Vũ,Ji Ye Park 한국당과학회 2021 한국당과학회 학술대회 Vol.2021 No.01
Plants and plant cells are evolving with improved safety and production as attractive options for biopharmaceutical production. A significant barrier to the development of biopharmaceuticals in plants, however, lies in the fact that plant-derived N-glycans contain plant-specific sugar residues such as β1,2-xylose and α1,3-fucose bound to the pentasaccharide core (Man3GlcNAc2) as well as β1,3-galactose and α1,4-fucose involved in the formation of Lewis a (Lea) epitope that may cause allergic reactions in humans. Additionally, sugar residues such as α1,6-fucose, β1,4-galactose, and α2,6-sialic acid, which are believed to play important roles in biopharmaceutical action, storage, distribution, and half-life, are missing from the naturally occurring N-glycans in plants. To use plant cells as a means of producing biopharmaceuticals, it is essential to produce plants which contain N-glycan compatible with biopharmaceuticals. However, the structure of N-glycans appears to be related to hormone signalling and how the structure of N-glycans altered during glycoengineering influences plant production is still uncertain. Here, we suggest a strategy for producing customized N-glycans in plants and the related technological barriers.
옥수수 성 결정 메커니즘: 세포 사멸, 세포 방어, 세포주기 멈춤
김종철,이균오,Kim, Jong-Cheol,Lee, Kyun-Oh 한국생명과학회 2006 생명과학회지 Vol.16 No.4
옥수수(Zea mays)는 단성화 식물로서 암꽃과 수꽃이 한 식물체내에 분리되어서 존재하며 수정시 이질성을 높이는 방향으로 진화되었다. 암꽃과 수꽃 각각은 단성화 상태로 분화하기 전에 한 개의 암술과 세 개의 수술 원시세포가 동일하게 형성된다. 옥수수가수꽃으로 분화할 때는 암술 원시세포에서 세포사멸 현상이 일어나는데 이것은 TASSELSEED 유전자들에 의해 매개된다. 이와 대조적으로 암꽃의 암술에서는 TASSELSEED 유전자들에 의한 세포사멸이 억제되는데 여기에는 SILKLESS1 유전자가 관여한다. 한편, 암꽃의 수술에서는 세포주기 멈춤 현상이 오랜 시간 지속되다가 결국에는 수술이 죽게 된다. 이때 세포주기를 조절하는 유전자인 CYCLIN B 와 WEE1 유전자가 이 과정에 참여한다. 이와 더불어, 지베렐린 생합성의 시간적 공간적 조절이 수술의 세포주기 멈춤의 원인이 된다. 본 총설에서는 옥수수의 성 결정 과정 중에 일어나는 세포사멸, 세포 방어, 세포주기 멈춤에 대하여 분자세포 발생 생물학 및 유전학적인 견지에서 고찰하였다. Maize (Zea mays L.) is a monoecious plant, which separates male (tassel) and female (ear) floret that evolved into increasing heterogeneity. In each floret, male or female, bears both one pistil and three stamens primodia before diverged to unisexual state. When diverged to tassel, pistil cell death occurs in the pistil primodium, which is mediated by TASSELSEED genes. In contrast, cell protection occurs in the ear pistil from TASSELSEED-mediated cell death, which is mediated by SILKLESS1 gene. On the other hand, cell cycle arrest occurred for a long time in the ear stamens and then the stamens eventually dye. The cell cycle regulating genes such as CYCLIN B and WEE1 are involved in this process. Furthermore, the temporal and spatial regulation of gibberellin biosynthesis may cause cell cycle block in arresting stamen cells. This review describes the cell death, cell protection, and cell cycle arrest mechanism during maize sex determination process at the molecular, cellular and developmental biology, and genetic levels.
RNA-Dependent RNA Polymerase 6 Is Required for Efficient hpRNA-Induced Gene Silencing in Plants
RIKNOHARMOKO,이균오,Wahyu Indra Duwi Fanata,유재용,고기성,임영길,엠다나짐우단,Tri Agus Siswoyo,이승식,김둘이,이상열 한국분자세포생물학회 2013 Molecules and cells Vol.35 No.3
In plants, transgenes with inverted repeats are used to in-duce efficient RNA silencing, which is also frequently induced by highly transcribed sense transgenes. RNA silencing induced by sense transgenes is dependent on RNA-dependent RNA polymerase 6 (RDR6), which con-verts single-stranded (ss) RNA into double-stranded (ds) RNA. By contrast, it has been proposed that RNA silencing induced by self-complementary hairpin RNA (hpRNA) does not require RDR6, because the hpRNA can directly fold back on itself to form dsRNA. However, it is unclear whether RDR6 plays a role in hpRNA-induced RNA silencing by amplifying dsRNA to spread RNA silencing within the plant. To address the efficiency of hpRNA-induced RNA silencing in the presence or absence of RDR6, Wild type (WT, Col-0) and rdr6-11 Arabidopsis thaliana lines expressing green fluorescent protein (GFP) were generated and transformed with a GFP-RNA interference (RNAi) construct. Whereas most GFP-RNAi-transformed WT lines exhibited almost complete silencing of GFP expression in the T1 generation, various levels of GFP expression remained among the GFP-RNAi-transformed rdr6-11 lines. Homozygous expression of GFP-RNAi in the T3 generation was not sufficient to induce complete GFP silencing in several rdr6-11 lines. Our results indicate that RDR6 is required for efficient hpRNA-induced RNA silencing in plants.
Jae Yong Yoo,이균오,Ki Seong Ko,이상열 한국식물생명공학회 2014 Plant biotechnology reports Vol.8 No.5
Plants and plant cells are emerging as promisingalternatives for biopharmaceutical production withimproved safety and efficiency. Plant cells are capable ofperforming post-translational modifications (PTMs) similarto those of mammalian cells and are safer than mammaliancells with regard to contamination by infectious pathogens,including animal viruses. However, a major obstacle toproducing biopharmaceuticals in plants lies in the fact thatplant-derived N-glycans include plant-specific sugar residuessuch as b1,2-xylose and a1,3-fucose attached to apentasaccharide core (Man3GlcNAc2) as well as b1,3-galactoseand a1,4-fucose involved in Lewis a (Lea) epitopeformation that can evoke allergic responses in the humanbody. In addition, sugar residues such as a1,6-fucose, b1,4-galactose and a2,6-sialic acid, which are thought to playimportant roles in the activity, transport, delivery and halflifeof biopharmaceuticals are absent among the N-glycansnaturally found in plants. In order to take advantage ofplant cells as a system in which to produce biopharmaceuticalsdevelopment of plants producing N-glycanstructures compatible with biopharmaceuticals is necessary. In this article we summarize the current state ofbiopharmaceutical production using plants as well as whatis known about N-glycosylation processes occurring in theendoplasmic reticulum and Golgi apparatus in plants. Finally, we propose and discuss a strategy for and theassociated technical barriers of producing customizedN-glycans via removal of enzyme genes that add plantspecificsugar residues and introducing enzyme genes thatadd sugar residues absent in plants.
Involvement of rice Polycomb protein OsFIE2 in plant growth and seed size
나종국,서미혜,윤인순,이균오,이연희,김둘이 한국식물생명공학회 2012 Plant biotechnology reports Vol.6 No.4
Seed development is a complex but orchestrated process that requires the fine-tuning of parentally governed gene expression, which is regulated by Polycomb proteins. Over the last decade, various Polycomb proteins have been identified and functionally characterized in plants, and it has been found that they form the PRC2suppressor complex, which is involved in various developmental programs, including seed development. In this study, the function of the rice fertilization-independent endosperm gene OsFIE2, which expresses a protein homologous to the Arabidopsis Polycomb protein FIE, was characterized. We also characterized OsEZ1/OsiEZ1,another key component of the PRC2 complex. Both the OsFIE2 and OsEZ11 genes are strongly expressed in leaf and stem compared to other tissues, including root, anther,ovary, and ovule. We further examined whether OsFIE2interacted with OsEZ1 using a yeast two-hybrid system. Interaction analysis showed that OsFIE2 interacted with OsEZ1 but not with Arabidopsis MEA protein. To examine the physiological roles of OsFIE2, 35S:OsFIE2 Arabidopsis lines were generated. Transgenic plants with 35S:OsFIE2 grew faster than wild-type plants during early development. Importantly, they produced bigger seed than the wild type, indicating that OsFIE2 may play an important role in seed size. In addition, we generated pOs-FIE2:GUS plants to examine the spatial expression pattern of OsFIE2. GUS expression was detected in cotyledon but not in any other tissues, suggesting that OsFIE2 expression may be required to suppress homeotic genes in cotyledon.
박성철,정영준,정지현,김일룡,이용재,손효석,강승학,장미경,이균오,이상열,이중로 한국생물공학회 2018 Biotechnology and Bioprocess Engineering Vol.23 No.3
Although subcellular localization and substrate specificity of thioredoxin isoforms have been characterized, there is little information on the specific functions of mtype plant thioredoxins or their interaction targets. Here, we describe the functional characterization of an Oryza sativa thioredoxin m (OsTrxm). We undertook yeast twohybrid screening using OsTrxm as a bait and found three interaction proteins, Pex14 and two Pex5 variants. Furthermore, two cysteines of OsTrxm were sufficient for the interaction between OsTrxm and these peroxisome proteins. To verify whether OsTrxm and the target proteins can be co-localized in vivo, we examined subcellular localization of OsTrxm-GFP and a peroxisomal marker RFP-SKL in Arabidopsis protoplast cells. Surprisingly, we detected OsTrxm localization in the cytosol and chloroplast. We confirmed these results by 2-D PAGE and Western blot analysis. Our results indicate that OsTrxm may play important roles in the cytoplasm for peroxisome biogenesis as well as in redox regulation of chloroplast proteins.
Cell cycle arrest mediated by WEE1 is involved in the unfolded protein response in plants
고기성,유재용,Nirmal Kumar Ramasamy,RIKNOHARMOKO,Bích Ngọc Thị Vũ,박지예,이균오 한국식물생명공학회 2018 Plant biotechnology reports Vol.12 No.5
Activation of the unfolded protein response (UPR) in mammalian cells leads to cell cycle arrest at the G1 phase (Thomas et al., J Biol Chem 288:7606–7617, 2013). However, how UPR signaling affects cell cycle arrest remains largely unknown in plants. Here, we examined UPR and endoreduplication in Col-0, wee1, and ER stress sensing-deficient ire1a&b plants during DNA replication and ER stress conditions. We found that WEE1, an essential negative regulator of the cell cycle, is involved in the maintenance of ER homeostasis during genotoxic stress and the ER stress hypersensitivity of ire1a&b is alleviated by loss-of-function mutation in WEE1. WEE1-mediated cell cycle arrest was required for IRE1–bZIP60 pathway activation during ER stress. In contrast, loss-of-function mutation in WEE1 caused increased expression of UPR-related genes during DNA replication stress. WEE1 and IRE1 were required for endoreduplication during DNA replication stress and ER stress, respectively. Taken together, these findings suggest that cell cycle regulation is associated with UPR activation in different manners during ER stress and DNA replication stress in Arabidopsis.