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($Interferon-{\gamma}$)가 마우스 조골세포의 생물학적 활성에 미치는 영향에 관한 연구
이관훈,김정근,정진형,Lee, Kwan-Hoon,Kim, Jung-Geun,Chung, Chin-Hyung 대한치주과학회 1996 Journal of Periodontal & Implant Science Vol.26 No.1
Interferon(IFN) is a sort of glycoproteins that are produced by activated lymphocyte, monocyte and fibroblast. IFN has anti-viral effects, immuno-defensive mechanism and regulating properties to the several kinds of cells that includes affect on the bone formation and resorption. The effect of IFN on the osteoclast & other tissue cells has been studied in a number of researchers with the limited reports on the osteoblast. The purpose of this study was to evaluate the effects of IFN on the osteoblastic function. The MC3T3/El cell(Mouse osteoblast) was incubated in ${\alpha}-minimum$ essential medium containing 10% FBS. To detect the cytotoxic effect of $IFN-{\gamma}$ on osteoblast, the cells were cultured in 96-well plate to which $IFN-{\gamma}$ of various concentrations were added for 2 days. After staining with trypan blue, total cells and living cells were counted under microscope. To determine the activity of alkaline phosphataset(ALP), various concentrations of $IFN-{\gamma}$ were treated to culture medium, and biochemical assay was performed. $IFN-{\gamma}$ and $IFN-{\gamma}$ plus cycloheximide were added to culture medium separately and then ALP activity were determined. To detect the effect of the $IFN-{\gamma}$ on the bone formation of osteoblast, long-term culture was performed, and calcified nodule formation were observed using von Kossa's staining. After the addition of $IFN-{\gamma}$ with various concentrations to the medium, no cytotoxic effect of $IFN-{\gamma}$ was detected at any concentration. The significant increase in ALP activity of osteoblast were found the concentration of $IFN-{\gamma}$ 500-2500U/ml and the culture time of 24-48 hours respectively. The enhancement of ALP activity by $IFN-{\gamma}$ of osteoblast was decreased significantly by the treatment of cycloheximide. After long-term culture of osteoblast, the nodule formation was found to be increased in number and density by the addition of 500 U/ml $IFN-{\gamma}$. These results suggest that $IFN-{\gamma}$ was affected on the bone formation of osteoblast. Forthemore this kind of study or $IFN-{\gamma}$ to osteoblast will be held continuously.
전장제품의 217-Plus 신뢰도 예측 모형에 대한 연구
이관훈,김철희,정태희 한국신뢰성학회 2014 한국신뢰성학회 학술대회논문집 Vol.2014 No.5
본 연구에서는 신뢰도 예측에 가장 많이 사용되고 있는 MIL-HDBK-217과, 최신 버전인 RIAC 217-Plus<SUP>TM</SUP>(이하 217-Plus라 한다) 예측 모형을 비교하고 각 모형의 특징을 분석하였다. 일반적인 217-Plus 고장률 예측은 “부품신뢰도모형”, “시스템신뢰도모형”에 사용자가 축적한 “경험적데이터” 를 추가할 수 있다. 본 연구에서는 217-Plus 고장률 예측 모형에 ”경험적데이터”로 전장품의 내구시험(HALT) 결과를 바당으로 내구성팩터(II HL )를 추가하여 신뢰도 예측 정도를 향상하는 모형을 제안한다.
군 환경시험규격 현실화를 위한 한반도 자연환경 조건분석
이관훈,송병석,홍원식,강보철,염대원,이도희,김준원,금종주 한국군사과학기술학회 2001 한국군사과학기술학회지 Vol.4 No.2
The required standards for environmental tests for communication and electronic equipment used currently in the army are not standards into which the Korean climate and use environment are taken into consideration. But the environmental testing standards of the U.S. army at the time of its establishment(MIL-STD-810) “A” version has been used without taking into consideration the Korean domestic situation and conditions by simply translating the US standards. Adherence to these standards may limit the ability to supply materials in case of emergency since the increase in the manufacturing costs according to excessively strict standards induces an increase in the acquisition cost of military equipment, unnecessarily high maintenance and repair expenses are consumed, and stocks of parts for the military use have to be retained at all times.
이관훈,김철희,박현우 한국신뢰성학회 2015 한국신뢰성학회 학술대회논문집 Vol.2015 No.5
본 연구는 최근 산업의 발전과 함께 제품 개발 기간의 단축 등으로 산업체에서 개발 제품의 강건성을 단기간에 시험하기 위한 초가속수명시험(HALT) 기법에 대해서 연구하였다. HALT(Highly Accelerated Life Test)시험 기법은 주로 시스템에서 시험되고 있으나, 최근에는 소재(Copper)의 일렉트로마이그레이션(Electromigration) 수명평가 및 적층세라믹콘텐서(MLCC) 등의 신회성평가 등에도 이용되고 있다. 본 연구를 통하여 현재 국내에서 많이 사용되고 있는 초가속수명시험(HALT)의 최근 DATA 161건을 분석하여 설계 마진을 분석하였다. 또한 본 연구에서 제안하는 HALT 설계 마진을 선행 사례와 비교하여 설계 마진 설정의 객관성을 상대 비교하였다.