대하(帶下)의 침구치료(鍼灸治療)에 관(關)한 고찰(考察)

윤현민,Youn, Hyoun-min 대한침구의학회 2004 대한침구의학회지 Vol.21 No.2

Objective : To review was to study acupuncture and moxibustion treatments for abnormal leukorrhea. Normal leukorrhea is always secreted in female vagina. It increased usually by infection (Trichomoniasis and Candidiasis), turmor and so on. Methods : We extracted the parts and acupuncture and moxibustion leukorrhea from ancient and modern oriental medical literature. We have got compared and analyzed 45 kinds of books since Nei Jing(內經). Results and Conclusion : In acupuncture therapy meridians of Bladder, Stomach, Spleen and Conception vessel were mostly used for them in connection with the functions of each meridians. The acupoint used on acupunture only were 78 acupoints. In particular, Chung-guk(cv3), Kwan-won(cv4) point of Conception vessel Meridian and Samum-gyo point of Spleen meridian are frequently applied in leukorrhea.

Effect of Juglans sinensis Dode extract on chemical hypoxia-induced cell injury in human glioma cells

윤현민,허재영,안창범,Youn, Hyoun-min,Heo, Jae-yeong,Ahn, Chang-beohm The Korean AcupunctureMoxibustion Medicine Society 2003 대한침구의학회지 Vol.20 No.2

이 논문(論文)은 활성 산소(ROS)의 작용(作用)을 규명하고 호도약침액(胡桃藥鍼液)이 인간의 신경교종 세포인 A172에서 화학적(化學的) 저산소증(低酸素症)으로 유발된 세포 사멸에 대해 효능이 있는지를 연구(硏究)한 것이다. 화학적(化學的) 저산소증(低酸素症)은 세포내 미토콘드리아의 전자 수송을 방해하는 antimycin A를 가진 배양세포에 의해 유발(誘發)하였다. 화학적(化學的) 저산소증(低酸素症)에 노출된 세포(細胞)는 시간과 그 양에 따라서 세포 사멸의 결과(結果)가 다르게 나타난다. 화학적 저산소증에 의해서 ROS의 생산이 증가하는데 이것은 $H_2O_2$ 소거(消去) Catalase(과산화수소를 물과 산소로 분해하는 효소)에 의해 방지(防止)된다. Catalase는 화학적 저산소증에 의해 유발(誘發)된 세포 사멸을 방지하는데 비해 DMTU는 효과적이지 않다. 지질(脂質)에 녹는 산화방지제 DPPD와 물에 녹는 산화방지제 Trolox는 세포사멸을 방지하는데 효과(效果)가 없다. 호도약침액(胡桃藥鍼液)은 그 양(量)에 의존적으로 저산소증에 의해 유발된 세포 사멸을 방지하는 효과가 있다. 즉 화학적 저산소증으로 유도된 ROS의 발생을 막고, $H_2O_2$로 유도된 세포사멸을 방지하는데 이것은 화학적 저산소증과 $H_2O_2$의해 유도된 세포사멸에 대해 호도약침액(胡桃藥鍼液)이 방지효과(防止效果)가 있다는 것을 의미한다. 이러한 결과(結果)들은 $H_2O_2$가 지질 과산화와는 무관한 메카니즘으로 저산소증(低酸素症)으로 유발(誘發)된 세포사멸을 중재하고, 따라서 호도약침액(胡桃藥鍼液)은 지질막의 과산화를 방지하기 보다는 ROS를 직접적으로 소거(消去)함으로써 방지 효과가 있다는 것을 의미한다. 더구나 화학적(化學的) 저산소증(低酸素症)은 caspase와 무관한 메카니즘으로 apoptosis를 유발(誘發)한다.

뇌졸중(腦卒中) 환자(患者) 형태(形態)에 관(關)한 임상연구(臨床硏究)

윤현민,안창범,송춘호,손인석,장경전,Youn, Hyoun-min,Ahn, Chang-beohm,Song, Choon-ho,Son, In-seok,Jang, Kyung-jeon 대한침구의학회 2003 대한침구의학회지 Vol.20 No.2

Clinical observation was made on 52 cases of Stroke that were confined through brain CT, MRI scan. The Stroke cases wee classified into the following kinds cerebral infarction, cerebral hemorrhage, cerebellar or brain stem infarction, cerebellar or brain stem hemorrhage. And among the 52 cases of Stroke cerebral infarction was noticed in 75.00%, cerebral hemorrhage in 11.54%, cerebellar or brain stem infarction in 9.52%, cerebellar or brain stem hemorrhage in 3.85%. The ratio between males and females was 1.74:1 in the whole groups of Stroke and most cases were over 60 of age. As the time of hospitalization, most patients hospitalized from 1 day after stroke to 7 days after stroke. And as the course of hospitalization, most patients hospitalized first. Among the preceding disease at the onset of Stroke hypertention was noted in 32.69%, and deabetes mellitus or heart problem was noted frequently(15.39%). Electrocardiography findings were as follows: The normal was noted in 53.85%, the abnormal in 46.15%. And as the abnormal, left ventricular hypertrophy was noted in 17.54%. The predisposing factors or conditions at the onset of brain infarction were usually initiated during the time of sleeping and those of brain hemorrhage chiefly during the time of exercising like overwork or walking etc. It was noted that smoking a pack of cigarette showed highest disease rate(33.33%) among the average of smoking amount of one day in case of man. Prior to attack, the most chiefly complain was dyspnea or discomfort on chest region. And 30.70% of patients had no previous sign. There were a large number of recurrent cases. The first attack was noted in 71.15%, the 2nd attack in 23.08%, the 3rd attack in 5.77%.

구중질환(口中疾患)의 침구치료(鍼灸治療)에 관한 고찰(考察)

윤현민,안창범,김철홍,Youn, Hyoun-Min,Ahn, Chang-Bum,Kim, Cheol-Hong 경락경혈학회 2004 대한침구의학회지 Vol.21 No.1

Objectives : To study acupuncture and moxibution treatments for stomatopathy( aphthae, oral ulceration, mycolic stomatitis, halitosis, thirst, bitter) the ancient and the present literatures were reviewed. Methods : We've got compared and analyzed 55 kinds of literatures. Results and Conclusions : 1. The acupuncture meridians used frequently for stomatopathy were $Su-yangmy\bar{o}ng-Taejang-ky\bar{o}ng(LI),\;Chok-yangmy\bar{o}ng-Wi-Ky\bar{o}ng(S),\;Immaek-Ky\bar{o}ng(CV)$. 2. The acupoints used frequently for aphthae were $Sungjang(CV_{24}),\;Yomchon(CV_{23}),\;Hapkok(LI_4),\;Nogung(P_8),\;Chok-samni(S_{36}),\;Hyopko(S_6),\;Sugu(GV_{26})$. 3. The acupoints used frequently for oral ulceration were $Hapkok(LI_4),\;Nogung(P_8),\;Chok-samni(S_{36}),\;Kokchi(LI_{11}),\;Sotaek(SI_1),\;Pisu(B_{20}),\;Wisu(B_{21}),\;Samgan(LI_3),\;Yomchon(CV_{23}),\;Chichang(S_4)$. 4. The acupoints used frequently for mycolic stomatitis were $Hapkok(L_4),\;Chichang(S_4),\;Hyopko\;(LI_4),\;Sungjang(CV_{24}),\;Samumgyo(SP_6)$. 5. The acupoints used frequently for halitosis were $Naejong(S_{44}),\;Chok-samni(S_{36}),\;Chung-wan\;(CV_{12}),\;Sang-wan(CV_{13}),\;Hawan(CV_{10}),\;Kongson(SP_4),\;Wisu(B_{21}),\;Nogung(P_8),\;Sugu(GV_{26}),\;Sungjang(CV_{24})$. 6. The acupoints used frequently for thirst were $Sosang(L_{11}),\;Sangyang(LI_1),\;Sotaek(SI_1),\;Kwanch'ung(TE_1),\;Ch'\bar{o}kt'aek(L_5),\;T'ae-gye(K_3),\;Kokt'aek(P_3),\;Sugu(GV_{26}),\;Samgan(LI_3),\;Igan(LI_2),\;T'aech'ung(Liv_3),\;Sojangsu(B_{27})$. 7. The acupoints used frequently for bitter were $Yangn\bar{u}ngch'\bar{o}n(G_{34}),\;Hy\bar{o}njong(G_{39}),\;Kwanch'ung(TE_1),\;Tamsu(B_{19}),\;Chokkyu\bar{u}m(G_{44}),\;Y\bar{o}n-gok(K_2),\;Shinmun(H_7),\;Chok-Samni(S_{36})$.

족삼리자침(足三里刺鍼)이 가토소장(家兎小腸)의 운동성(運動性)에 미치는 영향(影響)

이방성,윤현민,장경전,송춘호,안창범,Lee, Bang-Sung,Youn, Hyoun-Min,Jang, Kyung-Jeon,Song, Choon-Ho,Ahn, Chang-Beohm 대한침구의학회 2000 대한침구의학회지 Vol.17 No.2

This study was undertaken to determine effect of Zusan1i(ST36) acupuncture (AP) on the small intestinal motility in the rabbit. Changes in the motility in vivo of ileum was measured before and 20 min. after AP. In order to examine whether AP effect was affected by autonomic nerves, norepinephrine or acetylcholine was applicated. AP significantly increased the mean wave amplitude and motor index, but not the mean wave duration and motor activity. Infusion of norepinephrine ($2{\mu}m$) into an ear vein resulted in a decrease in the mean wave amplitude and motor index, which was not affected by AP. Acetylcholine ($10{\mu}m$) produced inhibition followed by stimulation of motility. The stimulation of motility was induced by an increase in the mean wave amplitude and motor index, but not the mean wave duration and motor activity. The effect of acetylcholine was not significantly different between before and after AP. These results suggest that Zusan1i(ST36) acupuncture stimulates the small intestinal motility, which is not mediated by autonomic nerves. Therefore, Zusan1i(ST36) acupuncture seems to be applicable to impaired small intestinal movement.

원저 : 호도약침이 인간의 신경교종 세포에 유발된 저산소증에 대한 방어효과

윤현민 ( Hyoun Min Youn ),허재영 ( Jae Yeong Heo ),안창범 ( Chang Beohm Ahn ) 대한침구학회 2003 대한침구의학회지 Vol.20 No.2

이 論文은 활성 산소(ROS)의 作用을 규명하고 胡桃藥鍼液이 인간의 신경교종 세포인 A172에서 化學的 低酸素症으로 유발된 세포 사멸에 대해 효능이 있는지를 硏究한 것이다. 化學的 低酸素症은 세포내 미토콘드리아의 전자 수송을 방해하는 antimycin A를 가진 배양세포에 의해 誘發하였다. 化學的 低酸素症에 노출된 細胞는 시간과 그 양에 따라서 세포 사멸의 結果가 다르게 나타난다. 화학적 저산소증에 의해서 ROS의 생산이 증가하는데 이것은 H_2 O_2 消去 Catalase(과산화수소를 물과 산소로 분해하는 효소)에 의해 防止된다. Catalase는 화학적 저산소증에 의해 誘發된 세포 사명을 방지하는데 비해 DMTU는 효과적이지 않다. 脂質에 녹는 산화방지제 DPPD와 물에 녹는 산화방지제 Trolox는 세포사멸을 방지하는데 效果가 없다. 胡桃藥鍼液은 그 量에 의존적으로 저산소증에 의해 유발된 세포 사멸을 방지하는 효과가 있다. 즉 화학적 저산소증으로 유도된 ROS의 발생을 막고, H_2 O_2로 유도된 세포사멸을 방지하는데 이것은 화학적 저산소증과 H_2 O_2의해 유도된 세포사멸에 대해 胡桃藥鍼液이 防止效果가 있다는 것을 의미한다. 이러한 結果들은 H_2 O_2가 지질 과산화와는 무관한 메카니즘으로 低酸素症으로 誘發된 세포사멸을 중재하고, 따라서 胡桃藥鍼液은 지질막의 과산화를 방지하기 보다는 ROS를 직접적으로 消去함으로써 방지 효과가 있다는 것을 의미한다. 더구나 化學的 低酸素症은 caspase와 무관한 메카니즘으로 apoptosis를 誘發한다.

사상체질의 혈액학적 분석에 따른 고찰

윤현민(Hyoun-Min Youn),이채우(Chae-Woo Lee),서현숙(Hyoun-Sook Seo) 한의병리학회 2005 동의생리병리학회지 Vol.19 No.3

견정(肩井), 곡지(曲池), 족삼리(足三里) 시침(시鍼)이 뇌졸중(腦卒中) 환자(患者)의 혈압(血壓)과 두통(頭痛)에 미치는 효과

윤현민 ( Hyoun Min Youn ),강귀염 ( Kwi Yeum Kang ),이지원 ( Ji Won Lee ) 대한경락경혈학회 2005 Korean Journal of Acupuncture Vol.22 No.3

Objective: The purpose of this study was to verify the effect of pressing at Kyeun-Jung(G21), Gok-Ji(LI11) and Zok-Sam-Li(S36) with Si-acupuncture on the decreasing of blood pressure and headache on the stroke patients. Methods: This study was one-group pretest-posttest experimental design. The subjects of this study were 30 patients who were hospitalized in stroke center of hospital located in Ulsan from September 27, to November 12, 2004. They had over 160/90mmHg blood pressure but didn`t take any hypotensive drugs. The instruments were the Si-acupuncture, the blood pressure and headache scale using Visual Analogue Scale(VAS). Results: 1. With administration of Si-acupuncture, the blood pressure intensity decreased statistical significantly(systolic blood pressure: t=13.310, p=0.000, diastolic blood pressure: t=11.788, p=0.000). After 30 minutes with administration of Si-acupuncture, the blood pressure intensity decreased statistical significantly(systolic blood pressure: t=15.130, p=0.000, diastolic blood pressure: t=12.479, p=0.000). 2. With administration of Si-acupuncture, the headache intensity not decreased statistical significantly"(t=0.372, p=0.712). After 30 minutes with administration of Si-acupuncture, the headache intensity decreased statistical significantly"(t=7.370, p=0.000). Conclusion: According to above, it was verified that the pressing at Kyeun-Jung, Gok-Ji and Zok-Sam-Li with Si-acupuncture was effective in decreasing of blood pressure and headache on the stroke patients.

호도약침액(胡桃藥鍼液)의 유리기(遊離基) 소거(消去)와 항산화(抗酸化) 효과(效果)에 대한 실험적(實驗的) 연구(硏究)

김철홍,윤현민,장경전,송춘호,안창범,Kim, Cheol-hong,Youn, Hyoun-min,Jang, Kyung-jeon,Song, Choon-ho,Ahn, Chang-bum 대한침구의학회 2003 대한침구의학회지 Vol.20 No.4

This study was performed to determine if Juglandis semen extract(JSE) has free radical scavenging and antioxidant activities. Superoxide anion generation by xanthine oxidase/xanthine and in neutrophils activated by phorbol-12, 13-dibutyrate was inhibited by JSE and its effect was dose-dependent. JSE also inhibited generation of $H_2O_2$ induced by glucose oxidase/glucose and in opossum kidney cells treated with antimycin A. JSE exerted a direct $H_2O_2$ scavenging effect. Exposure of opossum kidney cells to 1mM tBHP caused a significant increase in lipid peroxidation, which was prevented by JSE. JSE also prevented tBHP-induced LDH release. These data suggest that JSE has free radical scavenging and antioxidant activities. However, further studies should be carried out to find the active ingredient(s) of JSE that exerts radical scavenging action.

영지(靈芝) 약침(藥鍼)과 영지(靈芝) 추출액의 항알레르기 효과에 대한 연구

강경화,윤현민,Kang, Kyung-Hwa,Youn, Hyoun-Min 대한약침학회 2007 Journal of pharmacopuncture Vol.10 No.3

Objectives We studied on anti-allergic effects of Ganoderma lucidum herbal acupuncture(GHA) and Ganoderma lucidum extract(GE). Methods In vivo, Animals were herbal-acupunctured GHA at both B13s three times for 5 days. Then, we investigated compound 48/80-induced active systemic anaphylatic shock using ICR mice and anti-DNP IgE-induced passive cutaneous anaphylaxis using Sprague Dawley rat. In vitro, we measured cell viability, b-hexosaminidase release, IL-4 and TNF-a from RBL-2H3 cells, and nitric oxide from Raw264.7 cell after treatment of GE of various concentrations. Results In vivo, GHA pretreatments at both B13s inhibited compound 48/80-induced active systemic anaphylatic shock. Passive cutaneous anaphylaxis were inhibited by GHA10 and OP. In vitro, $0.1\;{\sim}\;2%$ GE treatments were not affect on cell viability and inhibited b-hexosaminidase release, IL-4, TNF-a and nitric oxide. Conclusions These results suggest that GHA and GE may be beneficial in the inhibition of allergic inflammatory response.