인구 분포에 따른 방사선 장비 및 종사자에 관한 고찰

윤철호,최준구,Yoon, Chul-Ho,Choi, Jun-Gu 대한방사선치료학회 2009 대한방사선치료학회지 Vol.21 No.2

Purpose: The purpose of this paper is to provide basic data in order to systemize the management of demand and supply of radiologic technicians, to pursue a fair regional distribution of educational institutions, and furthermore to keep reasonable medical treatment and fee. This research was carried out through the investigation of radiologic equipments and technicians according to the distribution of the population. Materials and Methods: We compared and analyzed the correlation between regional population, the number of clinics and hospitals, the number of medical imaging devices, and the number of radiologists and radiologic technicians in 5 cities without "Gu" administrative units in 2008. Results: 27,317 radiologic technicians have been produced since the administration of the national qualifying exam for radiologic technicians. About 18,000 radiologic technicians are currently working. There are 39 colleges or universities with Departments of Radiology and the admission quota is 2,120 students excluding one college. The ratio of radiologic equipments to radiologic technicians is 2.6 to 1. Conclusion: There is a dilemma in which some radiologic technicians fail to find appropriate jobs while some clinics or hospitals are in need of radiologic technicians. This dilemma is due to unreasonable regional discrepancies in pay system and welfare situation, and excessive profit-oriented recruiting system of clinics and hospitals. The increase of students of Radiologic Departments and approval of additional departments will end up with producing superfluous high academic degree holders, which is on the contrary to the government policy to produce more job opportunities. So the policy of increasing Radiologic Departments should be reconsidered.

6 MeV LINAC 방사선 조사에 의한 생쥐 난소조직의 미세구조 변화

윤철호,문명진,Yoon, Chul-Ho,Moon, Myung-Jin 한국현미경학회 2003 Applied microscopy Vol.33 No.2

본 연구는 6 MeV LINAC에서 발생한 X-선을 생쥐 생체에 조사한 후, 방사선 조사선량에 따른 난소 조직의 미세구조적 변화를 광학 및 전자현미경으로 관찰하였다. X-선을 200 cGy의 선량을 조사한 후 적출한 난소조직에서 성장난포의 과립층세포는 핵이 응축되어 나타났고, 난자를 둘러싸고 있는 투명층은 정상 대조군에서보다 경계가 불규칙하게 관찰되었다. 400 cGy로 조사된 난소조직에서 과립층세포는 대부분 응축된 핵을 가지고 있었으며, 피폭된 과립층세포의 사멸에 의한 세포질의 단백질 변성이 확인되었다. 600 cGy의 선량을 조사한 난소의 조직표본에서 성장난포는 위축되어 있었으며, 난포동에는 백혈구가 침윤되어 있었고, 난포액은 정상난포와 달리 불균질한 물질로 채워져 있었다. 투과전자현미경으로 관찰한 결과, 200 cGy의 방사선을 조사한 성장난포에서 과립층세포는 정상난포와는 달리 불규칙한 모양을 하고 있었다. 과립세포 사이의 난포동에는 많은 세포성분의 부스러기가 산재하여 있었으며, 이들 주변에 호중구와 대식세포가 관찰되어, 이 시기에 이미 방사선 피폭에 의한 세포사의 기작이 진행되고 있음이 확인되었다. X-선을 600 cGy로 조사한 난소조직의 과립층세포는 세포의 형태나 핵의 변형이 현저하여, 일부 세포는 핵의 분절화가 진행되는 등, apoptosis의 전형적인 특징이 관찰되었다. 특히 성장난포의 난포동에서는 염증반응에 관여하는 호중구가 잘 관찰되었으며, 주변에 산재된 세포 파쇄물 및 apoptotic body와 함께 대식세포의 출현도 확인되었다. This research investigates the fine structural as well as the morphological changes of the mouse ovarian tissue after irradiation of various dose rates of 6 MeV LINAC radiation. The normal structure of the ovarian tissue is consisted of various stages of follicles including primordial and growing follicles, and ovarian stromal connectives. When we observed the ovarian tissues irradiated with a dose rate of 200 cGy/min using light and electron microscopes, granular cells in growing follicles are in irregular shape unlike normal follicles. Small segments of cells scattered in follicular antrum among granular cells. We could observe neutrophils and macrophages around the segments, which means the cells already got in the process of decease owing to the effects radiation. With coincident to the increase of the dose rate of x-ray irradiation as 400 or 600 cGy/min, the mature follicles appeared as an irregular form and the granular cells surrounding oocyte also deformed comparing to their normal counterparts. The granulosa cells within mature follicle are already occurred necrotic change and apoptosis. The nuclei in some cells got so fragmented that the segments formed the shape of a horseshoe or scattered in small and condensed pieces. All the cells at a granular layer irradiated with a dose rate of 600 cGy/min show typical characteristics of apoptosis. The neutrophils involved in inflammatory reaction appear evidently in follicular antrum of growing follicles, and macrophage scattered with residual and apoptotic bodies.

방사선 조사선량에 따른 생쥐 난소의 TUNEL 염색반응에 관한 형태학적 연구

윤철호,장병수,Yoon, Chul-Ho,Chang, Byung-Soo 한국현미경학회 2007 Applied microscopy Vol.37 No.2

본 연구는 6 MeV LINAC에서 발생한 X-선을 생쥐 생체에 조사한 후 난소에서 난포의 형태적 변화 양상과 난포의 세포자연사가 일어나는 과정을 TUNEL 염색방법을 이용하여 광학현미경으로 관찰하였다. 정상난포와 퇴화난포 및 방사선이 조사된 난포에서 세포예정사가 발생하는 것을 확인하기 위해서 TUNEL 염색을 실시한 결과, 정상난소의 퇴화난포에서 양성반응을 보이는 과립층세포들은 갈색을 띠고 있었고, 핵은 응축되어 나타났다. 그러나, 정상난포에서는 양성반응이 나타나지 않았다. X-선을 조사한 난소의 난포는 TUNEL 염색에 강한 양성반응이 나타났고, 600 cGy의 X-선 조사에서 난모세포는 이미 세포예정사가 진행되어 파괴되었음을 확인할 수 있었다. 그리고 난포막을 형성하고 있는 난막세포의 핵들도 양성반응으로 나타나, 갈색으로 염색이 되었으며, 수질의 결합조직세포들의 핵도 갈색으로 염색되어 관찰되었다. 또한, 대부분의 세포들은 세포예정사가 진행되어 있으며, apoptotic body들이 난포 내에 산재되어 있었다. 이 시기의 난소조직의 전반적인 염색도는 저선량의 X-선 조사에서보다 더 현저히 강한 염색성이 나타났다. This research investigated morphological changes of ovarian follicle according to dose of irradiation when adult mice were exposed to X-rays from 6 MeV LINAC. At day 3 after irradiation of 200 cGy, 400 cGy and 600 cGy X-ray to the hole body of mice, the ovaries collected and stained with TUNEL. The normal follicles and atretic follicles were identified to apoptosis by the staining with TUNEL. In the atretic follicles of the normal ovary, the apoptotic bodies were well appeared and stained brown color. Almost of the follicles following irradiation are stained with TUNEL, but the sensitivity of reaction is weaker than that in irradiation of 400 cGy and 600 cGy X-ray. The granulosa cells of the radiated normal follicle by 400 cGV are shown brown color. In this stage, the nucleus of granulosa cells in the atrectic follicles are condensed and picknotic feature. The size of the radiated follicle by 600 cGy are decreased than the normal follicles. The atropic follicles are filled with apoptotic bodies which change of granulosa cells and theca cells by influence of X-ray. All of cell in the follicles are strongly positive stained with TUNEL by irradiation of 600 cGy.

전문 방사선사 제도에 관한 설문조사 분석

윤철호,박성옥,Yoon Chul Ho,Park Soung Ock 대한방사선사협회 2001 대한방사선사협회지 Vol.27 No.2

A men who has obtain a license of radiotechnologist from the government engaging in the Dept. of radiology as a technologist at various hospitals. They can engage in various part such as Diagnosis radiology, Radiation therapy, Sonography and Nuclear medic

IL-1${\beta}$ 처리(處理) 당뇨병(糖尿病) 마우스의 췌장(膵臟) glucokinase 및 hexokinase 활성(活性)에 대(對)한 비파엽(枇杷葉)의 영향(影響)

윤철호,신현철,정지천,Yoon, Cheol-Ho,Shin, Hyeon-Chul,Jeong, Ji-Cheon 대한한방내과학회 1998 大韓韓方內科學會誌 Vol.19 No.1

We investigated the in vivo effect of an aqueous extract (referred to as EF) from Eriobotryae folium on glucokinase and hexokinase activities of diabetes mellitus induced by $interleukin-1{\beta}\;(IL-1{\beta})$. After 1 week of $IL-1{\beta}$ injection, the levels of serum glucose concentration and insulin secretion were dramatically increased. However, the insulin secretion was decreased with administration of EF. The level of glucose concentration was decreased by EF administration. Furthermore, it was observed that EF was effective in recovering the levels of insulin secretion. Enzyme activities of the glucokinase and hexokinase, which are key enzymes of glucose phosphorylastion, were decreased by $IL-1{\beta}$. EF administration to the mice allowed proportional increasing by stimulation of induction of enzyme activities as high as normal group. These results suggested that EF is highly effective in treatment of diabetes mellitus induce by $IL-1{\beta}$.

방사선 조사선량에 따른 생쥐 난소의 조직화학적 변화

윤철호(Chul-Ho Yoon) 대한방사선과학회(구 대한방사선기술학회) 2006 방사선기술과학 Vol.29 No.4

  본 연구는 X-선을 생쥐 생체에 조사한 후, 방사선 조사선량에 따른 난소조직의 형태적 변화를 Masson"s trichrome, 세망섬유 염색법 등의 조직화학반응을 확인한 결과 X-선을 조사한 난소조직은 방사선량이 증가함에 따라 성장 난포의 형태적 변화가 뚜렷하였으며, 특히 과립층세포의 변성에 의한 핵은 응축과 투명대의 변화가 현저하였다. 또한 Masson"s trichrome 염색과 세망섬유 염색을 통한 조직화학반응의 결과 과립층세포의 변화와 난포기저막의 변형, 그리고 세망섬유의 비정상적 배열이 확인되었다.   This research investigated the morphological changes of folliculus ovary according to the dose of the X-rays when mice were exposed to X-rays, in terms of histochemical reaction. Dyeing methods such as Masson"s trichrome method and reticular fiber method were used. The morphological changes of growing folliculus ovary got distinct as the dose of X-rays increased. Especially, the nuclei of granular cells showed manifest condensation and the changes of the transparent zone were distinct. As a result of histochemical reaction according to Masson"s trichrome method and reticular fiber method, the changed granular cells, the deformed basilar membrane of folliculus ovary and the abnormal arrangement of the reticular fiber were observed.

방사선 조사선량에 따른 생쥐 난소의 미세구조적 연구

윤철호(Chul Ho Yoon) 대한방사선과학회(구 대한방사선기술학회) 2005 방사선기술과학 Vol.28 No.3

본 연구는 6 MV LINAC에서 발생한 X-선을 생쥐 생체에 조사한 후, 방사선 조사선량에 따른 난소조직의 미세구조적 변화를 고배율의 전자현미경을 이용하여 관찰하였다. 방사선 조사에 따른 난소조직의 미세구조 변화를 관찰하기 위하여 고배율의 전자현미경으로 관찰한 결과, 방사선량의 증가에 따라 성장난포의 과립층세포에서 핵과 세포질의 미세구조 변형이 급격히 증가 하였으며, 난포동에서는 세포사의 부산물인 세포 잔류체들과 백혈구 및 대식세포 등이 관찰되었다. 과립층세포의 미세구조적 변형은 주로 핵의 응축에 의한 전자밀도의 증가와 핵의 분절화, 그리고 세포질의 위축 등, 전형적인 세포예정사의 특성을 나타내고 있었다. 세포의 괴사도 일부 확인되었으나 그다지 현저하지 않았으며, apoptotic body와 함께 대식세포가 산재되어 있었다. This study investigated the structural changes of folliculus ovarii according to the dose of the X-rays when mice were exposed to X-rays from 6MeV LINAC. The minute structural changes of folliculus ovarii were observed through an electron microscope with high magnification. Nuclei and protoplasm of granular cells in growing folliculus ovarii abruptly underwent minute structural changes according to the increase of dose of X-rays. Cell residue, by-product of cell decease, neutrophil and macrophage around follicular antrum were observed. The minute structural changes in granular cells showed typical characteristics of apoptosis: the increase of electronic density due to nuclear condensation, fragmentation of nuclei, and atrophy of protoplasm. Necrosis of cells was identified, but it was not so remarkable. Macrophage scattered with apoptotic bodies.

방사선 종사자 근무 분야별 피폭에 관한 검토

윤철호(Chul-Ho Yoon),윤석환(Seok-Hwan Yoon),최준구(Jun-Gu Choi) 대한방사선과학회(구 대한방사선기술학회) 2008 방사선기술과학 Vol.31 No.3

  본 논문은 2006. 1. 1~12. 31(1년)까지 방사선 종사자 근무분야별 피폭에 관하여 서울시내 3차 의료기관인 A, B, C병원의 방사선 분야 종사자 방사선 피폭선량에 대한 분석 결과, 다음과 같은 결론을 얻었다.<BR>  1. 방사선 종사자 근무분야별 피폭은 심혈관조영실이 1.41 mSv로서 제일 많았고, 방사선종양학과는 0.64 mSv로서 제일 낮았다.<BR>  2. 방사선 종사자 근무분야별 피폭선량은 시술건수에 비례하였다.<BR>  3. 방사선 종사자 근무분야별 시술건수 당 피폭선량은 심혈관조영실 근무자가 많았고, 영상의학과 근무자는 적은 것으로 나타났다.<BR>  4. 방사선 종사자 근무분야별 피폭선량 순위는 심혈관조영실, 핵의학과, 영상의학과, 방사선종양학과의 순이었다.<BR>  이상과 같은 결과로 볼 때, 국제방사선방어위원회(ICRP) 권고안 범위이내이므로 병원 방사선 종사자들의 피폭에 따른 유해는 없는 것으로 판단되었다.   Radiation dose to radiologists working at three hospitals in Seoul was investigated from Jan 1, 2006 to Dec. 31, 2006. The results are as follows.<BR>  First, radiation dose to radiologists at a cardiac angiography room was measured as 1.41 mSv, the highest while radiation dose to radiologists at a department of radiation oncology was measured as 0.64 mSv, the lowest.<BR>  Second, radiation dose proves to be in direct proportion to the number of X-ray treatment.<BR>  Third, as for the radiation dose in X-ray treatments, radiologists in cardiac angiography room are exposed to the largest amount of radiation while radiologists in diagnostic radiology department are exposed to the smallest amount of radiation.<BR>  Last, radiation dose at a cardiac angiography room is the largest and is followed by nuclear medicine, diagnostic radiology, and radiation oncology departments in order.<BR>  According to ICRP, exposure less than 20 mSv per year is highly recommended while radiation dose is allowed as long as it is ranged less than 50 mSv per year or 100 mSv within a 5-year period. Taking into account the results, radiation exposure does not do any harm to radiologists at any related departments in Korean hospitals because the dose per year is less than 1.60 mSv.

정부 전산망에서 데이타베이스 시스템 활용을 위한 제언

윤철호(Chul-Ho Yoon) 한국정보과학회 1991 한국정보과학회 워크샵 Vol.1991 No.3

고체상 추출 카트리지와 유도결합 플라즈마 질량분석기를 이용한 자연수내 무기 비소의 종분화 분석

윤철호 ( Cheol Ho Yoon ),윤혜온 ( Hyeon Yoon ),박용철 ( Yong Chul Park ),박찬수 ( Chan Soo Park ),홍종기 ( Jong Ki Hong ) 한국환경분석학회 2003 환경분석과 독성보건 Vol.6 No.3