산수유, 보골지 복합추출물의 Sprague-Dawley rat를 이용한단회경구투여 독성시험

진보람,강현애,Divina C. Cominguez,김명석,이광호,윤일주,안효진 대한본초학회 2019 大韓本草學會誌 Vol.34 No.3

Objectives : In this animal study, we performed the single oral dose toxicity test of Standardized Cornus officinalis Sieb. et Zucc. and Psoralea corylifolia L. 30% ethanol extract (SCP) in Sprague-Dawley (SD) rats owing to aims for verifying approximate lethal dose (ALD). Methods : According to OECD guidelines for the testing of chemicals section 4 health effects test No. 420 acute oral toxicity study – fixed dose procedure (17 December 2001), single oral dose toxicity test was performed. Animals were divided into two groups: Group 1, vehicle-treated rats (Control); Group 2, SCP 5000 ㎎/㎏ treated rats. SCP is composed of two medicinal herbs: Cornus officinalis Sieb. et Zucc. (650 g) and Psoralea corylifolia L. (350 g) in 30% ethanol. SCP was once orally administered to female and male SD rats at dose levels of 5000 ㎎/㎏. Animals were monitored on the mortality, clinical signs, body weight changes and necropsy findings for 14 days. Results : After single oral treatment of SCP, we could not find any mortality up to 5000 ㎎/㎏. Compared with the control group, there were also no significant differences in clinical sign, weight change, weight gain and gross abnormalities in SCP 5000 ㎎/㎏-treated group. Conclusions : Taken together, these results suggest that the ALD of SCP in both female and male SD rats were considered as over 5000 ㎎/㎏. Results from this study provide scientific evidence for the safety of SCP.

HBX-6의 Sprague-Dawley rat를 이용한 단회경구투여 독성시험

진보람,서동욱,김명석,이광호,윤일주,김창은,안효진 대한본초학회 2018 大韓本草學會誌 Vol.33 No.1

Objectives : This study was performed to investigate the single oral toxicity of HBX-6 in Sprague-Dawley (SD) rats. Methods : Twenty SD rats were randomly assigned to four groups of 5 rats each and were administrated singly to female and male SD rats, as an oral dose of 2000 ㎎/㎏. HBX-6 is a newly combined Korean herbal medicine formula 30 % Ethanol extract derived from The Dongui Bogam. Now we are developing the prescription for the aim of improving benign prostatic hyperplasia (BPH) without undesirable side effects. HBX-6 is composed of nine medicinal herbs: Aconiti Lateralis Radix Preparata, Corni Fructus, Cistanchis Herba, Psoraleae Semen, Dendrobii Herba, Morindae Radix, Cuscutae Semen, Trigonellae Semen, Foeniculi Fructus. Animals were monitored for the mortality and changes in the body weight, clinical signs, gross observation and necropsy findings for the 14 days according to “Standard for Toxicity Study of Pharmaceuticals” of Korea Food and Drug Administration (KFDA) guideline and “Acute Oral Toxicity – Fixed Dose Procedure” of OECD Test Guideline. Results : We could not find any mortality. Compared with the control group, significant weight change was not observed in the experimental group. After administration, the more common symptoms were not observed. There were no gross abnormalities in all cases. Conclusions : Taken together, these results suggest that the approximate lethal dose of HBX-6 in both female and male SD rats were considered as over 2000 ㎎/㎏.

地區單位計劃의 物理的 規制要素가 街路景觀에 미치는 影響에 관한 硏究

정성구,최민후,윤진보,신남수 대한건축학회 2004 대한건축학회논문집 Vol.20 No.10

Emphasis has recently been laid on the importance of street space that constitutes urban space in stimulating and strengthening the functions of the downtown area In most urban areas, however, street space has deteriorated so much that it reveals barrenness This situation is owing not to the lack of proper understanding about the importance of street space planning, but to the paucity of accumulated study on the correlation between the spatial consciousness of man as the subject of a street space evaluation on the one hand and the constituent elements as the object of spatial formation on the other District unit planning is a social system that is concerned with developing street space It maps out ideal urban space for better urban environment It also aims to better utilize urban space in accordance with the size and complexity of a city The purpose of this thesis is to examine the correlation between urban planning control elements regulating constituent elements of street space and the structure of man's psychological evaluation of the streetscape It explores the influence of physical regulatory elements on the streetscape, thus providing basic data and guidelines for planning a better streetscape It makes a psychological analysis of different applications of controlling elements by employing a semantic differential method and a CG simulation The procedure of my research is first to choose adjectival pairs that describe street space through bibliographical study and preliminary survey, and then to select physical regulatory elements concerning district Unit planning My study also shows the influence of the design control elements of distinct unit planning on the streetscape, by experimenting with those elements by means of CG simulation It examines variables on the axis of psychological factors according to the variation of regulatory elements and the degree of the influence of those variables

主要 眺望點에서 바라본 港灣景觀의 注視特性에 관한 硏究

김은일,정성구,윤진보,신남수 대한건축학회 2004 대한건축학회논문집 Vol.20 No.7

It is necessary to research for environmental features of regions rather than each building and scenery to form desirable city landscape that describes placeness concretely. In case of Korea, vast harbors and industry complex are generally located in the waterfront of port cities because the waterfront is noticed as one factor which supplies a fresh ground. So, waterfront forms a wide and long blackout curtain that causes the destruction of nature, shapes a factory structure curtain, and completely covers hinterland landscape. It makes hard for citizens to access to the seaside, depriving capacity of waterfront for water-familiar, and destructing beautiful seashore environment. This research project is to provide those architects or planners with the design guidelines of improving port and hinterland landscape. To do so, this study is carried out the physiological evaluation making eye fixation behaviors. The main results were summarized as follows: 1) Satisfaction with the ports' scenery turned out being given high estimation according as the objections being seen is more natural, a scale of ports' landscape elements is larger, and shape of landscape elements are simpler. 2) Distribution of a visual point is concentrated on facilities that have unique shapes and is under the influence of seashore line, letters carved in buildings and conspicuous colors. In addition, it tends to gather a visual point that facility's movement would be expected. 3) Eye fixation behaviors stops at unique shapes, structural lines of the physical elements and color factors of the objects. And it was judged that human recognized the object through this. 4) Scenery that include large natural element has a flexible movement of view and make general populace see the object for more long time. Therefore, it is necessary to examine the vista point on view-distance with research on improving port's naturality.

한우 도체등급에 따라서 발현되는 경제형질 유전자의 유의성 검사

강현아,김상환,진보람,김대승,윤종택 한국동물생명공학회(구 한국동물번식학회) 2011 발생공학 국제심포지엄 및 학술대회 Vol.2011 No.1

쇠고기의 육질 등급을 좌우하는 요인으론 육색, 지방색, 조직감 및 상강도(마블링, 근내 지방도) 등이 있는데 그중 근육 내 지방 축적도는 고기의 연도 및 다즙성에 영향을 미치 며 등급을 결정하는 중요한 요인 중 하나이다. 최근 들어 근육 내 지방 합성에 관련된 유전자 연구 및 SNP 탐색을 통한 DNA maker 개발 등 분자생물학적으로 많은 연구보고 가 있으나 등급 간 유의성에 대한 정확한 증명에 관한 연구는 미비하다. 따라서 본 연구는 한우등급 판정소에서 공인하여 선정된 1++의 등급을 판정받은 한우 6두와 2등급으로 판정받은 한우 6두에서 근내지방도와 관련된 유전자로 알려져 있는 GPAT, ACSS2, MGAT, EXT1, FABP4, BCO2, LPL, DGAT, PLIN2, ADSF, TG와 연도와 관련된 유전자인 CAST와 CAPN3의 mRNA 발현양상을 분석하고 유의적 차이점을 확인 하여 경제 형질 선발 마커의 사용 가능성을 선발하기 위하여 등심조직 내 mRNA를 추출 하였고, 각 유전자를 탐침으로 이용하여 sqRT-PCR 및 양적 분석을 위한 nano-spector mate fluorescence system으로 발현양상을 측정하였다. 이후 유의적 평가를 검증 위하여 SAS program (V9.1)의 t-test 검정을 실시하였다. 각 경제 형질 유용 유전자의 발현양상을 분석한 결과 ADSF와 LPL의 경우 평균 근내 지방도가 8±1인 1++등급보다 2±1인 2등급에서 높은 발현양상을 보이고 있었으며, 그 값 이 유의적 차이를 보이지 않았다. 이 외 근내지방도 및 연도와 관련된 유전자 그룹의 경 우 1++등급이 2등급보다 발현양상이 높게 측정되었다. 근내지방도 및 연도에 관련된 유 전자로 선발한 GPAT, ACSS2, DGAT, PLIN2의 경우 유의성 분석결과 유의적 차이점이 없는 것으로 나타났으며, MGAT, EXT1, CAPN3, FABP4, BCO2, CAST, TG의 경우 유의 성(p<0.05)이 있었다. 따라서 본 연구 결과에 의하여 근내지방도 선발 표지 마커로 사용이 가능한 MGAT, EXT1, TG, FABP4, BCO2와 연도 측정에 관련한 CAST, CAPN3의 총 7개의 유전자를 선발 할 수 있었다.

컨테이너항을 중심으로 한 港灣景觀의 心理的 評價에 관한 硏究

정성구,김은일,신남수,윤진보 대한건축학회 2005 대한건축학회논문집 Vol.21 No.9

The purpose of this study is to explain the psychological analysis about the container port landscape, to make the attractive ocean space. An Ocean space is the very important factor not only to represent urbanity and civic culture, and representative urbanscape but also to estimate environmental quality. However, nowadays, coastal landscape is being changed and destructed by the large scale development in container port area. And there is rare research on port and hinterland landscape and no guideline for port landscape planning and management.This research project is to provide those planners with the landscape management measures for the port and hinterland. To do so, fist of all, the evaluation of examinees' psychological structures to explicate spatial consciousness of the port landscape is attempted by semantic differential method using 18 photo images of Oakland, Hongkong, Osaka, Kobe, Inchon, and Busan port. And then, Factor Analysis, Frequencies, and ANOVA were applied to examine the port landscape examples.Finally, psychological evaluation construction about the port landscape is made on the bases of psychological factors of human. As a result, psychological evaluation factors defining the characteristics of port landscape can be explained by 5 factors; amenity, artificiality, vigorousness, identity and open view.As a result of ANOVA, many difference is represented in psychological characteristics which a amenity, vigorousness, and open view. We expect this research to be used as a valuable data in evaluation and planning of portscape.