한방병원 외래진료부 진료실의 단위공간에 관한 건축계획적 연구

유창승,이특구 한국의료복지시설학회 1998 의료·복지 건축 Vol.4 No.6

After 1980's, more and more people expect to the mysterious treatment of herb medicine about chronic and degenerated disease, according to growth of income level and old population. So the herb medicine hospital is begin to increase rapidly. But, they apply western hospital plan to the herb medicine hospital project which cause a lot of problem. To solve the problem, we suggest the new design standards for the purpose of this thesis which considered treatment method and system of herb medicine hospital.

Multicenter Study on the Molecular Epidemiology of Intestinal Protozoan Parasites in Korea

유창승,오예진,고영진,배미현,변정현,원은정 대한임상미생물학회 2023 Annals of clinical microbiology Vol.26 No.3

Background: Intestinal protozoa are potential diarrhea-causing pathogens and monitored worldwide. The Korea Centers for Disease Control and Prevention has also been monitoring intestinal protozoa causing diarrhea for many years. Recently, the overall protozoa detection rate has decreased to less than 1%, but whether protozoa infection causing diarrhea has declined or is being underestimated has not been studied. This study aimed to investigate the molecular epidemiology of intestinal protozoan pathogens in stool samples collected from multiple Korean centers. Methods: Stool samples were collected from five university hospitals and a commercial laboratory. Direct smear and trichrome staining were performed on all samples. The presence of Cryptosporidium parvum, Giardia lamblia, Entamoeba histolytica, Cyclospora cayetanensis, Dientamoeba fragilis, and Blastocystis hominis were detected using Allplex™ Gastrointestinal Parasite Assays (Seegene Inc., Korea). Microsporidia species and Kudoa septempunctata were detected using PowerChek™ Microsporidia Multiplex and Kudoa Real-time PCR kits (Kogene Biotech, Korea), respectively. Results: The collected samples included 279 diarrheal and 51 non-diarrheal samples. Among the 279 diarrheal samples, nine samples [B. hominis (n=7), C. parvum (n=1), and Microporidia species (n=1)] were positive, but there were no positive samples for K. septempunctata. We could not detect any protozoa by direct smear and trichrome staining. Among the 51 nondiarrheal samples, 10 (19.6%) samples were positive for B. hominis, but no other protozoa were observed. Conclusion: This multicenter study showed that the detection rate of intestinal protozoa is currently low in diarrheal samples from Korea. However, B. hominis was frequently detected in non-diarrheal samples, indicating their low pathogenicity.

결핵성 수축방광으로 오인한 방광이행상피세포암

유창승,설승훈,장윤형,박연원,이진형,민승기 대한비뇨의학회 2006 Investigative and Clinical Urology Vol.47 No.1

Bio-Rad D-100의 헤모글로빈 A1c 분석능 평가

유창승,최은혜,배인철,이상국,김정호 대한진단검사의학회 2017 Laboratory Medicine Online Vol.7 No.2

Background: Hemoglobin A1c (HbA1c) is considered a marker useful for the follow-up and diagnosis of diabetes and implies the importance of reliable assay methods that are traceable to a reference method. We evaluated analytical performance of a new high-performance liquid chromatography system for the HbA1c assay: D-100 from Bio-Rad Laboratories (USA). Methods: We evaluated precision, linearity, and carry-over of D-100, according to the Clinical and Laboratory Standards Institute’s guidelines. Comparative analysis of D-100 with Integra 800 (Roche Diagnostics, Germany) and Capillarys 3 (Sebia, France) was conducted. Additionally, we evaluated the throughput of the three instruments. Results: Precision of low- and high-concentration controls in D-100 showed a CV of less than 1%. The linearity was excellent (R2=0.999) in the range of 3.51-18.7%, and carry-over was not observed. HbA1c results of D-100 (n=144) showed good correlation with those of Integra 800 (r=0.993) and Capillarys 3 (r=0.996). The % bias between D-100 and Integra 800 or Capillarys 3 was within the allowable range at all 3 medical decision levels (5.7%, 6.5%, and 10.0%). Elapsed time in the analysis of the first sample by D-100 was shorter than that of Integra 800 (2.4 vs. 11.1 minutes), but subsequent samples took more time (0.8 vs. 0.3 minutes per sample). Conclusions: D-100 showed reliable analytical performance with good precision and linearity, minimal carry-over, and acceptable comparative characteristics relative to other instruments. D-100 is expected to be useful for clinical measurements of HbA1c for diabetes diagnosis and theranostics. 배경: 헤모글로빈 A1c (HbA1c)는 당뇨의 진단과 치료, 추적 관찰에 유용한 지표로서 표준화된 검사법에 의한 정확한 검사 결과를 제공하는 것이 중요하다. 본 연구에서는 고성능 액체크로마토그래피법(HPLC)을 이용하여 HbA1c를 측정하는 장비인 D-100 (Bio-Rad Laboratories, USA)의 분석 성능을 평가하고자 하였다. 방법: Clinical and Laboratory Standards Institute (CLSI)의 지침에따라 D-100의 정밀도와 직선성, 검체 간 교차오염률을 평가하였다. 그리고 D-100과 기존 운용장비인 Integra 800 (Roche Diagnostics, Germany), Capillarys 3 (Sebia, France)와의 상관성 및 일치도를 조사하였다. 마지막으로 세 검사법의 검사 소요 시간을 비교 분석하였다. 결과: D-100을 사용한 HbA1c 측정의 검사차례 내 정밀도와 총 정밀도는 저농도와 고농도 모두에서 1% 미만의 변이계수를 보였다. 직선성은 HbA1c 3.51-18.7%에서 우수하였고 검체 간 교차오염률은 관찰되지 않았다. D-100의 Integra 800과 Capillarys 3에 대한 상관성 평가(n=144)에서 상관계수는 각각 0.993, 0.996으로 높은 상관성을 보였으며 세 가지의 임상적 의사결정 농도(5.7%, 6.5%, 10.0%) 모두에서 바이어스(%bias)는 허용범위 이내에 존재하였다. 검사 소요시간은 세 장비의 특성에 따라 차이가 있었다. D-100은 Integra 800에 비해 최초 검체를 더 빨리 처리하였으나(2.4분 vs. 11.1분) 연속 검체 처리시간은 더 길었다(0.8분 vs. 0.3분). 결론: D-100은 우수한 정밀도와 직선성 및 기존의 장비와의 비교에서 높은 상관성과 빠른 검체 처리속도를 보여주었다. 임상적으로 당뇨병의 진단과 치료, 당뇨 합병증의 예방을 위한 추적 관찰에 유용하게 사용할 수 있을 것으로 예상된다.

한국의 정상 성인에서 연령에 따른 PSA의 변화 양상

유창승,이진형,김청수 대한비뇨의학회 2007 Investigative and Clinical Urology Vol.48 No.8

새로운 말디토프 질량 분석 장비인 ASTA MicroIDSys의 임상 균주를 이용한 균종 동정 성능 평가

유창승,이은정,김도균,정석훈 대한임상미생물학회 2020 Annals of clinical microbiology Vol.23 No.3

Background: We evaluated the performance of ASTA MicroIDSys (ASTA, Korea) and Bruker Biotyper (Bruker Daltonics, Germany) systems in the identification of bacterial isolates from clinical microbiology laboratory specimens during the study period. In addition, species for which the identification accuracy using MALDI-TOF MS systems was previously reported to be poor were also identified by comparing the MS results with those obtained using molecular identification. Methods: A total of 889 non-duplicated clinical isolates were included in this study. The results of ASTA MicroIDSys were compared with those of Bruker Biotyper; 16S rRNA sequencing was performed for the species for which results obtained using the two systems did not match. The sequences of rpoB , hisA, and/or recA for the clinical isolates of Acinetobacter species, Klebsiella species, and Burkholderia cepacia complex were analyzed and used as reference identifications. Results: The concordance rates for bacterial identification using ASTA MicroIDSys and Bruker Biotyper were 100% at the genus level and 98.3% at the species level for isolates belonging to the order Enterobacterales. Similarly, the concordance rates at the genus and species levels were 98.8% and 91.0% for glucose non-fermenting bacilli, 100% and 100% for gram-positive cocci, and 98.9% and 98.9% for other isolates, respectively. ASTA MicroIDSys was expected to correctly identify 97.9% of the 108,251 isolates identified in our clinical microbiology laboratory over the past 5 years. Conclusion: ASTA MicroIDSys showed excellent performance in bacterial identification for most of the clinically relevant species. Further extension of the database could improve the identification accuracy of ASTA MicroIDSys.

TCU테스트실행기개발및유효성검증연구

유창승(Chang-Seung Yu),정기현(Ki-Hyun Jung),최경희(Kyung-Hee Choi) 한국통신학회 2012 한국통신학회 학술대회논문집 Vol.2012 No.2

키와 체중, 신체질량지수가 젊은 남성의 정계정맥류에 미치는 영향

설승훈,이진형,유창승,임종완,민승기,장윤형,박연원 대한남성과학회 2006 The World Journal of Men's Health Vol.24 No.3

Clinical and Microbiological Risk Factors for Community-Associated Clostridioides difficile Infections

김영아,김희정,김도균,유창승,정석훈 대한임상미생물학회 2022 Annals of clinical microbiology Vol.25 No.2

Background: The incidence of community-associated (CA) Clostridioides difficile infection(CDI) has increased in Korea. In this study, we evaluated CA-CDI risk factors in terms of clinicalfeatures and ribotype considering its region-specific molecular epidemiology. Methods: A retrospective case-control study was performed on two groups of CDI patients:127 subjects with CA-CDI and 265 subjects with healthcare-associated (HA)-CDI. Risk factorsfor CA-CDI were evaluated in terms of clinical and microbiological features such as toxin typeand ribotype. Results: A comparison of the two groups of CDI patients revealed that inflammatory boweldisease, diarrhea, abdominal pain, and fever were more closely associated with CA-CDI. Thetoxin types and ribotypes of C. difficile were similar between the two groups. After adjustingfor variables, no risk factors were identified for CA-CDI compared with HA-CDI. Conclusion: Specific risk factors for CA-CDI were not identified in this study.

Epidemiology and Subtype Distribution of Blastocystis Among Asymptomatic Volunteers in South Korea: A Multicenter Study

김성훈,변정현,오예진,유창승,배미현,원은정 대한임상미생물학회 2023 Annals of clinical microbiology Vol.26 No.1

Background: Blastocystis is a genus of intestinal, anaerobic protozoan parasites that can be isolated from humans, animals, and the environment. We aimed to determine the distribution of Blastocystis and subtypes (STs) using stool samples obtained from healthy volunteers at collection centers in South Korea. Methods: A total of 478 stool samples from volunteers were collected at five collection centers throughout South Korea. The presence of Blastocystis was determined using PCR based on the small subunit (SSU) rRNA gene, and Blastocystis STs were confirmed through sequencing of the SSU rRNA gene. Results: Molecular analysis revealed the presence of Blastocystis in 27 (5.6%) of the enrolled participants. Two STs were identified: ST3 (66.7%) and ST1 (33.3%). The positive rates of Blastocystis varied by geographical region, ranging from 1.2%–12.0%. ST3 was the predominant subtype in all centers except one, where only ST1 was isolated. Phylogenic analysis showed clustering based on ST, but no significant differences were found among the regions. There was no association between Blastocystis colonization and either age or sex of the participants. Conclusions: The results of this multicenter study demonstrated colonization by Blastocystis, mainly ST3, in the gastrointestinal tracts of asymptomatic individuals in South Korea.