Procarbamate계 살충제 benfuracarb의 산화적 활성화 과정을 통한 독성발현

유용만(Yong-Man Yu),김은향(Eun H. Kim),김성문(Songmun Kim),허장현(Jang-Hyun Hur) 한국농약과학회 2003 농약과학회지 Vol.7 No.1

엽채류 및 과채류의 재배유형 및 유통경로별 생물학적 위해요소 조사

유용만 ( Yong Man Yu ),윤영남 ( Young Nam Youn ),최인욱 ( In Uk Choi ),이영하 ( Young Ha Lee ),( Xiang Long Yuan ) 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2007 한국식품저장유통학회지 Vol.14 No.1

Glutathione-S-transferase와 esterase 효소 저해특성을 이용한 농약의 혼합 상승효과

유용만(Yong-Man Yu),홍순성(S. S. Hong),김성문(S. M. Kim),허장현(J. H. Hur) 한국농약과학회 2003 농약과학회지 Vol.7 No.1

실내조건에서 친환경농자재가 기생성 천적곤충에 미치는 영향

유용만,강은진,서미자,강명기,이희진,김다아,길미라,윤영남,Yu, Yong-Man,Kang, Eun-Jin,Seo, Mi-Ja,Kang, Myeng-Gi,Lee, Hee-Jin,Kim, Da-A,Gil, Mi-La,Youn, Young-Nam 한국응용곤충학회 2006 한국응용곤충학회지 Vol.45 No.2

시설하우스의 주요 난방제 해충인 온실가루이, 진딧물 및 아메리카잎굴파리의 생물적 방제를 위해 사용되는 온실가루이좀벌, 콜레마니진디벌 및 굴파리좀벌 굴파리고치벌에 대한 친환경농자재 사용에 따른 영향을 조사하였다. 먼저 친환경농자재가 천적과 직접 접촉하였을 경우의 영향을 알아보기 위하여, 온실가루이좀벌의 머미에 분무하였을 경우, 61개 품목 중에서 살균성친환경농자재인 FEFAME (0.4%), 몰리브덴함유친환경농자재 G (2.7%)를 뿌린 경우에는 거의 우화하지 않았다. 또한, 성충에 미치는 잔효독성을 조사한 결과, 식물성추출물친환경농자재 J 와 몰리브덴함유친환경농자재인 EFAMMo C는 처리 후 48시간 후의 생존율이 0%였고, 미량요소함유친환경농자재인 EFAMME B (6.7%)., 식물성추출물친환경농자재인 EFAMPE H, 토양미생물친환경농자재인 EFAMSM H (13.3%), 살균성친찬경농자재인 FEFAM A와 D (20.0%) 등이 처리 48시간 후 생존율이 낮게 나타났다. 콜레마니진디벌의 경우 직접접촉에 대한 평가에서 머미에 분무하는 경우에는 대조구(물)조차도 22%의 낮은 우화율을 보이고 있으며, 성충에 대한 잔효효과의 경우에, 몰리브덴함유친환경농자재인 EFAMMo C는 처리 48시간 후 0%의 생존율을 나타내었고, 식물성추출물친환경농자재인 EFAMPE J (6.7%), EFAMPE F 와 EFAMPE H, 토양미생물친환경농자재인 EFAMSM H (13.3%)등이 20% 이하의 낮은 처리 후 48시간 후의 생존율을 나타내었다. 굴파리좀벌 굴파리고치벌의 경우에는 식물성추출물친환경농자재인 EFAMPE J가 처리 후 48시간 후 0%의 생존율를 나타내었고, 몰리브덴함유친환경농자재인 EFAMMo C가 53.3%로 낮은 처리 후 48시간 후의 생존율을 보였으며, 이외 친환경농자재에 대하여는 영향이 거의 없거나 낮은 것으로 나타나고 있다. For the biological control of the greenhouse whitefly, aphids, American leaf-miners, Encarsia formosa, Aphidius coiemani, Diglyphus isaea, and Dacnusa sibirica were used as biological control factors. Otherwise, many kinds of environment friendly agricultural materials were also used in the kindly environment friendly farming. For testing the toxicity of environment friendly agricultural materials against to insect parasitoids as biological control factors, 61 environment friendly agricultural materials were selected by using methods and periods with insect parasitoids in the greenhouses. Environment friendly agricultural materials were sprayed to mummies and adults of E. formosa and A. colemani, and adults of Diglyphus isaea and Dacnusa sibirica in laboratory condition. Emergence rates were checked during 12 days after spray to mummies of E. formosa and A. colemani, and survival rates were at 24 and 48 hours after viral test for adult parasitoids, with 10% sugar solution. Emergence rates of E. formosa mummies were inhibited by sprayed the fungicidal environment friendly agricultural material (FEFAM) E and the environment friendly agricultural materials contained molybdenum (EFAMMo) G as 0.4 and 2.7%, respectively. E. formosa adults were not survived in vial for 48hours after sprayed and dried with the environment friendly agricultural materials contained plant extract (EFAMPE) J and the EFAMMo C. The environment friendly agricultural materials contained microelements (EFAMMEs) B and EFAMPE H, environment friendly agricultural materials contained useful soil microorganisms (EFAMSMs) H, FEFAM A and FEFAM D were recorded low survival rates of E. formosa adults with 6.7, 13.3, 13.3, 20.0 and 20.0, respectively. In case of the spraying environment friendly agricultural materials to mummies of A. colemani there were low emergence rate from mummies to adults as around 20s % with water spraying as control. A. colemani adults were not survived in vial with EFAMMo C. EFAMPE J, EFAMPE F, EFAMPE H, EFAMSM H were recorded low survival rates under 20% as 6.7, 13.3, 13.3 and 13.3, respectively, D. isaea and D. sibirica adults were not survived in vial with EFAMPE J. EFAMMo C was 53.3% of survival rates in 48 hours vial tests. D. isaea and D. sibirica were not affected by environment friendly agricultural materials compared with E. formosa and A. colemani.

감자 흑각병원균 Erwinia carotovora subsp. atroseptica의 화학적 방제

유용만(Yong-Man Yu),Zhu Yong-zhe,배후남(Hu-Nam Bae),김성문(Songmum Kim),임춘근(Chun-Keum Lim),허장현(Jang-hyun Hur) 한국농약과학회 2003 농약과학회지 Vol.7 No.1

재배방식에 따른 딸기의 부위 별 세균 오염도 분석

유용만 ( Yong Man Yu ),김진원 ( Jin Won Kim ),최인욱 ( In Wook Choi ),윤영남 ( Young Nam Youn ),이영하 ( Young Ha Lee ) 한국식품저장유통학회(구 한국농산물저장유통학회) 2013 한국식품저장유통학회지 Vol.20 No.3

딸기는 다양한 효능을 보일 뿐만 아니라 맛과 영양까지 뛰어나 많은 사람들이 선호하는 대표적인 즉석섭취 농산물중의 하나이다. 따라서 딸기의 안정성에 대한 관심이 매우 높다. 본 연구는 딸기의 생물학적 안전성을 평가하기 위하여 논산시내 12개 딸기 농장에서 채취한 1020개의 표본(열매, 줄기, 잎 각각 340개씩)을 대상으로, 재배방식(양액재배, 무농약재배, 유기농재배) 및 딸기의 부위에 따른 총호기성균, 대장균군/대장균을 정량적 분석하였다. 또한 식중독유발 세균인 황색포도상구균, Salmonella spp, L monocytogenes, E. coli O157: H7 오염 여부를 정량 혹은 정정 분석하였다. 딸기 표본의 총호기성균 수는 2.3~6.8log10 CFU/g범위이었으며, 대장균군은 전체 표본의 14.2%에서 검출되었고 양성 표본의 대장균군 수는 2.1-4.5log10 CFU/g범위이었다. 대장균은 전체 표본중 1.0%에서 검출되었고, 양성 표본의 대장균 수는 2.1-2. 8log10 CFU/g범위이었다. 딸기의 재배방식별로 분석시, 총호기성균 및 대장균군의 수는 양액재배 혹은 무농약재배에 비하여 유기농재배에서 더 많이 검출되었으나(p<0.05), 대장균은 무농약재배와 유기농재배에서 유사하게 검출되었고 영양재배에서는 검출되지 않았다. 딸기를 3개 부위(열매, 줄기 및 잎)로 나누어 호기성균 및 대장균군/대장균 수를 분석시, 이들간 통계적 유의성은 없었다. 또한 딸기에 부착도어 있는 식중독 유발 세균을 조사한 결과, 1020개의 표본중에서 유기농재배 농가 표본 2개 에서 황색포도상구균이 검출되었으며 그 외의 Salmonella spp., Listeria spp., E coli 0157: H7은 표본에서 검출되지 않았다. 이상의 결과로 보아, 딸기의 재배 방식에 따라 위생지표 미생물의 오염에 차이가 있을 수 있으므로, 재배방식에 적합한 세균 오염 저감화 대책이 요구됨을 시사하겠다. Strawberries are among the leading ready-to-eat agricultural products that have superior taste and nutrition. Thus, consumer concerns about the safety of eating strawberries are growing. To evaluate the contamination levels of strawberries according to their cultivation methosds (nutriculture, pesticide-free culture and organic farming) and parts [fruit (flesh), stalk (pedunle) and leaf (calyx)], 1,020 parts of strawberry samples were collected at 12 farms in Nonsan-si and quantitatively or qualitatively examined for the indicators of food safety and food poisoning bacteria. The total aerobic bacteria count in the whole samples was 2.3~6.8 log10 CFU/g, and coliform bacteria were detected in 14.2% of the whole samples with a contamination level range of 2.1~4.5 log CFU/g. E. coli were detected in 0.9% of the whole samples with a contamination level range of 2.1~2.8 log CFU/g. The analysis of the bacterial levels according to the cultivation methods showed that the total aerobic bacteria and coliform counts were higher in the strawberries that were grown via organic farming than in those that were grown via nutriculture and pesticide-free culture. However, the E. coli counts of the strawberries that were grown via organic farming and via pesticide-free culture were similar and differed from that of the strawberries that were grown via nutriculture. The analysis of the contamination levels according to the parts of the strawberries showed that the total aerobic bacteria, coliform and E. coli counts of the fruits, stalks and leaves of the strawberries did not significantly differ. Staphylococcus aureus was detected in two organically grown strawberries, but Salmonella spp., Listeria monocytogenes and E. coli O157:H7 were not detected in the whole samples. These results show that the bacterial contamination levels of the strawberries differed based on their cultivation methods. Thus, a suitable method of reducing the bacterial contamination levels of strawberries according to their farming methods is needed.

파프리카의 미생물 모니터링 및 보관온도에 따른 세균오염도 분석

유용만 ( Yong Man Yu ),윤영남 ( Young Nam Youn ),최인욱 ( In Uk Choi ),이영하 ( Young Ha Lee ) 한국식품저장유통학회(구 한국농산물저장유통학회) 2011 한국식품저장유통학회지 Vol.18 No.1

Paprika is a major export of Korea, but biosafety is important if exports are to grow. To date, few paprika biosafety data are available. We evaluated microbiological contamination of paprika, and determined bacterial levels with respect to storage temperature. Mean counts of total aerobic bacteria were 2.3±0.3 log10 CFU/g, but coliforms were not isolated. Staphylococcus aureus, Salmonella spp., Listeria spp., and Escherichia coli O157 were also not detected. When bacterial contamination of paprika stored at room temperature or 4℃ for 20 days was evaluated, the numbers of total aerobic bacteria peaked at 14-16 days at room temperature (4 times more than those of 4℃). However, aerobic bacteria grew slowly at 4℃. Coliforms were also detected twice at room temperature, but not upon storage at 4℃. The results indicate that the paprika exported from Korea is relatively biosafe. However, food hygiene issues must be addressed to reduce contamination during storage and distribution.

담배가루이(Bemisia tabaci) 병원성곰팡이 Lecanicijjium lecanii Btab0l 균주의 특성

윤여준,유용만,이민호,한은정,홍성준,안난희,김용기,지형진,박종호,Yoon, Yeo-Jun,Yu, Yong-Man,Lee, Min-Ho,Han, Eun-Jung,Hong, Sung-Jun,Ahn, Nan-Hee,Kim, Yong-Ki,Jee, Hyung-Jin,Park, Jong-Ho 한국응용곤충학회 2010 한국응용곤충학회지 Vol.49 No.4

국내에 문제가 되는 담배가루이(Bemisia tabaci)의 살충성 Lecanicillium lecanii Btab01 균주의 생육 특성과 살충력을 검정하였다. PDA, TSA, SDA+Y배지에서 L. lecanii Btab01은 $20{\sim}25^{\circ}C$에서 균사성장이 가장 빨랐으며 $28^{\circ}C$이상에서 성장이 억제되었다. L. lecanii Btab01는 고체배양과 액체배양 시 담배가루이 약충에 대해 살충률에 큰 차이를 보이지는 않았다(93.9%, 98.3%). 농도별로는 $10^5$, $10^6$, $10^7$, $10^8$ cfu/ml에서 각각 5.8%, 33.8%, 77.3%, 98.5%의 살충률을 보였고 각각 16.1일, 7.3일, 5.1일, 3.5일의 $LT_{50}$값을 나타내었다. L. lecanii Btab01을 약충에 처리 후 포화습도 조건을 0 시간 24 시간, 168 시간 유지하였을 때, 0%, 20.3%, 100%의 살충률을 보였다. 균주에 0.5% 식용유를 첨가하였을 때 포자의 발아가 2 배 가량 증가하였으며 균주에 0.1% 식용유를 첨가하여 약충에 처리했을 때(98.6%)가 첨가하지 않았을 때(79.9%)보다 살충력이 향상됨을 알 수 있었다. Cultural characteristics Lecanicillium lecani Btab01 and its insecticidal activity against tobacco whitefly (Bemisia tabaci) were investigated. On potato dextrose agar, tryptic soy agar and SDA+Y media, mycelial growth of L. lecani Btab01 was best at $20{\sim}25^{\circ}C$ and suppressed above $28^{\circ}C$. Both solid culture and liquid culture of L. lecani Btab01 showed high insecticidal activity, 93.9 and 98.3% respectively, against nymph of tobacco whitefly, but there is no significant difference. When culture of L. lecani Btab01 was treated at the concentration of $10^5$, $10^6$, $10^7$ and $10^8$ cfu/ml, their insecticidal activity were 5.8%, 33.8%, 77.3% and 98.5% respectively, and $LT_{50}$ values were 16.1 days, 7.3 days, 5.1 days and 3.5 days respectively. When nymphs were treated by the cultures of L. lecani Btab01 and maintained under saturated condition for zero hour, 24 hours and 168 hours, their control activities were 0%, 20.3% and 100% respectively. Spore germination of L. lecani Btab01 was increased about two times by adding edible oil. When L. lecani Btab01 was treated to control nymph with 0.1% edible oil, it showed high control activity(98.6%) compared to single treatment of L. lecani Btab01 (79.9%).

감자흑각병(Potato Blackleg Disease) 방제를 위한 살균제 선발

Zhu Yong-Zhe,박덕환(Duck-Hwan Park),박동식(Dong-Sik Park),유용만(Yong-Man Yu),김성문(Songmun Kim),임춘근(Chun-Keum Lim),허장현(Jang-Hyun Hur) 한국농약과학회 2003 농약과학회지 Vol.7 No.2

딸기 시설재배지에서 점박이응애의 발생과 분포

최용석(Yong-Seok Choi),황인수(In-Su Hwang),김규상(Kyu-Sang Kim),조효려(Hyo-Ryu Jo),서정학(Jeong-Hak Seo),박덕기(Deok-Gee Park),이영수(Young-Su Lee),유용만(Yong-Man Yu) 충남대학교 농업과학연구소 2014 농업과학연구 Vol.41 No.4

Mean densities of two-spotted spider mite (TSM), Tetranichus urticae Koch adults from January to April in 2014 to investigate the occurrence and dispersion pattern of T. urticae on purpose of developing a monitoring method in the strawberry PVC house. Difference of density of T. urticae adult on middle and both side of leaf wasn’t significantly. Density of T. urticae in investigation at different furrows and investigating points was the highest at the right and left sides of a furrow in strawberry PVC house. In results of comparing the dispersion incides of Taylor’s power law (TPL) with Iwao’s patchiness regression (IPR), TPL described better mean-relationship for the dispersion indieces compared to IPR. Slopes and intercepts of TPL from leaf samples did not differ among surveyed regions. Also, Distribution of T. urticae in a strawberry PVC house was the gravitation of the distribution because b and β values of TPL and IPR was bigger than 1.