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한국 성인여성의 심미적 상악절치 위치에 관한 두부방사선 계측학적 연구
유보영,김종철 전남대학교 치과대학 1991 전남치대논문집 Vol.3 No.1
Esthetic is one of the prime goal of the orthodontic treatment. The upper incisor position is important because it is decisive in balanced smile. This study was attemped to obtain the standards and equations on the esthetic upper incisor position. Lateral cephalometric rediographs of 66 Korean young adult females who were candidates of Miss Korea Beauty Contest were traced, measured and statistically analyed. The results were as follows ; 1. The mean and standard deviation of the measurement for upper incisor position were obtained in the Korean Beauty. 2. Among the measurements for the axis of upper incisor,1_ to Sn and 1_ to FH were highly correlated with FH-MP, ANB, FH-NPog, NAPog, Y-axis, but 1_ to SN(Is-Pr) to present axial inclination of labial surface of upper incisor didn't have any correlation with the measurements for dentofacial pattern. 3. Among the measurements for the vertical position of upper incisor, 1_ to stom had low correlation with the measurements for dentofacial pattern and 1_ to PP(mm) was highly correlated with the measurements for facial growth direction. 4. Sixteen multiple regression equations were established to predict esthetic upper incisor position balancing with dentofacial pattern. 5. It doesn't have much power of persuation to predict esthetic upper incisor position from the measurements for dentofacial pattern.
사람태반의 FAD 의존성 α-Glycerophosphate 탈수소효소의 정제 및 성상
유보영,이희성 중앙대학교 의과대학 의과학연구소 1981 中央醫大誌 Vol.6 No.4
The distribution and some properties of FAD-linked α-glycerophosphate dehydrogenase of human term placenta have been studied. Human placenta was fractionated by differential centrifugation into nuclear, mitochondrial and cytosolic fractions. The activity of mitochondrial FAD-linked α-glycerophosphate dehydrogenase(cytochrome oxidoreductase, EC 1.1.99.5) was measured by the procedure of Gonzalez-Cerozo and Dalziel. The activity in homogenate was found to be 33 units per g wet tissue and was recovered about 87% in mitochondrial fraction (specific activity: 2.02 units/mg protein). Cytosolic NAD-linked α-glycerophosphate dehydrogenase (NAD^+ 2-oxidoreductase, EC 1.1.1.8) activity was assayed by the method of Dawson and Thorne. The activity in homogenate was 25 units per g and was recovered about 96% in cytosolic fraction(specific activity: 0.57 units/mg protein). Thus, the specific activity of FAD-linked dehydrogenase in mitochondria was to be about 3.5-fold greater than that of NAD-linked dehydrogenase in cytosol. Mitochondrial FAD-linked α-glycerophosphate dehydrogenase from human placenta has been purified about 139-fold by osmotic shock, solubilization with Triton X-100, and DEAE-cellulose column chromatography. The optimal temperature for enzyme activity was 38℃ and the optimal pH was 7.6. The Km value for α-glycerophosphate was 9.34mM. The molecular weight was estimated to be 320,000±10,000 by polyacrylamide disc gel electrophoresis. The half life of the enzyme activity was 5 min at 50℃. At the concentration of 1.0×10^-4M of magnesium ion and clacium ion, the enzyme activities of α-glycerophosphate dehydrogenase were increased to 35% and 8%, respectively. However, at the greater concentration over 1.0×10^-4M of magnesium ion and calcium ion, the enzyme activities were markedly reduced.
Effect of mesenchymal cells on human hair growth and death
유보영,박정극,서영권,윤희훈,Youn-Ho Shin,송계용 한국화학공학회 2008 Korean Journal of Chemical Engineering Vol.25 No.2
Animals have typically been used in efficacy tests, but there are a number of dissimilarities between humans and animals. To overcome the problems associated with animal testing, a model which is reproduced in vitro with longterm culture with cell growth with in vivo activity must be developed. We made a gel-type dermal equivalent (DE) that contained dermal papilla cells (DPCs) or dermal sheath cells (DSCs) isolated from human hair bulbs in order to mimic human scalp tissue. Hair follicles were organ-cultured on DE containing DPCs or DSCs. The DE used for organ culture was a reconstructed 3-dimensional contraction of collagen gel, and the cell density of the DE did not affect the increase in hair length. We tested the effects of cell types in DE on increases in hair length, and the results showed a large increase in hair length and long-term viability in the air-liquid interface culture on DE containing DSCs. We compared the submerged culture with the hair air-liquid interface culture on DE using immunohistochemical staining, and found that the hair follicles that were air-liquid interface cultured on DE maintained the growth phase (anagen) for a longer period of time than the hair follicles that were submerged. Since the hair follicles were cultured under an air-liquid interface condition, the increase in hair length was a reflection of the epithelial cell growth that resulted from the improved oxygen supply and paracrine factors secreted from hair origin cells.