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Identification of Putative MAPK Kinases in Oryza minuta and O. sativa Responsive to Biotic Stresses
유민경,Seung-Ick Oh,옥승한,Sung Ki Cho,신현영,Ji Ung Jeung,신정섭 한국분자세포생물학회 2007 Molecules and cells Vol.23 No.1
The mitogen-activated protein kinase (MAPK) signaling cascade is critical for regulating plant defense systems against various kinds of pathogen and environmental stresses. One component of this cascade, the MAP kinase kinases (MAPKK), has not yet been shown to be induced in plants following biotic attacks, such as those by insects and fungi. We describe here a gene coding for a blast (Magnaporthe grisea)- and insect (Nilaparvata lugens)-responsive putative MAPK kinase, OmMKK1 (Oryza minuta MAPKK 1), which was identified in a library of O. minuta expressed sequence tags (ESTs). Two copies of OmMKK1 are present in the O. minuta genome. They encode a predicted protein with molecular mass 39 kDa and pI of 6.2. Transcript patterns following imbibition of plant hormones such as methyl jasmonic acid (MeJA), ethephone, salicylic acid (SA) and abscisic acid (ABA), as well as exposure to methyl viologen (MV), revealed that the expression of OmMKK1 is related to defense response signaling pathways. A comparative analysis of OmMKK1 and its O. sativa ortholog OsMKK1 showed that both were induced by stress-related hormones and biotic stresses, but that the kinetics of their responses differed despite their high amino acid sequence identity (96%).
중년여성의 발달현상에 대한 태도와 갱년기증상 호소, 지각된 건강상태와의 관계 분석연구
유민경,지성애 중앙대학교 의과대학 의과학연구소 1986 中央醫大誌 Vol.11 No.4
This study was done for the purpose of analyzing the relationships between middle-aged women's attitudes toward millde adulthood developmental changes, their self-reported climacteric symptoms, and perceived health status. The hypotheses tested were that middle-aged women's attitudes toward middle adulthood developmental changes are negatively related to the degree of their self-reported climacteric symptoms, attitudes toward middle adulthood developmental changes are positively related to the perceived health status and degree of their self-reported climacteric symptoms are negatively related to the perceived health status. The 197 middle-aged womens who have lived in Seoul were selected conveniently and data were gathered by structured questionaire from August to September, 1986. The instruments used for this study were Middle-Aged Women's Developmental Attitude Scale, the Climacteric Symptoms Scale and Perceived Health Status Scale developed by authors. Analysis of data was done by Pearson's Correlation Coefficient, ANOVA and t-Test. The results of this study were as follows; 1. Attitudes toward their middle adulthood developmental changes was negatively related to the degree of their self-reported climacteric symptoms(r=-.2501, p<.001). 2. Attitudes toward their middle adulthood developmental changes was positively related to the perceived health status(r=.3797, p<.001). 3. The degree of their self-reported climacteric symptoms was negatively related to the perceived health status(r=-.5552, p<.001). 4. Influence of demographic variables on the middle-aged women's attitudes toward middle adulthood developmental changes, their self-reported climacteric symptoms and perceived health status is as follow; Marital satisfaction was positively related to the middle-aged women's attitudes toward their middle adulthood developmental changes(r=.1838, p<.01) and perceived health status(r=.2941, p<.001) and negatively related to the degree of their self-reported climacteric symptoms(r=.2392, p<.001). Those who have experience of treatment and counselling for climacteric symptoms were revealed to have lower degree of their self-reported symptoms(t=34.69, df=196, p<.001) and higher perceived health status(t=55.06, df= 196, p<.001) than those who have not. Those who have higher family income was revealed to have more positive attitudes toward middle adulthood developmental changes than those who have lower (F= 2.354, p<.05). Those who have religion was revealed to have higher perceived health status(F = 2.143, p<0.5) than those who have not.
유민경,정모경,김기은,권아름,채현욱,김덕희,김호성 대한소아내분비학회 2015 Annals of Pediatirc Endocrinology & Metabolism Vol.20 No.3
Purpose: In male patients with congenital adrenal hyperplasia (CAH), the presence of testicular adrenal rest tumors (TARTs) have been reported, however their prevalence and clinical manifestations are not well known. Untreated TARTs may lead to testicular structural damage and infertility. This study was conducted to investigate the prevalence of TARTs in male patients with CAH, and characterize the manifestations to identify contributing factors to TART. Methods: Among 102 CAH patients aged 0–30 years, 24 male patients have been regularly followed up in our outpatient clinic at Severance Children’s Hospital from January 2000 to December 2014. In order to reveiw the characteristics of TART patients, we calculated the mean levels of hormones during the 5 years before the time of investigation. Five patients underwent follow-up scrotal ultrasonography (US) after adjusting the dosage of glucocorticoids. Results: TARTs were detected in 8 of the 13 patients (61.5%). The median age of TARTs diagnosis was 20.2 years with the youngest case being 15.5 years old. The mean serum level of adrenocorticotropic hormone (ACTH) was higher in the TARTs patient group compared to the non-TARTs group (P<0.05). The tumor size decreased in 3 cases, slightly increased in 1 case, and had no change in another case. Conclusion: The serum ACTH level might be associated with the growth promoting factor for TARTs, but the exact mechanism has not been clearly identified. Screening for TARTs using US is important in male patients with CAH for early-detection and prevention of ongoing complications, such as infertility.
Pilot observation of some brain regions with Scanning Acoustic Microscope
유민경,김다솜,유임주 대한체질인류학회 2021 대한체질인류학회 학술대회 연제 초록 Vol.64 No.-
Currently, ultrasound images are being actively used in the field of medical imaging, but limited studies reported microscopic resolution images of biomedical samples. The Scanning acoustic microscope (SAM) can scan in the x-, y- and diagonal directions, which is useful for analyzing details of microstructure in the field of materials science. but there are little studies that analyze biological tissues with SAM. In this study, we studied to try out brain sample of three different regions to confirm the possibility of studying brain tissue with SAM for pilot study.; the pineal gland contained calcium ingredient, the hypothalamic area where white matter and gray matter are clearly separated, and the thalamus where white matter and gray matter are mixed. Experiments were conducted using SAM, We clearly observed calcified structures, known as brain sand in the pineal gland. Also, mammillothalamic tract which goes through hypothalamus was observed clearly. It was possible to obtain images that distinguish between high-lipid white matter and low-lipid gray matter. However, the coronal section of thalamus was a mixture of white and gray matter, and it was not easy to distinguish cytoarchitecture clearly. Through this experiment, we checked the possibility that SAM can be used to analyze the brain cytoarchitecture based on different impedances that make it up. In addition, there is an advantage that less elements are required for sample pre-treatment as compared with conventional microscopic processes without sectioning, which warrants some potential application for the three-dimensional analysis of the brain cytoarchitecture.