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유근제,양지훈,김재훈,황상일,Yoo, Keunje,Yang, Jihoon,Kim, Jae Hoon,Hwang, Sang-Il 한국지하수토양환경학회 2016 지하수토양환경 Vol.21 No.4
Land contamination has emerged as a major environmental and land management issue over the last decade. Although the importance of contaminated land management was continuously increased and many developed countries tried to make advanced contaminated land register system, current Korea soil regulations and policies have not been considered yet. This study analyzed existing or developing contaminated land register system from various countries to suggest implications of environmental decision support. Through this study, the introduction of contaminated land management system as creating a new system in Korea needs to considerable review to the following in order to achieve the objective through a effective adoption and operation (1) we need to establish contaminated land register system by providing a proper legal basis before the imposition of data collection, investigation, and management, (2) sufficient examination is required to identify scope of information disclosure and criteria, contents, and subjects of items from contaminated land register system.
화학사고시 토양오염 사전관리제도 도입을 위한 국내외 제도 분석 및 시사점
유근제,양지훈,황상일,Yoo, Keunje,Yang, Jihoon,Hwang, Sang-Il 한국지하수토양환경학회 2016 지하수토양환경 Vol.21 No.4
Although a number of chemical accidents have been occurred in South Korea, the effective prevention act for soil contamination has not been established so far. To effectively protect soil contamination from chemical accidents, decision support laws and regulations are absolutely essential. Regarding this situation, this study was aimed at diagnosing problems in current chemical safety management and prevention and response system against chemical accidents through analyzing the domestic and foreign causes of chemical accidents and the accident response procedures and finally suggesting policy measures for solving those problems. In order to clarify management of soil contamination by chemical accident, this study suggests the establishment of chemical accident preparedness, response, and making of local chemical management law and policy. This law needs to be supported by a clear management framework to guide government officials and all other stakeholders in the management of soil contamination by chemical accident.
유근제,Hyunji Yoo,Jangho Lee,Eun Joo Choi,Joonhong Park 한국미생물학회 2020 The journal of microbiology Vol.58 No.2
Antibiotic resistance genes (ARGs) are emerging contaminants that pose a potential threat to human health worldwide. Urban wastewater treatment plants (WWTPs) are a main source of both antibiotic-resistant bacteria and ARGs released into the environment. Nevertheless, the propagation of ARGs and their underlying mechanisms and the dynamics of mobile genetic elements (MGEs) in WWTPs have rarely been investigated in South Korea. In this study, shotgun metagenomic analysis was used to identify comprehensive ARGs and their mechanisms, bacterial communities, and MGEs from 4 configurations with 2 activated sludge (AS) and 2 anaerobic digestion sludge (ADS) samples. A total of 181 ARG subtypes belonging to 22 ARG types were broadly detected, and the ARG abundances in the AS samples were 1.3–2.0 orders of magnitude higher than in the ADS samples. Multidrug and bacitracin resistance genes were the predominant ARG types in AS samples, followed by ARGs against sulfonamide, tetracycline, and β-lactam. However, the composition of ARG types in ADS samples was significantly changed. The abundance of multidrug and β-lactam resistance genes was drastically reduced in the ADS samples. The resistance genes of MLS were the predominant, followed by ARGs against sulfonamide and tetracycline in the ADS samples. In addition, plasmids were the dominant MGEs in the AS samples, while integrons (intI1) were the dominant MGEs in the ADS samples. These results provide valuable information regarding the prevalence of ARG types and MGEs and the difference patterns between the AS and ADS systems.
토양 및 퇴적토 환경 시료로부터 DNA 추출하는 방법에 대한 고찰
유근제,이재진,박준홍,Yoo, Keun-Je,Lee, Jae-Jin,Park, Joon-Hong 한국지하수토양환경학회 2009 지하수토양환경 Vol.14 No.3
In soil and sediment environment, microorganisms play major roles in biochemical cycles of ecological significant elements. Because of its ecological significance, microbial diversity and community structure information are useful as indexes for assessing the quality of subsurface ecological environment and bioremediation. To achieve more accurate assessment, it is requested to gain sufficient yield and purity of DNA extracted from various soil and sediment samples. Although there have been a large number of basic researches regarding soil and sediment DNA extraction methods, little guideline information is given in literature when choosing optimal DNA extraction methods for various purposes such as environmental ecology impact assessment and bioremediation capability evaluation. In this study, we performed a thorough literature review to compare the characteristics of the current DNA extraction methods from soil and sediment samples, and discussed about considerations when selecting and applying DNA extraction methods for environmental impact assessment and bioremediation capability evaluation. This review suggested that one approach is not enough to gain the suitable quantity and yield of DNA for assessing microbial diversity, community structure and population dynamics, and that a careful attention has to be paid for selecting an optimal method for individual environmental purpose. 토양미생물은 토양 및 퇴적토 환경에서 생물학적 물질순환의 중요한 역할을 맡고 있다. 이러한 중요성으로 인해 토양미생물 생태 다양성과 군집구성이 토양 및 퇴적토 생태환경과 자연저감능력을 평가하는 지표로 유용하게 쓰일 수 있다. 보다 정확한 다양성과 군집구조 분석을 위해서는 다양한 토양 및 퇴적토 시료로부터 분석에 필요한 DNA수율과 순도를 확보해야 한다. 지금까지 토양 및 퇴적토에서 DNA추출하는 방법들에 대한 다양한 기초연구가 이루어졌지만, 실제 환경생태영향평가와 분해기능평가에 사용시 DNA추출방법 선정에 대한 지침 및 정보는 매우 부족하다. 이에 본 연구에서는 문헌조사를 통해서 다양한 방법들의 토양 및 퇴적토 내 미생물 DNA 추출 특성을 비교 분석하고, 현재 주로 사용되고 있는 DNA추출방법을 토양 및 퇴적토 환경평가나 오염지하수토양 자연저감능평가에 활용 할 경우 고려해야 할 기술적 사항에 대해 고찰하였다. 본 연구를 통해 하나의 특정 추출 방법으로는 미생물다양성, 군집구성 및 개체간 상호작용에 대한 정보를 얻기에는DNA양과 순도 차원에서 충분하지 않으며, 토양 및 퇴적토 미생물 군집분석의 목적에 따라 적합한 방법의 선택이 매우 중요함을 알 수 있었다.
토양 반입 부지의 합리적 관리방안 마련을 위한 정책적 고찰
유근제 ( Keunje Yoo ),윤성지 ( Sung-ji Yoon ),김종성 ( Jongsung Kim ),황상일 ( Sang-il Hwang ) 한국환경영향평가학회 2017 환경영향평가 Vol.26 No.4
국내에서는 국토개발 목적으로 토양을 반입하고 잔토 및 사토 처리를 위해 토양을 반출하고 있으나, 토양오염에 대한 고려 없이 특정 부지로 토양이 반입되고 있는 실정이다. 이에 본 연구는 토양의 이동으로 인한 오염 확산을 방지하고 오염발생 부지에서의 정화책임을 명확하게 하기 위한 목적으로, 국내 반입토양 관리 현황을 살펴보고 국외 관련 제도를 분석하여 국내에서 토양 반입 부지의 관리체계를 효율적으로 운영하기 위한 방안을 고찰하였다. 본 연구에서는 반입되는 토양의 합리적인 이동 관리체계를 마련하기 위한 방안으로 자율적 규제, 의무적 규제, 단계적 규제를 제안하였다. 향후 국내에서 토양 반입부지에 대한 명확하고 합리적인 관리체계를 성립하기 위해서는 본 연구에서 제안한 것과 같이 현재 토양환경보전법이 가지고 있는 법·제도적 한계점을 개선하여 토양 반입 부지에 대한 규제 및 지침을 빠른 시일 내에 보완해야 할 필요성이 있다. In Korea, soil is being transported in and taken out for the purpose of national land development, but the soil is being moved without consideration of soil contamination. Therefore, this is a policy-focused study of the management system on soil moving. In this study, we analyzed current state of domestic and foreign management and suggested three alternatives for management of soil moving in 1) self-regulation, 2) obligatory regulation, 3) phase-in according to types of regulation. In order to establish a clear and reasonable management system for soil movement in the future, it is necessary to improve the legal and institutional limitations of the current soil environmental law as suggested by this study.