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개비자나무 유효성분인 homoharringtonine의 in vitro 항암활성및 in vivo 만성 독성
유귀재,조철희,이건순,류재웅,채희정 한국응용생명화학회 2008 Journal of Applied Biological Chemistry (J. Appl. Vol.51 No.2
Cell proliferation inhibitory effects of homoharringtonine (HHT), an active drug substance in Cephalotaxus koreana, against blood cancer cell line K562 were evaluated. In addition, in vivo chronic toxicity test with mouse was carried out. When K562 cell line was treated everyday for 9, 6, 3 days, IC50 values of HHT were determined as 0.27, 0.37, and 1.10 mM respectively. The anticancer activity of HHT was comparable to adriamycin, a known anticancer drug compound for blood cancer treatment. in vivo chronic toxicity test of the HHT, the number of red blood cell (RBC) showed no significant difference. From the analysis of the liver-functional enzymes in blood, all of liver damage related enzymes such as glutamate-oxalate-transferase (GOT), glutamate-pyruvatetransferase (GPT), cholesterol (Chol) and alkaline phosphatase (ALP) showed no significant change. However, from the histologic test, a neutrophil of the band type in liver tissue was observed. Cell proliferation inhibitory effects of homoharringtonine (HHT), an active drug substance in Cephalotaxus koreana, against blood cancer cell line K562 were evaluated. In addition, in vivo chronic toxicity test with mouse was carried out. When K562 cell line was treated everyday for 9, 6, 3 days, IC50 values of HHT were determined as 0.27, 0.37, and 1.10 mM respectively. The anticancer activity of HHT was comparable to adriamycin, a known anticancer drug compound for blood cancer treatment. in vivo chronic toxicity test of the HHT, the number of red blood cell (RBC) showed no significant difference. From the analysis of the liver-functional enzymes in blood, all of liver damage related enzymes such as glutamate-oxalate-transferase (GOT), glutamate-pyruvatetransferase (GPT), cholesterol (Chol) and alkaline phosphatase (ALP) showed no significant change. However, from the histologic test, a neutrophil of the band type in liver tissue was observed.
유귀재,이성동,인만진,Woo Ik Hwang,Kwang Seung Lee,Eun Sil Lee,Dong Chung Kim,채희정 한국화학공학회 2009 Korean Journal of Chemical Engineering Vol.26 No.4
Two types of sequential extraction process (SEP) for the recovery of bioactive components from Korean red ginseng were examined. As a SEP (normal SEP, nSEP), Korean red ginseng was sequentially treated using hot water and n-hexane for the successive extraction of water-soluble and lipid-soluble components. Also by changing the sequential order of solvents, an alternative SEP (reverse SEP, rSEP) in which n-hexane extraction was followed by hot water extraction, was proposed. Regardless of the sequential order of solvents, the recovery yield of acidic polysaccharide (AP) and crude saponin (CS) showed no significant change. On the other hand, in the rSEP, the lipid-soluble fraction was obtained from red ginseng with an enhanced recovery yield, four times higher than that in nSEP. Additionally, from dose-response assays to assess the effects of lipid-soluble components on the proliferation of human hepatoma (HepG2) and breast (MCF-7) cancer cell lines, it was found that hexane extract of red ginseng (HER) in rSEP has higher efficacy than the hexane extract of red ginseng marc (HERM) obtained in nSEP. This strongly suggested that rSEP would be a more attractive industrial process in terms of the efficacy of lipid-soluble extract as well as the recovery yield.
산겨릅나무 간 보호 화합물 salidroside의 추출조건 최적화
유귀재,최인욱,최상윤 한국생약학회 2022 생약학회지 Vol.53 No.4
Salidroside is a major phenolic glycoside of Acer tegmentosum (Aceraceae) and known to be a hepatoprotective compound. Extraction conditions are one of the most important factors to save time and cost from an economic point of view. For this reason, this study was conducted to optimize the extraction condition for maximum yield of salidroside. For optimization, three extraction factors such as ethanol concentration (%), extraction temperature (℃), and solvent to material ratio (mg/mL) were tested and optimized for maximum yield of salidroside using response surface methodology (RSM). The optimal condition was obtained as an ethanol concentration of 53.4%, an extraction temperature at 67.11℃ and a solvent to material ratio (mg/mL), 195.55 mg/ml. The salidroside yield under optimal conditions was found to be 1.59 mg/g dried samples, which were well-matched with the predicted value of 1.56 mg/g dried samples. These results will provide useful information about optimized extraction conditions for the development of salidroside as hepatoprotective therapeutics.
옻나무 (Rhus verniciflua Stokes) 추출물의 알코올대사 효소활성에 미치는 영향
유귀재(Guijae Yoo),김소영(Soyoung Kim),최아름(A Reum Choi),손민희(Min Hee Son),김동청(Dong Chung Kim),채희정(Hee Jeong Chae) 한국생물공학회 2009 KSBB Journal Vol.24 No.1
Alcohol oxidation activities and optimization of extraction conditions of Rrhus verniciflua Stokes (RVS) extract were evaluated for the development of a functional biomaterial for improving liver function. When alcohol oxidation activities of RVS was analyzed, the Rrhus verniciflua Stokes bark (RVSB) were higher than the Rrhus verniciflua Stokes heartwood (RVSH). Alcohol oxidation activity value of RVSB increased in a concentrationdependent manner. In the comparative analysis between Hovenia dulcis Thunb (HDT) and Alnus japonica Steud (AJS) which was reported as a alcohol oxidation material, alcohol oxidation activity is much higher than the others. The experimental conditions were optimized for alcohol oxidation-active components production from RVSB. The extraction conditions such as temperature, time, pH and particle size were performed. It was recommended to extract the alcohol oxidation-active components from RVSB by hot water (pH 7.0) at 85℃ for 8 hours.
호두 열매 추출물의 메틸글라이옥살 유도신장 세포손상 억제 효과 및 당화억제 효능
최지원,최상윤,유귀재,허진영 한국식생활문화학회 2022 韓國食生活文化學會誌 Vol.37 No.6
Methylglyoxal is a highly reactive precursor which forms advanced glycation end products (AGEs). AGEs andmethylglyoxal are known to induce various diseases such as diabetes, vascular disorders, Diabetes Mellitus (DM), andneuronal disorders. Juglans regia L is an important food commonly used worldwide, having nutritious components,including phenolic compounds. Since ancient times, Juglans regia L have been differently applied by various countries forhealth and in diverse diseases, including arthritis, asthma, skin disorders, cancer, and diabetes mellitus. However, the effectof diabetes-induced renal damage against AGEs remains unclear. This study evaluates the anti-glycation and renalprotective effects of ethanol extract of Juglans regia L against methylglyoxal-induced renal tubular epithelial cell death. Exposure to methylglyoxal resulted in reduced cell viability in NRK-52E cells, but co-treatment with Juglans regia L extractssignificantly increased the cell viability. In addition, we examined the anti-glycation effect of Juglans regia L extracts. Compared to the positive control aminoguanidine and Alagebrium, treatment with Juglans regia L extracts significantlyinhibited the formation of AGEs, collagen cross-linking, and breaking collagen cross-linking. Taken together, our resultsindicate that Juglans regia L is a potential therapeutic agent for regulating diabetic complications by exerting anti-glycationand renal protective activities.
장현석,강교빈,유귀재,권은아,윤준원,강병철,홍인기,김승현,성상현,조현종,장영표,양희정 대한화학회 2018 Bulletin of the Korean Chemical Society Vol.39 No.6
Cnidium rhizome from Cnidium officinale Makino and Ligusticum chuanxiong Hort (Umbelliferae) is traditionally used to treat pain in East Asian countries. Senkyunolide A and Z?ligustilide are phthalic anhydride derivatives that are the bioactive components of cnidium rhizome and chemical markers of the two species; they are also susceptible to external stimuli. In this study, we developed a method to identify two chemical markers using high?performance liquid chromatography (HPLC) with an ultraviolet detector (UVD); the contents of senkyunolide A and Z?ligustilide in cnidium rhizome from different regions were quantified. The stabilities of the compounds were tested at two different temperatures over 12 months. An HPLC?UVD system was used to identify and quantify senkyunolide A and Z?ligustilide in 10 C. officinale samples and 6 L. chuanxiong samples from China and Korea. The long?term stability of the C. officinale samples in dark conditions at 4 and 25 °C was also tested. The calibration curves for the two chemical markers showed high linearity (R2 = 1.0000), the precision was high (relative standard deviation (RSD) <4.17%), and the recoveries were within the range of 95?105%. The contents were mostly independent of the geographical origin and species, and >10% of the compounds degraded after storage at 4 and 25 °C for 6 months. A method was developed to rapidly identify two chemical markers in cnidium rhizome with good linearity, precision, and accuracy using HPLC?UVD. These markers will be utilized for the quality control or evaluation of herbal preparations containing cnidium rhizome.
포도 송이가지를 이용한 레스베라트롤의 추출 및 항산화 활성
조철희,김소영,유귀재,손민희,박근형,임병락,김동청,채희정 한국응용생명화학회 2008 Journal of Applied Biological Chemistry (J. Appl. Vol.51 No.1
The extraction conditions for resveratrol production from grape fruit stem, which is a by-product of grape processing, were optimized to develop high-functional grape-based products. Additionally, the bioefficacy of grape fruit stem extract (GFSE) as an antioxidant agent was evaluated. Resveratrol was extracted using various experimental conditions such as extractant type, extractant concentration, raw material-extractant ratio, extraction time and temperature, and the results were analyzed using a statistical program (SPSS). The resveratrol yield was the highest when 80% ethanol with a raw-material-extractant ratio of 1 : 10 (w/v) was used. In addition, the optimal temperature and time were selected as 60oC and 90 min, respectively. When the antioxidant activity was analyzed and expressed as DPPH radical scavenging activity and SOD-like activity, the antioxidant activity of GFSE was higher than that of BHT, BHA and L-ascorbic acid. Finally, it was found that GFSE could be used as a raw material for the production of high antioxidant agents. The extraction conditions for resveratrol production from grape fruit stem, which is a by-product of grape processing, were optimized to develop high-functional grape-based products. Additionally, the bioefficacy of grape fruit stem extract (GFSE) as an antioxidant agent was evaluated. Resveratrol was extracted using various experimental conditions such as extractant type, extractant concentration, raw material-extractant ratio, extraction time and temperature, and the results were analyzed using a statistical program (SPSS). The resveratrol yield was the highest when 80% ethanol with a raw-material-extractant ratio of 1 : 10 (w/v) was used. In addition, the optimal temperature and time were selected as 60oC and 90 min, respectively. When the antioxidant activity was analyzed and expressed as DPPH radical scavenging activity and SOD-like activity, the antioxidant activity of GFSE was higher than that of BHT, BHA and L-ascorbic acid. Finally, it was found that GFSE could be used as a raw material for the production of high antioxidant agents.